RchiOBHm_Chr1g0319451

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007263
Physical Location & Seq
Forward (+)
7053359 .. 7059843
6485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
ATGACTGATTCTAAGCTATTCATATTTGGGTCATTCTCTGAAGATGAGACCAAGTCATTGCTTCTAAAGCAATCACCGGGGAAGCCGTCACCTGTGAAGGGTGAAAAGCCAGTGGAAACAAACGTATTGCAGTTTGGTTCGATAAATTTTGTTACGGATAAATCTTTAGTTGAGTTGAATGGGGGATCAAGTAGACAGCAAAGTTGTTTGAAGGGGCCGAGCAAATTGCAGTCTCTAAATGCAGATAATAAGCACAATGAAGTTAAGACTGCAAAAGCTGGAGATGATAATTTACTAGGATCAGTAGCAATTCCGACACAGAATGGGTGTGCTGATCATCACAGTCATAGTTCTACTCATAGTAATGGTGTAAAAGAGGTTACGACAGATGATATTGATTTATCTTCATCACATCTATCCCAGAATGAAGGTGGTTTCTCAAATCAATTTTCAAGTTTGAATTTGCAAGGCAGAGAGCAGAATGGTAGTGTTGGTGATTTATCAGTTTCTGATTCTAAGGGTGAACTCCAAAAGTCCTTGAACGGGCCTGCCACAGTCTGTACAATTTTGTTGCCTCGTGGTTTAATCAACTCCGGAAATCTCTGCTTTCTTAATGCGACTCTGCAGGCTCTCCTATCCTGTTCTCCTTTCGTTCAACTTCTTCATGAATTAAGAACTCGCAACATCCCCAAGATTGGCTACCCCACATTGTGTGCATTTGCTGAGTTTGTCTCTGAATTCGACGTGCCTAGTTCAAGTTCAAAGAATAAAGATACAAGTGTTGTCGAGGCTGGTAGGCCTCTTAGCCCTGCCATGTTTGAAGGTGTTCTTAAAGCTTTTACTCCAGACGTGCCAAGTACCATATCAGGCAGGCCAAGACAAGAAGATGCTCAGGAGTTTCTTAGCTTCATTATGGATCAGATGCATGATGAGTTACTTAAGCTTGAAGGACAATCCTCGTGCTTAAATGGGGGAAAATCATCTCTTGTATCTTCTGCAGAAGATGATGAATGGGAGACGGTTGGCCCAAAGAATACATCTGCAGTAACTAGAACACAGAGTTTTGTCCCTTCAGAATTGAGTGATATTTTTGGAGGACAATTAAGAAGTGTGGTGAAGGCAAGAGGAAATAAAGCTTCTGCTACTGTACAACCATATCTATTGCTGCACCTTGATATTAATCCTGAAGCTGTTCGTACTATTGAAGATGCACTTAAGTTGTTTTCTGCTCCAGAAACTCTTGAGGGATATCGAACATCAGCTGCGGGCAAGGCTGGTGTTGTGAGTGCAAGTAAATCAATAAAAATACAAAAGCTTTCAAAGATAATGATACTGCATTTGATGCGTTTTGGTTATGGAAGTCAAGGAAGCACCAAGTTGCATAAGCCTGTGCACTTTCCACTCGAATTGGTGTTTGGTCGTGAATTGCTTGTTTCTCCAACATCTGAGGGTCGAAAATACAAACTTGTTGCTACAATTACTCATCATGGGAGAGATCCTTCCAAGGGGCATTATACAACTGATGCTCTTTATGCTAGTGGTCAGTGGATCCGGTTTGATGATTCATCTGTCACAGCAATTGGGACCAAAAAAGTGCTGCATGACCAGGCATATGTCCTTATCTACAAACAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

545

Amino Acids

58.93

Weight (kDa)

6.77

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 193 - 542 3.6e-45 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 193
AccIII TCCGGA 1 cut(s) 593
AciI CCGC 1 cut(s) 1265
AclWI GGATC 6 cut(s) 193, 307, 924, 1490, 1543, 1556
AcsI RAATTY 3 cut(s) 145, 460, 737
AcuI CTGAAG 3 cut(s) 60, 1056, 1206
AdeI CACNNNGTG 1 cut(s) 711
AfaI GTAC 4 cut(s) 562, 859, 1149, 1198
AfiI CCNNNNNNNGG 4 cut(s) 98, 543, 695, 1505
AflII CTTAAG 2 cut(s) 938, 1214
AjiI CACGTC 2 cut(s) 745, 850
AjnI CCWGG 1 cut(s) 1605
AjuI GAANNNNNNNTTGG 2 cut(s) 1398, 1430
