RchiOBHm_Chr2g0099201

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004264
Physical Location & Seq
Reverse (-)
11477018 .. 11484605
7588 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3018 bp
ATGCTAAAGCTCGCGGCGTTTTTCGAGCTTGTGATTGTGGTTGTTTGGTTGGGAGTGGCGAGTGTTGATGGAGTTTTTGTGAAGTACCAGACCGGGAGTGCTGTTGTGGAAGGGAAGTTGAATGTTCATTTGGTTGCGCATTCCCATGATGACGTTGGTTGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAATAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTCGTTGAATCGTTGGTTCGTGATCCGAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTTACACAAAGTGTGGAAGTTCAAGAACGAGTGCGGAAACTAGTAGATGCTGGCCAGTTAGAGTTTGTAAATGGAGGTTGGTGCATGCATGATGAGGCTGCAGTCCATTACATAGACATGATTGACCAAACAACTCTAGGCCACCTTGCTATAAAGCAGCAGTTCAACAAGACACCTCGAGCGGGGTGGCAAATTGATCCGTTCGGACACTCTGCTGTGCAGGGTTACTTACTTGGAGCTGAGCTTGGATTCGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGCACAGCGTAAGGGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTCGTCACTATAGTCCGCCAGATGGTTTCCATTTTGAAGTTTTTGATGACTTTGTCCCTGTGCAGGATAATCCGCTTATTTTTGACTACAATGTTGAACTGCGCGTTAATGATTTTATTACTGCTGCAATCATCCAAGCAAATGTGACTAGGGGAAATCACATGATGTGGACAATGGGAGATGATTTCCAGTACCAATATGCTGAATCTTGGTTCAAACAAATGGATAAGTTAATTCACTATGTTAATAAGGATGGCCGAGTTAATGCCTTATATTCTACACCGTCTCTCTACACTGATGCTAAAAATGCAGCAAATCAATCATGGCCATTAAAAACTGAGGATTATTTTCCGTATGCAGATATAATAAATGCTTATTGGACCGGTTATTTTACTAGTCGCCCAGGTTTGAAAAGATATATTCGATCACTAAGCGGATATTATTTGGCAGCAAGAGAACTAGAATTTCTGGTTGGAAAGAGAGCAAATGGTTCAAGTACCTATTCCCTTGGAGATGCTTTAGGAATTGCACAGCACCACGATGCTGCCACTGGCACTGCTAAACAACATACAACAAATGACTATGTAAAGCGCCTGTCAATTGGTGTCACTGAGGCAGAAGCTGTTGTCAGTTCTGCTCTGTCGTGCCTAACTACAAATAAATCTGGAGATCAATGTAAAGACCCTGCAGTTACTTTTAGCCAGTGTCGACTACTCAATATCAGTTTCTGCCCTCCAACGGAGGAAGATATTCCAGAAGGGAAGAGCTTGGTTGTTGTGGCATATAATCCTCTTGGATGGAATCGCACTGAGATTGTTAGGATACCTGTTAATTATGATGATCTTGTTGTTCAAGATTCCTCCGGAAACATCCTCGAGACACAATATGTGAGTGTGGATAATGTTACAAGCAATCTAAGAACATTTTATACAGAAGCCTACTTGGGACAGTCATCAAAACAAATTCCCAAGTACTGGCTAATCTTTCAAGCCTCTGTGTCACCACTTGGTTGGGACACATACTTCATCTCTAGAGGAGCTACCAAAGGGAAAAGAAGAGTTGGACTTCTCTCTGTGAAGGACAGTCCACAGAATGAGACCATTGAAATTGGACCAGGAGATCTGAAGATGTCCTTATCTTCTGCTTCTGGACAACTCAAGCGGATGTATAATTCTAAAACAGGAGTTGATGTACCAATACAGCAGAGCTACCTCTGGTATGGTTCAAGCACCGATGGCCAGGCTTCAGGTGCATATATATTCCGGCCTAATGGTGCCCCTCCAACTATTGTGTCAAGATCTGTACCCTTGAAGGTCTTTCGTGGACCATTATTCGATGAGGTTCACCAAGAATTCAGTTCATGGATCTATCAGGTCATTAGAGTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACTATTGGTCCAATTCCTACAGACGACGGTGTTGGTAAAGAGGTCATCACACGAATGACAGCAAACATGGAGACTAACAAGATGTTTTACACCGATTCCAATGGGAGGGATTTCATTAAAAGGGTACGAGATTATAGAGCAGACTGGAACCTTTCAGTTAATCAACCTGTTGCAGGAAACTACTATCCACTAAACCTTGGGATCTTTACTTCGGATAACAAATCTGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCCAGCATTCAAGACGGACAAGTAGAGATGATGCTACATAGGCGTCTACTGGTCGATGATGGTAGAGGGGTGGGTGAAGCCCTTGATGAAACAGTGTGCATCAAGAATAATACATGTCAAGGACTAACGGTTCGAGGAAACTATTATATGAGCATAAACCAGTTCGGGGATGGTGCCCAATGGCGTCGGACAACTGGACAAGAAGTTTACTCGCCACTTCTTCTAGCTTTCACCCATGAGAACTTGGAGAATTGGACTGCATCTCATTTAACAAAAGGGTCTGTCATGGATTCAAGTTACAGCTTGCCTCCCAATGTTGCTTTGATTACTCTTCAGGAGTTGGATGGTGGAAGTGTACTTCTCCGTCTAGCACATCTATATGAGGCTGCTGAAGCTCCTCAATATTCTACATTGGCAAAAGTTGAACTGAAGAAGATGCTAACAGGAAAAACGATAAAGCAGTTGAAAGAAGTGAGCTTGTCAGCAAACCAAGAGAAGTCAGCGATGAAGAAGATGACGTGGAAAGTCGAGGGTTCCACAGGAGAAGAACCTACACCAGTAAGAGGCGGCCCTGTCGATAGTTCAACTCTTGTTGTTGAGCTTGGACCCATGGAGATACGTACTTTCTTATTGAAATTTTAG

