RchiOBHm_Chr1g0380251

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005995
Physical Location & Seq
Reverse (-)
65964686 .. 65969120
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGATATGCAAGAGTGGGATAAGGAATCTGATTGTTCAAGCTGGGAATATAGTAGCGCGCACAAAAACTGTGGCGCATTTGAACAACATTGGGAGAGTTTGCATGCAAACCTCTTTGGGGAACCGCGAATTTAAGACAACAAGTTTAAGGATGATGGTTGATTCTAGTGCGACCGAGAAACAGGGTTCCATTGTTGATGTCTTATTGCAGAAAGATGACGAGGGTGGATTTCTCAGTGGAGGCTGGAAAAGTGAAGGCGGAAAACTTAGCTGTGGCTATTGCAGTTTTAGGGGGAAAAGAGCCACCATGGAGGATTTTTATGATATAAAAACTTCCAAGATTGATGGGCAAACAGTTTGCCTCTTTGGAATTTTTGATGGTCATGGTGGTTCTCGTGCTGCTGAATATCTGAAGGAGCATCTATTTGAGAATCTTATGAAGCATCCAAACTTTATCACAGACACCAAGTTAGCTATTAGTGAATCATATCAACAAACTGATGCAGCCTTTTTGGATTCTGAAAGGGATACATACCGCGATGATGGTTCTACTGCTTCAACAGCAGTCTTAGTGGGCAACCATCTATACGTTGCCAATGTTGGAGATTCTCGTACAGTAATATCAAAGGAAGGCAAAGCAATTCCTCTATCTGAGGATCATAAACCAAATCGAACTGATGAGAGAAAGAGAATTGAAAATGCTGGGGGCATTGTAATGTGGGCAGGAACTTGGAGGGTAGGAGGTGTTTTGGCTATGTCTCGGGCTTTCGGAAACCGCATGTTGAAGCAATTTGTTGTCGCAGAACCTGAGATCCAGGACCAAGTGATAGATGAAGAATTTGAATTGCTGGTGCTCGCTAGTGATGGGCTGTGGGATGTGGTGCCAAATGAGGATGCTGTTTCACTTGCCCGAACAGAAGAAGAACCTGAGGCGGCAGCTCGGAAGTTAACGGGAACAGCATTTGCCCGTGGTAGTGCAGACAATATAACATCCATTGTGGTGAGGTTCCACCATGACAAAGCAGAGTCTGCTGGGGCTAACCATGATTAG

Protein Analysis

349

Amino Acids

38.23

Weight (kDa)

