Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Reverse (-)

31003971 .. 31020123

Gene Structure 16153 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3543 bp

ATGCAGCAGAAGAGGAGAACTTCCGCCGGAGACTTAAGCCACTTGCGTGAGCTGTTCCGGCACCACATAGAGTCATTCGATTACCTCATCACCGCCGGTCTCGGCGTCATGCTCCGTGACATCAAGCCCGTCCAAGTCCACAATCCTCACACCGGAGAAACCCTAAGGAATATCCTTTCTTTCATGATTCTATTCTCTTTTATATTACTCTGGTTCGCTGACGATCCTATTCTAGGCCGACCTCAGAAGGATCGGAGTAGCGAGCCTCTCTATCCTTTTGAATGTCGACAGACTCGAATTTCCTACACGGGCAAATTGTTGGCAGACATTTACTTTCAGCGTGACGATAAGGCTCCTGTCAGAGAGCAGTTAAATTTTGGCCAGTTTCCCATCATGCTCATGTCAAAGTGTTGCAACTTAAGAGGTCATGATCCTCAGCAGCTAGTTTCTTGCAAAGAAGAGTCATCAGAAATGGGTGGCTACTTCATTTTGAATGGGCTCGAGAGAGTTATTCGACCTGTTATATTGCCGAAGCGGAACTATCCAATGAGTACGGTGCGCAATTCATTTCGTGATCGAAAGGAAGGATATACAGATAAAGCAGTTGTGATAAGATGTGTAAGAGATGATCAGTCTTCAGTAACAGTCAAATTGTATTACCTGCAAAACGGAAGTGCAAGACTTGGTGTTTGGATACAGGGGAAGGAGTTCTTGCTTCCTGTGGGTCTTATTTTAAAGGCTTTTATTGACACTCCTGATCGTGAAATTTTTGCGAGTTTGACATGCTGTTGTAACGAGAAATTTGAGAAAGACAAAGGTGCTGTTGCCACTCAGCTTGTGGGTGAGAGGGCGCAAATTATTATAAATGAAGTGGCAGACTTGGGTCTTCGCACTCATCTTCAATGTCTAGAACACATTGGCGAGCACTTTCAACCTCTAATGGGTGGACTGGAGAATGAACCTTATTCAGTTGTTGGAGATGCGGTTCTCAAGGACTACATATTTGTTCACCTAGATAAGAATGAAGACAAATTCAATCTGCTCATATTCATGTTGCAGAAACTTTTTTCGCTTATGGATCAGACTTCGGTTCTCGACAACCCAGATTCCTTGCAAAATCAAGAAGTCTTACTCCCTGGCCATCTGATAACCATATATCTCAAGGAAAAATTAGAGGATTGGTTGCAAAGGACCAAAAAACTTCTTCAAGATGAGATGAAAAAGGAAAGCAACAATATCAATTTTGGCAGCGTCGATAGCTTAAAAAAGGTTCTGGAAAAAAATCCTACAAGGCAAATCAGTATGGCGATTGAGAATATGTTGAAAACTGGAAGAATTAATACTCAAACAGGTCTAGATTTGCAGCAGAGGGCGGGCTTTACAGTTCAGGCAGAAAGGCTTAACTTTTTACGATTTTTATCGCATTTTCGTGCTGTTCATCGAGGGGCTTCATTTGCTGGGCTTCGCACCACAAGTGTGCGAAAATTGCTTCCCGAATCCTGGGGTTTCCTTTGCCCTGTGCATACACCTGATGGAGAGCCATGTGGCTTGTTGAACCATATGACTCGCACTTGTAGAATTACATCGTACTTTGATGCACAAGGGAATATTAGAGATTTCTCTAAGATTCGAATGTCTATTCTTAATGTTATGATCGAAGTTGGAATGACACCTTCACTGCCAATGTTGGTTCATGCCGGTCCTCCTGCGGATCTTTCTGTTCTTTTAGATGGTCGTCTTGTTGGTTATATATCTTCCAAACAAGTTGAGAGAGTTGTGGCTCATTTAAGGAAGCTGAAAGTTTCAGCTGCTTCAGTGATTCCAGATGACTTGGAAGTGGGATACATTCCTCTCAGCATGGGTGGGGCATATCCTGGTCTCTACCTTAACACTTCTCCTTCTAGATTTGTTCGGCCGGTTAAGAATATTTCGATTCCTGCTGTTGAAGGTCAAAATATTGAACTCATTGGGCCCTTTGAACAGGTGTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGCTTTCCCTGCAACTCATGAAGAAATTCATCCAACTGGGATGCTCAGTGTTGTTGCTAATCTTACTCCCTGGTCAGAACATAATCAGAGCCCACGAAATATGTACCAGTGTCAGATGGCAAAACAAACGATGGGTTTCTCATTACAAGCACTTTGCAATCGTGCAGATCAGAAGTTGTATCATCTGAAGACTCCTCAATCTCCCATCGTCCGCACAAAAACGTATACGAATTACTGTGTAGATGAATATCCAACGGGCACAAATGCAATCGTTGCTGTATTGTCGTATACCGGGTATGATATGGAGGATGCTATGATTCTGAGCAAGTCATCTGTAGATCGTGGAATGTTTCATGGACTAGTATATCAGACGGAATCCATTGACTTATCTGATACAAAGGGTAGTAAATCCCAGAGATTGTTCAGGAGAAGTACCACTGATAAGTCACATCATTCTTTGATCGATTCAGATGGTCTTCCTTATGTGGGTCAGACAATAAAACCAGATGAACCCTATTATAGCACTTATGATGAGATAAAAGGTGCCATAAGAACGACCAAACGAAAGGGGTCGGAGCCTGCTATTGTTGACTATGTTGCTATTGATGGGAAGAAGCATCTTCAGAAGGTGAACATAAGGTTTCGGCACCCTAGAAACCCGATAATTGGTGATAAATTTAGCAGCCGACATGGACAAAAGGGAGTTTGCTCCCAGTTGTGGCCTGACATTGATATGCCATTTTCTGGAGTTACGGGAATGCGACCAGATCTTATTATCAATCCACATGCATTTCCTTCAAGAATGACAATAGCCATGCTTTTGGAGTCCGTTGCTGCAAAGGGTGGTAGCTTACATGGAAAATATGTTGATGCGACACCATTTGCTAATTCAGAGGAGAAAGTTAATGAAGAAACTAATGGAGAGAAATCCAAAACCCTTGTTGATGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAATTACCATGGTGCAGAGGTATTGTACAGTGGCGTCTATGGAACAGAACTCACGTGTGAAATCTTTATTGGTCCTGTTTACTATCAACGATTACGGCATATGGTTTCAGACAAATTTCAGGTTCGCTCTACGGGAATGGTAGATCAAGTCACACGCCAGCCTATCAAAGGACGAAAACGAGGTGGCGGTATACGTTTTGGTGAAATGGAGAGAGACTCCTTGCTTGCACATGGGGCAGCATATTTGTTGCATGATAGACTTCATACATGTTCTGATTATCACATTGCTGACGTATGTTCCCTTTGTGGATCCATCCTAACAACCTCATCAGTCCAGCCACAAATACGGGTTCGTGAAATGCGGGGCTTGCCCCCTATGAGAGCTCCCAAGAAGGTGACTTGCCATGCATGTAAGTCGAGTAAAGGGATGGAGACGGTTGCAATGCCTTATGTCTTTAGATATTTAGCAGCTGAGTTAGCAGCTATGAACATAAAACTGACCCTCCAGTTGAGCAATGCAACTGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1180

Amino Acids

132.92

Weight (kDa)

8.79

Isoelectric Point (pI)

37.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	18 - 411	8.9e-31	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	204 - 376	5.1e-10	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	464 - 527	1.2e-27	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	581 - 638	5.3e-21	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	696 - 1064	1.2e-121	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1066 - 1173	1.1e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	863
Acc16I	TGCGCA	1 cut(s)	560
Acc36I	ACCTGC	1 cut(s)	669
AccB1I	GGYRCC	3 cut(s)	60, 2576, 2679
AccB7I	CCANNNNNTGG	1 cut(s)	2777
AccI	GTMKAC	4 cut(s)	286, 2258, 2321, 3202
AciI	CCGC	9 cut(s)	24, 93, 535, 983, 1373, 1709, 2245, 3198, 3373
AclWI	GGATC	7 cut(s)	218, 258, 425, 1086, 1719, 3315, 3328
AcoI	YGGCCR	3 cut(s)	379, 1138, 1913