Protein Analysis

1005

Amino Acids

112.58

Weight (kDa)

5.85

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 41 - 352 6.1e-77 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 357 - 449 5e-18 Alpha mannosidase middle domain
Laman-like_dom PF21260 539 - 575 9.2e-06 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 605 - 814 3.1e-45 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 897 - 1001 5.2e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 1970, 2549
AccB7I CCANNNNNTGG 1 cut(s) 1671
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 2 cut(s) 1405, 2422
AccII CGCG 2 cut(s) 14, 801
AccIII TCCGGA 1 cut(s) 1559
AciI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
AclWI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AcoI YGGCCR 4 cut(s) 367, 952, 1022, 1933
AcsI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
AcuI CTGAAG 5 cut(s) 1841, 1926, 2693, 2787, 2825
AcyI GRCGYC 2 cut(s) 2419, 2560
AdeI CACNNNGTG 1 cut(s) 326
AfiI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2542, 2939
AflIII ACRYGT 1 cut(s) 2489
AgeI ACCGGT 1 cut(s) 1079
AhlI ACTAGT 2 cut(s) 355, 1091
AjiI CACGTC 1 cut(s) 2895
AjnI CCWGG 3 cut(s) 1099, 1810, 1935
AjuI GAANNNNNNNTTGG 6 cut(s) 113, 145, 1152, 1184, 2103, 2135
AloI GAACNNNNNNTCC 2 cut(s) 2490, 2522
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
AlwI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AlwNI CAGNNNCTG 2 cut(s) 1319, 1425
Ama87I CYCGRG 2 cut(s) 492, 1571
Aor13HI TCCGGA 1 cut(s) 1559
AoxI GGCC 7 cut(s) 367, 454, 952, 1022, 1933, 1961, 2944
ApeKI GCWGC 7 cut(s) 413, 472, 821, 1007, 1145, 1241, 2762
ApoI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
ArsI GACNNNNNNTTYG 2 cut(s) 2490, 2522
AsiGI ACCGGT 1 cut(s) 1079
Asp700I GAANNNNTTC 1 cut(s) 1446
AspLEI GCGC 3 cut(s) 139, 801, 1290
AspS9I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 4 cut(s) 1689, 2033, 2462, 2599
AvaI CYCGRG 2 cut(s) 492, 1571
AvaII GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
BaeGI GKGCMC 2 cut(s) 1975, 2554
BalI TGGCCA 3 cut(s) 369, 1024, 1935
BamHI GGATCC 2 cut(s) 650, 2375
BanI GGYRCC 2 cut(s) 1970, 2549
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 7 cut(s) 400, 484, 808, 1019, 1157, 1228, 2749
BciT130I CCWGG 3 cut(s) 1101, 1812, 1937
BciVI GTATCC 1 cut(s) 1512
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BcuI ACTAGT 2 cut(s) 355, 1091
BfaI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2600, 2744
BfmI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BfoI RGCGCY 1 cut(s) 1291
BfuI GTATCC 1 cut(s) 1512
BglII AGATCT 2 cut(s) 1816, 1994
