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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 |
| fragaria_vesca | FvH4_7g24020 FvH4_7g24020 FvH4_7g24020 |
| malus_domestica | MD01G1147200.v1.1 |
| prunus_persica | Prupe.2G246100_v2.0.a1 |
| pyrus_communis | pycom01g16620 pycom07g19650 pycom07g19670 |
| rosa_chinensis | RchiOBHm_Chr1g0369361 RchiOBHm_Chr1g0369401 |
| rosa_laevigata | RLG00000027133 |
| rosa_multiflora | Rmu_co8268097.1_g000001 Rmu_sc0003612.1_g000020 Rmu_sc0006332.1_g000003 |
| rosa_roxburghii | Rroxscaffold_4G00287680 |
| rosa_rugosa | Rorug01G0349700 Rorug01G0349800 |
| rosa_samantha | Rh1AG357200 Rh1AG357400 Rh1BG321400 Rh1CG335300 Rh1DG352100 |
| rosa_wichuraiana | Rw1G031540 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 125 |
| Acc36I | ACCTGC | 3 cut(s) | 247, 1444, 1828 |
| Acc65I | GGTACC | 2 cut(s) | 1641, 2060 |
| AccB1I | GGYRCC | 3 cut(s) | 1332, 1641, 2060 |
| AccBSI | CCGCTC | 1 cut(s) | 154 |
| AccII | CGCG | 3 cut(s) | 107, 269, 276 |
| AciI | CCGC | 8 cut(s) | 105, 154, 327, 374, 843, 1115, 1662, 1802 |
| AclWI | GGATC | 7 cut(s) | 735, 832, 853, 1351, 2029, 2034, 2047 |
| AcoI | YGGCCR | 3 cut(s) | 102, 541, 625 |
| AcsI | RAATTY | 3 cut(s) | 86, 985, 1287 |
| AcuI | CTGAAG | 1 cut(s) | 323 |
| AcyI | GRCGYC | 2 cut(s) | 51, 122 |
| AfaI | GTAC | 3 cut(s) | 1361, 1643, 2062 |
| AfeI | AGCGCT | 1 cut(s) | 246 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1638 |
| AgeI | ACCGGT | 1 cut(s) | 5 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1761 |
| AjiI | CACGTC | 1 cut(s) | 782 |
| AjnI | CCWGG | 2 cut(s) | 1073, 1948 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1120, 1152, 1537, 1569 |
| AleI | CACNNNNGTG | 1 cut(s) | 807 |
| AluBI | AGCT | 6 cut(s) | 694, 895, 908, 1023, 1406, 1797 |
| AluI | AGCT | 6 cut(s) | 694, 895, 908, 1023, 1406, 1797 |
| Alw21I | GWGCWC | 2 cut(s) | 587, 680 |
| Alw26I | GTCTC | 3 cut(s) | 677, 1043, 1748 |
| AlwI | GGATC | 7 cut(s) | 735, 832, 853, 1351, 2029, 2034, 2047 |
| Aor51HI | AGCGCT | 1 cut(s) | 246 |
| AoxI | GGCC | 5 cut(s) | 102, 541, 625, 1547, 1657 |
| ApeKI | GCWGC | 7 cut(s) | 199, 202, 946, 1768, 1925, 1989, 1992 |
| ApoI | RAATTY | 3 cut(s) | 86, 985, 1287 |
| AsiGI | ACCGGT | 1 cut(s) | 5 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 24 |
| Asp718I | GGTACC | 2 cut(s) | 1641, 2060 |
| AspLEI | GCGC | 3 cut(s) | 247, 269, 278 |
| AspS9I | GGNCC | 2 cut(s) | 220, 1658 |
| AsuC2I | CCSGG | 2 cut(s) | 224, 1341 |
| AsuHPI | GGTGA | 4 cut(s) | 553, 794, 1131, 1791 |
| AsuII | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| AsuNHI | GCTAGC | 1 cut(s) | 388 |
| AvaII | GGWCC | 1 cut(s) | 220 |
