RchiOBHm_Chr1g0313091
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006438
Physical Location & Seq
Forward (+)
173562 .. 175299
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1179 bp
ATGGATGATTTCGACTTAGAACTGGATGAGATGGAATTAACTGCAGCAGCGGCTGGGTACCATTATTATAGCAGTATTACCAAGCAACCTTGTCATAGTTTGTCACCAAGTAAAGGATGTGGATTTATGACAGAGGTGCTAAATGGTCATGATGTTGTGTTCCAGGAAATGTTTCGGATGGATAAAAATGTATTTCACAAATTGTGTGACATTCTTCGGCGCAGAGGCATGTTGCGTGATACTGCTGGTGTTATGATAGAGGAGCAGCTTGGAATATTTTTGAATATTATTGGCCATAATGAGCGGAATAGAGTAATACAAGAGCGGTTTCATCACTCAGGTGAAACCATCAGCCGTCATTTCAACAATGTGTTGAAGGCTGTCAAGTCGCTTTCACGTGAATTTTTACAAACTCCAAGCCCCAGCACTCCTCCAGAAATCCTTGGAAATAACAGATTTTATCCTTATTTCCAGGATTGTATCGGAGTCATTGGTGGAATGCACATTCCTGCCCAGGTGCCAGCCAAAGATCAATCCCGCTTTCGTAATAAGAAAGGTGTTCTTTCACAAAATGTGTTGGCAGCTTGCACGTTTGATCTTCAGTTTATATTCGTTTATCCTGGTTGGGAAGGCTCTGCTGCAGGTTCACGTGTATTGAGAGCAGTCCTTGACGATCCACATCAGAATTTCCCACATATTCCTGAAGGGAAATATTTCCTTGTTGACACTGGATATGCAAATATGAAAGGATTTATTGCGCCATTCCAAGGGGTCAGGTATCATGTCCATGAGTATAGAGGTGCTAATCAGTTACCTAGAAATGCAAGGGAGCTCTTTAACCACAGACACTCGTCTCTTAGTAATGTCATCCAGAGGTCTTTCCATGTGTTGAAAACTAGGTTTCCTATTCTCAAACTTGCTCCTCAGTATGCTTTTCATACTCAAAGAGATATAGTTATAGCTGCTTGCGTTCTACATAATTACATCCGACGTGAGTTGAAAAATGATTGGCTGTTTTCCAGCGTGGAAGGGTCAACAGTGGATGACCTACCTGATGTTGATGAACATCCTGATATTGGGTTGGTTTCTTCGGTACAAGAGCAGGTTGCGTTTTCAATGAGAGAATCAATTTCAGCAGAAATGTGGGATCACTTTATGAATGGATGGGATCAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

392

Amino Acids

44.97

Weight (kDa)

6.46

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 37 - 129 1.5e-36 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 165 - 327 3.5e-21 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 632, 1093
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 2 cut(s) 57, 517
AccBSI CCGCTC 2 cut(s) 304, 325
AciI CCGC 4 cut(s) 50, 304, 325, 538
AclWI GGATC 3 cut(s) 668, 1155, 1176
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 2 cut(s) 401, 685
AcuI CTGAAG 2 cut(s) 584, 723
AcvI CACGTG 2 cut(s) 398, 650
AfaI GTAC 2 cut(s) 59, 1095
AfiI CCNNNNNNNGG 3 cut(s) 113, 767, 1076
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 6 cut(s) 283, 364, 376, 892, 1000, 1116
AjiI CACGTC 1 cut(s) 992
AjnI CCWGG 4 cut(s) 162, 471, 513, 619
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 4 cut(s) 268, 584, 832, 962
AluI AGCT 4 cut(s) 268, 584, 832, 962
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 1 cut(s) 858
AlwI GGATC 3 cut(s) 668, 1155, 1176
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 6 cut(s) 44, 47, 265, 581, 638, 962
ApoI RAATTY 2 cut(s) 401, 685
Asp700I GAANNNNTTC 2 cut(s) 171, 713
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 2 cut(s) 222, 760
AsuHPI GGTGA 2 cut(s) 96, 353
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 2 cut(s) 57, 517