AcsI	RAATTY	8 cut(s)	297, 373, 765, 800, 1031, 2058, 2708, 3125
AcuI	CTGAAG	4 cut(s)	621, 1797, 2240, 2639
AcvI	CACGTG	1 cut(s)	3066
AcyI	GRCGYC	2 cut(s)	105, 3045
AfaI	GTAC	5 cut(s)	553, 1589, 2138, 2467, 3038
AfiI	CCNNNNNNNGG	9 cut(s)	152, 233, 941, 1500, 2519, 2699, 2751, 2777, 3179
AflII	CTTAAG	2 cut(s)	34, 418
AflIII	ACRYGT	2 cut(s)	3065, 3278
AhlI	ACTAGT	1 cut(s)	2392
AjnI	CCWGG	4 cut(s)	1135, 1499, 1873, 2102
AjuI	GAANNNNNNNTTGG	2 cut(s)	3063, 3095
AleI	CACNNNNGTG	2 cut(s)	45, 1475
AloI	GAACNNNNNNTCC	2 cut(s)	969, 1001
Alw21I	GWGCWC	2 cut(s)	927, 3397
Alw26I	GTCTC	5 cut(s)	24, 104, 1883, 3219, 3437
AlwI	GGATC	7 cut(s)	218, 258, 425, 1086, 1719, 3315, 3328
Ama87I	CYCGRG	1 cut(s)	500
AoxI	GGCC	6 cut(s)	235, 379, 1138, 1913, 1970, 2753
ApaI	GGGCCC	1 cut(s)	1974
ApoI	RAATTY	8 cut(s)	297, 373, 765, 800, 1031, 2058, 2708, 3125
ArsI	GACNNNNNNTTYG	4 cut(s)	672, 704, 986, 1018
AseI	ATTAAT	1 cut(s)	1338
Asp700I	GAANNNNTTC	2 cut(s)	707, 2058
AspLEI	GCGC	2 cut(s)	561, 853
AspS9I	GGNCC	5 cut(s)	1191, 1700, 1970, 1971, 3083
AsuC2I	CCSGG	1 cut(s)	2326
AsuHPI	GGTGA	8 cut(s)	82, 854, 1001, 2674, 2714, 2999, 3224, 3418
AsuII	TTCGAA	1 cut(s)	1630
AvaI	CYCGRG	1 cut(s)	500
AvaII	GGWCC	3 cut(s)	1191, 1700, 3083
AxyI	CCTNAGG	1 cut(s)	164
BaeGI	GKGCMC	2 cut(s)	1974, 2294
BalI	TGGCCA	2 cut(s)	381, 1140
BamHI	GGATCC	1 cut(s)	3320
BanI	GGYRCC	3 cut(s)	60, 2576, 2679
BanII	GRGCYC	4 cut(s)	501, 1974, 2126, 3397
BarI	GAAGNNNNNNTAC	2 cut(s)	1324, 1356
BbrPI	CACGTG	1 cut(s)	3066
BbsI	GAAGAC	5 cut(s)	627, 878, 1032, 2228, 2501
Bbv12I	GWGCWC	2 cut(s)	927, 3397
BbvCI	CCTCAGC	1 cut(s)	435
BceAI	ACGGC	1 cut(s)	3122
BcgI	CGANNNNNNTGC	2 cut(s)	3408, 3442
BciT130I	CCWGG	4 cut(s)	1137, 1501, 1875, 2104
BciVI	GTATCC	2 cut(s)	687, 1835
BclI	TGATCA	1 cut(s)	628
BcnI	CCSGG	1 cut(s)	2326
BcoDI	GTCTC	5 cut(s)	24, 104, 1883, 3219, 3437
BcuI	ACTAGT	1 cut(s)	2392
BfaI	CTAG	8 cut(s)	233, 443, 908, 1013, 1355, 1902, 2393, 2685
BfmI	CTRYAG	1 cut(s)	2367
BfrI	CTTAAG	2 cut(s)	34, 418
BfuAI	ACCTGC	1 cut(s)	669
BfuI	GTATCC	2 cut(s)	687, 1835
BglII	AGATCT	1 cut(s)	2800
Bme1390I	CCNGG	5 cut(s)	1137, 1501, 1875, 2104, 2326
Bme18I	GGWCC	3 cut(s)	1191, 1700, 3083
BmeT110I	CYCGRG	1 cut(s)	500
BmgT120I	GGNCC	5 cut(s)	1191, 1700, 1970, 1971, 3083
BmiI	GGNNCC	7 cut(s)	62, 354, 1972, 2578, 2610, 2681, 3322
BmrFI	CCNGG	5 cut(s)	1137, 1501, 1875, 2104, 2326
BmrI	ACTGGG	2 cut(s)	2079, 2740
