Rorug01G0128700

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014826
Physical Location & Seq
Forward (+)
21933980 .. 21935082
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0128700.1

Sequence Viewer

Length: 987 bp
ATGGAATCGGGTGCTTACCCAGTTGGTTACAGATTTCATCCTACCGACCTGGAATTGGTGGCTTACTACCTCCACAACCGGGTTGCCATGGTTGATCAATTTCGCTCGAGTCCAATCCTTGATTGCCCTGATTTCTATGGCAAAAATGAGCCCTGGCTCATTTGGGACATGTTCCGTGCCCAATCCAACAATATGAAAGAGGAAGAAACTCTTGTTATTGGAATTAAGAGAGAATTTCGGTATGAGGGTGGATCTGATCCACATCAAAATGGGGCTTGGTTGATGCAGGAGTACCAGATCACATCTCACAGTAATGATCTCGTACTCTGCACCCTCAGAAAAAACCTCAGAAAACTTCCACCCCCAACTTGTCCTGCTCCTCTGAATCAGAGCCACAATATTATTAGCAACAAAAGGAAGATGAATGAGGAGGCTATAAACACAAAGTCTTTCAAAAGAAAAAAAATGGAACCTCCTAAACAAAAAGAACAACTAATGTTGCCCTCTACCTCTTACTTGTTTGAGCAATCTGAAATATTGTTCAATGATGATCTCTGTTATATGGATGATCAGCAGGAGAGTAATAATGACCAAATGGTTTCTGTTGCTCAAACGTTTGATAGTATTGGTTTAATTCCATTGGTTCAAGTTGAGGAAGAGAGCAATGATGATCTCATGGCTTCATTTGCTCCGTCATGTGTTACTGATGATCAACTTGAGGTTGTTCAGGTTGAGAAGGAGGGAAGTAATGATGATCTCATGGCATCAGCTGATCAACCGTTATTTACTAATGATGATCAGCTTCAAACAGTTCAGGCTGACGGGACTAATATTTTCATTGCTGATTGTCCTAATGATACTTCATATGCCTATTTTGATCCTGAAGTTGAGGCATTCTTTGGCTTGACTACTGATGATATATATGATGATGATGACTGTCAGTTTTCGAGTTCTGAAATACAGGCATTCTTAGCTTGTCTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

328

Amino Acids

37.5

Weight (kDa)

