RCHIOBHM_CPG0502121

Produces ATP from ADP in the presence of a proton gradient across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0005978
Physical Location & Seq
Forward (+)
56986 .. 58717
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15700

Sequence Viewer

Length: 1479 bp
ATGAGAATAAATCCTACTACTTCTGGTCCTGGGGTTTCCGCACTTGAAAAAAAAAACCTGGGGCGTATTGCTCAAATCATTGGTCCGGTACTGGATGTAGCTTTTCCACCCGGCAAGATGCCTAATATTTACAACGCTCTGGTAGTTAAGGGTCGAGATACCGCTGGTCAACAAATTAATGTGACTTGTGAAGTACAGCAATTATTAGGAAATAATCGAGTTAGAGCTGTAGCTATGAGTGCTACAGATGGTCTAACGAGAGGAATGGAAGTGATTGACACAGGAGCTCCTTTAAGTGTTCCAGTCGGCGGAGCGACTCTAGGACGAATTTTTAACGTACTTGGAGAGCCTGTGGATAATTTAGGTCCTGTAGATACTCGTACAACATCTCCTATTCATAGATCTGCGCCTGCCTTTATCCAGTTAGATACAAAACTATCCATTTTTGAAACAGGAATTAAAGTTGTAGATCTTCTAGCCCCTTATCGTCGTGGAGGAAAAATCGGACTTTTTGGGGGAGCTGGAGTGGGTAAAACAGTACTCATTATGGAATTGATCAACAACATTGCCAAAGCTCATGGGGGTGTATCCGTATTTGGCGGAGTGGGTGAACGTACTCGTGAAGGAAATGATCTTTACATGGAAATGAAAGAATCCGGCGTAATTAATGAACAAAATATTCCCGAATCAAAAGTGGCTCTAGTCTATGGTCAAATGAATGAACCGCCCGGAGCTCGTATGAGGGTTGGTTTAACCGCCCTAACTATGGCGGAATATTTCCGAGATGTTAATGAACAAGACGTACTTCTATTTATCGACAATATCTTCCGTTTTGTCCAAGCAGGATCCGAAGTATCCGCTTTATTGGGCAGAATGCCTTCCGCTGTGGGTTATCAACCCACCCTTAGTACCGAAATGGGCTCTTTACAAGAAAGAATTACTTCTACCAAAGAGGGGTCCATAACTTCTATTCAAGCAGTTTATGTACCTGCGGACGATTTGACCGATCCTGCTCCGGCCACGACATTTGCACATTTAGATGCTACTACCGTACTATCAAGAGGATTAGCTGCCAAAGGTATCTATCCAGCAGTAGATCCTTTAGATTCAACATCAACTATGCTCCAACCTCGGATTGTTGGTGAAGAACATTATGAAACTGCGCAAAGAGTTAAAGAAACTTTACAACGTTACAAAGAACTTCAGGATATTATAGCTATCCTTGGGTTGGACGAATTATCCGAAGACGATCGCTTAACCGTAGCAAGAGCACGAAAAATTGAGCGTTTCTTATCACAACCTTTTTTCGTAGCAGAAGTATTTACCGGTTCCCCAGGAAAATATGTTGGTCTAGCAGAAACAATTAGAGGGTTTAAATTGATCCTTTCTGGAGAATTAGATGGTCTTCCTGAGCAGGCCTTTTATTTAGTAGGTAACATCGATGAAGCTACTGCGAAAGCTACGACCTTAGGAACTTAG

Protein Analysis

492

Amino Acids

52.78

Weight (kDa)