AluBI AGCT 9 cut(s) 16, 278, 836, 906, 943, 1136, 1190, 1262, 1315
AluI AGCT 9 cut(s) 16, 278, 836, 906, 943, 1136, 1190, 1262, 1315
Alw21I GWGCWC 1 cut(s) 1395
Alw26I GTCTC 4 cut(s) 41, 237, 736, 1010
Alw44I GTGCAC 1 cut(s) 1391
AlwI GGATC 6 cut(s) 193, 307, 924, 1490, 1543, 1556
AlwNI CAGNNNCTG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 593
AoxI GGCC 5 cut(s) 215, 545, 797, 872, 1024
ApaLI GTGCAC 1 cut(s) 1391
ApeKI GCWGC 3 cut(s) 1165, 1262, 1597
ApoI RAATTY 3 cut(s) 145, 460, 737
AseI ATTAAT 1 cut(s) 1179
Asp700I GAANNNNTTC 2 cut(s) 825, 1191
AspS9I GGNCC 4 cut(s) 215, 545, 1025, 1584
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 6 cut(s) 66, 81, 113, 506, 533, 1126
AvaII GGWCC 1 cut(s) 1584
BaeGI GKGCMC 1 cut(s) 1395
BaeI ACNNNNGTAYC 2 cut(s) 765, 798
BamHI GGATCC 1 cut(s) 1548
BauI CACGAG 2 cut(s) 576, 958
Bbv12I GWGCWC 1 cut(s) 1395
BbvI GCAGC 3 cut(s) 1152, 1249, 1584
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 1 cut(s) 1607
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 4 cut(s) 41, 237, 736, 1010
BfaI CTAG 4 cut(s) 296, 750, 1050, 1536
BfmI CTRYAG 3 cut(s) 623, 996, 1041
BfrI CTTAAG 2 cut(s) 938, 1214
BisI GCNGC 3 cut(s) 1166, 1263, 1598
BlsI GCNGC 3 cut(s) 1167, 1264, 1599
Bme1390I CCNGG 2 cut(s) 78, 1607
Bme18I GGWCC 1 cut(s) 1584
BmgBI CACGTC 2 cut(s) 745, 850
BmgT120I GGNCC 4 cut(s) 215, 545, 1025, 1584
BmiI GGNNCC 3 cut(s) 216, 1550, 1585
BmrFI CCNGG 2 cut(s) 78, 1607
BmsI GCATC 5 cut(s) 877, 912, 1198, 1332, 1513
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpmI CTGGAG 3 cut(s) 300, 828, 1215
Bpu10I CCTNAGC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 1262
BpuMI CCSGG 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 1179
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 77, 1503
BsaWI WCCGGW 2 cut(s) 593, 1551
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 543, 695, 1505
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 1179
BseAI TCCGGA 1 cut(s) 593
BseBI CCWGG 1 cut(s) 1607
BseDI CCNNGG 2 cut(s) 77, 1503
BseGI GGATG 1 cut(s) 684
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 4 cut(s) 98, 543, 695, 1505
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 3 cut(s) 714, 905, 1437
BseNI ACTGG 1 cut(s) 110
BseSI GKGCMC 1 cut(s) 1395
BseXI GCAGC 3 cut(s) 1152, 1249, 1584
BsgI GTGCAG 1 cut(s) 1151
BshFI GGCC 5 cut(s) 217, 547, 799, 874, 1026
BsiHKAI GWGCWC 1 cut(s) 1395
BsiSI CCGG 3 cut(s) 77, 594, 1552
BslFI GGGAC 2 cut(s) 1052, 1597
BslI CCNNNNNNNGG 4 cut(s) 98, 543, 695, 1505
