RchiOBHm_Chr3g0476881

pentatricopeptide repeat-containing protein At5g06400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007077
Physical Location & Seq
Reverse (-)
22839415 .. 22842762
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3102 bp
ATGCGAGGCTTATCCACCATATTACAGTCCTTGTGTTGGAGCTCAACCTCTAAAACCCTTGATTTCCGATTCTCAAAATTTCATAAGATTGATAACTTTGCGACGCTCACTAAGCCATCTACTAAGCCCAACAAAGCCCACTTGAAACAACAACAAGAACCGAGTTCGTTTGGATCGCTGTTCAGTGAAATAACTGAGATTTTAGGGGCTGAACATGTCACTCTGGATAAAACCCCATCTGGGTTTTTTATTTGCAAGGAAACCCATCTTGGGGTTGGTGAGGTTGGGCAAGAGATTGCTAAGGTGAATGCACTGGGAGAAAAGGAAGATATAACTGTTGTGGAGGAAACCAGATTCGAAAGCGTAGCTGAAACTGATGTTAGTCCAGTAGTTCATGAGGTTACGAACATTGTCAGGGCGGAAAATGGTTTGGTTTCGATGGAGGAGCGGTTAGACGAGTTGGGTTTTCAGTTAGATTCAGAGATTGTTGACAAAGTTCTGAAGAGGTGCTTTAAAGTGCCACATTTGGCTTTAAGGTTCTTTGATTGGGTGAAACTGAAAGTATGGTTCTGTCACACGACTAAGACTTATAATACCGTGCTGTGTATAGCTGGGGAGGCAAGAGAGTTCGGGCTGGTGGAGAAGATGGTGGAGGAGATGGAAAGGAACTTGTGCGAGAAAGATGTTAAGACCTGGACCATTCTTATCCAGATGTATGGGAAGGCTAAGTTAGTTGGAAGAGCCTTGTTAGTCTATGAAAAGATGAAGAAATGCGGTTTTGAACCAGACTCCGTGGTTTTCAGAATGATGATACGTGCACTTTGTGCTGCTGGAAAAGCCGACGTGGCAATGGAATTTTACAAGGAAATGGTACAGAAGGACATTGGACTTGATATCAATATGTATAAGCTGTTATTGAATGGCATAGCTAGCTACGGAGATACTGCTGCTGTGGGCTTGGTTTCAGATGACATGATGAGAGTCTCACAGATTCCTGAACATGCGGTTTATGGTTGTGTCCTGAAGTCTTTCTGTATCTCTGGTAGAATCAGAGAAGCTTTGGAATATATTCGTGAGCTCAAGAATAAAGATGTTATGCTTCTCCCTGAATATTTTGAGACTTTGGTGAAAGGACTGTGCAGGGCTGATAGAATTGCAGATGCTTTGGAGATTTTGGATATTATGAAGAAAAGAGATTTTGTTGATGGAAATGTGTATGGGATTATCATCAATGGATACTTGAGAAAAAATGATGTTTCTAGAGCACTTGATCTGTTTCGTAGTATGAAAGAATGTGGATATTTGCCTACTACTTCTACCTACACTGAACTGATGCAGCGCCTCTTCAATGAGAATGAGTATCAACAAGGCTGTGAGCTGTATGATGAGATGCTTGAAAGAGGAGTTGAGCCAGATAGTGTGGCCGTTACAGCCATGGTTGCAGGCCACGTCCGCCAAAACCATATATCTGAAGCATGGAAAGTATTTAATGATATGAAGGACAAAGGCATTAAGCCCACTTCAAAGTTGTATTTAGTATTTATCAAGGAGCTTTGCAGAATTTCAAGGACAGATGAGATTCTGAAGGTCTTGGTTCATATGCGGGCATCTGATATGCGCATTAAAGATGACACATTCAATTGGGTTGTACTTCATATGGAGAAAAAGGGAGAGATGGATAGCCTTGAAAAAGTAAAGCAGATGCAAAGTCTTTATAAATTGCAGCCTCATGATGGAGAGGTATCAAGCAGTGATGCATCTAGTGGAGAGAGGACCAATACAGTGTTGGAGTTGCTACCAAAGAAGATGGATCCCTTATTGAATTCTGATGCTTATGATGACAAGGATTTGCAAGAAATTTGTAAAATTCTGTCCTCTTCAACTGACTGGTGCTTCATTCAAGAAGTTTTGGAGAAGTCTTCTGTTGATTACACTCCAGGACTCGTTCTGGAAATTTTGAGGTATCACAGTGGGCATGGTTCGGTAGCATTACAGTTTTTCTCGTGGGTTGGAAAGCAATCTGGTTATTGCCACACTACTGATACTTACAACATGGCTATCAAAATTGCAGGGCGTGGGAAAGATTTCAAGCACATGAGGAACCTTTTCTATGAAATGAGGAGAAACAGTTTCTCAATAACACAAGATACATGGACAATAATGATAATGCAATATGGTCGAACAGGTCTCACAGAGATTGCTCTTCAGTTTTTCAGAGAGATGAAAAGTAATTGCAACCCAACTGGCAGTACCTATAAGTATTTGATCATATCTCTTTGTGGGAGAAAAGGTCGAAGGGTAGATGAAGCAATTAAAATATTCCAGGAGATGATCCGTGCTGCACATATACCTGACAAAGAGTTGGTTGAAACTTACGTTGGTTGTTTATGTGAAGTTGGTAAGCTGTCAGATGCTAGAAGATGCATAGATTCTCTTTCCAAAGTTGGTTTTACCACCTCTCTTGGTTGTTCCTTGTACATTAGGGCTCTTTGTCGAGCTGGGAAGTTACAAGAAGCTTCTGCGTTGATGGATGATATTGGGGAAGAGCGATCCACATTGGATCGGTATACTTATGGGAGCTTTGTTCATGGACTTTTGCGGAGTGGACAAGTAGAAGAAGCACTGGCCAAGGTGGAGTCTATGAAGCAGGCTGGCATGAAGCCAACCGTTCATGTTTATACATCCTTAATAGTTCATTTCTTTAAGGAGAAGCAGATCAAAAGGGCATTAGAAATTTTCAATGAAATGCAACAGGAAGGATGTGAACCGACGGTAGTTACTTATTCCGCGCTTATCCGAGGGTACATGAACATGGGGATGATTGCTGAAGCTTGGAGTGTCTTTCACAACATGAAACTGAAAGGGCCATTGCCGGATTTCAAAACTTATTCAATGTTCCTTCATTGTCTTTGTAAGGCAGGGAAATCAGAAGAAGCCATGCAGCTTATATCTGAAATGTTGAACAGTGGGATTGTTCCCAGTGTTGTCAATTTTCGAACTGTATTTTACGGACTTAACCGAGAAGGTAAGCAAGATTTGGCTCGCATTGTAATGGAACAGAAGTCATCTTTGATTAGAAAACGTAAGTTCTTAATATCATGA

Protein Analysis

1033

Amino Acids

117.69

Weight (kDa)

7.07

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 227 - 275 1.6e-11 PPR repeat family
PPR_1 PF12854 259 - 291 1.7e-06 PPR repeat
PPR_2 PF13041 406 - 446 1.3e-08 PPR repeat family
PPR_long PF17177 414 - 549 2.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 436 - 482 1.3e-08 PPR repeat family
PPR_2 PF13041 471 - 520 3.3e-12 PPR repeat family
PPR_2 PF13041 717 - 760 1.2e-06 PPR repeat family
TPR_24 PF23276 832 - 950 7.7e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 857 - 897 9e-06 PPR repeat family
PPR_long PF17177 867 - 995 6.2e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 879 - 935 1.5e-13 Pentatricopeptide repeat domain
PPR_2 PF13041 888 - 936 3.3e-15 PPR repeat family
PPR_1 PF12854 920 - 952 2.1e-07 PPR repeat
PPR_2 PF13041 923 - 972 2.5e-14 PPR repeat family
PPR PF01535 926 - 955 1e-05 PPR repeat
PPR_1 PF12854 954 - 986 2.6e-07 PPR repeat
PPR PF01535 962 - 991 8.9e-06 PPR repeat
PPR_2 PF13041 962 - 1005 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 591, 1716
Acc16I TGCGCA 1 cut(s) 1619
AccBSI CCGCTC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 2567
AccII CGCG 1 cut(s) 2789
AciI CCGC 8 cut(s) 419, 448, 774, 1004, 1453, 1603, 2599, 2787
AclWI GGATC 6 cut(s) 181, 1805, 1818, 2326, 2544, 2568
AcoI YGGCCR 2 cut(s) 1422, 2625
AcsI RAATTY 8 cut(s) 77, 854, 1560, 1822, 1857, 1866, 1953, 2733
AcuI CTGAAG 6 cut(s) 521, 1043, 1491, 1604, 2189, 2847
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 5 cut(s) 873, 1650, 2251, 2477, 2804
AfiI CCNNNNNNNGG 5 cut(s) 36, 240, 270, 271, 1733
