RchiOBHm_Chr1g0364181

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003478
Physical Location & Seq
Reverse (-)
55427126 .. 55434234
7109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3600 bp
ATGGACGAAAGCAAAGACAGGGGTGTTGCTCGTGAAATTGTAGCTGGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGCGCCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGGGAGGGGATGAGGAGGATAAAGAGGTGGGTCGTGAGGAGTCGGTTGAGGTTGATATGGGTTCAGGAAGGAATGCGGGCTCTGTGGGTGGAGATGGTGGTGAAGCGGGTTCGGGTGGGAAGGTAGAGTCGGTGGAAGAAAGAAGGGGAGTGGTTGCAGTGGAGGAGGTTGGGGTGGTGGTTAGGGAGGAGAGTTTGAAGGGAGCAGGGGCAGCTGCTGTTGCTGGTTCGGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGAGGACTTCTGGAGGAAGTTTTTGGGCGTGGGATTACGGATGGTGTGAAACAAGAGAAGAAGGTAGTTGATGGTGGGGCTGATTTGAAGGTACAGAAAGTTAGTGTTGCAGATGATGAAGTAAGGAATCCTGGAATTGGCACTGTAGCTGCAAGTTCTTCGGAAACAGTGGAGAATTCTTGTGTTTGGACCCGAGTTGTGGAAAAAGCTCCAGTTGTCAGTGACGAAGGTTCGAGTCCCAAACCCTTGAATGGACAAAGGCAAGTGTCTGCTGCTGCTGGTGATGTTTCAGGCACGGACAAAGGAGATACTATGACTTCCGAAGTCGCAGGTGTAGAGATTGGTAAAATTGATGAAAGTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGTCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATACAGCTGCTTCGTCCCTGCCAGCTAATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAGATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTACCAGCAAAACTACATCCTGGACGCATAGATCGAGAGGGTGTTTTGAATTTTGAAAGTCATCAGCACTTGAAACACGAAGAGATTGTAGACAAGAACATGGTCCATGATATGGCCCAGGTTGAATTAAATGGAGGGCAAGAAATGGAAGTGGACAGCCAAGTTAATGATGCTGAACATGGTGAGTTGGTTTCAGGAAATGATCAGAACTCAAAAATTGAGAAATCATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAATGTGACCACTGATCTTGCTCAGTCCAAGACCAATGTTGTAGATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTATTCAAGAAGCTACGCAGCCTGAAAGCTCTGAAATAGTGCCTCAACTTAGATATGAGCTGCCACAAAAAAATGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTGGCCTATTTTGGGGACCGAACATTTGCATGGAATGAAGTATCTAGTTTAAAGCCTTTCCGATCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGCCATGAATCGAGTAGAGTGGAAGGAGTAGATGAATCTGCAGGTGCAAGTTCATTTGAATCTAATAAACTACTTAAATATGTAAAAGCTTTAGCACGGTTCCCATCTGGTGGCAGTGACAAATTAGAACTTGTCATTGCCAAGGCTCATTTGCTGGCATTTTTCCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAAAGTAAAACAGATAATTCGCTCTCTGGGGACAAAACATGTCCAGGTGAAATTACTGAGCATGCGACTTATATTGGGAAGGATGATAAGAAGACTGGCCCGGAGATTGAGGAACTCAAGTCTAGCTCTTCTCATAAACGTAAACATAATCTAAGAGAAGGAGTGTATGCTAAAATGAAAGAGAGAAGCCTGTCAGAATTAATGGGTGGCGAAACAGGTTCTCCAGATGGTAATGATTGGTTGGATGTGAAGGCTTCGCCATTATCTGGCAAGAGACGGAAGGGTGCCGAGTATCTGGCTGCTGATTTAGCCACACAAGATGGGAGGAAAGCTGCTTCCCATTCAACAGTTTCAAATACAACACCTTCACCAAAACCATCGTTTAAGATTGGTGAATGTATCCAGAGAGCTGCAAGCCAACTGTCAGGGTCTACTATAGTGAAGAGCAGCACTGACAGACCTGCTGTGCAGGGATCTGATGTTCCCTTCCAGAGTTCCGATGATACTCTCAGAGGAGTGAATAATACAACGAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTCAGGAATTCTGTGGTTGTTGGCCAGAAATCTGGGGTGGTGCTTTCAGTTGTAAACAAAGTTGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGATATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAAGCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGGGCAACCAGAAAAGCCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCGGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTTGTTTTTAAGAGATGTTCTGATGCAGAAGTTGCTTGCAATAGTGCTGGGAAATTCAACATCTTTGGACAGATTACTGTAAATTACCAGCTCAACTATACTCCCTCTCAGCTCAACTACACGCCATCTATTACGTTTGGTGCATCTACCAGTGCTGCAGCCCAGGATCAGGAGGTGCAACTTGTTTTTCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAGCTTGATCTTTCAACCCATGACGAGATGCAACTAGACCTCTCAACATTCGAAGTTAATCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1199

Amino Acids

130.57

Weight (kDa)

5.0

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 520 - 602 4.2e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 770, 1934
Acc36I ACCTGC 5 cut(s) 770, 820, 889, 1934, 2590
AccB1I GGYRCC 1 cut(s) 2405
AccB7I CCANNNNNTGG 4 cut(s) 914, 1129, 2012, 2387
AccI GTMKAC 2 cut(s) 1107, 2552
AccIII TCCGGA 1 cut(s) 3061
AciI CCGC 2 cut(s) 236, 266
AclWI GGATC 5 cut(s) 930, 1009, 1275, 2602, 3405
AcoI YGGCCR 1 cut(s) 2779
AcsI RAATTY 8 cut(s) 625, 931, 1066, 2096, 2764, 2842, 2873, 3284
AcuI CTGAAG 4 cut(s) 1383, 2732, 3126, 3206
AfaI GTAC 5 cut(s) 154, 543, 1543, 2657, 2734
AfeI AGCGCT 1 cut(s) 3173
AflIII ACRYGT 3 cut(s) 975, 1816, 2159
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 1817
AjnI CCWGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 3023, 3393
AloI GAACNNNNNNTCC 2 cut(s) 2586, 2618
Alw21I GWGCWC 1 cut(s) 3134
Alw26I GTCTC 3 cut(s) 1558, 2389, 2858
AlwI GGATC 5 cut(s) 930, 1009, 1275, 2602, 3405
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 642
Aor13HI TCCGGA 1 cut(s) 3061
Aor51HI AGCGCT 1 cut(s) 3173
AoxI GGCC 6 cut(s) 1131, 1595, 1671, 2218, 2779, 3014
ApoI RAATTY 8 cut(s) 625, 931, 1066, 2096, 2764, 2842, 2873, 3284
ArsI GACNNNNNNTTYG 2 cut(s) 1676, 1708
AseI ATTAAT 1 cut(s) 2321
AspLEI GCGC 2 cut(s) 88, 3174
AspS9I GGNCC 6 cut(s) 639, 1120, 1132, 1684, 2219, 3179
AsuC2I CCSGG 1 cut(s) 2222
AsuHPI GGTGA 7 cut(s) 272, 743, 1211, 2180, 2481, 2525, 3139
AsuII TTCGAA 1 cut(s) 3582
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 4 cut(s) 639, 1120, 1684, 3179
BalI TGGCCA 1 cut(s) 2781
BanI GGYRCC 1 cut(s) 2405
BanII GRGCYC 2 cut(s) 242, 3134
BauI CACGAG 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 2219
Bbv12I GWGCWC 1 cut(s) 3134
BceAI ACGGC 1 cut(s) 3207
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 3025, 3395
BciVI GTATCC 1 cut(s) 2531
BclI TGATCA 2 cut(s) 1219, 3430
BcnI CCSGG 1 cut(s) 2222
BcoDI GTCTC 3 cut(s) 1558, 2389, 2858