BanII GRGCYC 1 cut(s) 834
BbrPI CACGTG 2 cut(s) 398, 650
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 6 cut(s) 56, 59, 277, 593, 625, 949
BccI CCATC 4 cut(s) 25, 172, 356, 1158
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 4 cut(s) 164, 473, 515, 621
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 2 cut(s) 816, 897
BfmI CTRYAG 2 cut(s) 42, 639
BfuAI ACCTGC 2 cut(s) 632, 1093
BisI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
BlsI GCNGC 7 cut(s) 46, 49, 52, 267, 583, 640, 964
Bme1390I CCNGG 4 cut(s) 164, 473, 515, 621
BmgBI CACGTC 1 cut(s) 992
BmiI GGNNCC 2 cut(s) 59, 519
BmrFI CCNGG 4 cut(s) 164, 473, 515, 621
BoxI GACNNNNGTC 1 cut(s) 850
BpmI CTGGAG 1 cut(s) 417
BsaAI YACGTR 2 cut(s) 398, 650
BsaBI GATNNNNATC 2 cut(s) 678, 1065
BsaJI CCNNGG 3 cut(s) 442, 513, 766
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 767, 1076
Bse1I ACTGG 2 cut(s) 27, 733
Bse8I GATNNNNATC 2 cut(s) 678, 1065
BseBI CCWGG 4 cut(s) 164, 473, 515, 621
BseDI CCNNGG 3 cut(s) 442, 513, 766
BseGI GGATG 9 cut(s) 10, 31, 122, 183, 867, 984, 1048, 1066, 1169
BseJI GATNNNNATC 2 cut(s) 678, 1065
BseLI CCNNNNNNNGG 3 cut(s) 113, 767, 1076
BseMII CTCAG 2 cut(s) 351, 938
BseNI ACTGG 2 cut(s) 27, 733
BseRI GAGGAG 3 cut(s) 275, 420, 912
BseXI GCAGC 6 cut(s) 56, 59, 277, 593, 625, 949
BseYI CCCAGC 2 cut(s) 53, 422
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 2 cut(s) 57, 517
BsiHKAI GWGCWC 1 cut(s) 834
BslI CCNNNNNNNGG 3 cut(s) 113, 767, 1076
BsmAI GTCTC 1 cut(s) 858
BsmBI CGTCTC 1 cut(s) 858
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 834
Bsp143I GATC 5 cut(s) 529, 595, 673, 1147, 1168
BspACI CCGC 4 cut(s) 50, 304, 325, 538
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 2 cut(s) 350, 937
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 2 cut(s) 59, 519
BspMAI CTGCAG 2 cut(s) 46, 643
BspMI ACCTGC 2 cut(s) 632, 1093
BspPI GGATC 3 cut(s) 668, 1155, 1176
BspT107I GGYRCC 2 cut(s) 57, 517
BsrBI CCGCTC 2 cut(s) 304, 325
BsrI ACTGG 2 cut(s) 27, 733
BssECI CCNNGG 3 cut(s) 442, 513, 766
BssMI GATC 5 cut(s) 529, 595, 673, 1147, 1168
BssT1I CCWWGG 2 cut(s) 442, 766
Bst2UI CCWGG 4 cut(s) 164, 473, 515, 621
Bst4CI ACNGT 1 cut(s) 1039
BstBAI YACGTR 2 cut(s) 398, 650
BstC8I GCNNGC 3 cut(s) 522, 586, 967
BstDEI CTNAG 4 cut(s) 16, 337, 857, 924
BstF5I GGATG 9 cut(s) 10, 31, 122, 183, 867, 984, 1048, 1066, 1169
BstHHI GCGC 2 cut(s) 222, 760
BstKTI GATC 5 cut(s) 532, 598, 676, 1150, 1171
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 5 cut(s) 529, 595, 673, 1147, 1168
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 4 cut(s) 164, 473, 515, 621
BstNSI RCATGY 1 cut(s) 232
BstPAI GACNNNNGTC 1 cut(s) 850
BstSCI CCNGG 4 cut(s) 162, 471, 513, 619
BstSFI CTRYAG 2 cut(s) 42, 639
BstV1I GCAGC 6 cut(s) 56, 59, 277, 593, 625, 949