AflIII ACRYGT 1 cut(s) 214
AjiI CACGTC 2 cut(s) 844, 1450
AjnI CCWGG 3 cut(s) 692, 1936, 2322
AjuI GAANNNNNNNTTGG 4 cut(s) 252, 284, 2361, 2393
Alw21I GWGCWC 4 cut(s) 44, 820, 1080, 1267
Alw26I GTCTC 3 cut(s) 988, 1112, 2192
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 6 cut(s) 181, 1805, 1818, 2326, 2544, 2568
AoxI GGCC 4 cut(s) 1422, 1444, 2625, 2864
ApaLI GTGCAC 1 cut(s) 816
ApeKI GCWGC 6 cut(s) 827, 947, 1336, 1723, 2339, 2941
ApoI RAATTY 8 cut(s) 77, 854, 1560, 1822, 1857, 1866, 1953, 2733
Asp700I GAANNNNTTC 4 cut(s) 1028, 1068, 2084, 2105
AspLEI GCGC 3 cut(s) 1341, 1620, 2791
AspS9I GGNCC 3 cut(s) 696, 1773, 2864
AsuHPI GGTGA 4 cut(s) 290, 316, 562, 1138
AsuII TTCGAA 2 cut(s) 357, 2995
AsuNHI GCTAGC 1 cut(s) 929
AvaII GGWCC 2 cut(s) 696, 1773
BaeGI GKGCMC 1 cut(s) 820
BalI TGGCCA 1 cut(s) 2627
BamHI GGATCC 1 cut(s) 1810
BanII GRGCYC 3 cut(s) 44, 1080, 2488
BauI CACGAG 1 cut(s) 2002
BbsI GAAGAC 1 cut(s) 1911
Bbv12I GWGCWC 4 cut(s) 44, 820, 1080, 1267
BbvI GCAGC 6 cut(s) 814, 934, 1348, 1735, 2326, 2953
BceAI ACGGC 1 cut(s) 1409
BcgI CGANNNNNNTGC 2 cut(s) 2159, 2193
BciT130I CCWGG 3 cut(s) 694, 1938, 2324
BciVI GTATCC 1 cut(s) 1229
BclI TGATCA 1 cut(s) 2265
BcoDI GTCTC 3 cut(s) 988, 1112, 2192
BfaI CTAG 4 cut(s) 930, 1260, 1761, 2415
BfoI RGCGCY 1 cut(s) 1342
BfuI GTATCC 1 cut(s) 1229
BglI GCCNNNNNGGC 1 cut(s) 845
BisI GCNGC 6 cut(s) 828, 948, 1337, 1724, 2340, 2942
BlsI GCNGC 6 cut(s) 829, 949, 1338, 1725, 2341, 2943
Bme1390I CCNGG 3 cut(s) 694, 1938, 2324
Bme18I GGWCC 2 cut(s) 696, 1773
BmgBI CACGTC 2 cut(s) 844, 1450
BmgT120I GGNCC 3 cut(s) 696, 1773, 2864
BmiI GGNNCC 2 cut(s) 1812, 2102
BmrFI CCNGG 3 cut(s) 694, 1938, 2324
BmrI ACTGGG 2 cut(s) 323, 2973
BmtI GCTAGC 1 cut(s) 933
BmuI ACTGGG 2 cut(s) 323, 2973
BpiI GAAGAC 1 cut(s) 1911
BpmI CTGGAG 1 cut(s) 1920
Bpu10I CCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 2 cut(s) 357, 2995
BpuEI CTTGAG 2 cut(s) 1064, 1261
BsaAI YACGTR 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 1226
BsaI GGTCTC 1 cut(s) 2192
BsaJI CCNNGG 4 cut(s) 792, 1434, 2628, 2797
BsaXI ACNNNNNCTCC 6 cut(s) 434, 464, 2113, 2143, 2275, 2305
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 240, 270, 271, 1733
Bse1I ACTGG 6 cut(s) 318, 386, 1892, 2248, 2628, 2979
Bse3DI GCAATG 2 cut(s) 855, 2867
Bse8I GATNNNNATC 1 cut(s) 1226
BseBI CCWGG 3 cut(s) 694, 1938, 2324
BseDI CCNNGG 4 cut(s) 792, 1434, 2628, 2797
BseGI GGATG 4 cut(s) 2536, 2681, 2765, 2823
BseJI GATNNNNATC 1 cut(s) 1226