4.24

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 60 1.1e-07 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 614
AclWI GGATC 3 cut(s) 251, 259, 872
AcsI RAATTY 1 cut(s) 233
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 2 cut(s) 293, 324
AfiI CCNNNNNNNGG 2 cut(s) 55, 79
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 4 cut(s) 454, 544, 647, 806
AjnI CCWGG 2 cut(s) 48, 152
AluBI AGCT 3 cut(s) 770, 802, 974
AluI AGCT 3 cut(s) 770, 802, 974
AlwI GGATC 3 cut(s) 251, 259, 872
Ama87I CYCGRG 1 cut(s) 106
ApoI RAATTY 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 181
BanII GRGCYC 1 cut(s) 153
BciT130I CCWGG 2 cut(s) 50, 154
BclI TGATCA 5 cut(s) 94, 568, 709, 772, 796
BcnI CCSGG 1 cut(s) 80
Bme1390I CCNGG 3 cut(s) 50, 80, 154
BmeT110I CYCGRG 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 3 cut(s) 50, 80, 154
BmrI ACTGGG 1 cut(s) 14
BmsI GCATC 2 cut(s) 273, 773
BmuI ACTGGG 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 87, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 79
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 2 cut(s) 670, 837
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 50, 154
BseDI CCNNGG 2 cut(s) 87, 152
BseGI GGATG 2 cut(s) 37, 571
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 55, 79
BseMI GCAATG 2 cut(s) 670, 837
BseMII CTCAG 2 cut(s) 349, 361
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 2 cut(s) 369, 443
BseSI GKGCMC 1 cut(s) 181
BsgI GTGCAG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 2 cut(s) 179, 838
BslI CCNNNNNNNGG 2 cut(s) 55, 79
BsmFI GGGAC 2 cut(s) 179, 838
BsmI GAATGC 2 cut(s) 893, 965
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 153, 181
Bsp19I CCATGG 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 348, 360
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 3 cut(s) 251, 259, 872
BsrDI GCAATG 2 cut(s) 670, 837
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 87, 152
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 50, 154
Bst4CI ACNGT 4 cut(s) 311, 780, 811, 938
Bst6I CTCTTC 1 cut(s) 651
BstDEI CTNAG 3 cut(s) 335, 347, 970
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 37, 571
BstMWI GCNNNNNNNGC 2 cut(s) 686, 971
BstNI CCWGG 2 cut(s) 50, 154
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 3 cut(s) 48, 78, 152
BstSLI GKGCMC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 37, 571
Csp6I GTAC 2 cut(s) 292, 323
CviAII CATG 5 cut(s) 88, 169, 676, 696, 760
CviQI GTAC 2 cut(s) 292, 323
DdeI CTNAG 3 cut(s) 335, 347, 970
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 903
Eco88I CYCGRG 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 48, 152
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 91, 172, 679, 699, 763
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 2 cut(s) 179, 838
FatI CATG 5 cut(s) 87, 168, 675, 695, 759
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 5 cut(s) 94, 568, 709, 772, 796
FokI GGATG 2 cut(s) 24, 578
FriOI GRGCYC 1 cut(s) 153
HapII CCGG 1 cut(s) 79
Hin1II CATG 5 cut(s) 91, 172, 679, 699, 763
HinfI GANTC 3 cut(s) 5, 109, 385
HpaII CCGG 1 cut(s) 79
Hpy188I TCNGA 7 cut(s) 256, 338, 350, 384, 390, 532, 955
Hpy188III TCNNGA 2 cut(s) 881, 980
HpyAV CCTTC 1 cut(s) 730
HpyCH4III ACNGT 4 cut(s) 311, 780, 811, 938
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 2 cut(s) 286, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 971
HpyF3I CTNAG 3 cut(s) 335, 347, 970
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 5 cut(s) 91, 172, 679, 699, 763
Ksp22I TGATCA 5 cut(s) 94, 568, 709, 772, 796
LmnI GCTCC 2 cut(s) 382, 694
LweI GCATC 2 cut(s) 273, 773
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 2 cut(s) 26, 700
MboII GAAGA 3 cut(s) 215, 430, 668
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 153, 181
MluCI AATT 5 cut(s) 53, 98, 222, 233, 633
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 2 cut(s) 225, 632
MslI CAYNNNNRTG 2 cut(s) 267, 312
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 3 cut(s) 50, 80, 154
Mva1269I GAATGC 2 cut(s) 893, 965
MvaI CCWGG 2 cut(s) 50, 154
MwoI GCNNNNNNNGC 2 cut(s) 686, 971
NciI CCSGG 1 cut(s) 80
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 865
NlaIII CATG 5 cut(s) 91, 172, 679, 699, 763
NlaIV GGNNCC 1 cut(s) 471
NspI RCATGY 1 cut(s) 172
PaeR7I CTCGAG 1 cut(s) 106
PciI ACATGT 1 cut(s) 168
PctI GAATGC 2 cut(s) 893, 965
PfeI GAWTC 2 cut(s) 5, 385
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
PscI ACATGT 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 614
Psp6I CCWGG 2 cut(s) 48, 152
PspGI CCWGG 2 cut(s) 48, 152
PspN4I GGNNCC 1 cut(s) 471
PspXI VCTCGAGB 1 cut(s) 106
PsuI RGATCY 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 2 cut(s) 293, 324
RsaNI GTAC 2 cut(s) 292, 323
RseI CAYNNNNRTG 2 cut(s) 267, 312
SaqAI TTAA 2 cut(s) 225, 632
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 3 cut(s) 50, 80, 154
SduI GDGCHC 2 cut(s) 153, 181
SfaNI GCATC 2 cut(s) 273, 773
Sfr274I CTCGAG 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 267, 312
SmlI CTYRAG 2 cut(s) 106, 716
SmoI CTYRAG 2 cut(s) 106, 716
Sse9I AATT 5 cut(s) 53, 98, 222, 233, 633
SspI AATATT 3 cut(s) 400, 537, 832
StyD4I CCNGG 3 cut(s) 48, 78, 152
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 311, 780, 811, 938
TaiI ACGT 1 cut(s) 617
TaqI TCGA 2 cut(s) 107, 947
TasI AATT 5 cut(s) 53, 98, 222, 233, 633
TfiI GAWTC 2 cut(s) 5, 385
Tru1I TTAA 2 cut(s) 225, 632
Tru9I TTAA 2 cut(s) 225, 632
TspDTI ATGAA 6 cut(s) 26, 209, 437, 672, 826, 852
TspGWI ACGGA 2 cut(s) 164, 681
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.