5.26

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 23 - 95 2.4e-20 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 152 - 372 3.6e-71 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 378 - 457 2.7e-16 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1164
Acc36I ACCTGC 1 cut(s) 997
AclI AACGTT 1 cut(s) 1189
AclWI GGATC 5 cut(s) 840, 853, 1001, 1091, 1375
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 1 cut(s) 327
AcuI CTGAAG 1 cut(s) 1187
AfiI CCNNNNNNNGG 4 cut(s) 308, 766, 954, 1228
AgeI ACCGGT 1 cut(s) 1325
AgsI TTSAA 4 cut(s) 47, 449, 974, 1110
AjnI CCWGG 3 cut(s) 28, 57, 1333
Alw21I GWGCWC 3 cut(s) 289, 736, 1273
AlwI GGATC 5 cut(s) 840, 853, 1001, 1091, 1375
AoxI GGCC 2 cut(s) 1017, 1416
ApeKI GCWGC 1 cut(s) 1070
ApoI RAATTY 1 cut(s) 327
ArsI GACNNNNNNTTYG 2 cut(s) 67, 99
AseI ATTAAT 2 cut(s) 177, 666
AsiGI ACCGGT 1 cut(s) 1325
Asp700I GAANNNNTTC 2 cut(s) 877, 940
AspLEI GCGC 2 cut(s) 409, 1165
AspS9I GGNCC 4 cut(s) 26, 83, 365, 957
AsuC2I CCSGG 2 cut(s) 111, 729
AsuHPI GGTGA 2 cut(s) 620, 1154
AvaII GGWCC 4 cut(s) 26, 83, 365, 957
AxyI CCTNAGG 1 cut(s) 1468
BamHI GGATCC 1 cut(s) 845
BanII GRGCYC 3 cut(s) 289, 736, 923
BauI CACGAG 1 cut(s) 618
BbsI GAAGAC 2 cut(s) 1251, 1397
Bbv12I GWGCWC 3 cut(s) 289, 736, 1273
BbvI GCAGC 1 cut(s) 1057
BccI CCATC 2 cut(s) 242, 1394
BciT130I CCWGG 3 cut(s) 30, 59, 1335
BciVI GTATCC 2 cut(s) 598, 865
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 2 cut(s) 111, 729
BfaI CTAG 4 cut(s) 320, 476, 701, 1352
BfmI CTRYAG 3 cut(s) 228, 243, 369
BfuAI ACCTGC 1 cut(s) 997
BfuI GTATCC 2 cut(s) 598, 865
BglII AGATCT 2 cut(s) 401, 469
BisI GCNGC 1 cut(s) 1071
BlsI GCNGC 1 cut(s) 1072
BmcAI AGTACT 1 cut(s) 540
Bme1390I CCNGG 5 cut(s) 30, 59, 111, 729, 1335
Bme18I GGWCC 4 cut(s) 26, 83, 365, 957
BmgT120I GGNCC 4 cut(s) 26, 83, 365, 957
BmiI GGNNCC 3 cut(s) 847, 958, 1330
BmrFI CCNGG 5 cut(s) 30, 59, 111, 729, 1335
BmsI GCATC 2 cut(s) 108, 1030
BpiI GAAGAC 2 cut(s) 1251, 1397
BpmI CTGGAG 2 cut(s) 543, 1410
Bpu10I CCTNAGC 1 cut(s) 1410
BpuMI CCSGG 2 cut(s) 111, 729
Bsa29I ATCGAT 1 cut(s) 1440
BsaJI CCNNGG 5 cut(s) 29, 58, 1130, 1222, 1333
BsaWI WCCGGW 2 cut(s) 85, 1325
BsaXI ACNNNNNCTCC 8 cut(s) 271, 301, 336, 366, 373, 403, 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 766, 954, 1228
Bse118I RCCGGY 1 cut(s) 1325
Bse1I ACTGG 3 cut(s) 96, 302, 421
Bse21I CCTNAGG 1 cut(s) 1468
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 3 cut(s) 30, 59, 1335
BseCI ATCGAT 1 cut(s) 1440
BseDI CCNNGG 5 cut(s) 29, 58, 1130, 1222, 1333