BsuRI GGCC 1 cut(s) 294
BtrI CACGTC 1 cut(s) 992
BtsCI GGATG 9 cut(s) 10, 31, 122, 183, 867, 984, 1048, 1066, 1169
BtsIMutI CAGTG 3 cut(s) 726, 1044, 1178
BveI ACCTGC 2 cut(s) 632, 1093
Cac8I GCNNGC 3 cut(s) 522, 586, 967
CaiI CAGNNNCTG 1 cut(s) 53
CciI TCATGA 1 cut(s) 148
CfoI GCGC 2 cut(s) 222, 760
Csp6I GTAC 2 cut(s) 58, 1094
CviAII CATG 5 cut(s) 149, 229, 782, 788, 884
CviQI GTAC 2 cut(s) 58, 1094
DdeI CTNAG 4 cut(s) 16, 337, 857, 924
DpnI GATC 5 cut(s) 531, 597, 675, 1149, 1170
DpnII GATC 5 cut(s) 529, 595, 673, 1147, 1168
EaeI YGGCCR 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 2 cut(s) 442, 766
Eco24I GRGCYC 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 2 cut(s) 584, 723
Eco72I CACGTG 2 cut(s) 398, 650
EcoICRI GAGCTC 1 cut(s) 832
EcoRII CCWGG 4 cut(s) 162, 471, 513, 619
EcoT14I CCWWGG 2 cut(s) 442, 766
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 2 cut(s) 442, 766
Esp3I CGTCTC 1 cut(s) 858
FaeI CATG 5 cut(s) 152, 232, 785, 791, 887
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FatI CATG 5 cut(s) 148, 228, 781, 787, 883
FauI CCCGC 1 cut(s) 545
Fnu4HI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
FokI GGATG 8 cut(s) 17, 38, 129, 190, 854, 971, 1053, 1055
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
FspBI CTAG 2 cut(s) 816, 897
GlaI GCGC 2 cut(s) 221, 759
GluI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
GsaI CCCAGC 2 cut(s) 57, 426
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 294
HhaI GCGC 2 cut(s) 222, 760
Hin1II CATG 5 cut(s) 152, 232, 785, 791, 887
Hin6I GCGC 2 cut(s) 220, 758
HinP1I GCGC 2 cut(s) 220, 758
HincII GTYRAC 2 cut(s) 724, 1035
HindII GTYRAC 2 cut(s) 724, 1035
HinfI GANTC 2 cut(s) 486, 1124
HphI GGTGA 2 cut(s) 96, 353
Hpy166II GTNNAC 3 cut(s) 647, 724, 1035
Hpy188I TCNGA 4 cut(s) 177, 485, 684, 989
Hpy188III TCNNGA 5 cut(s) 149, 434, 701, 871, 1070
Hpy8I GTNNAC 3 cut(s) 647, 724, 1035
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 4 cut(s) 370, 623, 698, 1022
HpyCH4III ACNGT 1 cut(s) 1039
HpyCH4IV ACGT 4 cut(s) 397, 590, 649, 991
HpyCH4V TGCA 6 cut(s) 44, 502, 588, 641, 737, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 4 cut(s) 16, 337, 857, 924
HpySE526I ACGT 4 cut(s) 397, 590, 649, 991
Hsp92II CATG 5 cut(s) 152, 232, 785, 791, 887
HspAI GCGC 2 cut(s) 220, 758
KpnI GGTACC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 529, 595, 673, 1147, 1168
LmnI GCTCC 3 cut(s) 262, 829, 925
Lsp1109I GCAGC 6 cut(s) 56, 59, 277, 593, 625, 949
MaeI CTAG 2 cut(s) 816, 897
MaeII ACGT 4 cut(s) 397, 590, 649, 991
MaeIII GTNAC 3 cut(s) 102, 206, 810
MalI GATC 5 cut(s) 531, 597, 675, 1149, 1170
MbiI CCGCTC 2 cut(s) 304, 325
MboI GATC 5 cut(s) 529, 595, 673, 1147, 1168
MboII GAAGA 3 cut(s) 206, 590, 1080
MhlI GDGCHC 1 cut(s) 834