5.57

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 329 3.4e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 3 cut(s) 58, 126, 537
AciI CCGC 5 cut(s) 124, 258, 535, 775, 932
AclWI GGATC 2 cut(s) 663, 805
AcsI RAATTY 3 cut(s) 128, 369, 836
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 1 cut(s) 613
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 6 cut(s) 38, 82, 558, 695, 784, 842
AjnI CCWGG 1 cut(s) 813
AloI GAACNNNNNNTCC 2 cut(s) 795, 827
AluBI AGCT 4 cut(s) 41, 270, 473, 938
AluI AGCT 4 cut(s) 41, 270, 473, 938
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 1 cut(s) 762
AlwI GGATC 2 cut(s) 663, 805
AlwNI CAGNNNCTG 2 cut(s) 806, 1028
Ama87I CYCGRG 1 cut(s) 759
ApeKI GCWGC 3 cut(s) 398, 503, 935
ApoI RAATTY 3 cut(s) 128, 369, 836
AspLEI GCGC 3 cut(s) 58, 60, 76
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 1 cut(s) 1012
AvaI CYCGRG 1 cut(s) 759
AvaII GGWCC 1 cut(s) 817
AxyI CCTNAGG 1 cut(s) 927
BanI GGYRCC 1 cut(s) 880
BauI CACGAG 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 855
BbvI GCAGC 3 cut(s) 385, 515, 947
BccI CCATC 6 cut(s) 148, 338, 371, 536, 588, 857
BciT130I CCWGG 1 cut(s) 815
BciVI GTATCC 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 2 cut(s) 165, 858
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 4 cut(s) 399, 504, 933, 936
BlsI GCNGC 4 cut(s) 400, 505, 934, 937
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 759
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 4 cut(s) 122, 187, 882, 1007
BmrFI CCNGG 1 cut(s) 815
BmsI GCATC 4 cut(s) 427, 451, 490, 883
BsaJI CCNNGG 2 cut(s) 306, 967
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse21I CCTNAGG 1 cut(s) 927
BseBI CCWGG 1 cut(s) 815
BseDI CCNNGG 2 cut(s) 306, 967
BseGI GGATG 5 cut(s) 156, 442, 880, 898, 989
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMII CTCAG 4 cut(s) 247, 642, 798, 918
BsePI GCGCGC 1 cut(s) 56
BseXI GCAGC 3 cut(s) 385, 515, 947
BseYI CCCAGC 3 cut(s) 41, 701, 1031
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 3 cut(s) 58, 126, 537
Bsh1285I CGRYCG 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 855
BsiHKCI CYCGRG 1 cut(s) 759
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 759
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 2 cut(s) 655, 810
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 5 cut(s) 124, 258, 535, 775, 932
BspCNI CTCAG 4 cut(s) 246, 643, 799, 919
BspFNI CGCG 3 cut(s) 58, 126, 537
BspLI GGNNCC 4 cut(s) 122, 187, 882, 1007
BspPI GGATC 2 cut(s) 663, 805
BspT107I GGYRCC 1 cut(s) 880
BssECI CCNNGG 2 cut(s) 306, 967
BssHII GCGCGC 1 cut(s) 56
BssMI GATC 2 cut(s) 655, 810
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 3 cut(s) 70, 355, 616
BstAPI GCANNNNNTGC 1 cut(s) 1028
BstC8I GCNNGC 3 cut(s) 58, 104, 855
BstDEI CTNAG 6 cut(s) 233, 266, 568, 651, 807, 927
BstDSI CCRYGG 2 cut(s) 306, 967
BstF5I GGATG 5 cut(s) 156, 442, 880, 898, 989
BstFNI CGCG 3 cut(s) 58, 126, 537
BstHHI GCGC 3 cut(s) 58, 60, 76
BstKTI GATC 2 cut(s) 658, 813
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 2 cut(s) 655, 810
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 560, 1028
BstNI CCWGG 1 cut(s) 815
BstNSI RCATGY 2 cut(s) 106, 781
BstSCI CCNGG 1 cut(s) 813
BstUI CGCG 3 cut(s) 58, 126, 537
BstV1I GCAGC 3 cut(s) 385, 515, 947
BstX2I RGATCY 1 cut(s) 810
BstYI RGATCY 1 cut(s) 810
Bsu36I CCTNAGG 1 cut(s) 927
BsuI GTATCC 1 cut(s) 520
BtgI CCRYGG 2 cut(s) 306, 967
BtgZI GCGATG 1 cut(s) 552
BtsCI GGATG 5 cut(s) 156, 442, 880, 898, 989
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 3 cut(s) 58, 104, 855
CaiI CAGNNNCTG 2 cut(s) 806, 1028
CfoI GCGC 3 cut(s) 58, 60, 76
Cfr13I GGNCC 1 cut(s) 817
Csp6I GTAC 1 cut(s) 612
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 6 cut(s) 103, 307, 383, 778, 1013, 1043
CviQI GTAC 1 cut(s) 612
DdeI CTNAG 6 cut(s) 233, 266, 568, 651, 807, 927
DpnI GATC 2 cut(s) 657, 812
DpnII GATC 2 cut(s) 655, 810