| AxyI | CCTNAGG | 1 cut(s) | 1061 |
| BaeGI | GKGCMC | 2 cut(s) | 464, 1337 |
| BalI | TGGCCA | 1 cut(s) | 543 |
| BamHI | GGATCC | 1 cut(s) | 2039 |
| BanI | GGYRCC | 3 cut(s) | 1332, 1641, 2060 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| Bbv12I | GWGCWC | 2 cut(s) | 587, 680 |
| BbvI | GCAGC | 7 cut(s) | 186, 214, 933, 1755, 1937, 2001, 2004 |
| BccI | CCATC | 4 cut(s) | 533, 647, 908, 1801 |
| BceAI | ACGGC | 2 cut(s) | 64, 1857 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 972, 1006 |
| BciT130I | CCWGG | 2 cut(s) | 1075, 1950 |
| BciVI | GTATCC | 1 cut(s) | 159 |
| BclI | TGATCA | 1 cut(s) | 732 |
| BcnI | CCSGG | 2 cut(s) | 224, 1341 |
| BcoDI | GTCTC | 3 cut(s) | 677, 1043, 1748 |
| BfaI | CTAG | 2 cut(s) | 389, 2025 |
| BfmI | CTRYAG | 2 cut(s) | 200, 591 |
| BfoI | RGCGCY | 1 cut(s) | 248 |
| BfuAI | ACCTGC | 3 cut(s) | 247, 1444, 1828 |
| BfuI | GTATCC | 1 cut(s) | 159 |
| BglII | AGATCT | 1 cut(s) | 190 |
| BisI | GCNGC | 9 cut(s) | 105, 200, 203, 327, 947, 1769, 1926, 1990, 1993 |
| BlsI | GCNGC | 9 cut(s) | 106, 201, 204, 328, 948, 1770, 1927, 1991, 1994 |
| BmcAI | AGTACT | 1 cut(s) | 1361 |
| Bme1390I | CCNGG | 4 cut(s) | 224, 1075, 1341, 1950 |
| Bme18I | GGWCC | 1 cut(s) | 220 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1761 |
| BmgBI | CACGTC | 1 cut(s) | 782 |
| BmgT120I | GGNCC | 2 cut(s) | 220, 1658 |
| BmiI | GGNNCC | 7 cut(s) | 1334, 1643, 1660, 1871, 1891, 2041, 2062 |
| BmrFI | CCNGG | 4 cut(s) | 224, 1075, 1341, 1950 |
| BmrI | ACTGGG | 1 cut(s) | 574 |
| BmsI | GCATC | 3 cut(s) | 313, 1166, 1289 |
| BmtI | GCTAGC | 1 cut(s) | 392 |
| BmuI | ACTGGG | 1 cut(s) | 574 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| Bpu14I | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| BpuEI | CTTGAG | 1 cut(s) | 894 |
| BpuMI | CCSGG | 2 cut(s) | 224, 1341 |
| BsaHI | GRCGYC | 2 cut(s) | 51, 122 |
| BsaI | GGTCTC | 1 cut(s) | 1043 |
| BsaJI | CCNNGG | 6 cut(s) | 81, 1013, 1074, 1544, 1873, 1949 |
| BsaWI | WCCGGW | 3 cut(s) | 5, 1536, 1631 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1861, 1891 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1638 |
| Bse118I | RCCGGY | 2 cut(s) | 5, 627 |
| Bse1I | ACTGG | 8 cut(s) | 174, 580, 769, 1142, 1440, 1678, 1891, 1966 |
| Bse21I | CCTNAGG | 1 cut(s) | 1061 |
| Bse3DI | GCAATG | 2 cut(s) | 1920, 2002 |
| BseBI | CCWGG | 2 cut(s) | 1075, 1950 |
| BseDI | CCNNGG | 6 cut(s) | 81, 1013, 1074, 1544, 1873, 1949 |
| BseGI | GGATG | 8 cut(s) | 506, 541, 1055, 1391, 1533, 1816, 2020, 2088 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1638 |
| BseMI | GCAATG | 2 cut(s) | 1920, 2002 |
| BseMII | CTCAG | 6 cut(s) | 362, 371, 672, 998, 1052, 1571 |