BmsI	GCATC	7 cut(s)	970, 1585, 2023, 2064, 2332, 2659, 2893
BmuI	ACTGGG	2 cut(s)	2079, 2740
BoxI	GACNNNNGTC	1 cut(s)	882
BpiI	GAAGAC	5 cut(s)	627, 878, 1032, 2228, 2501
BplI	GAGNNNNNCTC	2 cut(s)	3212, 3244
BpmI	CTGGAG	3 cut(s)	971, 2799, 3500
Bpu10I	CCTNAGC	1 cut(s)	435
Bpu14I	TTCGAA	1 cut(s)	1630
BpuEI	CTTGAG	2 cut(s)	974, 1145
BpuMI	CCSGG	1 cut(s)	2326
Bsa29I	ATCGAT	1 cut(s)	2496
BsaAI	YACGTR	1 cut(s)	3066
BsaBI	GATNNNNATC	2 cut(s)	1417, 2280
BsaHI	GRCGYC	2 cut(s)	105, 3045
BsaI	GGTCTC	2 cut(s)	104, 1883
BsaJI	CCNNGG	4 cut(s)	1135, 1500, 2102, 3019
BsaWI	WCCGGW	1 cut(s)	152
BsaXI	ACNNNNNCTCC	4 cut(s)	969, 999, 3498, 3528
Bsc4I	CCNNNNNNNGG	9 cut(s)	152, 233, 941, 1500, 2519, 2699, 2751, 2777, 3179
Bse118I	RCCGGY	3 cut(s)	95, 1697, 1915
Bse1I	ACTGG	8 cut(s)	382, 954, 1333, 2074, 2140, 2746, 3517, 3538
Bse21I	CCTNAGG	1 cut(s)	164
Bse3DI	GCAATG	3 cut(s)	3294, 3459, 3532
Bse8I	GATNNNNATC	2 cut(s)	1417, 2280
BseBI	CCWGG	4 cut(s)	1137, 1501, 1875, 2104
BseCI	ATCGAT	1 cut(s)	2496
BseDI	CCNNGG	4 cut(s)	1135, 1500, 2102, 3019
BseGI	GGATG	5 cut(s)	2062, 2079, 2347, 3324, 3444
BseJI	GATNNNNATC	2 cut(s)	1417, 2280
BseLI	CCNNNNNNNGG	9 cut(s)	152, 233, 941, 1500, 2519, 2699, 2751, 2777, 3179
BseMI	GCAATG	3 cut(s)	3294, 3459, 3532
BseMII	CTCAG	7 cut(s)	257, 449, 845, 1867, 2092, 2345, 3474
BseNI	ACTGG	8 cut(s)	382, 954, 1333, 2074, 2140, 2746, 3517, 3538
BseRI	GAGGAG	3 cut(s)	28, 2217, 2942
BseSI	GKGCMC	2 cut(s)	1974, 2294
BseX3I	CGGCCG	1 cut(s)	1913
BseYI	CCCAGC	1 cut(s)	1457
BsgI	GTGCAG	2 cut(s)	2217, 3045
Bsh1285I	CGRYCG	1 cut(s)	1916
BshFI	GGCC	6 cut(s)	237, 381, 1140, 1915, 1972, 2755
BshNI	GGYRCC	3 cut(s)	60, 2576, 2679
BshVI	ATCGAT	1 cut(s)	2496
BsiEI	CGRYCG	1 cut(s)	1916
BsiHKAI	GWGCWC	2 cut(s)	927, 3397
BsiHKCI	CYCGRG	1 cut(s)	500
BsiSI	CCGG	7 cut(s)	27, 58, 96, 153, 1698, 1916, 2325
BslI	CCNNNNNNNGG	9 cut(s)	152, 233, 941, 1500, 2519, 2699, 2751, 2777, 3179
BsmAI	GTCTC	5 cut(s)	24, 104, 1883, 3219, 3437
BsmBI	CGTCTC	1 cut(s)	3437
BsmI	GAATGC	1 cut(s)	2796
BsnI	GGCC	6 cut(s)	237, 381, 1140, 1915, 1972, 2755
Bso31I	GGTCTC	2 cut(s)	104, 1883
BsoBI	CYCGRG	1 cut(s)	500
Bsp119I	TTCGAA	1 cut(s)	1630
Bsp120I	GGGCCC	1 cut(s)	1970
Bsp1286I	GDGCHC	6 cut(s)	501, 927, 1974, 2126, 2294, 3397
Bsp1407I	TGTACA	1 cut(s)	3036
Bsp19I	CCATGG	1 cut(s)	3019
BspACI	CCGC	9 cut(s)	24, 93, 535, 983, 1373, 1709, 2245, 3198, 3373