BsmAI GTCTC 4 cut(s) 41, 237, 736, 1010
BsmBI CGTCTC 1 cut(s) 1010
BsmFI GGGAC 2 cut(s) 1052, 1597
BsnI GGCC 5 cut(s) 217, 547, 799, 874, 1026
Bso31I GGTCTC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 1395
Bsp13I TCCGGA 1 cut(s) 593
Bsp1407I TGTACA 2 cut(s) 560, 1147
Bsp143I GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
BspACI CCGC 1 cut(s) 1265
BspANI GGCC 5 cut(s) 217, 547, 799, 874, 1026
BspCNI CTCAG 3 cut(s) 715, 904, 1438
BspEI TCCGGA 1 cut(s) 593
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 3 cut(s) 216, 1550, 1585
BspMAI CTGCAG 3 cut(s) 627, 1000, 1045
BspPI GGATC 6 cut(s) 193, 307, 924, 1490, 1543, 1556
BspTI CTTAAG 2 cut(s) 938, 1214
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 56
BsrGI TGTACA 2 cut(s) 560, 1147
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 77, 1503
BssMI GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
BssSI CACGAG 2 cut(s) 576, 958
BssT1I CCWWGG 1 cut(s) 1503
Bst2BI CACGAG 2 cut(s) 576, 958
Bst2UI CCWGG 1 cut(s) 1607
Bst4CI ACNGT 5 cut(s) 344, 556, 1021, 1147, 1632
BstAFI CTTAAG 2 cut(s) 938, 1214
BstAPI GCANNNNNTGC 1 cut(s) 1342
BstAUI TGTACA 2 cut(s) 560, 1147
BstC8I GCNNGC 4 cut(s) 549, 627, 872, 1267
BstDEI CTNAG 7 cut(s) 12, 516, 723, 803, 891, 902, 1446
BstF5I GGATG 1 cut(s) 684
BstKTI GATC 6 cut(s) 188, 302, 337, 919, 1498, 1551
BstMAI GTCTC 4 cut(s) 41, 237, 736, 1010
BstMBI GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
BstMWI GCNNNNNNNGC 4 cut(s) 67, 1271, 1342, 1532
BstNI CCWGG 1 cut(s) 1607
BstSCI CCNGG 2 cut(s) 76, 1605
BstSFI CTRYAG 3 cut(s) 623, 996, 1041
BstSLI GKGCMC 1 cut(s) 1395
BstV1I GCAGC 3 cut(s) 1152, 1249, 1584
BstX2I RGATCY 2 cut(s) 1495, 1548
BstYI RGATCY 2 cut(s) 1495, 1548
BsuRI GGCC 5 cut(s) 217, 547, 799, 874, 1026
BtrI CACGTC 2 cut(s) 745, 850
BtsCI GGATG 1 cut(s) 684
BtsIMutI CAGTG 2 cut(s) 117, 1550
Cac8I GCNNGC 4 cut(s) 549, 627, 872, 1267
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 664
Cfr13I GGNCC 4 cut(s) 215, 545, 1025, 1584
Csp6I GTAC 4 cut(s) 561, 858, 1148, 1197
CspCI CAANNNNNGTGG 2 cut(s) 1389, 1424
CviAII CATG 5 cut(s) 665, 814, 926, 1487, 1601
CviQI GTAC 4 cut(s) 561, 858, 1148, 1197
DdeI CTNAG 7 cut(s) 12, 516, 723, 803, 891, 902, 1446
DpnI GATC 6 cut(s) 187, 301, 336, 918, 1497, 1550
DpnII GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
DraIII CACNNNGTG 1 cut(s) 711
Eco130I CCWWGG 1 cut(s) 1503
Eco147I AGGCCT 1 cut(s) 799
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 1250
Eco47I GGWCC 1 cut(s) 1584