| BseNI | ACTGG | 8 cut(s) | 174, 580, 769, 1142, 1440, 1678, 1891, 1966 |
| BseRI | GAGGAG | 2 cut(s) | 1102, 1296 |
| BseSI | GKGCMC | 2 cut(s) | 464, 1337 |
| BseX3I | CGGCCG | 1 cut(s) | 102 |
| BseXI | GCAGC | 7 cut(s) | 186, 214, 933, 1755, 1937, 2001, 2004 |
| BseYI | CCCAGC | 1 cut(s) | 1865 |
| BsgI | GTGCAG | 1 cut(s) | 840 |
| Bsh1236I | CGCG | 3 cut(s) | 107, 269, 276 |
| Bsh1285I | CGRYCG | 4 cut(s) | 58, 70, 105, 1119 |
| BshFI | GGCC | 5 cut(s) | 104, 543, 627, 1549, 1659 |
| BshNI | GGYRCC | 3 cut(s) | 1332, 1641, 2060 |
| BshTI | ACCGGT | 1 cut(s) | 5 |
| BsiEI | CGRYCG | 4 cut(s) | 58, 70, 105, 1119 |
| BsiHKAI | GWGCWC | 2 cut(s) | 587, 680 |
| BsiSI | CCGG | 7 cut(s) | 6, 223, 628, 1341, 1386, 1537, 1632 |
| BslFI | GGGAC | 4 cut(s) | 197, 1112, 1300, 1479 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1638 |
| BsmAI | GTCTC | 3 cut(s) | 677, 1043, 1748 |
| BsmFI | GGGAC | 4 cut(s) | 197, 1112, 1300, 1479 |
| BsmI | GAATGC | 3 cut(s) | 316, 390, 1677 |
| BsnI | GGCC | 5 cut(s) | 104, 543, 627, 1549, 1659 |
| Bso31I | GGTCTC | 1 cut(s) | 1043 |
| Bsp119I | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| Bsp1286I | GDGCHC | 4 cut(s) | 464, 587, 680, 1337 |
| BspACI | CCGC | 8 cut(s) | 105, 154, 327, 374, 843, 1115, 1662, 1802 |
| BspANI | GGCC | 5 cut(s) | 104, 543, 627, 1549, 1659 |
| BspCNI | CTCAG | 6 cut(s) | 361, 370, 673, 999, 1053, 1570 |
| BspFNI | CGCG | 3 cut(s) | 107, 269, 276 |
| BspLI | GGNNCC | 7 cut(s) | 1334, 1643, 1660, 1871, 1891, 2041, 2062 |
| BspMAI | CTGCAG | 1 cut(s) | 204 |
| BspMI | ACCTGC | 3 cut(s) | 247, 1444, 1828 |
| BspOI | GCTAGC | 1 cut(s) | 392 |
| BspPI | GGATC | 7 cut(s) | 735, 832, 853, 1351, 2029, 2034, 2047 |
| BspQI | GCTCTTC | 2 cut(s) | 144, 1633 |
| BspT104I | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| BspT107I | GGYRCC | 3 cut(s) | 1332, 1641, 2060 |
| BspTNI | GGTCTC | 1 cut(s) | 1043 |
| BsrBI | CCGCTC | 1 cut(s) | 154 |
| BsrDI | GCAATG | 2 cut(s) | 1920, 2002 |
| BsrFI | RCCGGY | 2 cut(s) | 5, 627 |
| BsrI | ACTGG | 8 cut(s) | 174, 580, 769, 1142, 1440, 1678, 1891, 1966 |
| BssAI | RCCGGY | 2 cut(s) | 5, 627 |
| BssECI | CCNNGG | 6 cut(s) | 81, 1013, 1074, 1544, 1873, 1949 |
| BssNI | GRCGYC | 2 cut(s) | 51, 122 |
| BssT1I | CCWWGG | 2 cut(s) | 1013, 1544 |
| Bst2UI | CCWGG | 2 cut(s) | 1075, 1950 |
| Bst4CI | ACNGT | 5 cut(s) | 496, 832, 1258, 1328, 1972 |
| Bst6I | CTCTTC | 2 cut(s) | 144, 1633 |
| BstACI | GRCGYC | 2 cut(s) | 51, 122 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1154 |
| BstBI | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| BstC8I | GCNNGC | 4 cut(s) | 345, 372, 390, 1657 |