BspANI	GGCC	6 cut(s)	237, 381, 1140, 1915, 1972, 2755
BspCNI	CTCAG	7 cut(s)	256, 448, 844, 1866, 2091, 2346, 3475
BspDI	ATCGAT	1 cut(s)	2496
BspHI	TCATGA	3 cut(s)	183, 427, 2050
BspLI	GGNNCC	7 cut(s)	62, 354, 1972, 2578, 2610, 2681, 3322
BspMI	ACCTGC	1 cut(s)	669
BspPI	GGATC	7 cut(s)	218, 258, 425, 1086, 1719, 3315, 3328
BspT104I	TTCGAA	1 cut(s)	1630
BspT107I	GGYRCC	3 cut(s)	60, 2576, 2679
BspTI	CTTAAG	2 cut(s)	34, 418
BspTNI	GGTCTC	2 cut(s)	104, 1883
BsrDI	GCAATG	3 cut(s)	3294, 3459, 3532
BsrFI	RCCGGY	3 cut(s)	95, 1697, 1915
BsrGI	TGTACA	1 cut(s)	3036
BsrI	ACTGG	8 cut(s)	382, 954, 1333, 2074, 2140, 2746, 3517, 3538
BssAI	RCCGGY	3 cut(s)	95, 1697, 1915
BssECI	CCNNGG	4 cut(s)	1135, 1500, 2102, 3019
BssNAI	GTATAC	3 cut(s)	2259, 2322, 3203
BssNI	GRCGYC	2 cut(s)	105, 3045
BssT1I	CCWWGG	1 cut(s)	3019
Bst1107I	GTATAC	3 cut(s)	2259, 2322, 3203
Bst2UI	CCWGG	4 cut(s)	1137, 1501, 1875, 2104
Bst4CI	ACNGT	6 cut(s)	556, 646, 1384, 2270, 3041, 3448
Bst6I	CTCTTC	2 cut(s)	5, 453
BstACI	GRCGYC	2 cut(s)	105, 3045
BstAFI	CTTAAG	2 cut(s)	34, 418
BstAPI	GCANNNNNTGC	1 cut(s)	2306
BstAUI	TGTACA	1 cut(s)	3036
BstBAI	YACGTR	1 cut(s)	3066
BstBI	TTCGAA	1 cut(s)	1630
BstC8I	GCNNGC	7 cut(s)	263, 923, 1375, 2613, 3170, 3237, 3380
BstDEI	CTNAG	9 cut(s)	164, 243, 435, 831, 1623, 1853, 2078, 2354, 3483
BstDSI	CCRYGG	1 cut(s)	3019
BstENI	CCTNNNNNAGG	2 cut(s)	231, 3177
BstF5I	GGATG	5 cut(s)	2062, 2079, 2347, 3324, 3444
BstHHI	GCGC	2 cut(s)	561, 853
BstMAI	GTCTC	5 cut(s)	24, 104, 1883, 3219, 3437
BstMCI	CGRYCG	1 cut(s)	1916
BstNI	CCWGG	4 cut(s)	1137, 1501, 1875, 2104
BstNSI	RCATGY	4 cut(s)	786, 2822, 3282, 3423
BstPAI	GACNNNNGTC	1 cut(s)	882
BstSCI	CCNGG	5 cut(s)	1135, 1499, 1873, 2102, 2324
BstSFI	CTRYAG	1 cut(s)	2367
BstSLI	GKGCMC	2 cut(s)	1974, 2294
BstV2I	GAAGAC	5 cut(s)	627, 878, 1032, 2228, 2501
BstX2I	RGATCY	3 cut(s)	1711, 2800, 3320
BstXI	CCANNNNNNTGG	1 cut(s)	2854
BstYI	RGATCY	3 cut(s)	1711, 2800, 3320
BstZ17I	GTATAC	3 cut(s)	2259, 2322, 3203
BstZI	CGGCCG	1 cut(s)	1913
Bsu15I	ATCGAT	1 cut(s)	2496
Bsu36I	CCTNAGG	1 cut(s)	164
BsuI	GTATCC	2 cut(s)	687, 1835
BsuRI	GGCC	6 cut(s)	237, 381, 1140, 1915, 1972, 2755
BsuTUI	ATCGAT	1 cut(s)	2496
BtgI	CCRYGG	1 cut(s)	3019
BtsCI	GGATG	5 cut(s)	2062, 2079, 2347, 3324, 3444
BtsI	GCAGTG	1 cut(s)	1676
BtsIMutI	CAGTG	6 cut(s)	1676, 1821, 2086, 2147, 2469, 3046
BveI	ACCTGC	1 cut(s)	669
