RchiOBHm_Chr3g0481051

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011578
Physical Location & Seq
Forward (+)
27079727 .. 27086223
6497 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1641 bp
ATGGCCGACCAGAGAAAAGGTAGCCTTGAAGATACACCAACATGGGCTGTATCAGTATTTGCTTTGTTTTTCTTCGTGCTATCCTTTATAATCGACGATGGTCTTCATCGTCTAACGAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTGAATAGGGCTTTGAAGAAGTTCAAAACAGAAATGATGCAACTGGGTTTCTTATCACTGCTTCTTACTCTGTCGGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAACTTCCAGCTTCAAATACCAACTTCTCTAAGGAAGTCACCACTCAAACTTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAAGGGAAGGAATCCTGCAGCTAAACATCTTTATATCGGTCTTGGCAGTGTTTCATGTTCTCTACTGCATCTTAACTATGTTTCTTGGGATGGCCAAGGTTTGTTTTGCTCGGCAGTTTGGCGGCTCAATATCAAAAGTAGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACATCAAAGAAGGTAGCAGCTTCAATTTCCTGAACTTCCTTGCTAGAGCTTTCGATGACGATTTTGAGCAAGTAGTTGGAATCAAATTAATAACATCTGGTGTCATTTCTTTAATCAAGATTGTTCTACTCGTGGGAACAAAGCTGCAAGTCGTGATAACTAAAATGTGCGCGGAGAGTTGTAAGAAGAATCCTGTGATTCGCGGAAGCTTTGTGGTGGAGCTCAACAATGATTTATTCTGGTTTGGTCAACCCAATTGGCTTCTTCATCTCCTACAGTTTGTCCTAATCCAGATTGAGTATGGCCAAAGTTCATGCTTTAATCGAAGAACAAAAGATGTTATCATAAAGAGTACTATGGGAATTGTTGTGCAGTTGATTTGTGGTTATGTAACCCTCCCTCTTTATGCACTGGTTAACCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTAAAAAACTGGCACAAGAATGCAAGCCACAGACTATCGAAGAACGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACATTTTTATTCAACAGATGACTCCTCAATTCTAGACAATGAATGTAGCTACAAAACTCCTAATCTAACCATCGTGAGAAGCTCTTCTTCTACCTCCGAGATAATTGAAGAGCAGGTTCCACCCGGTTTAACTCCGAAGAATGGATCCAGTACTTCAAGAAAATCATCAAATTCTGCACAATTTTATTCAACTGATGACTCCTCAATTCCAGACAATGAATGTAGCTACAAAACTCCTCATCCCACCATTGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCACGTTTCACCCGGTTTAACTCCAAACCTGGATACTACAAGCTTACCAACTCAAGAAATCACCAGAGACGAAGAGAAACCCAACATCATCAACAAAATAATCTATGACGGTGAGATCTCATTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATTAGCAAGAGAAGTGGAGAAATCACTTCAATAATTGAAGAAGAAGTTAACTTCGATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

546

Amino Acids

60.66

Weight (kDa)

