Rw1G012090

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014826
Physical Location & Seq
Forward (+)
27038562 .. 27041842
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012090.1

Sequence Viewer

Length: 2073 bp
ATGCTCAACTCGCAGGAGGAAGACCATGTTGAACTGAGCATAAACCCAGATTTCCCGCCAGATTACCACAGTTACCCAAGTTACTCAGGCCCCAAAAATGGCCGAATTGATGAAGGGAAATCGGAACCTTCGAGTATTGAGAGAGCACGCTCAGAATCTTCACCACTACTCGCCGCTATATGGGAAAAGCCGTCGACGGTTAAGGGAATCTTCGCAGATGTTTCAAAGTGGGATAAGATCTTTATAACTTCATGCATAATTGCAGTCTTGTTGGATCCATTGTTCTTTTACATTCCATATATCCATGAAGATAACAAGTGCCTTGGAAAAGACAAGACTTTGGGGATTGCAGCTATTCTTCTGCGCTCACTCACAGATATCACTTTCATAGTGAATATTACATATCAGATTCGTGAGTGGATTAAAACTGGTTCCATTAAAATCCAGAAAGCTGGTCTACCCTCCAAGGATGTAGAAGATCAAAGACCAACCATAGTCTCGGGAGACTCGATTGACAAAGGAGAGTGCACTCAATTTGCTGAGGAAGTCGTAGGGATATTTAGCCGAACAAAAAAGAAAATAGCTGGGGCAAAAAATAAGATAGCTCAGAAGTTGCAGTGGTTGACCCTCCCCGTGTTTATTGACATTCTTGCTGTTCTTCCCCTCCCACAAGTGTTTATAGTATATGTTTTCTTCGAAATCAGTGGCTCAGGATATTTGGATTATAGACATATGATGAACCTCTTTCTTCTTGGTCAATATGTGCCTAGGATTTTTCGAATTTATTTATCGTCTGCAGAATTTACAAAAACTAGTGGAATATGGGTTAAAGGTGCATTCAATTTTTTTCTCTACATCCTTGCGAGTCATGTACTCGGAGCATTTTGGTATTTTTTTTCAATTCAACGAGAGACATCATGTTGGCACCGAGCTTGTCAAAAATATATGCATAATGACGAAGGATGTATGAGTAAGTTTTACTGCAAAGGGAATCAAGCTTCTCCGAATACAACTATCACATCATTCCTAGAGGAGCATTGTCCTCTAAGTGACAATATCACATCGGAACAATTTAATTTTGGAATGTTTCTTGATGGCCTAAAAAATAATAATACGCAACACATAAATTTTGCACGAAAGTTCTTCTTCTCCTTTTGGTGGGGCCTAAGAAACCTAAGTAATTTTGGCACAAATCTGACAGAAACAAGTACCTATGTGTGGGAAAACTTATTCGCCATTCTTATCTCTATCATTGGCTTGCTACTGTTCCTATATCTCATTGGAAATGTGCAGACACTAATGCAGTTTGAAACTTCAAAATGGGAGGAGATAAGACGGAAGATTGAAATGAAAAGGCTCGATCTAGAAGTGTGGATGCAAAGAAATAAAATCCCGAATGAGCTGAAGAAAAAGATCATTAAGAGCATAAAAAAGAAGTTGGAAGAAGACAAAGATGCCAATCTTGAAAATCTATTCAGTATTCTTCCATGGGATACCAGAAAAGATCTTAAGCGTTTTCTCTGCGTGAATGCGCTAAGAAATGAGCCGATGCTCAAAGATGCTAATCCAAAAGTGGTGAAAATGATCTGCGACTATCTGAAGCCGGTGATGTATGTGGAGAAGAGCAGTATCTTTCAAGCCGGAGAACCACTCGACATGATGCTTTTCATAACTCAAGGCACTGTTTGGACCTATTCGAGTAGTAGTACAAGTGATGATACTTCTCTAACAGGAAAACAAAAAATGGGTAGTGCTTCTCCAATAATGATCACCAAGGCACTCAAGGAAGGCAATTCTTATGGAGTTGAACAACTTCTACGTTGGAATGTGAGATCTCATTCAAAAGTGGAAGGCTTTACACTCATGGCCAGCGACTTGAGAAAGGTAGCCATCAAATGCCAAGGCATTTGGAACTTCATCAATGCCCCCAATCCTCAAGAAAGAGAGGAGGTGGCACGTACTACGATAGCCGCAGCAGTCCGTCGTTTCCGAAATCAGCTACAAGCACCAAAGAAGTCCGGATCAAAGCCGCCGGCAGTTGGGGCTCAGAACTCGCCAATAAACATCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