BisI GCNGC 9 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2763, 2944
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 9 cut(s) 16, 415, 474, 823, 1009, 1147, 1243, 2764, 2945
BmcAI AGTACT 2 cut(s) 182, 1670
Bme1390I CCNGG 4 cut(s) 94, 1101, 1812, 1937
Bme18I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
BmeT110I CYCGRG 2 cut(s) 492, 1571
BmgBI CACGTC 1 cut(s) 2895
BmgT120I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
BmiI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2551, 2911, 2983
BmrFI CCNGG 4 cut(s) 94, 1101, 1812, 1937
BmsI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 1383, 2391
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 1838
BpuMI CCSGG 1 cut(s) 94
BsaAI YACGTR 1 cut(s) 2996
BsaHI GRCGYC 2 cut(s) 2419, 2560
BsaI GGTCTC 1 cut(s) 1787
BsaJI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BsaWI WCCGGW 2 cut(s) 1079, 1559
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1872, 1902
Bsc4I CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2542, 2939
Bse118I RCCGGY 1 cut(s) 1079
BseAI TCCGGA 1 cut(s) 1559
BseBI CCWGG 3 cut(s) 1101, 1812, 1937
BseDI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BseGI GGATG 7 cut(s) 828, 955, 1499, 1566, 1866, 2551, 2725
BseLI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2542, 2939
BseMII CTCAG 8 cut(s) 279, 546, 689, 1026, 1299, 1497, 2331, 2361
BseRI GAGGAG 2 cut(s) 1746, 2763
BseSI GKGCMC 2 cut(s) 1975, 2554
BseXI GCAGC 7 cut(s) 400, 484, 808, 1019, 1157, 1228, 2749
BsgI GTGCAG 2 cut(s) 554, 779
Bsh1236I CGCG 2 cut(s) 14, 801
BshFI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BshNI GGYRCC 2 cut(s) 1970, 2549
BshTI ACCGGT 1 cut(s) 1079
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 2 cut(s) 492, 1571
BsiSI CCGG 4 cut(s) 93, 1080, 1560, 1960
BslFI GGGAC 3 cut(s) 737, 1656, 1724
BslI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2542, 2939
BsmAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 3 cut(s) 737, 1656, 1724
BsmI GAATGC 3 cut(s) 139, 204, 2382
BsnI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
Bso31I GGTCTC 1 cut(s) 1787
BsoBI CYCGRG 2 cut(s) 492, 1571
Bsp1286I GDGCHC 3 cut(s) 610, 1975, 2554
Bsp13I TCCGGA 1 cut(s) 1559
Bsp1407I TGTACA 1 cut(s) 574
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 2985
BspACI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
BspANI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BspCNI CTCAG 8 cut(s) 280, 547, 688, 1027, 1300, 1498, 2332, 2360
BspEI TCCGGA 1 cut(s) 1559
BspFNI CGCG 2 cut(s) 14, 801
BspLI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2551, 2911, 2983
BspMAI CTGCAG 2 cut(s) 418, 1387
BspPI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 2 cut(s) 1970, 2549
BspTNI GGTCTC 1 cut(s) 1787
BsrBI CCGCTC 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 4 cut(s) 1099, 1204, 2311, 2985
BssNI GRCGYC 2 cut(s) 2419, 2560
BssT1I CCWWGG 3 cut(s) 1204, 2311, 2985
