RchiOBHm_Chr1g0351961

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007611
Physical Location & Seq
Reverse (-)
45101610 .. 45102158
549 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGGAGCCAAAGACGAAAAGTGACCCGGTAGTGGAATGTGATGATGCTCAGAACCTTCATACACTAACTGAAGGGAGCGCTAGGCCCAGTGCTCATAATCATATTGATGAGCAGGTGGCTCGTGACCCGGCCTTATACAAAATAAAGAAGAAGTTGACTCTAAGTGACCTCGGAAACTTGAGCAGGCTCTTGGTGCCAAAAGAGATGGTCAAGAACCATGTTCTGCCTCACGTGAATGGCGACTTTGTACAAAGACTCGAGAGCAAAGCCGGTAAACAAGTTACGGTTAAAGACTCTGACAAAGAGACCGAGCATCAATTAACTTTTAGATACTTAAGGTCGTCGAAGAGTTATGTTTTCAATGGAAATTGGATGGAAATCGTGAAGGGGAGAGATTTGAAGAAGGATGATGAAATTGGACTTTACTGGGACACACTCAATTCCAATTTCCACTTTTCTCTTCTGAAACTTGCAAGCCAAGAAAGCCAAGCTGCCGATGAAGTTACTCGCGCGTGGTCATCAACAATGACATCAAGTCTAGAATCTTAG

Protein Analysis

182

Amino Acids

20.66

Weight (kDa)