Eco57I CTGAAG 3 cut(s) 60, 1056, 1206
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 1605
EcoRV GATATC 1 cut(s) 1250
EcoT14I CCWWGG 1 cut(s) 1503
EcoT22I ATGCAT 1 cut(s) 927
ErhI CCWWGG 1 cut(s) 1503
Esp3I CGTCTC 1 cut(s) 1010
FaeI CATG 5 cut(s) 668, 817, 929, 1490, 1604
FalI AAGNNNNNCTT 4 cut(s) 813, 845, 1197, 1229
FaqI GGGAC 2 cut(s) 1052, 1597
FatI CATG 5 cut(s) 664, 813, 925, 1486, 1600
FauI CCCGC 1 cut(s) 1258
FauNDI CATATG 1 cut(s) 1612
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 1166, 1263, 1598
FokI GGATG 1 cut(s) 671
Fsp4HI GCNGC 3 cut(s) 1166, 1263, 1598
FspBI CTAG 4 cut(s) 296, 750, 1050, 1536
GluI GCNGC 3 cut(s) 1166, 1263, 1598
GsuI CTGGAG 3 cut(s) 300, 828, 1215
HaeIII GGCC 5 cut(s) 217, 547, 799, 874, 1026
HapII CCGG 3 cut(s) 77, 594, 1552
Hin1II CATG 5 cut(s) 668, 817, 929, 1490, 1604
HindIII AAGCTT 4 cut(s) 834, 941, 1134, 1313
HinfI GANTC 4 cut(s) 8, 512, 619, 1562
HpaII CCGG 3 cut(s) 77, 594, 1552
HphI GGTGA 6 cut(s) 66, 81, 113, 506, 533, 1126
Hpy166II GTNNAC 3 cut(s) 194, 524, 1393
Hpy188I TCNGA 7 cut(s) 40, 315, 511, 736, 921, 1075, 1447
Hpy188III TCNNGA 8 cut(s) 594, 665, 845, 893, 1184, 1232, 1241, 1421
Hpy8I GTNNAC 3 cut(s) 194, 524, 1393
Hpy99I CGWCG 1 cut(s) 746
HpyAV CCTTC 8 cut(s) 91, 205, 422, 815, 941, 1080, 1111, 1509
HpyCH4III ACNGT 5 cut(s) 344, 556, 1021, 1147, 1632
HpyCH4IV ACGT 3 cut(s) 123, 744, 849
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 1271, 1342, 1532
HpyF3I CTNAG 7 cut(s) 12, 516, 723, 803, 891, 902, 1446
HpySE526I ACGT 3 cut(s) 123, 744, 849
Hsp92II CATG 5 cut(s) 668, 817, 929, 1490, 1604
Kpn2I TCCGGA 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
LmnI GCTCC 1 cut(s) 1234
Lsp1109I GCAGC 3 cut(s) 1152, 1249, 1584
LweI GCATC 5 cut(s) 877, 912, 1198, 1332, 1513
MaeI CTAG 4 cut(s) 296, 750, 1050, 1536
MaeII ACGT 3 cut(s) 123, 744, 849
MaeIII GTNAC 6 cut(s) 87, 151, 379, 933, 1045, 1570
MalI GATC 6 cut(s) 187, 301, 336, 918, 1497, 1550
MboI GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
MboII GAAGA 7 cut(s) 53, 396, 653, 896, 984, 1013, 1217
MfeI CAATTG 1 cut(s) 1578
MflI RGATCY 2 cut(s) 1495, 1548
MhlI GDGCHC 1 cut(s) 1395
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 2 cut(s) 338, 1463
MnlI CCTC 9 cut(s) 370, 585, 781, 810, 967, 1088, 1118, 1237, 1441
Mph1103I ATGCAT 1 cut(s) 927
MroI TCCGGA 1 cut(s) 593
MroXI GAANNNNTTC 2 cut(s) 825, 1191
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 1262
MspCI CTTAAG 2 cut(s) 938, 1214
MspI CCGG 3 cut(s) 77, 594, 1552
MspR9I CCNGG 2 cut(s) 78, 1607
