RchiOBHm_Chr1g0336901

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014826
Physical Location & Seq
Forward (+)
28547935 .. 28551178
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56483

Sequence Viewer

Length: 2121 bp
ATGCTCAACTCGCAGGAGGAAGACCATGTTGAACTGAGCATAAACCCAGATTTCCCGCCAGATTACCACGGTTACCCAAGTTACTCAGGCCCCAAAAATGGCCGAATTGATGAAGGGAAATCGGAACCTTCGAGTATTGAGAGAGCACGCTCAGAATCTTCACCACTACTCGCCGCTATATGGGAAAAGCCGTCGACGGTTAAGGGAATCTTCGCAGATGTTTCAAAGTGGGATAAGATCTTTATAACTTCATGCATAATTGCAGTCTTGCTGGATCCATTGTTCTTTTACATTCCATATATCCATGAAGATAACAAGTGCCTTGGAAAAGACAAGACTTTGGGGATTGCAGCTATTCTTCTGCGCTCACTCACAGATATCACTTTCATAGTGAATATTACATATCAGATTCGTGAGTGGATTAAAACTGGTTCCATTAAAATCCAGAAAGCTGGTCCACCCTCCAAGGATGTAGAAGATCAGAGACCAACCATAGTCTCGGAAGATTCGATTGACAAAGGAGAGTGCACTCAATTTGCTGAGGAAGTCGTAGGGATATTTTGCCGAACAAAAAAGAAAACAGCTGGGGCAAAAAATAAGATAGCTCAGAAGTTGCCGTGGTTGACCCTCCCCGTGTTTATTGACATTCTTGCTGTTCTTCCCCTCCCACAAGTGTTTATAGTATATGTTTTCTTCGAAATCAGTGGCTCAGGATATTTGGATTATAGACATATGATGAACCTCTTTCTTCTTGGTCAATATGTGCCTAGGATTTTTCGAATTTATTTATCGTCTGCAGAATTTACAAAAACTAGTGGAATATGGGTTAAAGGTGCATTCAATTTTTTTCTCTACATCCTTGCGAGTCATGTACTTGGAGCATTTTGGTATTTTTTTTCAATTCAACGAGAGACATCATGTTGGCACCGAGCTTGTAAAAAATATATGCATAATGACGAAGGATGTATGAGTAAGTTCTACTGCAAAGGGAATCAAGCTTCTCCGAATACAACTATCACATCATTCCTAGAGGAGCATTGTCCTCTAAGTGACAATATCACATCGGAACAATTTAATTTTGGAATATTTCTTGATGGTCTAAAAAATAATAATACGCAACACATAAATTTTGCACGAAAATTCTTCTTCTCCTTTTGGTGGGGCCTAAGAAACCTAAGTAATTTTGGCACAAATCTGACAGAAACAAGTACCTATGTGTGGGAAAACTTATTCGCCATTCTTATCTCCATCATTGGCTTGCTACTGTTTCTTTATCTCATTGGAAATGTGCAGACACTAATGCAGTTTGAAACTACAAAATGGGAGGAGATAAGACGGAAGATTGAAATGAAAAGGCTCGATCTAGAAGTGTGGATGCAAAGAAATAAAATCCCGAATGAGCTGAAGAAAAAGATCATTAAGAGCATAAAAAAGAAGTTGGAAGAAGACAAAGATGCCAATCTTGAAAATCTATTCAGTATTCTTCCATGGGATACCAGAAAAGATCTTAAGCGTTTTCTCTGCGTGAATGCGCTAAGAAATGAGCCGATGCTCAAAGATGCTAATCCAAAAGTGGTGAAAATGATCTGCGACTATCTGAAGCCGGTGATGTATGTGGAGAAGAGCAGTATCTTTCAAGCCGGAGAACCACTCGACATGATGCTTTTCATAACGCAAGGCACTGTTTGGACCTATTCGAGTAGTAGTACAAGTGATGATACTTCTCTAACAGGAAAACAAAATCTGGGTAGTGCTTCTCCAATAATGATCACCAAGTCACTCAAGGAAGGCAATTCTTATGGAGTTGAACAACTTCTACGTTGGGTAGCAGTACCCGACGAGCATTTCTCCAACCTTCCTCGCCTGAACGAGAATGTGAGATCTCATTCAAAAGTGGAAGGCTTTACACTCATGGCCAGCGACTTGAGAAAGGTAGCCATCAAATGCCAAGGCATTTGGAACTTCATCAATGCCCCCAATCCTCAAGAAAGAGAGGAGGTGGCACGTACTACGATAGCCGCAGCAGTCCGTCGTTTCCGAAATCAGCTACAAGCACCAAAGAAGTCCAGATCAAAGCCGTCGGCAGTTGGGGCTCAGAACTCGCCAATAAACATCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