BseLI CCNNNNNNNGG 5 cut(s) 36, 240, 270, 271, 1733
BseMI GCAATG 2 cut(s) 855, 2867
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 6 cut(s) 318, 386, 1892, 2248, 2628, 2979
BseRI GAGGAG 4 cut(s) 458, 668, 1416, 2134
BseSI GKGCMC 1 cut(s) 820
BseXI GCAGC 6 cut(s) 814, 934, 1348, 1735, 2326, 2953
BseYI CCCAGC 2 cut(s) 611, 2498
BsgI GTGCAG 2 cut(s) 1159, 2325
Bsh1236I CGCG 1 cut(s) 2789
BshFI GGCC 4 cut(s) 1424, 1446, 2627, 2866
BsiHKAI GWGCWC 4 cut(s) 44, 820, 1080, 1267
BsiSI CCGG 1 cut(s) 2873
BslI CCNNNNNNNGG 5 cut(s) 36, 240, 270, 271, 1733
BsmAI GTCTC 3 cut(s) 988, 1112, 2192
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 4 cut(s) 1424, 1446, 2627, 2866
Bso31I GGTCTC 1 cut(s) 2192
Bsp119I TTCGAA 2 cut(s) 357, 2995
Bsp1286I GDGCHC 5 cut(s) 44, 820, 1080, 1267, 2488
Bsp1407I TGTACA 1 cut(s) 2475
Bsp143I GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
Bsp19I CCATGG 1 cut(s) 1434
BspACI CCGC 8 cut(s) 419, 448, 774, 1004, 1453, 1603, 2599, 2787
BspANI GGCC 4 cut(s) 1424, 1446, 2627, 2866
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 1 cut(s) 2789
BspHI TCATGA 3 cut(s) 394, 1729, 3098
BspLI GGNNCC 2 cut(s) 1812, 2102
BspOI GCTAGC 1 cut(s) 933
BspPI GGATC 6 cut(s) 181, 1805, 1818, 2326, 2544, 2568
BspQI GCTCTTC 3 cut(s) 733, 2208, 2538
BspT104I TTCGAA 2 cut(s) 357, 2995
BspTNI GGTCTC 1 cut(s) 2192
BsrBI CCGCTC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 855, 2867
BsrGI TGTACA 1 cut(s) 2475
BsrI ACTGG 6 cut(s) 318, 386, 1892, 2248, 2628, 2979
BssECI CCNNGG 4 cut(s) 792, 1434, 2628, 2797
BssMI GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
BssNAI GTATAC 1 cut(s) 2568
BssSI CACGAG 1 cut(s) 2002
BssT1I CCWWGG 2 cut(s) 1434, 2628
Bst1107I GTATAC 1 cut(s) 2568
Bst2BI CACGAG 1 cut(s) 2002
Bst2UI CCWGG 3 cut(s) 694, 1938, 2324
Bst6I CTCTTC 6 cut(s) 497, 733, 1349, 1882, 2208, 2538
BstAPI GCANNNNNTGC 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 2475
BstBAI YACGTR 1 cut(s) 815
BstBI TTCGAA 2 cut(s) 357, 2995
BstC8I GCNNGC 6 cut(s) 931, 1444, 1605, 2649, 2653, 3043
BstDEI CTNAG 6 cut(s) 111, 123, 195, 300, 582, 726
BstDSI CCRYGG 2 cut(s) 792, 1434
BstF5I GGATG 4 cut(s) 2536, 2681, 2765, 2823
BstFNI CGCG 1 cut(s) 2789
BstH2I RGCGCY 1 cut(s) 1342
BstHHI GCGC 3 cut(s) 1341, 1620, 2791
BstKTI GATC 8 cut(s) 176, 1273, 1813, 2268, 2334, 2552, 2563, 2718
BstMAI GTCTC 3 cut(s) 988, 1112, 2192
BstMBI GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
BstMWI GCNNNNNNNGC 9 cut(s) 112, 617, 824, 836, 845, 930, 1430, 1439, 1452