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2666, 3228, 3566
BfmI CTRYAG 4 cut(s) 594, 1941, 2556, 3387
BfoI RGCGCY 1 cut(s) 3175
BfuAI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2590
BfuI GTATCC 1 cut(s) 2531
BmcAI AGTACT 2 cut(s) 2657, 2734
Bme1390I CCNGG 9 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 2222, 3025, 3395
Bme18I GGWCC 4 cut(s) 639, 1120, 1684, 3179
BmeT110I CYCGRG 1 cut(s) 642
BmgBI CACGTC 1 cut(s) 1817
BmgT120I GGNCC 6 cut(s) 639, 1120, 1132, 1684, 2219, 3179
BmiI GGNNCC 4 cut(s) 641, 1685, 2003, 2407
BmrFI CCNGG 9 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 2222, 3025, 3395
BmrI ACTGGG 1 cut(s) 1440
BmuI ACTGGG 1 cut(s) 1440
BpiI GAAGAC 1 cut(s) 2219
BplI GAGNNNNNCTC 4 cut(s) 909, 941, 3116, 3148
BpmI CTGGAG 3 cut(s) 482, 645, 2328
Bpu10I CCTNAGC 1 cut(s) 993
Bpu14I TTCGAA 1 cut(s) 3582
BpuEI CTTGAG 4 cut(s) 2222, 2749, 2985, 3024
BpuMI CCSGG 1 cut(s) 2222
BsaJI CCNNGG 6 cut(s) 1134, 1589, 1743, 2043, 2977, 3393
BsaWI WCCGGW 1 cut(s) 3061
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 2326, 2356
Bse1I ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3381
Bse3DI GCAATG 2 cut(s) 1644, 2037
BseAI TCCGGA 1 cut(s) 3061
BseBI CCWGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 3025, 3395
BseDI CCNNGG 6 cut(s) 1134, 1589, 1743, 2043, 2977, 3393
BseMI GCAATG 2 cut(s) 1644, 2037
BseMII CTCAG 9 cut(s) 1007, 1337, 1569, 1620, 2169, 2644, 2853, 3114, 3353
BseNI ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3381
BseRI GAGGAG 5 cut(s) 188, 212, 338, 362, 2649
BseYI CCCAGC 3 cut(s) 44, 1895, 3278
BsgI GTGCAG 2 cut(s) 867, 2609
BshFI GGCC 6 cut(s) 1133, 1597, 1673, 2220, 2781, 3016
BshNI GGYRCC 1 cut(s) 2405
BsiHKAI GWGCWC 1 cut(s) 3134
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 2 cut(s) 2222, 3062
BslFI GGGAC 4 cut(s) 672, 886, 1697, 2165
BsmAI GTCTC 3 cut(s) 1558, 2389, 2858
BsmBI CGTCTC 1 cut(s) 2389
BsmFI GGGAC 4 cut(s) 672, 886, 1697, 2165
BsmI GAATGC 2 cut(s) 238, 3097
BsnI GGCC 6 cut(s) 1133, 1597, 1673, 2220, 2781, 3016
BsoBI CYCGRG 1 cut(s) 642
Bsp119I TTCGAA 1 cut(s) 3582
Bsp1286I GDGCHC 2 cut(s) 242, 3134
Bsp13I TCCGGA 1 cut(s) 3061
Bsp19I CCATGG 1 cut(s) 1589
BspACI CCGC 2 cut(s) 236, 266
BspANI GGCC 6 cut(s) 1133, 1597, 1673, 2220, 2781, 3016
BspCNI CTCAG 9 cut(s) 1006, 1336, 1568, 1621, 2170, 2643, 2854, 3115, 3352
BspEI TCCGGA 1 cut(s) 3061
BspHI TCATGA 2 cut(s) 982, 3433
BspLI GGNNCC 4 cut(s) 641, 1685, 2003, 2407
BspMAI CTGCAG 2 cut(s) 1945, 3391
BspMI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2590
BspPI GGATC 5 cut(s) 930, 1009, 1275, 2602, 3405
BspQI GCTCTTC 4 cut(s) 1574, 2254, 2558, 2925
BspT104I TTCGAA 1 cut(s) 3582
BspT107I GGYRCC 1 cut(s) 2405