706

Amino Acids

80.82

Weight (kDa)

8.9

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 213 - 440 7.1e-08 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 924
AccI GTMKAC 1 cut(s) 194
AciI CCGC 3 cut(s) 56, 174, 2019
AclWI GGATC 2 cut(s) 269, 282
AcoI YGGCCR 2 cut(s) 100, 1914
AcsI RAATTY 4 cut(s) 780, 800, 1126, 1139
AcuI CTGAAG 2 cut(s) 1424, 1619
AfaI GTAC 5 cut(s) 873, 1210, 1708, 1833, 2008
AfiI CCNNNNNNNGG 4 cut(s) 98, 180, 1158, 1218
AflII CTTAAG 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 812
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 8 cut(s) 353, 452, 584, 605, 932, 998, 1402, 2047
AluI AGCT 8 cut(s) 353, 452, 584, 605, 932, 998, 1402, 2047
Alw21I GWGCWC 2 cut(s) 148, 530
Alw26I GTCTC 3 cut(s) 478, 502, 905
Alw44I GTGCAC 1 cut(s) 526
AlwI GGATC 2 cut(s) 269, 282
AoxI GGCC 4 cut(s) 88, 100, 1162, 1914
ApaLI GTGCAC 1 cut(s) 526
ApeKI GCWGC 2 cut(s) 350, 2021
ApoI RAATTY 4 cut(s) 780, 800, 1126, 1139
Asp700I GAANNNNTTC 1 cut(s) 1812
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 2 cut(s) 366, 1534
AspS9I GGNCC 4 cut(s) 89, 455, 1162, 1689
AsuHPI GGTGA 4 cut(s) 153, 1588, 1618, 1762
AsuII TTCGAA 2 cut(s) 696, 778
AvaII GGWCC 2 cut(s) 455, 1689
AvrII CCTAGG 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 530
BalI TGGCCA 1 cut(s) 1916
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 924
BanII GRGCYC 1 cut(s) 2095
BbsI GAAGAC 2 cut(s) 27, 1452
Bbv12I GWGCWC 2 cut(s) 148, 530
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 362, 2033
BccI CCATC 3 cut(s) 1088, 1256, 1946
BceAI ACGGC 3 cut(s) 175, 601, 2062
BciVI GTATCC 1 cut(s) 1486
BclI TGATCA 1 cut(s) 1767
BcoDI GTCTC 3 cut(s) 478, 502, 905
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 4 cut(s) 768, 813, 1028, 1364
BfmI CTRYAG 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 1508
BfuI GTATCC 1 cut(s) 1486
BglII AGATCT 3 cut(s) 237, 1504, 1880
BisI GCNGC 4 cut(s) 174, 351, 2019, 2022
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 4 cut(s) 175, 352, 2020, 2023
Bme18I GGWCC 2 cut(s) 455, 1689
BmgT120I GGNCC 4 cut(s) 89, 455, 1162, 1689
BmiI GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
BmsI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
BpiI GAAGAC 2 cut(s) 27, 1452
BplI GAGNNNNNCTC 4 cut(s) 1635, 1667, 1832, 1864
Bpu10I CCTNAGC 2 cut(s) 540, 709
Bpu14I TTCGAA 2 cut(s) 696, 778
BpuEI CTTGAG 3 cut(s) 1766, 1945, 1968
BsaAI YACGTR 1 cut(s) 2006
BsaBI GATNNNNATC 2 cut(s) 1458, 1563
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 7 cut(s) 67, 322, 465, 617, 767, 1487, 1948
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 180, 1158, 1218
Bse118I RCCGGY 1 cut(s) 1603
Bse1I ACTGG 1 cut(s) 433
Bse8I GATNNNNATC 2 cut(s) 1458, 1563