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 4 cut(s) 308, 766, 954, 1228
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 1401
BseNI ACTGG 3 cut(s) 96, 302, 421
BseXI GCAGC 1 cut(s) 1057
Bsh1285I CGRYCG 1 cut(s) 1252
BshFI GGCC 2 cut(s) 1019, 1418
BshTI ACCGGT 1 cut(s) 1325
BshVI ATCGAT 1 cut(s) 1440
BsiEI CGRYCG 1 cut(s) 1252
BsiHKAI GWGCWC 3 cut(s) 289, 736, 1273
BsiSI CCGG 6 cut(s) 86, 111, 657, 729, 1016, 1326
BslI CCNNNNNNNGG 4 cut(s) 308, 766, 954, 1228
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 2 cut(s) 1019, 1418
Bsp1286I GDGCHC 4 cut(s) 289, 736, 923, 1273
Bsp143I GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
BspANI GGCC 2 cut(s) 1019, 1418
BspCNI CTCAG 1 cut(s) 1402
BspDI ATCGAT 1 cut(s) 1440
BspLI GGNNCC 3 cut(s) 847, 958, 1330
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 5 cut(s) 840, 853, 1001, 1091, 1375
BsrDI GCAATG 1 cut(s) 564
BsrFI RCCGGY 1 cut(s) 1325
BsrI ACTGG 3 cut(s) 96, 302, 421
BssAI RCCGGY 1 cut(s) 1325
BssECI CCNNGG 5 cut(s) 29, 58, 1130, 1222, 1333
BssMI GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 1222
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 30, 59, 1335
Bst4CI ACNGT 3 cut(s) 538, 1051, 1261
BstC8I GCNNGC 2 cut(s) 411, 1416
BstDEI CTNAG 4 cut(s) 905, 1410, 1468, 1476
BstF5I GGATG 1 cut(s) 100
BstHHI GCGC 2 cut(s) 409, 1165
BstKTI GATC 9 cut(s) 404, 472, 558, 634, 848, 1009, 1099, 1252, 1383
BstMBI GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
BstMCI CGRYCG 1 cut(s) 1252
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 3 cut(s) 30, 59, 1335
BstSCI CCNGG 5 cut(s) 28, 57, 109, 727, 1333
BstSFI CTRYAG 3 cut(s) 228, 243, 369
BstV1I GCAGC 1 cut(s) 1057
BstV2I GAAGAC 2 cut(s) 1251, 1397
BstX2I RGATCY 4 cut(s) 401, 469, 845, 1096
BstYI RGATCY 4 cut(s) 401, 469, 845, 1096
Bsu15I ATCGAT 1 cut(s) 1440
Bsu36I CCTNAGG 1 cut(s) 1468
BsuI GTATCC 2 cut(s) 598, 865
BsuRI GGCC 2 cut(s) 1019, 1418
BsuTUI ATCGAT 1 cut(s) 1440
BtsCI GGATG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 2 cut(s) 411, 1416
CfoI GCGC 2 cut(s) 409, 1165
Cfr10I RCCGGY 1 cut(s) 1325
Cfr13I GGNCC 4 cut(s) 26, 83, 365, 957
ClaI ATCGAT 1 cut(s) 1440
CspAI ACCGGT 1 cut(s) 1325
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviAII CATG 2 cut(s) 578, 640
DdeI CTNAG 4 cut(s) 905, 1410, 1468, 1476
DpnI GATC 9 cut(s) 403, 471, 557, 633, 847, 1008, 1098, 1251, 1382
DpnII GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
DraI TTTAAA 1 cut(s) 1375
EaeI YGGCCR 1 cut(s) 1017
EciI GGCGGA 3 cut(s) 324, 615, 785