Cac8I	GCNNGC	7 cut(s)	263, 923, 1375, 2613, 3170, 3237, 3380
CciI	TCATGA	3 cut(s)	183, 427, 2050
CfoI	GCGC	2 cut(s)	561, 853
Cfr10I	RCCGGY	3 cut(s)	95, 1697, 1915
Cfr13I	GGNCC	5 cut(s)	1191, 1700, 1970, 1971, 3083
ClaI	ATCGAT	1 cut(s)	2496
CseI	GACGC	3 cut(s)	94, 1240, 3034
Csp6I	GTAC	5 cut(s)	552, 1588, 2137, 2466, 3037
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviQI	GTAC	5 cut(s)	552, 1588, 2137, 2466, 3037
DdeI	CTNAG	9 cut(s)	164, 243, 435, 831, 1623, 1853, 2078, 2354, 3483
DraI	TTTAAA	1 cut(s)	735
EaeI	YGGCCR	3 cut(s)	379, 1138, 1913
EagI	CGGCCG	1 cut(s)	1913
Eam1104I	CTCTTC	2 cut(s)	5, 453
EarI	CTCTTC	2 cut(s)	5, 453
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	3395
EclXI	CGGCCG	1 cut(s)	1913
Eco130I	CCWWGG	1 cut(s)	3019
Eco24I	GRGCYC	4 cut(s)	501, 1974, 2126, 3397
Eco31I	GGTCTC	2 cut(s)	104, 1883
Eco47I	GGWCC	3 cut(s)	1191, 1700, 3083
Eco52I	CGGCCG	1 cut(s)	1913
Eco53kI	GAGCTC	1 cut(s)	3395
Eco57I	CTGAAG	4 cut(s)	621, 1797, 2240, 2639
Eco72I	CACGTG	1 cut(s)	3066
Eco81I	CCTNAGG	1 cut(s)	164
Eco88I	CYCGRG	1 cut(s)	500
EcoICRI	GAGCTC	1 cut(s)	3395
EcoNI	CCTNNNNNAGG	2 cut(s)	231, 3177
EcoO109I	RGGNCCY	1 cut(s)	1971
EcoRII	CCWGG	4 cut(s)	1135, 1499, 1873, 2102
EcoT14I	CCWWGG	1 cut(s)	3019
EcoT22I	ATGCAT	2 cut(s)	2824, 3421
EcoT38I	GRGCYC	4 cut(s)	501, 1974, 2126, 3397
ErhI	CCWWGG	1 cut(s)	3019
Esp3I	CGTCTC	1 cut(s)	3437
FalI	AAGNNNNNCTT	2 cut(s)	695, 727
FauI	CCCGC	2 cut(s)	1366, 3366
FauNDI	CATATG	2 cut(s)	1560, 3111
FbaI	TGATCA	1 cut(s)	628
FblI	GTMKAC	4 cut(s)	286, 2258, 2321, 3202
FokI	GGATG	5 cut(s)	2049, 2086, 2354, 3311, 3451
FriOI	GRGCYC	4 cut(s)	501, 1974, 2126, 3397
FspBI	CTAG	8 cut(s)	233, 443, 908, 1013, 1355, 1902, 2393, 2685
FspI	TGCGCA	1 cut(s)	560
GlaI	GCGC	2 cut(s)	560, 852
GsaI	CCCAGC	1 cut(s)	1461
GsuI	CTGGAG	3 cut(s)	971, 2799, 3500
HaeIII	GGCC	6 cut(s)	237, 381, 1140, 1915, 1972, 2755
HapII	CCGG	7 cut(s)	27, 58, 96, 153, 1698, 1916, 2325
HgaI	GACGC	3 cut(s)	94, 1240, 3034
HhaI	GCGC	2 cut(s)	561, 853
Hin1I	GRCGYC	2 cut(s)	105, 3045
Hin6I	GCGC	2 cut(s)	559, 851
HinP1I	GCGC	2 cut(s)	559, 851
HincII	GTYRAC	2 cut(s)	287, 2623
HindII	GTYRAC	2 cut(s)	287, 2623
HpaII	CCGG	7 cut(s)	27, 58, 96, 153, 1698, 1916, 2325
HphI	GGTGA	8 cut(s)	82, 854, 1001, 2674, 2714, 2999, 3224, 3418
Hpy99I	CGWCG	1 cut(s)	1256
HpyCH4III	ACNGT	6 cut(s)	556, 646, 1384, 2270, 3041, 3448
HpyCH4IV	ACGT	4 cut(s)	2255, 3065, 3205, 3303