BseDI CCNNGG 7 cut(s) 67, 322, 465, 617, 767, 1487, 1948
BseGI GGATG 4 cut(s) 475, 855, 968, 1380
BseJI GATNNNNATC 2 cut(s) 1458, 1563
BseLI CCNNNNNNNGG 4 cut(s) 98, 180, 1158, 1218
BseMII CTCAG 7 cut(s) 26, 99, 165, 531, 620, 723, 2108
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 1046, 1340, 2009
BseSI GKGCMC 1 cut(s) 530
BseXI GCAGC 2 cut(s) 362, 2033
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 1310
BshFI GGCC 4 cut(s) 90, 102, 1164, 1916
BshNI GGYRCC 1 cut(s) 924
BsiHKAI GWGCWC 2 cut(s) 148, 530
BsiSI CCGG 2 cut(s) 1604, 1641
BslI CCNNNNNNNGG 4 cut(s) 98, 180, 1158, 1218
BsmAI GTCTC 3 cut(s) 478, 502, 905
BsmI GAATGC 2 cut(s) 836, 1534
BsnI GGCC 4 cut(s) 90, 102, 1164, 1916
Bso31I GGTCTC 1 cut(s) 478
Bsp119I TTCGAA 2 cut(s) 696, 778
Bsp1286I GDGCHC 3 cut(s) 148, 530, 2095
Bsp19I CCATGG 1 cut(s) 1487
BspACI CCGC 3 cut(s) 56, 174, 2019
BspANI GGCC 4 cut(s) 90, 102, 1164, 1916
BspCNI CTCAG 7 cut(s) 27, 98, 164, 532, 619, 722, 2107
BspLI GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
BspMAI CTGCAG 1 cut(s) 799
BspPI GGATC 2 cut(s) 269, 282
BspQI GCTCTTC 1 cut(s) 1616
BspT104I TTCGAA 2 cut(s) 696, 778
BspT107I GGYRCC 1 cut(s) 924
BspTI CTTAAG 1 cut(s) 1508
BspTNI GGTCTC 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 1603
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 1603
BssECI CCNNGG 7 cut(s) 67, 322, 465, 617, 767, 1487, 1948
BssT1I CCWWGG 5 cut(s) 322, 465, 767, 1487, 1948
Bst4CI ACNGT 4 cut(s) 71, 199, 1266, 1684
Bst6I CTCTTC 1 cut(s) 1616
BstAFI CTTAAG 1 cut(s) 1508
BstBAI YACGTR 1 cut(s) 2006
BstBI TTCGAA 2 cut(s) 696, 778
BstC8I GCNNGC 3 cut(s) 148, 1259, 1918
BstDSI CCRYGG 3 cut(s) 67, 617, 1487
BstEII GGTNACC 1 cut(s) 71
BstF5I GGATG 4 cut(s) 475, 855, 968, 1380
BstHHI GCGC 2 cut(s) 366, 1534
BstMAI GTCTC 3 cut(s) 478, 502, 905
BstMWI GCNNNNNNNGC 2 cut(s) 10, 2090
BstPI GGTNACC 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 1 cut(s) 530
BstV1I GCAGC 2 cut(s) 362, 2033
BstV2I GAAGAC 2 cut(s) 27, 1452
BstX2I RGATCY 4 cut(s) 237, 274, 1504, 1880
BstXI CCANNNNNNTGG 1 cut(s) 452
BstYI RGATCY 4 cut(s) 237, 274, 1504, 1880
BsuI GTATCC 1 cut(s) 1486
BsuRI GGCC 4 cut(s) 90, 102, 1164, 1916
BtgI CCRYGG 3 cut(s) 67, 617, 1487
BtsCI GGATG 4 cut(s) 475, 855, 968, 1380
BtsIMutI CAGTG 2 cut(s) 709, 1680
Cac8I GCNNGC 3 cut(s) 148, 1259, 1918
CfoI GCGC 2 cut(s) 366, 1534
Cfr10I RCCGGY 1 cut(s) 1603
Cfr13I GGNCC 4 cut(s) 89, 455, 1162, 1689
Csp6I GTAC 5 cut(s) 872, 1209, 1707, 1832, 2007
CviAII CATG 8 cut(s) 26, 252, 305, 869, 918, 1488, 1657, 1912
CviQI GTAC 5 cut(s) 872, 1209, 1707, 1832, 2007
EaeI YGGCCR 2 cut(s) 100, 1914
Eam1104I CTCTTC 1 cut(s) 1616
EarI CTCTTC 1 cut(s) 1616
Eco130I CCWWGG 5 cut(s) 322, 465, 767, 1487, 1948
Eco24I GRGCYC 1 cut(s) 2095