BstNI CCWGG 3 cut(s) 694, 1938, 2324
BstNSI RCATGY 2 cut(s) 218, 1004
BstSCI CCNGG 3 cut(s) 692, 1936, 2322
BstSLI GKGCMC 1 cut(s) 820
BstUI CGCG 1 cut(s) 2789
BstV1I GCAGC 6 cut(s) 814, 934, 1348, 1735, 2326, 2953
BstV2I GAAGAC 1 cut(s) 1911
BstX2I RGATCY 1 cut(s) 1810
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 1 cut(s) 1810
BstZ17I GTATAC 1 cut(s) 2568
BsuI GTATCC 1 cut(s) 1229
BsuRI GGCC 4 cut(s) 1424, 1446, 2627, 2866
BtgI CCRYGG 2 cut(s) 792, 1434
BtrI CACGTC 2 cut(s) 844, 1450
BtsCI GGATG 4 cut(s) 2536, 2681, 2765, 2823
BtsI GCAGTG 1 cut(s) 1756
BtsIMutI CAGTG 9 cut(s) 190, 311, 1323, 1756, 1788, 1975, 2621, 2971, 2986
Cac8I GCNNGC 6 cut(s) 931, 1444, 1605, 2649, 2653, 3043
CciI TCATGA 3 cut(s) 394, 1729, 3098
CfoI GCGC 3 cut(s) 1341, 1620, 2791
Cfr13I GGNCC 3 cut(s) 696, 1773, 2864
CseI GACGC 1 cut(s) 112
Csp6I GTAC 5 cut(s) 872, 1649, 2250, 2476, 2803
CspCI CAANNNNNGTGG 2 cut(s) 2443, 2478
CviQI GTAC 5 cut(s) 872, 1649, 2250, 2476, 2803
DdeI CTNAG 6 cut(s) 111, 123, 195, 300, 582, 726
DpnI GATC 8 cut(s) 175, 1272, 1812, 2267, 2333, 2551, 2562, 2717
DpnII GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
DraI TTTAAA 1 cut(s) 514
DraIII CACNNNGTG 1 cut(s) 824
EaeI YGGCCR 2 cut(s) 1422, 2625
Eam1104I CTCTTC 6 cut(s) 497, 733, 1349, 1882, 2208, 2538
EarI CTCTTC 6 cut(s) 497, 733, 1349, 1882, 2208, 2538
EciI GGCGGA 2 cut(s) 434, 1442
Ecl136II GAGCTC 2 cut(s) 42, 1078
Eco130I CCWWGG 2 cut(s) 1434, 2628
Eco24I GRGCYC 3 cut(s) 44, 1080, 2488
Eco31I GGTCTC 1 cut(s) 2192
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 2 cut(s) 696, 1773
Eco53kI GAGCTC 2 cut(s) 42, 1078
Eco57I CTGAAG 6 cut(s) 521, 1043, 1491, 1604, 2189, 2847
EcoICRI GAGCTC 2 cut(s) 42, 1078
EcoRI GAATTC 1 cut(s) 1822
EcoRII CCWGG 3 cut(s) 692, 1936, 2322
EcoRV GATATC 1 cut(s) 895
EcoT14I CCWWGG 2 cut(s) 1434, 2628
EcoT22I ATGCAT 2 cut(s) 1759, 2426
EcoT38I GRGCYC 3 cut(s) 44, 1080, 2488
ErhI CCWWGG 2 cut(s) 1434, 2628
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FauI CCCGC 1 cut(s) 1596
FauNDI CATATG 2 cut(s) 1599, 1656
FbaI TGATCA 1 cut(s) 2265
FblI GTMKAC 1 cut(s) 2567
Fnu4HI GCNGC 6 cut(s) 828, 948, 1337, 1724, 2340, 2942
FokI GGATG 4 cut(s) 2543, 2668, 2772, 2830
FriOI GRGCYC 3 cut(s) 44, 1080, 2488
Fsp4HI GCNGC 6 cut(s) 828, 948, 1337, 1724, 2340, 2942
FspAI RTGCGCAY 1 cut(s) 1619
FspBI CTAG 4 cut(s) 930, 1260, 1761, 2415
FspI TGCGCA 1 cut(s) 1619
GlaI GCGC 3 cut(s) 1340, 1619, 2790
GluI GCNGC 6 cut(s) 828, 948, 1337, 1724, 2340, 2942
