RchiOBHm_Chr2g0106331

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005695
Physical Location & Seq
Forward (+)
17760588 .. 17762245
1658 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGGCCACTGACTTCAAGTCCATACCCATAATTGATATTGGTCCTCTTCTGGCCAAGTGTGATGATCCCAAAATGGGTCAAGACCCAGGTGTTGCTCATGTAGTCAAGCAATTAGATCAAGCTTGTAAAGAAGCAGGCTTCTTCTATGTGAAAGGCCATGGTGTTCCTGAGACTCTGTTAAAAGAAGTCAAGAATCTGACCCGCAAGTTCTTTGAACTTCCATATGAGGAAAAAGCCAAGATCAAGATGACTGCTGATAAAGGATACAGAGGGTATCAGAGAATTGGGGAGAATATAACCAAAGGTGTACCAGACATACATGAAGCAATTGATTGCTATAAAGAAGTGAAACCAGGAATGTACGGAGATCTTGGAAAACCTATGGAAGGATGTAACCAATGGCCAACTAATCCCCCAAACTTCAAGCTACTGATGGAGGAATATATCAGCCTCTGCACAGGCATGCTTTCCTCAACAAACCAACTTCTCACTGTTTATCATTCCATGTTCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

171

Amino Acids

19.21

Weight (kDa)