| BstDEI | CTNAG | 6 cut(s) | 348, 357, 681, 1007, 1061, 1557 |
| BstDSI | CCRYGG | 1 cut(s) | 1873 |
| BstF5I | GGATG | 8 cut(s) | 506, 541, 1055, 1391, 1533, 1816, 2020, 2088 |
| BstFNI | CGCG | 3 cut(s) | 107, 269, 276 |
| BstH2I | RGCGCY | 1 cut(s) | 248 |
| BstHHI | GCGC | 3 cut(s) | 247, 269, 278 |
| BstMAI | GTCTC | 3 cut(s) | 677, 1043, 1748 |
| BstMCI | CGRYCG | 4 cut(s) | 58, 70, 105, 1119 |
| BstNI | CCWGG | 2 cut(s) | 1075, 1950 |
| BstSCI | CCNGG | 4 cut(s) | 222, 1073, 1339, 1948 |
| BstSFI | CTRYAG | 2 cut(s) | 200, 591 |
| BstSLI | GKGCMC | 2 cut(s) | 464, 1337 |
| BstUI | CGCG | 3 cut(s) | 107, 269, 276 |
| BstV1I | GCAGC | 7 cut(s) | 186, 214, 933, 1755, 1937, 2001, 2004 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BstX2I | RGATCY | 4 cut(s) | 190, 727, 2021, 2039 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1936 |
| BstYI | RGATCY | 4 cut(s) | 190, 727, 2021, 2039 |
| BstZI | CGGCCG | 1 cut(s) | 102 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1061 |
| BsuI | GTATCC | 1 cut(s) | 159 |
| BsuRI | GGCC | 5 cut(s) | 104, 543, 627, 1549, 1659 |
| BtgI | CCRYGG | 1 cut(s) | 1873 |
| BtrI | CACGTC | 1 cut(s) | 782 |
| BtsCI | GGATG | 8 cut(s) | 506, 541, 1055, 1391, 1533, 1816, 2020, 2088 |
| BtsI | GCAGTG | 2 cut(s) | 239, 1943 |
| BtsIMutI | CAGTG | 7 cut(s) | 239, 587, 837, 1149, 1657, 1884, 1943 |
| BveI | ACCTGC | 3 cut(s) | 247, 1444, 1828 |
| Cac8I | GCNNGC | 4 cut(s) | 345, 372, 390, 1657 |
| CfoI | GCGC | 3 cut(s) | 247, 269, 278 |
| Cfr10I | RCCGGY | 2 cut(s) | 5, 627 |
| Cfr13I | GGNCC | 2 cut(s) | 220, 1658 |
| CseI | GACGC | 2 cut(s) | 40, 1751 |
| Csp6I | GTAC | 3 cut(s) | 1360, 1642, 2061 |
| CspAI | ACCGGT | 1 cut(s) | 5 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1464, 1499 |
| CviAII | CATG | 3 cut(s) | 1297, 1825, 1930 |
| CviQI | GTAC | 3 cut(s) | 1360, 1642, 2061 |
| DdeI | CTNAG | 6 cut(s) | 348, 357, 681, 1007, 1061, 1557 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1761 |
| EaeI | YGGCCR | 3 cut(s) | 102, 541, 625 |
| EagI | CGGCCG | 1 cut(s) | 102 |
| Eam1104I | CTCTTC | 2 cut(s) | 144, 1633 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1761 |
| EarI | CTCTTC | 2 cut(s) | 144, 1633 |
| EclXI | CGGCCG | 1 cut(s) | 102 |
| Eco130I | CCWWGG | 2 cut(s) | 1013, 1544 |
| Eco31I | GGTCTC | 1 cut(s) | 1043 |
| Eco32I | GATATC | 1 cut(s) | 1956 |
| Eco47I | GGWCC | 1 cut(s) | 220 |
| Eco47III | AGCGCT | 1 cut(s) | 246 |
| Eco52I | CGGCCG | 1 cut(s) | 102 |
| Eco57I | CTGAAG | 1 cut(s) | 323 |
| Eco81I | CCTNAGG | 1 cut(s) | 1061 |
| EcoRII | CCWGG | 2 cut(s) | 1073, 1948 |
| EcoRV | GATATC | 1 cut(s) | 1956 |
| EcoT14I | CCWWGG | 2 cut(s) | 1013, 1544 |