8.18

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 169 3.2e-45 Mlo family
Mlo PF03094 169 - 225 5.3e-18 Mlo family
Mlo PF03094 230 - 383 7.3e-45 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 1240
AccII CGCG 2 cut(s) 755, 786
AciI CCGC 4 cut(s) 318, 528, 755, 786
AclWI GGATC 4 cut(s) 1098, 1111, 1275, 1288
AcoI YGGCCR 4 cut(s) 3, 498, 579, 886
AcsI RAATTY 2 cut(s) 1129, 1306
AfaI GTAC 3 cut(s) 228, 937, 1288
AfiI CCNNNNNNNGG 3 cut(s) 153, 154, 1277
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 1 cut(s) 1452
AleI CACNNNNGTG 1 cut(s) 1385
Alw21I GWGCWC 3 cut(s) 807, 1044, 1428
Alw26I GTCTC 3 cut(s) 253, 313, 1485
AlwI GGATC 4 cut(s) 1098, 1111, 1275, 1288
AoxI GGCC 5 cut(s) 3, 498, 579, 886, 1054
ApeKI GCWGC 3 cut(s) 424, 600, 727
ApoI RAATTY 2 cut(s) 1129, 1306
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 3 cut(s) 167, 1219, 1396
AspLEI GCGC 1 cut(s) 755
AsuC2I CCSGG 2 cut(s) 1260, 1437
AsuHPI GGTGA 4 cut(s) 352, 1425, 1477, 1547
BalI TGGCCA 3 cut(s) 500, 581, 888
BamHI GGATCC 2 cut(s) 1103, 1280
BanII GRGCYC 2 cut(s) 807, 1044
BauI CACGAG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 3 cut(s) 807, 1044, 1428
BbvI GCAGC 3 cut(s) 436, 612, 714
BccI CCATC 5 cut(s) 92, 490, 1000, 1046, 1214
BciT130I CCWGG 1 cut(s) 1454
BciVI GTATCC 1 cut(s) 1450
BcnI CCSGG 2 cut(s) 1260, 1437
BcoDI GTCTC 3 cut(s) 253, 313, 1485
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 4 cut(s) 303, 401, 627, 1169
BfmI CTRYAG 2 cut(s) 422, 857
BfuAI ACCTGC 1 cut(s) 1240
BfuI GTATCC 1 cut(s) 1450
BglII AGATCT 1 cut(s) 1539
BisI GCNGC 5 cut(s) 318, 425, 529, 601, 728
BlsI GCNGC 5 cut(s) 319, 426, 530, 602, 729
BmcAI AGTACT 2 cut(s) 937, 1288
Bme1390I CCNGG 3 cut(s) 1260, 1437, 1454
BmiI GGNNCC 3 cut(s) 1105, 1254, 1282
BmrFI CCNGG 3 cut(s) 1260, 1437, 1454
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 174, 483
BmuI ACTGGG 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 1592
BpuEI CTTGAG 2 cut(s) 237, 1461
BpuMI CCSGG 2 cut(s) 1260, 1437
BsaJI CCNNGG 2 cut(s) 291, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 154, 1277
Bse1I ACTGG 4 cut(s) 195, 999, 1070, 1284
BseBI CCWGG 1 cut(s) 1454
BseDI CCNNGG 2 cut(s) 291, 501
BseGI GGATG 2 cut(s) 501, 1375
BseLI CCNNNNNNNGG 3 cut(s) 153, 154, 1277
BseMII CTCAG 1 cut(s) 1029
BseNI ACTGG 4 cut(s) 195, 999, 1070, 1284
BseRI GAGGAG 3 cut(s) 1150, 1327, 1362
BseXI GCAGC 3 cut(s) 436, 612, 714
BsgI GTGCAG 3 cut(s) 974, 1119, 1296
Bsh1236I CGCG 2 cut(s) 755, 786
BshFI GGCC 5 cut(s) 5, 500, 581, 888, 1056
BsiHKAI GWGCWC 3 cut(s) 807, 1044, 1428
BsiSI CCGG 2 cut(s) 1260, 1437
BslI CCNNNNNNNGG 3 cut(s) 153, 154, 1277
BsmAI GTCTC 3 cut(s) 253, 313, 1485
BsmBI CGTCTC 1 cut(s) 1485
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 5 cut(s) 5, 500, 581, 888, 1056
Bsp1286I GDGCHC 3 cut(s) 807, 1044, 1428
Bsp143I GATC 4 cut(s) 1103, 1280, 1539, 1633
BspACI CCGC 4 cut(s) 318, 528, 755, 786
BspANI GGCC 5 cut(s) 5, 500, 581, 888, 1056
BspCNI CTCAG 1 cut(s) 1030
BspFNI CGCG 2 cut(s) 755, 786
BspLI GGNNCC 3 cut(s) 1105, 1254, 1282
BspMAI CTGCAG 1 cut(s) 426
BspMI ACCTGC 1 cut(s) 1240
BspPI GGATC 4 cut(s) 1098, 1111, 1275, 1288
BspQI GCTCTTC 4 cut(s) 1225, 1239, 1402, 1416
BsrI ACTGG 4 cut(s) 195, 999, 1070, 1284
BssECI CCNNGG 2 cut(s) 291, 501
BssMI GATC 4 cut(s) 1103, 1280, 1539, 1633
BssSI CACGAG 1 cut(s) 713