GsaI CCCAGC 2 cut(s) 615, 2502
GsuI CTGGAG 1 cut(s) 1920
HaeII RGCGCY 1 cut(s) 1342
HaeIII GGCC 4 cut(s) 1424, 1446, 2627, 2866
HapII CCGG 1 cut(s) 2873
HgaI GACGC 1 cut(s) 112
HhaI GCGC 3 cut(s) 1341, 1620, 2791
Hin6I GCGC 3 cut(s) 1339, 1618, 2789
HinP1I GCGC 3 cut(s) 1339, 1618, 2789
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 3 cut(s) 1056, 2514, 2829
HpaII CCGG 1 cut(s) 2873
HphI GGTGA 4 cut(s) 290, 316, 562, 1138
Hpy166II GTNNAC 5 cut(s) 490, 818, 2568, 2606, 2765
Hpy8I GTNNAC 5 cut(s) 490, 818, 2568, 2606, 2765
Hpy99I CGWCG 3 cut(s) 106, 845, 2773
HpyAV CCTTC 8 cut(s) 715, 871, 1492, 1579, 2289, 2750, 2909, 3017
HpyCH4IV ACGT 5 cut(s) 814, 843, 1449, 2376, 3082
HpyF10VI GCNNNNNNNGC 9 cut(s) 112, 617, 824, 836, 845, 930, 1430, 1439, 1452
HpyF3I CTNAG 6 cut(s) 111, 123, 195, 300, 582, 726
HpySE526I ACGT 5 cut(s) 814, 843, 1449, 2376, 3082
HspAI GCGC 3 cut(s) 1339, 1618, 2789
Ksp22I TGATCA 1 cut(s) 2265
Kzo9I GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
LguI GCTCTTC 3 cut(s) 733, 2208, 2538
LmnI GCTCC 4 cut(s) 39, 445, 1549, 2577
Lsp1109I GCAGC 6 cut(s) 814, 934, 1348, 1735, 2326, 2953
MaeI CTAG 4 cut(s) 930, 1260, 1761, 2415
MaeII ACGT 5 cut(s) 814, 843, 1449, 2376, 3082
MaeIII GTNAC 6 cut(s) 217, 400, 572, 1426, 2505, 2776
MalI GATC 8 cut(s) 175, 1272, 1812, 2267, 2333, 2551, 2562, 2717
MbiI CCGCTC 1 cut(s) 448
MboI GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
MfeI CAATTG 1 cut(s) 1639
MflI RGATCY 1 cut(s) 1810
MhlI GDGCHC 5 cut(s) 44, 820, 1080, 1267, 2488
MlsI TGGCCA 1 cut(s) 2627
MluNI TGGCCA 1 cut(s) 2627
MlyI GAGTC 4 cut(s) 782, 990, 1935, 2645
MmeI TCCRAC 4 cut(s) 17, 715, 1767, 1990
Mox20I TGGCCA 1 cut(s) 2627
Mph1103I ATGCAT 2 cut(s) 1759, 2426
MroXI GAANNNNTTC 4 cut(s) 1028, 1068, 2084, 2105
MscI TGGCCA 1 cut(s) 2627
MslI CAYNNNNRTG 3 cut(s) 2810, 2816, 3050
Msp20I TGGCCA 1 cut(s) 2627
MspI CCGG 1 cut(s) 2873
MspR9I CCNGG 3 cut(s) 694, 1938, 2324
MunI CAATTG 1 cut(s) 1639
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 3 cut(s) 694, 1938, 2324
MvnI CGCG 1 cut(s) 2789
MwoI GCNNNNNNNGC 9 cut(s) 112, 617, 824, 836, 845, 930, 1430, 1439, 1452
NcoI CCATGG 1 cut(s) 1434
NdeI CATATG 2 cut(s) 1599, 1656
NdeII GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
NheI GCTAGC 1 cut(s) 929
NlaIV GGNNCC 2 cut(s) 1812, 2102
NmuCI GTSAC 2 cut(s) 217, 572
NsbI TGCGCA 1 cut(s) 1619
NsiI ATGCAT 2 cut(s) 1759, 2426
NspI RCATGY 2 cut(s) 218, 1004
NspV TTCGAA 2 cut(s) 357, 2995
PagI TCATGA 3 cut(s) 394, 1729, 3098