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 6 cut(s) 35, 200, 401, 685, 979, 1128
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 1 cut(s) 495
MmeI TCCRAC 1 cut(s) 1012
MnlI CCTC 7 cut(s) 127, 218, 253, 441, 791, 867, 933
Mox20I TGGCCA 1 cut(s) 294
MroXI GAANNNNTTC 2 cut(s) 171, 713
MscI TGGCCA 1 cut(s) 294
MseI TTAA 2 cut(s) 38, 837
MslI CAYNNNNRTG 2 cut(s) 339, 786
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 4 cut(s) 164, 473, 515, 621
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 4 cut(s) 164, 473, 515, 621
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 5 cut(s) 529, 595, 673, 1147, 1168
NlaIII CATG 5 cut(s) 152, 232, 785, 791, 887
NlaIV GGNNCC 2 cut(s) 59, 519
NmuCI GTSAC 2 cut(s) 102, 206
NspI RCATGY 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 339
PagI TCATGA 1 cut(s) 148
PctI GAATGC 1 cut(s) 504
PdmI GAANNNNTTC 2 cut(s) 171, 713
PfeI GAWTC 1 cut(s) 1124
PfoI TCCNGGA 2 cut(s) 162, 471
PkrI GCNGC 7 cut(s) 46, 49, 52, 267, 583, 640, 964
PleI GAGTC 1 cut(s) 494
PmaCI CACGTG 2 cut(s) 398, 650
PmlI CACGTG 2 cut(s) 398, 650
PpsI GAGTC 1 cut(s) 494
Ppu21I YACGTR 2 cut(s) 398, 650
PshAI GACNNNNGTC 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 4 cut(s) 162, 471, 513, 619
PspCI CACGTG 2 cut(s) 398, 650
PspFI CCCAGC 2 cut(s) 53, 422
PspGI CCWGG 4 cut(s) 162, 471, 513, 619
PspN4I GGNNCC 2 cut(s) 59, 519
PstI CTGCAG 2 cut(s) 46, 643
PstNI CAGNNNCTG 1 cut(s) 53
RsaI GTAC 2 cut(s) 59, 1095
RsaNI GTAC 2 cut(s) 58, 1094
RseI CAYNNNNRTG 2 cut(s) 339, 786
SacI GAGCTC 1 cut(s) 834
SaqAI TTAA 2 cut(s) 38, 837
SatI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
Sau3AI GATC 5 cut(s) 529, 595, 673, 1147, 1168
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 4 cut(s) 164, 473, 515, 621
SduI GDGCHC 1 cut(s) 834
SfcI CTRYAG 2 cut(s) 42, 639
SmiMI CAYNNNNRTG 2 cut(s) 339, 786
Sse9I AATT 6 cut(s) 35, 200, 401, 685, 979, 1128
SsiI CCGC 4 cut(s) 50, 304, 325, 538
SspI AATATT 3 cut(s) 276, 286, 713
SspMI CTAG 2 cut(s) 816, 897
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 4 cut(s) 162, 471, 513, 619
StyI CCWWGG 2 cut(s) 442, 766
TaaI ACNGT 1 cut(s) 1039
TaiI ACGT 4 cut(s) 400, 593, 652, 994
TaqI TCGA 1 cut(s) 12
TasI AATT 6 cut(s) 35, 200, 401, 685, 979, 1128
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 1 cut(s) 1124
Tru1I TTAA 2 cut(s) 38, 837
Tru9I TTAA 2 cut(s) 38, 837
TscAI CASTG 3 cut(s) 733, 1044, 1178
TseFI GTSAC 2 cut(s) 102, 206
TseI GCWGC 6 cut(s) 44, 47, 265, 581, 638, 962
Tsp45I GTSAC 2 cut(s) 102, 206
TspDTI ATGAA 5 cut(s) 320, 758, 926, 1077, 1172
TspRI CASTG 3 cut(s) 733, 1044, 1178
XapI RAATTY 2 cut(s) 401, 685
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 2 cut(s) 171, 713
XspI CTAG 2 cut(s) 816, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.