Eco31I GGTCTC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 455, 1689
Eco57I CTGAAG 2 cut(s) 1424, 1619
Eco91I GGTNACC 1 cut(s) 71
EcoO109I RGGNCCY 2 cut(s) 89, 1162
EcoO65I GGTNACC 1 cut(s) 71
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 5 cut(s) 322, 465, 767, 1487, 1948
EcoT22I ATGCAT 2 cut(s) 257, 951
EcoT38I GRGCYC 1 cut(s) 2095
ErhI CCWWGG 5 cut(s) 322, 465, 767, 1487, 1948
FaeI CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1915
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 8 cut(s) 25, 251, 304, 868, 917, 1487, 1656, 1911
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 1 cut(s) 732
FbaI TGATCA 1 cut(s) 1767
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 4 cut(s) 174, 351, 2019, 2022
FokI GGATG 4 cut(s) 482, 842, 975, 1387
FriOI GRGCYC 1 cut(s) 2095
Fsp4HI GCNGC 4 cut(s) 174, 351, 2019, 2022
FspBI CTAG 4 cut(s) 768, 813, 1028, 1364
GlaI GCGC 2 cut(s) 365, 1533
GluI GCNGC 4 cut(s) 174, 351, 2019, 2022
GsaI CCCAGC 1 cut(s) 588
HaeIII GGCC 4 cut(s) 90, 102, 1164, 1916
HapII CCGG 2 cut(s) 1604, 1641
HhaI GCGC 2 cut(s) 366, 1534
Hin1II CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1915
Hin6I GCGC 2 cut(s) 364, 1532
HinP1I GCGC 2 cut(s) 364, 1532
HincII GTYRAC 2 cut(s) 195, 624
HindII GTYRAC 2 cut(s) 195, 624
HindIII AAGCTT 1 cut(s) 996
HinfI GANTC 6 cut(s) 155, 207, 409, 506, 865, 991
HpaII CCGG 2 cut(s) 1604, 1641
HphI GGTGA 4 cut(s) 153, 1588, 1618, 1762
Hpy166II GTNNAC 4 cut(s) 195, 458, 528, 624
Hpy188III TCNNGA 9 cut(s) 413, 445, 711, 1091, 1364, 1393, 1463, 1985, 2067
Hpy8I GTNNAC 4 cut(s) 195, 458, 528, 624
Hpy99I CGWCG 5 cut(s) 196, 199, 1841, 2034, 2083
HpyAV CCTTC 6 cut(s) 107, 138, 953, 1781, 1865, 1892
HpyCH4III ACNGT 4 cut(s) 71, 199, 1266, 1684
HpyCH4IV ACGT 2 cut(s) 1819, 2005
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 2090
HpySE526I ACGT 2 cut(s) 1819, 2005
Hsp92II CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1915
HspAI GCGC 2 cut(s) 364, 1532
Ksp22I TGATCA 1 cut(s) 1767
LguI GCTCTTC 1 cut(s) 1616
LmnI GCTCC 2 cut(s) 878, 1033
Lsp1109I GCAGC 2 cut(s) 362, 2033
LweI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
MaeI CTAG 4 cut(s) 768, 813, 1028, 1364
MaeII ACGT 2 cut(s) 1819, 2005
MaeIII GTNAC 4 cut(s) 71, 80, 1049, 1776
MflI RGATCY 4 cut(s) 237, 274, 1504, 1880
MhlI GDGCHC 3 cut(s) 148, 530, 2095
MlsI TGGCCA 1 cut(s) 1916
MluNI TGGCCA 1 cut(s) 1916
MlyI GAGTC 1 cut(s) 874
MmeI TCCRAC 2 cut(s) 1419, 1875
Mox20I TGGCCA 1 cut(s) 1916
Mph1103I ATGCAT 2 cut(s) 257, 951
MroXI GAANNNNTTC 1 cut(s) 1812
MscI TGGCCA 1 cut(s) 1916
MseI TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
Msp20I TGGCCA 1 cut(s) 1916
MspA1I CMGCKG 1 cut(s) 584
MspCI CTTAAG 1 cut(s) 1508
MspI CCGG 2 cut(s) 1604, 1641
Mva1269I GAATGC 2 cut(s) 836, 1534
MwoI GCNNNNNNNGC 2 cut(s) 10, 2090