6.42

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 8 - 137 1.7e-32 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 202
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 3 cut(s) 3, 51, 401
AfaI GTAC 2 cut(s) 309, 362
AfiI CCNNNNNNNGG 2 cut(s) 74, 386
AgsI TTSAA 3 cut(s) 16, 215, 424
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 2 cut(s) 85, 352
AluBI AGCT 2 cut(s) 122, 427
AluI AGCT 2 cut(s) 122, 427
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 1 cut(s) 453
AoxI GGCC 4 cut(s) 3, 51, 154, 401
AspS9I GGNCC 1 cut(s) 41
AvaII GGWCC 1 cut(s) 41
BalI TGGCCA 3 cut(s) 5, 53, 403
BccI CCATC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 87, 354
BciVI GTATCC 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 514
BfuI GTATCC 1 cut(s) 257
BglII AGATCT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 87, 354
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 41
BmrFI CCNGG 2 cut(s) 87, 354
BsaJI CCNNGG 2 cut(s) 85, 157
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 386
BseBI CCWGG 2 cut(s) 87, 354
BseDI CCNNGG 2 cut(s) 85, 157
BseGI GGATG 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 74, 386
BseMII CTCAG 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 439
BshFI GGCC 4 cut(s) 5, 53, 156, 403
BslI CCNNNNNNNGG 2 cut(s) 74, 386
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 4 cut(s) 5, 53, 156, 403
Bsp143I GATC 4 cut(s) 64, 115, 240, 367
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 1 cut(s) 202
BspANI GGCC 4 cut(s) 5, 53, 156, 403
BspCNI CTCAG 1 cut(s) 160
BspPI GGATC 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 85, 157
BssMI GATC 4 cut(s) 64, 115, 240, 367
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 87, 354
Bst4CI ACNGT 1 cut(s) 493
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 136, 464
BstDEI CTNAG 1 cut(s) 168
BstDSI CCRYGG 1 cut(s) 157
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 395
BstKTI GATC 4 cut(s) 67, 118, 243, 370
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 4 cut(s) 64, 115, 240, 367
BstNI CCWGG 2 cut(s) 87, 354
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 85, 352
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 4 cut(s) 5, 53, 156, 403
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 395
BtsIMutI CAGTG 2 cut(s) 6, 489
Cac8I GCNNGC 2 cut(s) 136, 464
CaiI CAGNNNCTG 1 cut(s) 453
Cfr13I GGNCC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 308, 361
CviAII CATG 5 cut(s) 98, 158, 320, 463, 505
CviJI RGCY 9 cut(s) 5, 53, 122, 138, 156, 236, 403, 427, 450
CviKI_1 RGCY 9 cut(s) 5, 53, 122, 138, 156, 236, 403, 427, 450
CviQI GTAC 2 cut(s) 308, 361
DdeI CTNAG 1 cut(s) 168
DpnI GATC 4 cut(s) 66, 117, 242, 369
DpnII GATC 4 cut(s) 64, 115, 240, 367
DriI GACNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 3 cut(s) 3, 51, 401
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 41
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoRII CCWGG 2 cut(s) 85, 352
EcoT14I CCWWGG 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 5 cut(s) 101, 161, 323, 466, 508
FatI CATG 5 cut(s) 97, 157, 319, 462, 504
FauI CCCGC 1 cut(s) 209
FauNDI CATATG 1 cut(s) 223
FokI GGATG 1 cut(s) 402
FspBI CTAG 1 cut(s) 514
HaeIII GGCC 4 cut(s) 5, 53, 156, 403
Hin1II CATG 5 cut(s) 101, 161, 323, 466, 508
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 2 cut(s) 172, 193
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 2 cut(s) 198, 279
Hpy188III TCNNGA 4 cut(s) 80, 167, 190, 244
Hpy8I GTNNAC 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4V TGCA 1 cut(s) 456
HpyF3I CTNAG 1 cut(s) 168
Hsp92II CATG 5 cut(s) 101, 161, 323, 466, 508
Kzo9I GATC 4 cut(s) 64, 115, 240, 367
LpnPI CCDG 9 cut(s) 35, 72, 99, 120, 180, 324, 339, 366, 444
MaeI CTAG 1 cut(s) 514
MaeIII GTNAC 1 cut(s) 392
MalI GATC 4 cut(s) 66, 117, 242, 369
MboI GATC 4 cut(s) 64, 115, 240, 367
MboII GAAGA 2 cut(s) 38, 133
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 1 cut(s) 367
MlsI TGGCCA 3 cut(s) 5, 53, 403
MluCI AATT 4 cut(s) 30, 110, 282, 327
MluNI TGGCCA 3 cut(s) 5, 53, 403
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 6 cut(s) 54, 220, 263, 430, 461, 481
Mox20I TGGCCA 3 cut(s) 5, 53, 403
MscI TGGCCA 3 cut(s) 5, 53, 403
MseI TTAA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 461
Msp20I TGGCCA 3 cut(s) 5, 53, 403
MspR9I CCNGG 2 cut(s) 87, 354
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 2 cut(s) 87, 354
NcoI CCATGG 1 cut(s) 157
NdeI CATATG 1 cut(s) 223
NdeII GATC 4 cut(s) 64, 115, 240, 367
NlaIII CATG 5 cut(s) 101, 161, 323, 466, 508
NspI RCATGY 1 cut(s) 466
PaeI GCATGC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 193
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
Psp6I CCWGG 2 cut(s) 85, 352
PspGI CCWGG 2 cut(s) 85, 352
PspPI GGNCC 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 453
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 309, 362
RsaNI GTAC 2 cut(s) 308, 361
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 1 cut(s) 179
Sau3AI GATC 4 cut(s) 64, 115, 240, 367
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 87, 354
SetI ASST 5 cut(s) 91, 124, 307, 382, 429
SinI GGWCC 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 461
SphI GCATGC 1 cut(s) 466
Sse9I AATT 4 cut(s) 30, 110, 282, 327
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 514
StyD4I CCNGG 2 cut(s) 85, 352
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 493
TasI AATT 4 cut(s) 30, 110, 282, 327
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TscAI CASTG 2 cut(s) 13, 496
TspDTI ATGAA 1 cut(s) 336
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 2 cut(s) 13, 496
VpaK11BI GGWCC 1 cut(s) 41
XagI CCTNNNNNAGG 1 cut(s) 384
XceI RCATGY 1 cut(s) 466
XspI CTAG 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.