BsrDI GCAATG 2 cut(s) 1644, 2037
BsrI ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3381
BssECI CCNNGG 6 cut(s) 1134, 1589, 1743, 2043, 2977, 3393
BssSI CACGAG 1 cut(s) 30
BssT1I CCWWGG 3 cut(s) 1589, 2043, 2977
Bst2BI CACGAG 1 cut(s) 30
Bst2UI CCWGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 3025, 3395
Bst4CI ACNGT 7 cut(s) 595, 619, 1661, 2001, 2470, 2544, 3310
Bst6I CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2558, 2925
BstAPI GCANNNNNTGC 1 cut(s) 3263
BstBI TTCGAA 1 cut(s) 3582
BstC8I GCNNGC 8 cut(s) 238, 909, 2058, 2184, 2536, 2937, 3014, 3268
BstDSI CCRYGG 1 cut(s) 1589
BstEII GGTNACC 1 cut(s) 2856
BstH2I RGCGCY 1 cut(s) 3175
BstHHI GCGC 2 cut(s) 88, 3174
BstMAI GTCTC 3 cut(s) 1558, 2389, 2858
BstMWI GCNNNNNNNGC 6 cut(s) 371, 380, 389, 890, 2429, 3263
BstNI CCWGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 3025, 3395
BstNSI RCATGY 4 cut(s) 979, 2163, 2186, 3532
BstPI GGTNACC 1 cut(s) 2856
BstSCI CCNGG 9 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 2220, 3023, 3393
BstSFI CTRYAG 4 cut(s) 594, 1941, 2556, 3387
BstV2I GAAGAC 1 cut(s) 2219
BstX2I RGATCY 2 cut(s) 922, 2594
BstXI CCANNNNNNTGG 3 cut(s) 2108, 2789, 2924
BstYI RGATCY 2 cut(s) 922, 2594
BsuI GTATCC 1 cut(s) 2531
BsuRI GGCC 6 cut(s) 1133, 1597, 1673, 2220, 2781, 3016
BtgI CCRYGG 1 cut(s) 1589
BtrI CACGTC 1 cut(s) 1817
BtsI GCAGTG 3 cut(s) 324, 1763, 2023
BveI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2590
Cac8I GCNNGC 8 cut(s) 238, 909, 2058, 2184, 2536, 2937, 3014, 3268
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 2 cut(s) 982, 3433
CfoI GCGC 2 cut(s) 88, 3174
Cfr13I GGNCC 6 cut(s) 639, 1120, 1132, 1684, 2219, 3179
CseI GACGC 1 cut(s) 1050
Csp6I GTAC 5 cut(s) 153, 542, 1542, 2656, 2733
CspCI CAANNNNNGTGG 4 cut(s) 1300, 1335, 2803, 2838
CviQI GTAC 5 cut(s) 153, 542, 1542, 2656, 2733
DraI TTTAAA 3 cut(s) 1296, 1719, 2073
EaeI YGGCCR 1 cut(s) 2779
Eam1104I CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2558, 2925
EarI CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2558, 2925
Ecl136II GAGCTC 1 cut(s) 3132
Eco130I CCWWGG 3 cut(s) 1589, 2043, 2977
Eco24I GRGCYC 2 cut(s) 242, 3134
Eco47I GGWCC 4 cut(s) 639, 1120, 1684, 3179
Eco47III AGCGCT 1 cut(s) 3173
Eco53kI GAGCTC 1 cut(s) 3132
Eco57I CTGAAG 4 cut(s) 1383, 2732, 3126, 3206
Eco88I CYCGRG 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 2856
EcoICRI GAGCTC 1 cut(s) 3132
EcoO65I GGTNACC 1 cut(s) 2856
EcoRI GAATTC 4 cut(s) 625, 931, 2096, 2764
EcoRII CCWGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 3023, 3393
EcoT14I CCWWGG 3 cut(s) 1589, 2043, 2977
EcoT22I ATGCAT 3 cut(s) 1250, 1847, 3444
EcoT38I GRGCYC 2 cut(s) 242, 3134
ErhI CCWWGG 3 cut(s) 1589, 2043, 2977