690

Amino Acids

78.93

Weight (kDa)

9.02

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 213 - 441 2.4e-08 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 924
AccI GTMKAC 2 cut(s) 194, 457
AccIII TCCGGA 1 cut(s) 2018
AciI CCGC 4 cut(s) 56, 174, 1971, 2030
AclWI GGATC 3 cut(s) 269, 282, 2029
AcoI YGGCCR 2 cut(s) 100, 1866
AcsI RAATTY 3 cut(s) 780, 800, 1126
AcuI CTGAAG 2 cut(s) 1424, 1619
AfaI GTAC 4 cut(s) 873, 1210, 1708, 1960
AfiI CCNNNNNNNGG 5 cut(s) 98, 180, 1158, 1218, 2039
AflII CTTAAG 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 812
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 8 cut(s) 353, 452, 584, 605, 932, 998, 1402, 1999
AluI AGCT 8 cut(s) 353, 452, 584, 605, 932, 998, 1402, 1999
Alw21I GWGCWC 2 cut(s) 148, 530
Alw26I GTCTC 3 cut(s) 498, 502, 905
Alw44I GTGCAC 1 cut(s) 526
AlwI GGATC 3 cut(s) 269, 282, 2029
Ama87I CYCGRG 1 cut(s) 499
Aor13HI TCCGGA 1 cut(s) 2018
AoxI GGCC 5 cut(s) 88, 100, 1096, 1162, 1866
ApaLI GTGCAC 1 cut(s) 526
ApeKI GCWGC 2 cut(s) 350, 1973
ApoI RAATTY 3 cut(s) 780, 800, 1126
Asp700I GAANNNNTTC 1 cut(s) 1812
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 2 cut(s) 366, 1534
AspS9I GGNCC 3 cut(s) 89, 1162, 1689
AsuHPI GGTGA 4 cut(s) 153, 1588, 1618, 1762
AsuII TTCGAA 2 cut(s) 696, 778
AvaI CYCGRG 1 cut(s) 499
AvaII GGWCC 1 cut(s) 1689
AvrII CCTAGG 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 530
BalI TGGCCA 1 cut(s) 1868
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 924
BanII GRGCYC 1 cut(s) 2047
BbsI GAAGAC 2 cut(s) 27, 1452
Bbv12I GWGCWC 2 cut(s) 148, 530
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 362, 1985
BccI CCATC 2 cut(s) 1088, 1898
BceAI ACGGC 1 cut(s) 175
BciVI GTATCC 1 cut(s) 1486
BclI TGATCA 1 cut(s) 1767
BcoDI GTCTC 3 cut(s) 498, 502, 905
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 4 cut(s) 768, 813, 1028, 1364
BfmI CTRYAG 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 1508
BfuI GTATCC 1 cut(s) 1486
BglII AGATCT 3 cut(s) 237, 1504, 1832
BisI GCNGC 5 cut(s) 174, 351, 1971, 1974, 2030
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 5 cut(s) 175, 352, 1972, 1975, 2031
Bme18I GGWCC 1 cut(s) 1689
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 3 cut(s) 89, 1162, 1689
BmiI GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
BmsI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
BpiI GAAGAC 2 cut(s) 27, 1452
BplI GAGNNNNNCTC 2 cut(s) 1635, 1667
Bpu10I CCTNAGC 2 cut(s) 540, 709
Bpu14I TTCGAA 2 cut(s) 696, 778
BpuEI CTTGAG 4 cut(s) 1659, 1766, 1897, 1920
BsaAI YACGTR 1 cut(s) 1958
BsaBI GATNNNNATC 2 cut(s) 1458, 1563
BsaJI CCNNGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
BsaWI WCCGGW 1 cut(s) 2018
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 180, 1158, 1218, 2039
Bse118I RCCGGY 2 cut(s) 1603, 2032
Bse1I ACTGG 1 cut(s) 433