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 927
Eco88I CYCGRG 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 813
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 6 cut(s) 106, 310, 386, 781, 1016, 1046
FatI CATG 6 cut(s) 102, 306, 382, 777, 1012, 1042
Fnu4HI GCNGC 4 cut(s) 399, 504, 933, 936
FokI GGATG 5 cut(s) 163, 429, 887, 905, 976
Fsp4HI GCNGC 4 cut(s) 399, 504, 933, 936
FspBI CTAG 2 cut(s) 165, 858
GlaI GCGC 3 cut(s) 57, 59, 75
GluI GCNGC 4 cut(s) 399, 504, 933, 936
GsaI CCCAGC 3 cut(s) 45, 705, 1035
HhaI GCGC 3 cut(s) 58, 60, 76
Hin1II CATG 6 cut(s) 106, 310, 386, 781, 1016, 1046
Hin6I GCGC 3 cut(s) 56, 58, 74
HinP1I GCGC 3 cut(s) 56, 58, 74
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HinfI GANTC 7 cut(s) 25, 161, 430, 482, 515, 605, 1025
HpaI GTTAAC 1 cut(s) 948
HphI GGTGA 1 cut(s) 1012
Hpy166II GTNNAC 1 cut(s) 948
Hpy188I TCNGA 6 cut(s) 30, 411, 520, 652, 770, 942
Hpy8I GTNNAC 1 cut(s) 948
HpyAV CCTTC 3 cut(s) 248, 406, 623
HpyCH4III ACNGT 3 cut(s) 70, 355, 616
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 7 cut(s) 9, 102, 106, 208, 282, 503, 977
HpyF10VI GCNNNNNNNGC 2 cut(s) 560, 1028
HpyF3I CTNAG 6 cut(s) 233, 266, 568, 651, 807, 927
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 6 cut(s) 106, 310, 386, 781, 1016, 1046
HspAI GCGC 3 cut(s) 56, 58, 74
KspAI GTTAAC 1 cut(s) 948
Kzo9I GATC 2 cut(s) 655, 810
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 3 cut(s) 385, 515, 947
LweI GCATC 4 cut(s) 427, 451, 490, 883
MaeI CTAG 2 cut(s) 165, 858
MaeII ACGT 1 cut(s) 588
MalI GATC 2 cut(s) 657, 812
MboI GATC 2 cut(s) 655, 810
MboII GAAGA 3 cut(s) 845, 929, 932
MflI RGATCY 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 855
MluCI AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
MlyI GAGTC 1 cut(s) 1034
MmeI TCCRAC 1 cut(s) 580
MseI TTAA 3 cut(s) 132, 146, 947
MslI CAYNNNNRTG 2 cut(s) 713, 998
MspR9I CCNGG 1 cut(s) 815
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 3 cut(s) 58, 126, 537
MwoI GCNNNNNNNGC 2 cut(s) 560, 1028
NcoI CCATGG 1 cut(s) 306
NdeII GATC 2 cut(s) 655, 810
NlaIII CATG 6 cut(s) 106, 310, 386, 781, 1016, 1046
NlaIV GGNNCC 4 cut(s) 122, 187, 882, 1007
NspI RCATGY 2 cut(s) 106, 781
PaeI GCATGC 1 cut(s) 106
PauI GCGCGC 1 cut(s) 56
PfeI GAWTC 6 cut(s) 25, 161, 430, 482, 515, 605
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 4 cut(s) 400, 505, 934, 937
PleI GAGTC 1 cut(s) 1033
PpsI GAGTC 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 813
PspFI CCCAGC 3 cut(s) 41, 701, 1031
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 4 cut(s) 122, 187, 882, 1007
PspPI GGNCC 1 cut(s) 817
PstNI CAGNNNCTG 2 cut(s) 806, 1028
PsuI RGATCY 1 cut(s) 810
PteI GCGCGC 1 cut(s) 56
RsaI GTAC 1 cut(s) 613
RsaNI GTAC 1 cut(s) 612
RseI CAYNNNNRTG 2 cut(s) 713, 998
SaqAI TTAA 3 cut(s) 132, 146, 947
SatI GCNGC 4 cut(s) 399, 504, 933, 936
Sau3AI GATC 2 cut(s) 655, 810
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 1 cut(s) 815
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 4 cut(s) 427, 451, 490, 883
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 713, 998
SphI GCATGC 1 cut(s) 106
Sse9I AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
SsiI CCGC 5 cut(s) 124, 258, 535, 775, 932
SspMI CTAG 2 cut(s) 165, 858
StyD4I CCNGG 1 cut(s) 813
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 3 cut(s) 70, 355, 616
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 670
TaqII GACCGA 1 cut(s) 188
TasI AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
TauI GCSGC 1 cut(s) 935
TfiI GAWTC 6 cut(s) 25, 161, 430, 482, 515, 605
Tru1I TTAA 3 cut(s) 132, 146, 947
Tru9I TTAA 3 cut(s) 132, 146, 947
TscAI CASTG 1 cut(s) 241
TseI GCWGC 3 cut(s) 398, 503, 935
TspDTI ATGAA 2 cut(s) 452, 846
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 3 cut(s) 128, 369, 836
XceI RCATGY 2 cut(s) 106, 781
XspI CTAG 2 cut(s) 165, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.