Bst2UI CCWGG 3 cut(s) 1101, 1812, 1937
Bst4CI ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
Bst6I CTCTTC 3 cut(s) 1454, 1747, 2712
BstACI GRCGYC 2 cut(s) 2419, 2560
BstAUI TGTACA 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 2996
BstC8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2681
BstDSI CCRYGG 1 cut(s) 2985
BstENI CCTNNNNNAGG 1 cut(s) 2286
BstF5I GGATG 7 cut(s) 828, 955, 1499, 1566, 1866, 2551, 2725
BstFNI CGCG 2 cut(s) 14, 801
BstH2I RGCGCY 1 cut(s) 1291
BstHHI GCGC 3 cut(s) 139, 801, 1290
BstMAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BstMWI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
BstNI CCWGG 3 cut(s) 1101, 1812, 1937
BstNSI RCATGY 3 cut(s) 403, 2102, 2493
BstSCI CCNGG 4 cut(s) 92, 1099, 1810, 1935
BstSFI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BstSLI GKGCMC 2 cut(s) 1975, 2554
BstSNI TACGTA 1 cut(s) 2996
BstUI CGCG 2 cut(s) 14, 801
BstV1I GCAGC 7 cut(s) 400, 484, 808, 1019, 1157, 1228, 2749
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BstXI CCANNNNNNTGG 1 cut(s) 1707
BstYI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BsuI GTATCC 1 cut(s) 1512
BsuRI GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
BtgI CCRYGG 1 cut(s) 2985
BtgZI GCGATG 1 cut(s) 2894
BtrI CACGTC 1 cut(s) 2895
BtsCI GGATG 7 cut(s) 828, 955, 1499, 1566, 1866, 2551, 2725
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 8 cut(s) 990, 1245, 1251, 1305, 1406, 1503, 2367, 2475
Cac8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2681
CaiI CAGNNNCTG 2 cut(s) 1319, 1425
CfoI GCGC 3 cut(s) 139, 801, 1290
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
CseI GACGC 2 cut(s) 2408, 2549
CspAI ACCGGT 1 cut(s) 1079
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
DraIII CACNNNGTG 1 cut(s) 326
EaeI YGGCCR 4 cut(s) 367, 952, 1022, 1933
Eam1104I CTCTTC 3 cut(s) 1454, 1747, 2712
EarI CTCTTC 3 cut(s) 1454, 1747, 2712
EciI GGCGGA 1 cut(s) 702
Eco105I TACGTA 1 cut(s) 2996
Eco130I CCWWGG 3 cut(s) 1204, 2311, 2985
Eco31I GGTCTC 1 cut(s) 1787
Eco47I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
Eco57I CTGAAG 5 cut(s) 1841, 1926, 2693, 2787, 2825
Eco88I CYCGRG 2 cut(s) 492, 1571
EcoNI CCTNNNNNAGG 1 cut(s) 2286
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 3 cut(s) 1099, 1810, 1935
EcoT14I CCWWGG 3 cut(s) 1204, 2311, 2985
EcoT22I ATGCAT 1 cut(s) 405
ErhI CCWWGG 3 cut(s) 1204, 2311, 2985
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 1814, 1846
FaqI GGGAC 3 cut(s) 737, 1656, 1724
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 2 cut(s) 1405, 2422
Fnu4HI GCNGC 9 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2763, 2944
FokI GGATG 7 cut(s) 815, 962, 1506, 1553, 1873, 2558, 2732
Fsp4HI GCNGC 9 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2763, 2944
FspBI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2600, 2744
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 3 cut(s) 138, 800, 1289