Ecl136II GAGCTC 2 cut(s) 287, 734
Eco130I CCWWGG 1 cut(s) 1222
Eco147I AGGCCT 1 cut(s) 1418
Eco24I GRGCYC 3 cut(s) 289, 736, 923
Eco47I GGWCC 4 cut(s) 26, 83, 365, 957
Eco53kI GAGCTC 2 cut(s) 287, 734
Eco57I CTGAAG 1 cut(s) 1187
Eco81I CCTNAGG 1 cut(s) 1468
EcoICRI GAGCTC 2 cut(s) 287, 734
EcoO109I RGGNCCY 1 cut(s) 365
EcoRII CCWGG 3 cut(s) 28, 57, 1333
EcoT14I CCWWGG 1 cut(s) 1222
EcoT38I GRGCYC 3 cut(s) 289, 736, 923
ErhI CCWWGG 1 cut(s) 1222
FaeI CATG 2 cut(s) 581, 643
FalI AAGNNNNNCTT 4 cut(s) 789, 821, 925, 957
FatI CATG 2 cut(s) 577, 639
FbaI TGATCA 1 cut(s) 555
Fnu4HI GCNGC 1 cut(s) 1071
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 3 cut(s) 289, 736, 923
Fsp4HI GCNGC 1 cut(s) 1071
FspBI CTAG 4 cut(s) 320, 476, 701, 1352
FspI TGCGCA 1 cut(s) 1164
GlaI GCGC 2 cut(s) 408, 1164
GluI GCNGC 1 cut(s) 1071
GsuI CTGGAG 2 cut(s) 543, 1410
HaeIII GGCC 2 cut(s) 1019, 1418
HapII CCGG 6 cut(s) 86, 111, 657, 729, 1016, 1326
HhaI GCGC 2 cut(s) 409, 1165
Hin1II CATG 2 cut(s) 581, 643
Hin6I GCGC 2 cut(s) 407, 1163
HinP1I GCGC 2 cut(s) 407, 1163
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 4 cut(s) 316, 653, 686, 1106
HpaII CCGG 6 cut(s) 86, 111, 657, 729, 1016, 1326
HphI GGTGA 2 cut(s) 620, 1154
Hpy166II GTNNAC 2 cut(s) 170, 611
Hpy188I TCNGA 5 cut(s) 506, 782, 850, 1134, 1243
Hpy188III TCNNGA 7 cut(s) 155, 620, 683, 1059, 1205, 1389, 1409
Hpy8I GTNNAC 2 cut(s) 170, 611
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 617, 888
HpyCH4III ACNGT 3 cut(s) 538, 1051, 1261
HpyCH4IV ACGT 4 cut(s) 336, 613, 801, 1189
HpyCH4V TGCA 1 cut(s) 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 4 cut(s) 905, 1410, 1468, 1476
HpySE526I ACGT 4 cut(s) 336, 613, 801, 1189
Hsp92II CATG 2 cut(s) 581, 643
HspAI GCGC 2 cut(s) 407, 1163
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
LmnI GCTCC 7 cut(s) 284, 292, 311, 518, 731, 1018, 1128
Lsp1109I GCAGC 1 cut(s) 1057
LweI GCATC 2 cut(s) 108, 1030
MaeI CTAG 4 cut(s) 320, 476, 701, 1352
MaeII ACGT 4 cut(s) 336, 613, 801, 1189
MaeIII GTNAC 3 cut(s) 181, 1190, 1433
MalI GATC 9 cut(s) 403, 471, 557, 633, 847, 1008, 1098, 1251, 1382
MboI GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
MboII GAAGA 5 cut(s) 464, 817, 1157, 1256, 1397
MflI RGATCY 4 cut(s) 401, 469, 845, 1096
MhlI GDGCHC 4 cut(s) 289, 736, 923, 1273
MlyI GAGTC 1 cut(s) 310
MmeI TCCRAC 2 cut(s) 1150, 1209
MnlI CCTC 7 cut(s) 254, 488, 735, 946, 1055, 1140, 1361
MroXI GAANNNNTTC 2 cut(s) 877, 940
MslI CAYNNNNRTG 3 cut(s) 582, 644, 1038