BssT1I CCWWGG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 1 cut(s) 1454
Bst4CI ACNGT 2 cut(s) 861, 1535
Bst6I CTCTTC 5 cut(s) 1225, 1239, 1402, 1416, 1491
BstC8I GCNNGC 1 cut(s) 1081
BstDEI CTNAG 2 cut(s) 351, 1038
BstF5I GGATG 2 cut(s) 501, 1375
BstFNI CGCG 2 cut(s) 755, 786
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 4 cut(s) 1106, 1283, 1542, 1636
BstMAI GTCTC 3 cut(s) 253, 313, 1485
BstMBI GATC 4 cut(s) 1103, 1280, 1539, 1633
BstNI CCWGG 1 cut(s) 1454
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 3 cut(s) 1258, 1435, 1452
BstSFI CTRYAG 2 cut(s) 422, 857
BstUI CGCG 2 cut(s) 755, 786
BstV1I GCAGC 3 cut(s) 436, 612, 714
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 3 cut(s) 1103, 1280, 1539
BstYI RGATCY 3 cut(s) 1103, 1280, 1539
BsuI GTATCC 1 cut(s) 1450
BsuRI GGCC 5 cut(s) 5, 500, 581, 888, 1056
BtsCI GGATG 2 cut(s) 501, 1375
BtsI GCAGTG 2 cut(s) 203, 459
BtsIMutI CAGTG 3 cut(s) 203, 459, 992
BveI ACCTGC 1 cut(s) 1240
Cac8I GCNNGC 1 cut(s) 1081
CfoI GCGC 1 cut(s) 755
Csp6I GTAC 3 cut(s) 227, 936, 1287
CspCI CAANNNNNGTGG 2 cut(s) 1573, 1608
CviAII CATG 5 cut(s) 42, 461, 553, 897, 1018
CviQI GTAC 3 cut(s) 227, 936, 1287
DdeI CTNAG 2 cut(s) 351, 1038
DpnI GATC 4 cut(s) 1105, 1282, 1541, 1635
DpnII GATC 4 cut(s) 1103, 1280, 1539, 1633
EaeI YGGCCR 4 cut(s) 3, 498, 579, 886
Eam1104I CTCTTC 5 cut(s) 1225, 1239, 1402, 1416, 1491
EarI CTCTTC 5 cut(s) 1225, 1239, 1402, 1416, 1491
Ecl136II GAGCTC 2 cut(s) 805, 1042
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 2 cut(s) 807, 1044
Eco53kI GAGCTC 2 cut(s) 805, 1042
EcoICRI GAGCTC 2 cut(s) 805, 1042
EcoRII CCWGG 1 cut(s) 1452
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 2 cut(s) 807, 1044
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 1485
FaeI CATG 5 cut(s) 45, 464, 556, 900, 1021
FalI AAGNNNNNCTT 6 cut(s) 9, 41, 1207, 1239, 1384, 1416
FatI CATG 5 cut(s) 41, 460, 552, 896, 1017
Fnu4HI GCNGC 5 cut(s) 318, 425, 529, 601, 728
FokI GGATG 2 cut(s) 508, 1362
FriOI GRGCYC 2 cut(s) 807, 1044
Fsp4HI GCNGC 5 cut(s) 318, 425, 529, 601, 728
FspBI CTAG 4 cut(s) 303, 401, 627, 1169
GlaI GCGC 1 cut(s) 754
GluI GCNGC 5 cut(s) 318, 425, 529, 601, 728
GsuI CTGGAG 1 cut(s) 1592
HaeIII GGCC 5 cut(s) 5, 500, 581, 888, 1056
HapII CCGG 2 cut(s) 1260, 1437
HhaI GCGC 1 cut(s) 755
Hin1II CATG 5 cut(s) 45, 464, 556, 900, 1021
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HincII GTYRAC 3 cut(s) 833, 1000, 1627
HindII GTYRAC 3 cut(s) 833, 1000, 1627
HindIII AAGCTT 2 cut(s) 790, 1465
HinfI GANTC 8 cut(s) 296, 417, 556, 663, 772, 781, 1157, 1334
HpaI GTTAAC 2 cut(s) 1000, 1627
HpaII CCGG 2 cut(s) 1260, 1437
HphI GGTGA 4 cut(s) 352, 1425, 1477, 1547
Hpy166II GTNNAC 3 cut(s) 833, 1000, 1627
Hpy188I TCNGA 5 cut(s) 223, 295, 1234, 1272, 1411
Hpy188III TCNNGA 9 cut(s) 613, 700, 736, 874, 1169, 1210, 1293, 1346, 1478
Hpy8I GTNNAC 3 cut(s) 833, 1000, 1627
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 126, 407, 587
HpyCH4III ACNGT 2 cut(s) 861, 1535
HpyCH4IV ACGT 1 cut(s) 1428
HpyCH4V TGCA 9 cut(s) 187, 424, 474, 730, 955, 992, 1079, 1136, 1313
HpyF3I CTNAG 2 cut(s) 351, 1038
HpySE526I ACGT 1 cut(s) 1428
Hsp92II CATG 5 cut(s) 45, 464, 556, 900, 1021
HspAI GCGC 1 cut(s) 753
KspAI GTTAAC 2 cut(s) 1000, 1627
Kzo9I GATC 4 cut(s) 1103, 1280, 1539, 1633
LguI GCTCTTC 4 cut(s) 1225, 1239, 1402, 1416