NcoI CCATGG 1 cut(s) 1487
NdeI CATATG 1 cut(s) 732
NlaIII CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1915
NlaIV GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
NmuCI GTSAC 2 cut(s) 1049, 1776
NsiI ATGCAT 2 cut(s) 257, 951
NspV TTCGAA 2 cut(s) 696, 778
PciSI GCTCTTC 1 cut(s) 1616
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 506
PctI GAATGC 2 cut(s) 836, 1534
PdmI GAANNNNTTC 1 cut(s) 1812
PfeI GAWTC 5 cut(s) 155, 207, 409, 506, 991
PkrI GCNGC 4 cut(s) 175, 352, 2020, 2023
PleI GAGTC 1 cut(s) 873
PpsI GAGTC 1 cut(s) 873
Ppu21I YACGTR 1 cut(s) 2006
PsiI TTATAA 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 71
PspFI CCCAGC 1 cut(s) 584
PspN4I GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
PspPI GGNCC 4 cut(s) 89, 455, 1162, 1689
PsrI GAACNNNNNNTAC 2 cut(s) 1800, 1832
PstI CTGCAG 1 cut(s) 799
PsuI RGATCY 4 cut(s) 237, 274, 1504, 1880
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 5 cut(s) 873, 1210, 1708, 1833, 2008
RsaNI GTAC 5 cut(s) 872, 1209, 1707, 1832, 2007
SalI GTCGAC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 1616
SaqAI TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
SatI GCNGC 4 cut(s) 174, 351, 2019, 2022
Sau96I GGNCC 4 cut(s) 89, 455, 1162, 1689
SchI GAGTC 1 cut(s) 874
SduI GDGCHC 3 cut(s) 148, 530, 2095
SfaNI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
SfcI CTRYAG 1 cut(s) 795
SfuI TTCGAA 2 cut(s) 696, 778
SgrDI CGTCGACG 1 cut(s) 193
SinI GGWCC 2 cut(s) 455, 1689
SmlI CTYRAG 4 cut(s) 1508, 1781, 1924, 1983
SmoI CTYRAG 4 cut(s) 1508, 1781, 1924, 1983
SpeI ACTAGT 1 cut(s) 812
SsiI CCGC 3 cut(s) 56, 174, 2019
SspI AATATT 2 cut(s) 397, 1086
SspMI CTAG 4 cut(s) 768, 813, 1028, 1364
StyI CCWWGG 5 cut(s) 322, 465, 767, 1487, 1948
TaaI ACNGT 4 cut(s) 71, 199, 1266, 1684
TaiI ACGT 2 cut(s) 1822, 2008
TaqI TCGA 8 cut(s) 131, 194, 509, 696, 778, 1359, 1653, 1697
TatI WGTACW 2 cut(s) 871, 1706
TauI GCSGC 2 cut(s) 176, 2021
TfiI GAWTC 5 cut(s) 155, 207, 409, 506, 991
Tru1I TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
Tru9I TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
TscAI CASTG 2 cut(s) 709, 1687
TseFI GTSAC 2 cut(s) 1049, 1776
TseI GCWGC 2 cut(s) 350, 2021
Tsp45I GTSAC 2 cut(s) 1049, 1776
TspDTI ATGAA 8 cut(s) 126, 240, 321, 376, 752, 1364, 1657, 1954
TspGWI ACGGA 2 cut(s) 1351, 2018
TspRI CASTG 2 cut(s) 709, 1687
Vha464I CTTAAG 1 cut(s) 1508
VneI GTGCAC 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 455, 1689
XapI RAATTY 4 cut(s) 780, 800, 1126, 1139
XbaI TCTAGA 1 cut(s) 1363
XmaJI CCTAGG 1 cut(s) 767
XmiI GTMKAC 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 1812
XspI CTAG 4 cut(s) 768, 813, 1028, 1364
Zsp2I ATGCAT 2 cut(s) 257, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.