MspA1I CMGCKG 2 cut(s) 164, 884
MspI CCGG 6 cut(s) 86, 111, 657, 729, 1016, 1326
MspR9I CCNGG 5 cut(s) 30, 59, 111, 729, 1335
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 3 cut(s) 30, 59, 1335
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 2 cut(s) 111, 729
NdeII GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
NlaIII CATG 2 cut(s) 581, 643
NlaIV GGNNCC 3 cut(s) 847, 958, 1330
NmuCI GTSAC 1 cut(s) 181
NsbI TGCGCA 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 1418
PcsI WCGNNNNNNNCGW 2 cut(s) 1002, 1239
PctI GAATGC 1 cut(s) 879
PdmI GAANNNNTTC 2 cut(s) 877, 940
PfeI GAWTC 3 cut(s) 653, 686, 1106
PinAI ACCGGT 1 cut(s) 1325
PkrI GCNGC 1 cut(s) 1072
Ple19I CGATCG 1 cut(s) 1252
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 365
PshBI ATTAAT 2 cut(s) 177, 666
Psp124BI GAGCTC 2 cut(s) 289, 736
Psp1406I AACGTT 1 cut(s) 1189
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 3 cut(s) 28, 57, 1333
PspGI CCWGG 3 cut(s) 28, 57, 1333
PspN4I GGNNCC 3 cut(s) 847, 958, 1330
PspPI GGNCC 4 cut(s) 26, 83, 365, 957
PspPPI RGGWCCY 1 cut(s) 365
PsrI GAACNNNNNNTAC 2 cut(s) 786, 818
PsuI RGATCY 4 cut(s) 401, 469, 845, 1096
PvuI CGATCG 1 cut(s) 1252
RseI CAYNNNNRTG 3 cut(s) 582, 644, 1038
SacI GAGCTC 2 cut(s) 289, 736
SatI GCNGC 1 cut(s) 1071
Sau3AI GATC 9 cut(s) 401, 469, 555, 631, 845, 1006, 1096, 1249, 1380
Sau96I GGNCC 4 cut(s) 26, 83, 365, 957
ScaI AGTACT 1 cut(s) 540
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 5 cut(s) 30, 59, 111, 729, 1335
SduI GDGCHC 4 cut(s) 289, 736, 923, 1273
SfaNI GCATC 2 cut(s) 108, 1030
SfcI CTRYAG 3 cut(s) 228, 243, 369
SinI GGWCC 4 cut(s) 26, 83, 365, 957
SmiMI CAYNNNNRTG 3 cut(s) 582, 644, 1038
SseBI AGGCCT 1 cut(s) 1418
SspI AATATT 3 cut(s) 127, 679, 776
SspMI CTAG 4 cut(s) 320, 476, 701, 1352
SstI GAGCTC 2 cut(s) 289, 736
StuI AGGCCT 1 cut(s) 1418
StyD4I CCNGG 5 cut(s) 28, 57, 109, 727, 1333
StyI CCWWGG 1 cut(s) 1222
TaaI ACNGT 3 cut(s) 538, 1051, 1261
TaiI ACGT 4 cut(s) 339, 616, 804, 1192
TaqI TCGA 4 cut(s) 154, 217, 816, 1440
TaqII GACCGA 1 cut(s) 1019
TatI WGTACW 2 cut(s) 193, 538
TfiI GAWTC 3 cut(s) 653, 686, 1106
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 1070
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 8 cut(s) 386, 662, 684, 731, 735, 807, 1170, 1458
TspGWI ACGGA 2 cut(s) 580, 818
VpaK11BI GGWCC 4 cut(s) 26, 83, 365, 957
VspI ATTAAT 2 cut(s) 177, 666
XapI RAATTY 1 cut(s) 327
XmnI GAANNNNTTC 2 cut(s) 877, 940
XspI CTAG 4 cut(s) 320, 476, 701, 1352
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.