6.38

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 50 - 151 1.4e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 193
AccII CGCG 2 cut(s) 510, 512
AcuI CTGAAG 1 cut(s) 90
AcvI CACGTG 1 cut(s) 232
AfaI GTAC 1 cut(s) 249
AfeI AGCGCT 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 31
AflII CTTAAG 1 cut(s) 334
AgsI TTSAA 2 cut(s) 361, 400
AhdI GACNNNNNGTC 1 cut(s) 534
AluBI AGCT 1 cut(s) 491
AluI AGCT 1 cut(s) 491
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 257
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 2 cut(s) 83, 129
ApeKI GCWGC 1 cut(s) 491
AspLEI GCGC 2 cut(s) 80, 512
AspS9I GGNCC 1 cut(s) 84
AsuC2I CCSGG 2 cut(s) 26, 128
AvaI CYCGRG 1 cut(s) 257
BanI GGYRCC 1 cut(s) 193
BauI CACGAG 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 199, 367
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BcnI CCSGG 2 cut(s) 26, 128
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 81, 539
BfoI RGCGCY 1 cut(s) 81
BfrI CTTAAG 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 2 cut(s) 26, 128
BmeRI GACNNNNNGTC 1 cut(s) 534
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 2 cut(s) 6, 195
BmrFI CCNGG 2 cut(s) 26, 128
BmrI ACTGGG 2 cut(s) 81, 436
BmsI GCATC 2 cut(s) 34, 322
BmuI ACTGGG 2 cut(s) 81, 436
BpuEI CTTGAG 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 26, 128
BsaAI YACGTR 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 87, 431
Bse8I GATNNNNATC 1 cut(s) 377
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 2 cut(s) 378, 412
BseJI GATNNNNATC 1 cut(s) 377
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 2 cut(s) 87, 431
BseXI GCAGC 1 cut(s) 478
Bsh1236I CGCG 2 cut(s) 510, 512
BshFI GGCC 2 cut(s) 85, 131
BshNI GGYRCC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 26, 128, 270
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 2 cut(s) 85, 131
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 247
BspANI GGCC 2 cut(s) 85, 131
BspCNI CTCAG 1 cut(s) 61
BspFNI CGCG 2 cut(s) 510, 512
BspLI GGNNCC 2 cut(s) 6, 195
BspMI ACCTGC 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 193
BspTI CTTAAG 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 247
BsrI ACTGG 2 cut(s) 87, 431
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 169
BssSI CACGAG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 2 cut(s) 341, 465
BstAFI CTTAAG 1 cut(s) 334
BstAUI TGTACA 1 cut(s) 247
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 185, 475
BstDEI CTNAG 3 cut(s) 48, 161, 546
BstF5I GGATG 2 cut(s) 378, 412
BstFNI CGCG 2 cut(s) 510, 512
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 2 cut(s) 80, 512
BstMAI GTCTC 1 cut(s) 299
BstMWI GCNNNNNNNGC 2 cut(s) 193, 483
BstSCI CCNGG 2 cut(s) 24, 126
BstUI CGCG 2 cut(s) 510, 512
BstV1I GCAGC 1 cut(s) 478
BsuRI GGCC 2 cut(s) 85, 131
BtsCI GGATG 2 cut(s) 378, 412
BtsIMutI CAGTG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 185, 475
CfoI GCGC 2 cut(s) 80, 512
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 218
CviJI RGCY 9 cut(s) 7, 85, 119, 131, 187, 269, 477, 486, 491
CviKI_1 RGCY 9 cut(s) 7, 85, 119, 131, 187, 269, 477, 486, 491
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 3 cut(s) 48, 161, 546
DriI GACNNNNNGTC 1 cut(s) 534
Eam1104I CTCTTC 2 cut(s) 341, 465
Eam1105I GACNNNNNGTC 1 cut(s) 534
EarI CTCTTC 2 cut(s) 341, 465
Eco31I GGTCTC 1 cut(s) 299
Eco47III AGCGCT 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 90
Eco72I CACGTG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 257
FaeI CATG 1 cut(s) 221
FaiI YATR 6 cut(s) 60, 96, 102, 136, 219, 354
FaqI GGGAC 1 cut(s) 443
FatI CATG 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 2 cut(s) 385, 419
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 81, 539
GlaI GCGC 2 cut(s) 79, 511
GluI GCNGC 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 85, 131
HapII CCGG 3 cut(s) 26, 128, 270
HhaI GCGC 2 cut(s) 80, 512
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 2 cut(s) 78, 510
HinP1I GCGC 2 cut(s) 78, 510
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 4 cut(s) 157, 255, 293, 542
HpaII CCGG 3 cut(s) 26, 128, 270
Hpy166II GTNNAC 2 cut(s) 156, 275
Hpy188I TCNGA 4 cut(s) 51, 173, 298, 465
Hpy188III TCNNGA 5 cut(s) 122, 211, 259, 382, 539
Hpy8I GTNNAC 2 cut(s) 156, 275
Hpy99I CGWCG 1 cut(s) 346
HpyAV CCTTC 4 cut(s) 65, 65, 379, 397
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 483
HpyF3I CTNAG 3 cut(s) 48, 161, 546
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 2 cut(s) 78, 510
LmnI GCTCC 2 cut(s) 4, 75
LpnPI CCDG 7 cut(s) 39, 98, 100, 141, 169, 283, 412
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 2 cut(s) 34, 322
MaeI CTAG 2 cut(s) 81, 539
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 5 cut(s) 20, 122, 164, 280, 502
MboII GAAGA 4 cut(s) 160, 358, 412, 452
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 5 cut(s) 317, 367, 414, 439, 445
MlyI GAGTC 3 cut(s) 151, 249, 287
MnlI CCTC 2 cut(s) 179, 237
MseI TTAA 3 cut(s) 288, 320, 335
MslI CAYNNNNRTG 2 cut(s) 105, 234
MspCI CTTAAG 1 cut(s) 334
MspI CCGG 3 cut(s) 26, 128, 270
MspR9I CCNGG 2 cut(s) 26, 128
MvnI CGCG 2 cut(s) 510, 512
MwoI GCNNNNNNNGC 2 cut(s) 193, 483
NciI CCSGG 2 cut(s) 26, 128
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 2 cut(s) 6, 195
NmuCI GTSAC 3 cut(s) 20, 122, 164
PaeR7I CTCGAG 1 cut(s) 257
PaqCI CACCTGC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 542
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 3 cut(s) 151, 249, 287
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
PpsI GAGTC 3 cut(s) 151, 249, 287
Ppu21I YACGTR 1 cut(s) 232
PspCI CACGTG 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 6, 195
PspPI GGNCC 1 cut(s) 84
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 2 cut(s) 105, 234
SaqAI TTAA 3 cut(s) 288, 320, 335
SatI GCNGC 1 cut(s) 492
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 3 cut(s) 151, 249, 287
ScrFI CCNGG 2 cut(s) 26, 128
SduI GDGCHC 1 cut(s) 94
SetI ASST 6 cut(s) 57, 117, 171, 234, 341, 493
SfaNI GCATC 2 cut(s) 34, 322
Sfr274I CTCGAG 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 2 cut(s) 105, 234
SmlI CTYRAG 3 cut(s) 178, 257, 334
SmoI CTYRAG 3 cut(s) 178, 257, 334
Sse9I AATT 5 cut(s) 317, 367, 414, 439, 445
SspMI CTAG 2 cut(s) 81, 539
StyD4I CCNGG 2 cut(s) 24, 126
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 258, 344
TaqII GACCGA 1 cut(s) 323
TasI AATT 5 cut(s) 317, 367, 414, 439, 445
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 1 cut(s) 542
Tru1I TTAA 3 cut(s) 288, 320, 335
Tru9I TTAA 3 cut(s) 288, 320, 335
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 3 cut(s) 20, 122, 164
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 3 cut(s) 20, 122, 164
TspDTI ATGAA 3 cut(s) 47, 426, 513
TspRI CASTG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 334
XbaI TCTAGA 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 257
XspI CTAG 2 cut(s) 81, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.