Bse8I GATNNNNATC 2 cut(s) 1458, 1563
BseAI TCCGGA 1 cut(s) 2018
BseDI CCNNGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
BseGI GGATG 4 cut(s) 475, 855, 968, 1380
BseJI GATNNNNATC 2 cut(s) 1458, 1563
BseLI CCNNNNNNNGG 5 cut(s) 98, 180, 1158, 1218, 2039
BseMII CTCAG 7 cut(s) 26, 99, 165, 531, 620, 723, 2060
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 1046, 1340, 1961
BseSI GKGCMC 1 cut(s) 530
BseXI GCAGC 2 cut(s) 362, 1985
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 1310
BshFI GGCC 5 cut(s) 90, 102, 1098, 1164, 1868
BshNI GGYRCC 1 cut(s) 924
BsiHKAI GWGCWC 2 cut(s) 148, 530
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 4 cut(s) 1604, 1641, 2019, 2033
BslI CCNNNNNNNGG 5 cut(s) 98, 180, 1158, 1218, 2039
BsmAI GTCTC 3 cut(s) 498, 502, 905
BsmI GAATGC 2 cut(s) 836, 1534
BsnI GGCC 5 cut(s) 90, 102, 1098, 1164, 1868
BsoBI CYCGRG 1 cut(s) 499
Bsp119I TTCGAA 2 cut(s) 696, 778
Bsp1286I GDGCHC 3 cut(s) 148, 530, 2047
Bsp13I TCCGGA 1 cut(s) 2018
Bsp19I CCATGG 1 cut(s) 1487
BspACI CCGC 4 cut(s) 56, 174, 1971, 2030
BspANI GGCC 5 cut(s) 90, 102, 1098, 1164, 1868
BspCNI CTCAG 7 cut(s) 27, 98, 164, 532, 619, 722, 2059
BspEI TCCGGA 1 cut(s) 2018
BspLI GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
BspMAI CTGCAG 1 cut(s) 799
BspPI GGATC 3 cut(s) 269, 282, 2029
BspQI GCTCTTC 1 cut(s) 1616
BspT104I TTCGAA 2 cut(s) 696, 778
BspT107I GGYRCC 1 cut(s) 924
BspTI CTTAAG 1 cut(s) 1508
BsrFI RCCGGY 2 cut(s) 1603, 2032
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 2 cut(s) 1603, 2032
BssECI CCNNGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
BssT1I CCWWGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
Bst4CI ACNGT 4 cut(s) 71, 199, 1266, 1684
Bst6I CTCTTC 1 cut(s) 1616
BstAFI CTTAAG 1 cut(s) 1508
BstBAI YACGTR 1 cut(s) 1958
BstBI TTCGAA 2 cut(s) 696, 778
BstC8I GCNNGC 4 cut(s) 148, 1259, 1870, 2034
BstDSI CCRYGG 1 cut(s) 1487
BstF5I GGATG 4 cut(s) 475, 855, 968, 1380
BstHHI GCGC 2 cut(s) 366, 1534
BstMAI GTCTC 3 cut(s) 498, 502, 905
BstMWI GCNNNNNNNGC 2 cut(s) 10, 2042
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 1 cut(s) 530
BstV1I GCAGC 2 cut(s) 362, 1985
BstV2I GAAGAC 2 cut(s) 27, 1452
BstX2I RGATCY 4 cut(s) 237, 274, 1504, 1832
BstXI CCANNNNNNTGG 1 cut(s) 452
BstYI RGATCY 4 cut(s) 237, 274, 1504, 1832
BsuI GTATCC 1 cut(s) 1486
BsuRI GGCC 5 cut(s) 90, 102, 1098, 1164, 1868
BtgI CCRYGG 1 cut(s) 1487
BtsCI GGATG 4 cut(s) 475, 855, 968, 1380
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 3 cut(s) 623, 709, 1680
Cac8I GCNNGC 4 cut(s) 148, 1259, 1870, 2034
CfoI GCGC 2 cut(s) 366, 1534
Cfr10I RCCGGY 2 cut(s) 1603, 2032
Cfr13I GGNCC 3 cut(s) 89, 1162, 1689
Csp6I GTAC 4 cut(s) 872, 1209, 1707, 1959
CviAII CATG 8 cut(s) 26, 252, 305, 869, 918, 1488, 1657, 1864
CviQI GTAC 4 cut(s) 872, 1209, 1707, 1959
EaeI YGGCCR 2 cut(s) 100, 1866