MunI CAATTG 1 cut(s) 1578
MvaI CCWGG 1 cut(s) 1607
MwoI GCNNNNNNNGC 4 cut(s) 67, 1271, 1342, 1532
NciI CCSGG 1 cut(s) 78
NdeI CATATG 1 cut(s) 1612
NdeII GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
NlaIII CATG 5 cut(s) 668, 817, 929, 1490, 1604
NlaIV GGNNCC 3 cut(s) 216, 1550, 1585
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 87, 1570
NsiI ATGCAT 1 cut(s) 927
PagI TCATGA 1 cut(s) 664
PceI AGGCCT 1 cut(s) 799
PdmI GAANNNNTTC 2 cut(s) 825, 1191
PfeI GAWTC 3 cut(s) 8, 512, 1562
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 3 cut(s) 1167, 1264, 1599
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 1179
Psp6I CCWGG 1 cut(s) 1605
PspGI CCWGG 1 cut(s) 1605
PspN4I GGNNCC 3 cut(s) 216, 1550, 1585
PspPI GGNCC 4 cut(s) 215, 545, 1025, 1584
PstI CTGCAG 3 cut(s) 627, 1000, 1045
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 1495, 1548
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 4 cut(s) 562, 859, 1149, 1198
RsaNI GTAC 4 cut(s) 561, 858, 1148, 1197
RseI CAYNNNNRTG 1 cut(s) 363
SatI GCNGC 3 cut(s) 1166, 1263, 1598
Sau3AI GATC 6 cut(s) 185, 299, 334, 916, 1495, 1548
Sau96I GGNCC 4 cut(s) 215, 545, 1025, 1584
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 2 cut(s) 78, 1607
SduI GDGCHC 1 cut(s) 1395
SfaNI GCATC 5 cut(s) 877, 912, 1198, 1332, 1513
SfcI CTRYAG 3 cut(s) 623, 996, 1041
SinI GGWCC 1 cut(s) 1584
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 3 cut(s) 938, 1214, 1241
SmoI CTYRAG 3 cut(s) 938, 1214, 1241
SseBI AGGCCT 1 cut(s) 799
SsiI CCGC 1 cut(s) 1265
SspMI CTAG 4 cut(s) 296, 750, 1050, 1536
StuI AGGCCT 1 cut(s) 799
StyD4I CCNGG 2 cut(s) 76, 1605
StyI CCWWGG 1 cut(s) 1503
TaaI ACNGT 5 cut(s) 344, 556, 1021, 1147, 1632
TaiI ACGT 3 cut(s) 126, 747, 852
TaqI TCGA 6 cut(s) 140, 741, 786, 1252, 1404, 1453
TatI WGTACW 2 cut(s) 560, 1147
TfiI GAWTC 3 cut(s) 8, 512, 1562
TscAI CASTG 2 cut(s) 117, 1550
TseFI GTSAC 2 cut(s) 87, 1570
TseI GCWGC 3 cut(s) 1165, 1262, 1597
Tsp45I GTSAC 2 cut(s) 87, 1570
TspDTI ATGAA 9 cut(s) 10, 273, 396, 441, 653, 681, 898, 1023, 1554
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 117, 1550
Tth111I GACNNNGTC 1 cut(s) 52
Vha464I CTTAAG 2 cut(s) 938, 1214
VneI GTGCAC 1 cut(s) 1391
VpaK11BI GGWCC 1 cut(s) 1584
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 3 cut(s) 145, 460, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 193
XmnI GAANNNNTTC 2 cut(s) 825, 1191
XspI CTAG 4 cut(s) 296, 750, 1050, 1536
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.