Esp3I CGTCTC 1 cut(s) 2389
FaqI GGGAC 4 cut(s) 672, 886, 1697, 2165
FauI CCCGC 2 cut(s) 229, 259
FbaI TGATCA 2 cut(s) 1219, 3430
FblI GTMKAC 2 cut(s) 1107, 2552
FriOI GRGCYC 2 cut(s) 242, 3134
FspBI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2666, 3228, 3566
GlaI GCGC 2 cut(s) 87, 3173
GsaI CCCAGC 3 cut(s) 48, 1899, 3282
GsuI CTGGAG 3 cut(s) 482, 645, 2328
HaeII RGCGCY 1 cut(s) 3175
HaeIII GGCC 6 cut(s) 1133, 1597, 1673, 2220, 2781, 3016
HapII CCGG 2 cut(s) 2222, 3062
HgaI GACGC 1 cut(s) 1050
HhaI GCGC 2 cut(s) 88, 3174
Hin6I GCGC 2 cut(s) 86, 3172
HinP1I GCGC 2 cut(s) 86, 3172
HindIII AAGCTT 1 cut(s) 1989
HpaII CCGG 2 cut(s) 2222, 3062
HphI GGTGA 7 cut(s) 272, 743, 1211, 2180, 2481, 2525, 3139
Hpy166II GTNNAC 6 cut(s) 1108, 1171, 1411, 2264, 2553, 2812
Hpy8I GTNNAC 6 cut(s) 1108, 1171, 1411, 2264, 2553, 2812
HpyCH4III ACNGT 7 cut(s) 595, 619, 1661, 2001, 2470, 2544, 3310
HpyCH4IV ACGT 3 cut(s) 1816, 2260, 3365
HpyF10VI GCNNNNNNNGC 6 cut(s) 371, 380, 389, 890, 2429, 3263
HpySE526I ACGT 3 cut(s) 1816, 2260, 3365
HspAI GCGC 2 cut(s) 86, 3172
Kpn2I TCCGGA 1 cut(s) 3061
Ksp22I TGATCA 2 cut(s) 1219, 3430
LguI GCTCTTC 4 cut(s) 1574, 2254, 2558, 2925
LmnI GCTCC 2 cut(s) 362, 664
MaeI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2666, 3228, 3566
MaeII ACGT 3 cut(s) 1816, 2260, 3365
MaeIII GTNAC 6 cut(s) 671, 1306, 1357, 2018, 2679, 2856
MfeI CAATTG 1 cut(s) 1789
MflI RGATCY 2 cut(s) 922, 2594
MhlI GDGCHC 2 cut(s) 242, 3134
MlsI TGGCCA 1 cut(s) 2781
MluNI TGGCCA 1 cut(s) 2781
MlyI GAGTC 6 cut(s) 67, 209, 296, 694, 851, 3055
MmeI TCCRAC 3 cut(s) 979, 2100, 2343
Mox20I TGGCCA 1 cut(s) 2781
Mph1103I ATGCAT 3 cut(s) 1250, 1847, 3444
MroI TCCGGA 1 cut(s) 3061
MscI TGGCCA 1 cut(s) 2781
MslI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3432
Msp20I TGGCCA 1 cut(s) 2781
MspA1I CMGCKG 2 cut(s) 374, 893
MspI CCGG 2 cut(s) 2222, 3062
MspR9I CCNGG 9 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 2222, 3025, 3395
MssI GTTTAAAC 1 cut(s) 1296
MunI CAATTG 1 cut(s) 1789
Mva1269I GAATGC 2 cut(s) 238, 3097
MvaI CCWGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 3025, 3395
MwoI GCNNNNNNNGC 6 cut(s) 371, 380, 389, 890, 2429, 3263
NciI CCSGG 1 cut(s) 2222
NcoI CCATGG 1 cut(s) 1589
NlaIV GGNNCC 4 cut(s) 641, 1685, 2003, 2407
NmeAIII GCCGAG 1 cut(s) 2434
NmuCI GTSAC 4 cut(s) 671, 1306, 2018, 2679
NsiI ATGCAT 3 cut(s) 1250, 1847, 3444
NspI RCATGY 4 cut(s) 979, 2163, 2186, 3532
NspV TTCGAA 1 cut(s) 3582
PaeI GCATGC 1 cut(s) 2186
PagI TCATGA 2 cut(s) 982, 3433
PaqCI CACCTGC 2 cut(s) 770, 1934
PciI ACATGT 2 cut(s) 975, 2159
PciSI GCTCTTC 4 cut(s) 1574, 2254, 2558, 2925
PcsI WCGNNNNNNNCGW 1 cut(s) 1048
PctI GAATGC 2 cut(s) 238, 3097