Eam1104I CTCTTC 1 cut(s) 1616
EarI CTCTTC 1 cut(s) 1616
Eco130I CCWWGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
Eco24I GRGCYC 1 cut(s) 2047
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 1689
Eco57I CTGAAG 2 cut(s) 1424, 1619
Eco88I CYCGRG 1 cut(s) 499
EcoO109I RGGNCCY 2 cut(s) 89, 1162
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
EcoT22I ATGCAT 2 cut(s) 257, 951
EcoT38I GRGCYC 1 cut(s) 2047
ErhI CCWWGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
FaeI CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1867
FalI AAGNNNNNCTT 4 cut(s) 194, 226, 1130, 1162
FatI CATG 8 cut(s) 25, 251, 304, 868, 917, 1487, 1656, 1863
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 1 cut(s) 732
FbaI TGATCA 1 cut(s) 1767
FblI GTMKAC 2 cut(s) 194, 457
Fnu4HI GCNGC 5 cut(s) 174, 351, 1971, 1974, 2030
FokI GGATG 4 cut(s) 482, 842, 975, 1387
FriOI GRGCYC 1 cut(s) 2047
Fsp4HI GCNGC 5 cut(s) 174, 351, 1971, 1974, 2030
FspBI CTAG 4 cut(s) 768, 813, 1028, 1364
GlaI GCGC 2 cut(s) 365, 1533
GluI GCNGC 5 cut(s) 174, 351, 1971, 1974, 2030
GsaI CCCAGC 1 cut(s) 588
HaeIII GGCC 5 cut(s) 90, 102, 1098, 1164, 1868
HapII CCGG 4 cut(s) 1604, 1641, 2019, 2033
HhaI GCGC 2 cut(s) 366, 1534
Hin1II CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1867
Hin6I GCGC 2 cut(s) 364, 1532
HinP1I GCGC 2 cut(s) 364, 1532
HincII GTYRAC 2 cut(s) 195, 624
HindII GTYRAC 2 cut(s) 195, 624
HindIII AAGCTT 1 cut(s) 996
HinfI GANTC 6 cut(s) 155, 207, 409, 506, 865, 991
HpaII CCGG 4 cut(s) 1604, 1641, 2019, 2033
HphI GGTGA 4 cut(s) 153, 1588, 1618, 1762
Hpy166II GTNNAC 4 cut(s) 195, 458, 528, 624
Hpy8I GTNNAC 4 cut(s) 195, 458, 528, 624
Hpy99I CGWCG 3 cut(s) 196, 199, 1986
HpyAV CCTTC 5 cut(s) 107, 138, 953, 1781, 1844
HpyCH4III ACNGT 4 cut(s) 71, 199, 1266, 1684
HpyCH4IV ACGT 2 cut(s) 1819, 1957
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 2042
HpySE526I ACGT 2 cut(s) 1819, 1957
Hsp92II CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1867
HspAI GCGC 2 cut(s) 364, 1532
Kpn2I TCCGGA 1 cut(s) 2018
KroI GCCGGC 1 cut(s) 2032
KroNI GCCGGC 1 cut(s) 2034
Ksp22I TGATCA 1 cut(s) 1767
LguI GCTCTTC 1 cut(s) 1616
LmnI GCTCC 2 cut(s) 878, 1033
Lsp1109I GCAGC 2 cut(s) 362, 1985
LweI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
MaeI CTAG 4 cut(s) 768, 813, 1028, 1364
MaeII ACGT 2 cut(s) 1819, 1957
MaeIII GTNAC 3 cut(s) 71, 80, 1049
MflI RGATCY 4 cut(s) 237, 274, 1504, 1832
MhlI GDGCHC 3 cut(s) 148, 530, 2047
MlsI TGGCCA 1 cut(s) 1868
MluNI TGGCCA 1 cut(s) 1868
MlyI GAGTC 2 cut(s) 500, 874
MmeI TCCRAC 3 cut(s) 252, 1419, 1802
Mox20I TGGCCA 1 cut(s) 1868
Mph1103I ATGCAT 2 cut(s) 257, 951
MroI TCCGGA 1 cut(s) 2018
MroNI GCCGGC 1 cut(s) 2032
MroXI GAANNNNTTC 1 cut(s) 1812
MscI TGGCCA 1 cut(s) 1868
MseI TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
Msp20I TGGCCA 1 cut(s) 1868