GluI GCNGC 9 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2763, 2944
GsuI CTGGAG 2 cut(s) 1383, 2391
HaeII RGCGCY 1 cut(s) 1291
HaeIII GGCC 7 cut(s) 369, 456, 954, 1024, 1935, 1963, 2946
HapII CCGG 4 cut(s) 93, 1080, 1560, 1960
HgaI GACGC 2 cut(s) 2408, 2549
HhaI GCGC 3 cut(s) 139, 801, 1290
Hin1I GRCGYC 2 cut(s) 2419, 2560
Hin6I GCGC 3 cut(s) 137, 799, 1288
HinP1I GCGC 3 cut(s) 137, 799, 1288
HincII GTYRAC 1 cut(s) 1406
HindII GTYRAC 1 cut(s) 1406
HinfI GANTC 9 cut(s) 236, 248, 564, 569, 902, 1498, 1553, 2210, 2666
HpaII CCGG 4 cut(s) 93, 1080, 1560, 1960
HphI GGTGA 4 cut(s) 1689, 2033, 2462, 2599
Hpy188I TCNGA 7 cut(s) 267, 521, 678, 1821, 2329, 2341, 2565
Hpy99I CGWCG 2 cut(s) 2144, 2565
HpyAV CCTTC 4 cut(s) 104, 1448, 1768, 2002
HpyCH4III ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
HpyCH4IV ACGT 4 cut(s) 153, 230, 2894, 2995
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
HpySE526I ACGT 4 cut(s) 153, 230, 2894, 2995
Hsp92I GRCGYC 2 cut(s) 2419, 2560
HspAI GCGC 3 cut(s) 137, 799, 1288
Kpn2I TCCGGA 1 cut(s) 1559
Ksp22I TGATCA 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 3 cut(s) 551, 1733, 2776
Lsp1109I GCAGC 7 cut(s) 400, 484, 808, 1019, 1157, 1228, 2749
LweI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
MaeI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2600, 2744
MaeII ACGT 4 cut(s) 153, 230, 2894, 2995
MaeIII GTNAC 8 cut(s) 539, 701, 841, 1303, 1387, 1600, 1695, 2672
MbiI CCGCTC 1 cut(s) 497
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
MhlI GDGCHC 3 cut(s) 610, 1975, 2554
MlsI TGGCCA 3 cut(s) 369, 1024, 1935
MluNI TGGCCA 3 cut(s) 369, 1024, 1935
MmeI TCCRAC 8 cut(s) 169, 213, 1150, 1457, 1738, 2003, 2543, 2697
Mox20I TGGCCA 3 cut(s) 369, 1024, 1935
Mph1103I ATGCAT 1 cut(s) 405
MroI TCCGGA 1 cut(s) 1559
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 3 cut(s) 369, 1024, 1935
MseI TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2645
MslI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
Msp20I TGGCCA 3 cut(s) 369, 1024, 1935
MspI CCGG 4 cut(s) 93, 1080, 1560, 1960
MspR9I CCNGG 4 cut(s) 94, 1101, 1812, 1937
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 3 cut(s) 139, 204, 2382
MvaI CCWGG 3 cut(s) 1101, 1812, 1937
MvnI CGCG 2 cut(s) 14, 801
MwoI GCNNNNNNNGC 6 cut(s) 293, 1004, 1932, 1946, 2416, 2768
NciI CCSGG 1 cut(s) 94
NcoI CCATGG 1 cut(s) 2985
NlaIV GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2551, 2911, 2983
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 4 cut(s) 701, 841, 1303, 1695
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 403, 2102, 2493
PaeI GCATGC 2 cut(s) 403, 2102
PaeR7I CTCGAG 2 cut(s) 492, 1571
PciI ACATGT 1 cut(s) 2489
PciSI GCTCTTC 1 cut(s) 1454
PctI GAATGC 3 cut(s) 139, 204, 2382
PdmI GAANNNNTTC 1 cut(s) 1446
PfeI GAWTC 9 cut(s) 236, 248, 564, 569, 902, 1498, 1553, 2210, 2666
PflFI GACNNNGTC 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 1671
PinAI ACCGGT 1 cut(s) 1079