PfeI GAWTC 5 cut(s) 577, 1875, 1910, 1937, 1961
PflMI CCANNNNNTGG 4 cut(s) 914, 1129, 2012, 2387
PfoI TCCNGGA 2 cut(s) 580, 1036
PleI GAGTC 6 cut(s) 66, 208, 295, 693, 850, 3054
PmeI GTTTAAAC 1 cut(s) 1296
PpsI GAGTC 6 cut(s) 66, 208, 295, 693, 850, 3054
PscI ACATGT 2 cut(s) 975, 2159
PshBI ATTAAT 1 cut(s) 2321
Psp124BI GAGCTC 1 cut(s) 3134
Psp6I CCWGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 3023, 3393
PspEI GGTNACC 1 cut(s) 2856
PspFI CCCAGC 3 cut(s) 44, 1895, 3278
PspGI CCWGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 3023, 3393
PspN4I GGNNCC 4 cut(s) 641, 1685, 2003, 2407
PspPI GGNCC 6 cut(s) 639, 1120, 1132, 1684, 2219, 3179
PstI CTGCAG 2 cut(s) 1945, 3391
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 922, 2594
PvuII CAGCTG 2 cut(s) 374, 893
RsaI GTAC 5 cut(s) 154, 543, 1543, 2657, 2734
RsaNI GTAC 5 cut(s) 153, 542, 1542, 2656, 2733
RseI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3432
SacI GAGCTC 1 cut(s) 3134
SapI GCTCTTC 4 cut(s) 1574, 2254, 2558, 2925
Sau96I GGNCC 6 cut(s) 639, 1120, 1132, 1684, 2219, 3179
ScaI AGTACT 2 cut(s) 2657, 2734
SchI GAGTC 6 cut(s) 67, 209, 296, 694, 851, 3055
ScrFI CCNGG 9 cut(s) 582, 1038, 1136, 1599, 1745, 2166, 2222, 3025, 3395
SduI GDGCHC 2 cut(s) 242, 3134
SfcI CTRYAG 4 cut(s) 594, 1941, 2556, 3387
SfuI TTCGAA 1 cut(s) 3582
SinI GGWCC 4 cut(s) 639, 1120, 1684, 3179
SmiMI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3432
SmlI CTYRAG 4 cut(s) 2237, 2728, 3000, 3039
SmoI CTYRAG 4 cut(s) 2237, 2728, 3000, 3039
SpeI ACTAGT 1 cut(s) 155
SphI GCATGC 1 cut(s) 2186
SsiI CCGC 2 cut(s) 236, 266
SspMI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2666, 3228, 3566
SstI GAGCTC 1 cut(s) 3134
StyD4I CCNGG 9 cut(s) 580, 1036, 1134, 1597, 1743, 2164, 2220, 3023, 3393
StyI CCWWGG 3 cut(s) 1589, 2043, 2977
TaaI ACNGT 7 cut(s) 595, 619, 1661, 2001, 2470, 2544, 3310
TaiI ACGT 3 cut(s) 1819, 2263, 3368
TaqI TCGA 4 cut(s) 683, 1051, 1913, 3582
TaqII GACCGA 2 cut(s) 1701, 2679
TatI WGTACW 3 cut(s) 152, 2655, 2732
TfiI GAWTC 5 cut(s) 577, 1875, 1910, 1937, 1961
TseFI GTSAC 4 cut(s) 671, 1306, 2018, 2679
Tsp45I GTSAC 4 cut(s) 671, 1306, 2018, 2679
TspGWI ACGGA 5 cut(s) 503, 761, 2057, 2413, 2959
Van91I CCANNNNNTGG 4 cut(s) 914, 1129, 2012, 2387
VpaK11BI GGWCC 4 cut(s) 639, 1120, 1684, 3179
VspI ATTAAT 1 cut(s) 2321
XapI RAATTY 8 cut(s) 625, 931, 1066, 2096, 2764, 2842, 2873, 3284
XbaI TCTAGA 1 cut(s) 2665
XceI RCATGY 4 cut(s) 979, 2163, 2186, 3532
XmiI GTMKAC 2 cut(s) 1107, 2552
XspI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2666, 3228, 3566
ZrmI AGTACT 2 cut(s) 2657, 2734
Zsp2I ATGCAT 3 cut(s) 1250, 1847, 3444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.