PciI ACATGT 1 cut(s) 214
PciSI GCTCTTC 3 cut(s) 733, 2208, 2538
PctI GAATGC 1 cut(s) 313
PdmI GAANNNNTTC 4 cut(s) 1028, 1068, 2084, 2105
PfeI GAWTC 7 cut(s) 69, 354, 476, 991, 1047, 1579, 2429
PfoI TCCNGGA 2 cut(s) 1936, 2322
PkrI GCNGC 6 cut(s) 829, 949, 1338, 1725, 2341, 2943
PleI GAGTC 4 cut(s) 782, 989, 1935, 2644
PpsI GAGTC 4 cut(s) 782, 989, 1935, 2644
Ppu21I YACGTR 1 cut(s) 815
PscI ACATGT 1 cut(s) 214
PsiI TTATAA 2 cut(s) 591, 1716
Psp124BI GAGCTC 2 cut(s) 44, 1080
Psp6I CCWGG 3 cut(s) 692, 1936, 2322
PspFI CCCAGC 2 cut(s) 611, 2498
PspGI CCWGG 3 cut(s) 692, 1936, 2322
PspN4I GGNNCC 2 cut(s) 1812, 2102
PspPI GGNCC 3 cut(s) 696, 1773, 2864
PsuI RGATCY 1 cut(s) 1810
RsaI GTAC 5 cut(s) 873, 1650, 2251, 2477, 2804
RsaNI GTAC 5 cut(s) 872, 1649, 2250, 2476, 2803
RseI CAYNNNNRTG 3 cut(s) 2810, 2816, 3050
SacI GAGCTC 2 cut(s) 44, 1080
SapI GCTCTTC 3 cut(s) 733, 2208, 2538
SatI GCNGC 6 cut(s) 828, 948, 1337, 1724, 2340, 2942
Sau3AI GATC 8 cut(s) 173, 1270, 1810, 2265, 2331, 2549, 2560, 2715
Sau96I GGNCC 3 cut(s) 696, 1773, 2864
SchI GAGTC 4 cut(s) 782, 990, 1935, 2645
ScrFI CCNGG 3 cut(s) 694, 1938, 2324
SduI GDGCHC 5 cut(s) 44, 820, 1080, 1267, 2488
SfuI TTCGAA 2 cut(s) 357, 2995
SinI GGWCC 2 cut(s) 696, 1773
SmiMI CAYNNNNRTG 3 cut(s) 2810, 2816, 3050
SmlI CTYRAG 2 cut(s) 1079, 1240
SmoI CTYRAG 2 cut(s) 1079, 1240
SsiI CCGC 8 cut(s) 419, 448, 774, 1004, 1453, 1603, 2599, 2787
SspI AATATT 2 cut(s) 1112, 2319
SspMI CTAG 4 cut(s) 930, 1260, 1761, 2415
SstI GAGCTC 2 cut(s) 44, 1080
StyD4I CCNGG 3 cut(s) 692, 1936, 2322
StyI CCWWGG 2 cut(s) 1434, 2628
TaiI ACGT 5 cut(s) 817, 846, 1452, 2379, 3085
TaqI TCGA 6 cut(s) 357, 437, 2179, 2293, 2494, 2995
TatI WGTACW 2 cut(s) 1648, 2475
TfiI GAWTC 7 cut(s) 69, 354, 476, 991, 1047, 1579, 2429
TscAI CASTG 9 cut(s) 190, 318, 1330, 1756, 1788, 1975, 2628, 2971, 2986
TseFI GTSAC 2 cut(s) 217, 572
TseI GCWGC 6 cut(s) 827, 947, 1336, 1723, 2339, 2941
Tsp45I GTSAC 2 cut(s) 217, 572
TspGWI ACGGA 4 cut(s) 781, 951, 2324, 3024
TspRI CASTG 9 cut(s) 190, 318, 1330, 1756, 1788, 1975, 2628, 2971, 2986
VneI GTGCAC 1 cut(s) 816
VpaK11BI GGWCC 2 cut(s) 696, 1773
XapI RAATTY 8 cut(s) 77, 854, 1560, 1822, 1857, 1866, 1953, 2733
XbaI TCTAGA 1 cut(s) 1259
XceI RCATGY 2 cut(s) 218, 1004
XcmI CCANNNNNNNNNTGG 2 cut(s) 272, 1783
XmiI GTMKAC 1 cut(s) 2567
XmnI GAANNNNTTC 4 cut(s) 1028, 1068, 2084, 2105
XspI CTAG 4 cut(s) 930, 1260, 1761, 2415
Zsp2I ATGCAT 2 cut(s) 1759, 2426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.