LmnI GCTCC 2 cut(s) 802, 1551
Lsp1109I GCAGC 3 cut(s) 436, 612, 714
LweI GCATC 2 cut(s) 174, 483
MaeI CTAG 4 cut(s) 303, 401, 627, 1169
MaeII ACGT 1 cut(s) 1428
MaeIII GTNAC 2 cut(s) 358, 973
MalI GATC 4 cut(s) 1105, 1282, 1541, 1635
MboI GATC 4 cut(s) 1103, 1280, 1539, 1633
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 3 cut(s) 1103, 1280, 1539
MhlI GDGCHC 3 cut(s) 807, 1044, 1428
MlsI TGGCCA 3 cut(s) 500, 581, 888
MluNI TGGCCA 3 cut(s) 500, 581, 888
MlyI GAGTC 4 cut(s) 290, 550, 1151, 1328
MmeI TCCRAC 3 cut(s) 201, 640, 1538
Mox20I TGGCCA 3 cut(s) 500, 581, 888
MroXI GAANNNNTTC 3 cut(s) 167, 1219, 1396
MscI TGGCCA 3 cut(s) 500, 581, 888
MslI CAYNNNNRTG 3 cut(s) 40, 1074, 1385
Msp20I TGGCCA 3 cut(s) 500, 581, 888
MspI CCGG 2 cut(s) 1260, 1437
MspR9I CCNGG 3 cut(s) 1260, 1437, 1454
MunI CAATTG 1 cut(s) 838
Mva1269I GAATGC 1 cut(s) 1081
MvaI CCWGG 1 cut(s) 1454
MvnI CGCG 2 cut(s) 755, 786
NciI CCSGG 2 cut(s) 1260, 1437
NdeII GATC 4 cut(s) 1103, 1280, 1539, 1633
NlaIII CATG 5 cut(s) 45, 464, 556, 900, 1021
NlaIV GGNNCC 3 cut(s) 1105, 1254, 1282
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 1385
PciSI GCTCTTC 4 cut(s) 1225, 1239, 1402, 1416
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 3 cut(s) 167, 1219, 1396
PfeI GAWTC 4 cut(s) 417, 663, 772, 781
PkrI GCNGC 5 cut(s) 319, 426, 530, 602, 729
PleI GAGTC 4 cut(s) 290, 550, 1151, 1328
PpsI GAGTC 4 cut(s) 290, 550, 1151, 1328
PshAI GACNNNNGTC 1 cut(s) 99
PshBI ATTAAT 1 cut(s) 671
PsiI TTATAA 1 cut(s) 89
Psp124BI GAGCTC 2 cut(s) 807, 1044
Psp6I CCWGG 1 cut(s) 1452
PspGI CCWGG 1 cut(s) 1452
PspN4I GGNNCC 3 cut(s) 1105, 1254, 1282
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 3 cut(s) 1103, 1280, 1539
RsaI GTAC 3 cut(s) 228, 937, 1288
RsaNI GTAC 3 cut(s) 227, 936, 1287
RseI CAYNNNNRTG 3 cut(s) 40, 1074, 1385
SacI GAGCTC 2 cut(s) 807, 1044
SapI GCTCTTC 4 cut(s) 1225, 1239, 1402, 1416
SatI GCNGC 5 cut(s) 318, 425, 529, 601, 728
Sau3AI GATC 4 cut(s) 1103, 1280, 1539, 1633
ScaI AGTACT 2 cut(s) 937, 1288
SchI GAGTC 4 cut(s) 290, 550, 1151, 1328
ScrFI CCNGG 3 cut(s) 1260, 1437, 1454
SduI GDGCHC 3 cut(s) 807, 1044, 1428
SfaNI GCATC 2 cut(s) 174, 483
SfcI CTRYAG 2 cut(s) 422, 857
SmiMI CAYNNNNRTG 3 cut(s) 40, 1074, 1385
SmlI CTYRAG 2 cut(s) 252, 1476
SmoI CTYRAG 2 cut(s) 252, 1476
SpeI ACTAGT 1 cut(s) 302
SsiI CCGC 4 cut(s) 318, 528, 755, 786
SspMI CTAG 4 cut(s) 303, 401, 627, 1169
SstI GAGCTC 2 cut(s) 807, 1044
StyD4I CCNGG 3 cut(s) 1258, 1435, 1452
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 861, 1535
TaiI ACGT 1 cut(s) 1431
TaqI TCGA 6 cut(s) 93, 236, 636, 907, 1094, 1632
TaqII GACCGA 1 cut(s) 433
TatI WGTACW 2 cut(s) 935, 1286
TauI GCSGC 2 cut(s) 320, 531
TfiI GAWTC 4 cut(s) 417, 663, 772, 781
TscAI CASTG 3 cut(s) 210, 459, 999
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 424, 600, 727
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 7 cut(s) 95, 449, 562, 839, 885, 1191, 1368
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 3 cut(s) 210, 459, 999
VspI ATTAAT 1 cut(s) 671
XapI RAATTY 2 cut(s) 1129, 1306
XbaI TCTAGA 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 2 cut(s) 881, 1010
XmnI GAANNNNTTC 3 cut(s) 167, 1219, 1396
XspI CTAG 4 cut(s) 303, 401, 627, 1169
ZrmI AGTACT 2 cut(s) 937, 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.