HpyF3I	CTNAG	9 cut(s)	164, 243, 435, 831, 1623, 1853, 2078, 2354, 3483
HpySE526I	ACGT	4 cut(s)	2255, 3065, 3205, 3303
Hsp92I	GRCGYC	2 cut(s)	105, 3045
HspAI	GCGC	2 cut(s)	559, 851
Ksp22I	TGATCA	1 cut(s)	628
LmnI	GCTCC	6 cut(s)	117, 358, 2608, 2747, 3400, 3536
LweI	GCATC	7 cut(s)	970, 1585, 2023, 2064, 2332, 2659, 2893
MaeI	CTAG	8 cut(s)	233, 443, 908, 1013, 1355, 1902, 2393, 2685
MaeII	ACGT	4 cut(s)	2255, 3065, 3205, 3303
MaeIII	GTNAC	8 cut(s)	116, 341, 640, 791, 2478, 2782, 3160, 3406
MflI	RGATCY	3 cut(s)	1711, 2800, 3320
MhlI	GDGCHC	6 cut(s)	501, 927, 1974, 2126, 2294, 3397
MlsI	TGGCCA	2 cut(s)	381, 1140
MluNI	TGGCCA	2 cut(s)	381, 1140
MlyI	GAGTC	7 cut(s)	80, 286, 470, 1558, 2218, 2867, 3221
MmeI	TCCRAC	5 cut(s)	955, 1642, 2090, 2309, 2586
Mox20I	TGGCCA	2 cut(s)	381, 1140
Mph1103I	ATGCAT	2 cut(s)	2824, 3421
MroXI	GAANNNNTTC	2 cut(s)	707, 2058
MscI	TGGCCA	2 cut(s)	381, 1140
MslI	CAYNNNNRTG	6 cut(s)	45, 398, 1049, 1428, 1475, 2765
Msp20I	TGGCCA	2 cut(s)	381, 1140
MspA1I	CMGCKG	2 cut(s)	1808, 3482
MspCI	CTTAAG	2 cut(s)	34, 418
MspI	CCGG	7 cut(s)	27, 58, 96, 153, 1698, 1916, 2325
MspR9I	CCNGG	5 cut(s)	1137, 1501, 1875, 2104, 2326
Mva1269I	GAATGC	1 cut(s)	2796
MvaI	CCWGG	4 cut(s)	1137, 1501, 1875, 2104
NciI	CCSGG	1 cut(s)	2326
NcoI	CCATGG	1 cut(s)	3019
NdeI	CATATG	2 cut(s)	1560, 3111
NlaIV	GGNNCC	7 cut(s)	62, 354, 1972, 2578, 2610, 2681, 3322
NmeAIII	GCCGAG	1 cut(s)	81
NmuCI	GTSAC	5 cut(s)	116, 341, 2478, 3160, 3406
NsbI	TGCGCA	1 cut(s)	560
NsiI	ATGCAT	2 cut(s)	2824, 3421
NspI	RCATGY	4 cut(s)	786, 2822, 3282, 3423
NspV	TTCGAA	1 cut(s)	1630
OliI	CACNNNNGTG	2 cut(s)	45, 1475
PaeR7I	CTCGAG	1 cut(s)	500
PagI	TCATGA	3 cut(s)	183, 427, 2050
PciI	ACATGT	1 cut(s)	3278
PcsI	WCGNNNNNNNCGW	1 cut(s)	292
PctI	GAATGC	1 cut(s)	2796
PdmI	GAANNNNTTC	2 cut(s)	707, 2058
PfeI	GAWTC	9 cut(s)	187, 1106, 1496, 1627, 1819, 1933, 2350, 2408, 2498
PflMI	CCANNNNNTGG	1 cut(s)	2777
PleI	GAGTC	7 cut(s)	79, 286, 469, 1558, 2218, 2866, 3221
PmaCI	CACGTG	1 cut(s)	3066
PmlI	CACGTG	1 cut(s)	3066
PpsI	GAGTC	7 cut(s)	79, 286, 469, 1558, 2218, 2866, 3221
Ppu21I	YACGTR	1 cut(s)	3066
PscI	ACATGT	1 cut(s)	3278
PshAI	GACNNNNGTC	1 cut(s)	882
PshBI	ATTAAT	1 cut(s)	1338
PsiI	TTATAA	1 cut(s)	863
Psp124BI	GAGCTC	1 cut(s)	3397
Psp6I	CCWGG	4 cut(s)	1135, 1499, 1873, 2102
PspCI	CACGTG	1 cut(s)	3066
PspFI	CCCAGC	1 cut(s)	1457