PkrI GCNGC 9 cut(s) 16, 415, 474, 823, 1009, 1147, 1243, 2764, 2945
Ppu21I YACGTR 1 cut(s) 2996
PscI ACATGT 1 cut(s) 2489
Psp6I CCWGG 3 cut(s) 1099, 1810, 1935
PspGI CCWGG 3 cut(s) 1099, 1810, 1935
PspN4I GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2551, 2911, 2983
PspPI GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
PspXI VCTCGAGB 1 cut(s) 492
PsrI GAACNNNNNNTAC 2 cut(s) 1609, 1641
PstI CTGCAG 2 cut(s) 418, 1387
PstNI CAGNNNCTG 2 cut(s) 1319, 1425
PsuI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
PsyI GACNNNGTC 1 cut(s) 749
RseI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 1 cut(s) 1454
SaqAI TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2645
SatI GCNGC 9 cut(s) 15, 414, 473, 822, 1008, 1146, 1242, 2763, 2944
Sau96I GGNCC 6 cut(s) 1077, 1808, 2021, 2123, 2945, 2981
ScaI AGTACT 2 cut(s) 182, 1670
ScrFI CCNGG 4 cut(s) 94, 1101, 1812, 1937
SduI GDGCHC 3 cut(s) 610, 1975, 2554
SfaNI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2484, 2645, 2802
SfcI CTRYAG 4 cut(s) 414, 706, 1383, 2133
Sfr274I CTCGAG 2 cut(s) 492, 1571
SinI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
SlaI CTCGAG 2 cut(s) 492, 1571
SmiMI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2754
SmlI CTYRAG 3 cut(s) 492, 1571, 1853
SmoI CTYRAG 3 cut(s) 492, 1571, 1853
SnaBI TACGTA 1 cut(s) 2996
SpeI ACTAGT 2 cut(s) 355, 1091
SphI GCATGC 2 cut(s) 403, 2102
SsiI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2943
SspI AATATT 1 cut(s) 2780
SspMI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2600, 2744
StyD4I CCNGG 4 cut(s) 92, 1099, 1810, 1935
StyI CCWWGG 3 cut(s) 1204, 2311, 2985
TaaI ACNGT 7 cut(s) 172, 981, 1647, 1781, 2144, 2470, 2506
TaiI ACGT 4 cut(s) 156, 233, 2897, 2998
TatI WGTACW 4 cut(s) 180, 574, 1668, 2731
TauI GCSGC 2 cut(s) 17, 2946
TfiI GAWTC 9 cut(s) 236, 248, 564, 569, 902, 1498, 1553, 2210, 2666
Tru1I TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2645
Tru9I TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2645
TscAI CASTG 8 cut(s) 997, 1252, 1258, 1312, 1406, 1510, 2374, 2475
TseFI GTSAC 4 cut(s) 701, 841, 1303, 1695
TseI GCWGC 7 cut(s) 413, 472, 821, 1007, 1145, 1241, 2762
Tsp45I GTSAC 4 cut(s) 701, 841, 1303, 1695
TspDTI ATGAA 7 cut(s) 116, 660, 1711, 2046, 2218, 2478, 2897
TspGWI ACGGA 5 cut(s) 504, 1038, 1451, 2406, 2729
TspRI CASTG 8 cut(s) 997, 1252, 1258, 1312, 1406, 1510, 2374, 2475
Tth111I GACNNNGTC 1 cut(s) 749
Van91I CCANNNNNTGG 1 cut(s) 1671
VpaK11BI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2981
XagI CCTNNNNNAGG 1 cut(s) 2286
XapI RAATTY 4 cut(s) 1160, 1659, 2048, 3011
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 3 cut(s) 403, 2102, 2493
XcmI CCANNNNNNNNNTGG 2 cut(s) 152, 2543
XhoI CTCGAG 2 cut(s) 492, 1571
XmiI GTMKAC 2 cut(s) 1405, 2422
XmnI GAANNNNTTC 1 cut(s) 1446
XspI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2600, 2744
ZrmI AGTACT 2 cut(s) 182, 1670
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.