MspCI CTTAAG 1 cut(s) 1508
MspI CCGG 4 cut(s) 1604, 1641, 2019, 2033
Mva1269I GAATGC 2 cut(s) 836, 1534
MwoI GCNNNNNNNGC 2 cut(s) 10, 2042
NaeI GCCGGC 1 cut(s) 2034
NcoI CCATGG 1 cut(s) 1487
NdeI CATATG 1 cut(s) 732
NgoMIV GCCGGC 1 cut(s) 2032
NlaIII CATG 8 cut(s) 29, 255, 308, 872, 921, 1491, 1660, 1867
NlaIV GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
NmuCI GTSAC 1 cut(s) 1049
NsiI ATGCAT 2 cut(s) 257, 951
NspV TTCGAA 2 cut(s) 696, 778
PciSI GCTCTTC 1 cut(s) 1616
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 506
PctI GAATGC 2 cut(s) 836, 1534
PdiI GCCGGC 1 cut(s) 2034
PdmI GAANNNNTTC 1 cut(s) 1812
PfeI GAWTC 4 cut(s) 155, 207, 409, 991
PkrI GCNGC 5 cut(s) 175, 352, 1972, 1975, 2031
PleI GAGTC 2 cut(s) 500, 873
PpsI GAGTC 2 cut(s) 500, 873
Ppu21I YACGTR 1 cut(s) 1958
PsiI TTATAA 1 cut(s) 245
PspFI CCCAGC 1 cut(s) 584
PspN4I GGNNCC 6 cut(s) 91, 126, 276, 433, 926, 1163
PspPI GGNCC 3 cut(s) 89, 1162, 1689
PsrI GAACNNNNNNTAC 2 cut(s) 1800, 1832
PstI CTGCAG 1 cut(s) 799
PsuI RGATCY 4 cut(s) 237, 274, 1504, 1832
RsaI GTAC 4 cut(s) 873, 1210, 1708, 1960
RsaNI GTAC 4 cut(s) 872, 1209, 1707, 1959
SalI GTCGAC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 1616
SaqAI TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
SatI GCNGC 5 cut(s) 174, 351, 1971, 1974, 2030
Sau96I GGNCC 3 cut(s) 89, 1162, 1689
SchI GAGTC 2 cut(s) 500, 874
SduI GDGCHC 3 cut(s) 148, 530, 2047
SfaNI GCATC 5 cut(s) 1365, 1444, 1539, 1549, 1650
SfcI CTRYAG 1 cut(s) 795
SfuI TTCGAA 2 cut(s) 696, 778
SgrDI CGTCGACG 1 cut(s) 193
SinI GGWCC 1 cut(s) 1689
SmlI CTYRAG 5 cut(s) 1508, 1674, 1781, 1876, 1935
SmoI CTYRAG 5 cut(s) 1508, 1674, 1781, 1876, 1935
SpeI ACTAGT 1 cut(s) 812
SsiI CCGC 4 cut(s) 56, 174, 1971, 2030
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 768, 813, 1028, 1364
StyI CCWWGG 6 cut(s) 322, 465, 767, 1487, 1773, 1900
TaaI ACNGT 4 cut(s) 71, 199, 1266, 1684
TaiI ACGT 2 cut(s) 1822, 1960
TaqI TCGA 8 cut(s) 131, 194, 509, 696, 778, 1359, 1653, 1697
TatI WGTACW 2 cut(s) 871, 1706
TauI GCSGC 3 cut(s) 176, 1973, 2032
TfiI GAWTC 4 cut(s) 155, 207, 409, 991
Tru1I TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
Tru9I TTAA 7 cut(s) 201, 423, 438, 828, 1074, 1419, 1509
TscAI CASTG 3 cut(s) 623, 709, 1687
TseFI GTSAC 1 cut(s) 1049
TseI GCWGC 2 cut(s) 350, 1973
Tsp45I GTSAC 1 cut(s) 1049
TspDTI ATGAA 8 cut(s) 126, 240, 321, 376, 752, 1364, 1657, 1906
TspGWI ACGGA 2 cut(s) 1351, 1970
TspRI CASTG 3 cut(s) 623, 709, 1687
Vha464I CTTAAG 1 cut(s) 1508
VneI GTGCAC 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 1689
XapI RAATTY 3 cut(s) 780, 800, 1126
XbaI TCTAGA 1 cut(s) 1363
XmaJI CCTAGG 1 cut(s) 767
XmiI GTMKAC 2 cut(s) 194, 457
XmnI GAANNNNTTC 1 cut(s) 1812
XspI CTAG 4 cut(s) 768, 813, 1028, 1364
Zsp2I ATGCAT 2 cut(s) 257, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.