PspGI	CCWGG	4 cut(s)	1135, 1499, 1873, 2102
PspN4I	GGNNCC	7 cut(s)	62, 354, 1972, 2578, 2610, 2681, 3322
PspOMI	GGGCCC	1 cut(s)	1970
PspPI	GGNCC	5 cut(s)	1191, 1700, 1970, 1971, 3083
PsuI	RGATCY	3 cut(s)	1711, 2800, 3320
PvuII	CAGCTG	2 cut(s)	1808, 3482
RsaI	GTAC	5 cut(s)	553, 1589, 2138, 2467, 3038
RsaNI	GTAC	5 cut(s)	552, 1588, 2137, 2466, 3037
RseI	CAYNNNNRTG	6 cut(s)	45, 398, 1049, 1428, 1475, 2765
SacI	GAGCTC	1 cut(s)	3397
SalI	GTCGAC	1 cut(s)	285
Sau96I	GGNCC	5 cut(s)	1191, 1700, 1970, 1971, 3083
SchI	GAGTC	7 cut(s)	80, 286, 470, 1558, 2218, 2867, 3221
ScrFI	CCNGG	5 cut(s)	1137, 1501, 1875, 2104, 2326
SduI	GDGCHC	6 cut(s)	501, 927, 1974, 2126, 2294, 3397
SfaNI	GCATC	7 cut(s)	970, 1585, 2023, 2064, 2332, 2659, 2893
SfcI	CTRYAG	1 cut(s)	2367
Sfr274I	CTCGAG	1 cut(s)	500
SfuI	TTCGAA	1 cut(s)	1630
SinI	GGWCC	3 cut(s)	1191, 1700, 3083
SlaI	CTCGAG	1 cut(s)	500
SmiMI	CAYNNNNRTG	6 cut(s)	45, 398, 1049, 1428, 1475, 2765
SmlI	CTYRAG	5 cut(s)	34, 418, 500, 989, 1160
SmoI	CTYRAG	5 cut(s)	34, 418, 500, 989, 1160
SpeI	ACTAGT	1 cut(s)	2392
SsiI	CCGC	9 cut(s)	24, 93, 535, 983, 1373, 1709, 2245, 3198, 3373
SspI	AATATT	3 cut(s)	1609, 1927, 1957
SspMI	CTAG	8 cut(s)	233, 443, 908, 1013, 1355, 1902, 2393, 2685
SstI	GAGCTC	1 cut(s)	3397
StyD4I	CCNGG	5 cut(s)	1135, 1499, 1873, 2102, 2324
StyI	CCWWGG	1 cut(s)	3019
TaaI	ACNGT	6 cut(s)	556, 646, 1384, 2270, 3041, 3448
TaiI	ACGT	4 cut(s)	2258, 3068, 3208, 3306
TatI	WGTACW	1 cut(s)	3036
TfiI	GAWTC	9 cut(s)	187, 1106, 1496, 1627, 1819, 1933, 2350, 2408, 2498
TscAI	CASTG	6 cut(s)	1683, 1821, 2086, 2147, 2476, 3046
TseFI	GTSAC	5 cut(s)	116, 341, 2478, 3160, 3406
Tsp45I	GTSAC	5 cut(s)	116, 341, 2478, 3160, 3406
TspGWI	ACGGA	4 cut(s)	104, 684, 2420, 2851
TspRI	CASTG	6 cut(s)	1683, 1821, 2086, 2147, 2476, 3046
Van91I	CCANNNNNTGG	1 cut(s)	2777
Vha464I	CTTAAG	2 cut(s)	34, 418
VpaK11BI	GGWCC	3 cut(s)	1191, 1700, 3083
VspI	ATTAAT	1 cut(s)	1338
XagI	CCTNNNNNAGG	2 cut(s)	231, 3177
XapI	RAATTY	8 cut(s)	297, 373, 765, 800, 1031, 2058, 2708, 3125
XbaI	TCTAGA	3 cut(s)	907, 1354, 1901
XceI	RCATGY	4 cut(s)	786, 2822, 3282, 3423
XcmI	CCANNNNNNNNNTGG	1 cut(s)	835
XhoI	CTCGAG	1 cut(s)	500
XmiI	GTMKAC	4 cut(s)	286, 2258, 2321, 3202
XmnI	GAANNNNTTC	2 cut(s)	707, 2058
XspI	CTAG	8 cut(s)	233, 443, 908, 1013, 1355, 1902, 2393, 2685
Zsp2I	ATGCAT	2 cut(s)	2824, 3421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.