| EcoT22I | ATGCAT | 1 cut(s) | 1159 |
| ErhI | CCWWGG | 2 cut(s) | 1013, 1544 |
| FaeI | CATG | 3 cut(s) | 1300, 1828, 1933 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1537, 1569, 1629, 1661 |
| FaqI | GGGAC | 4 cut(s) | 197, 1112, 1300, 1479 |
| FatI | CATG | 3 cut(s) | 1296, 1824, 1929 |
| FauI | CCCGC | 1 cut(s) | 1669 |
| FbaI | TGATCA | 1 cut(s) | 732 |
| Fnu4HI | GCNGC | 9 cut(s) | 105, 200, 203, 327, 947, 1769, 1926, 1990, 1993 |
| FokI | GGATG | 8 cut(s) | 493, 548, 1042, 1378, 1520, 1823, 2027, 2095 |
| Fsp4HI | GCNGC | 9 cut(s) | 105, 200, 203, 327, 947, 1769, 1926, 1990, 1993 |
| FspBI | CTAG | 2 cut(s) | 389, 2025 |
| GlaI | GCGC | 3 cut(s) | 246, 268, 277 |
| GluI | GCNGC | 9 cut(s) | 105, 200, 203, 327, 947, 1769, 1926, 1990, 1993 |
| GsaI | CCCAGC | 1 cut(s) | 1869 |
| HaeII | RGCGCY | 1 cut(s) | 248 |
| HaeIII | GGCC | 5 cut(s) | 104, 543, 627, 1549, 1659 |
| HapII | CCGG | 7 cut(s) | 6, 223, 628, 1341, 1386, 1537, 1632 |
| HgaI | GACGC | 2 cut(s) | 40, 1751 |
| HhaI | GCGC | 3 cut(s) | 247, 269, 278 |
| Hin1I | GRCGYC | 2 cut(s) | 51, 122 |
| Hin1II | CATG | 3 cut(s) | 1300, 1828, 1933 |
| Hin6I | GCGC | 3 cut(s) | 245, 267, 276 |
| HinP1I | GCGC | 3 cut(s) | 245, 267, 276 |
| HincII | GTYRAC | 5 cut(s) | 159, 354, 762, 930, 1461 |
| HindII | GTYRAC | 5 cut(s) | 159, 354, 762, 930, 1461 |
| HindIII | AAGCTT | 1 cut(s) | 692 |
| HpaI | GTTAAC | 1 cut(s) | 762 |
| HpaII | CCGG | 7 cut(s) | 6, 223, 628, 1341, 1386, 1537, 1632 |
| HphI | GGTGA | 4 cut(s) | 553, 794, 1131, 1791 |
| Hpy166II | GTNNAC | 6 cut(s) | 159, 354, 762, 930, 1461, 1603 |
| Hpy188I | TCNGA | 5 cut(s) | 67, 511, 682, 732, 1177 |
| Hpy188III | TCNNGA | 5 cut(s) | 736, 911, 954, 1235, 1523 |
| Hpy8I | GTNNAC | 6 cut(s) | 159, 354, 762, 930, 1461, 1603 |
| Hpy99I | CGWCG | 9 cut(s) | 41, 50, 56, 103, 124, 127, 130, 1767, 1901 |
| HpyAV | CCTTC | 8 cut(s) | 170, 326, 718, 815, 973, 1582, 1735, 2074 |
| HpyCH4III | ACNGT | 5 cut(s) | 496, 832, 1258, 1328, 1972 |
| HpyCH4IV | ACGT | 4 cut(s) | 122, 517, 781, 1899 |
| HpyF3I | CTNAG | 6 cut(s) | 348, 357, 681, 1007, 1061, 1557 |
| HpySE526I | ACGT | 4 cut(s) | 122, 517, 781, 1899 |
| Hsp92I | GRCGYC | 2 cut(s) | 51, 122 |
| Hsp92II | CATG | 3 cut(s) | 1300, 1828, 1933 |
| HspAI | GCGC | 3 cut(s) | 245, 267, 276 |
| KpnI | GGTACC | 2 cut(s) | 1645, 2064 |
| Ksp22I | TGATCA | 1 cut(s) | 732 |
| KspAI | GTTAAC | 1 cut(s) | 762 |
| LguI | GCTCTTC | 2 cut(s) | 144, 1633 |
| LmnI | GCTCC | 3 cut(s) | 116, 892, 1869 |
| Lsp1109I | GCAGC | 7 cut(s) | 186, 214, 933, 1755, 1937, 2001, 2004 |
| LweI | GCATC | 3 cut(s) | 313, 1166, 1289 |
| MaeI | CTAG | 2 cut(s) | 389, 2025 |
| MaeII | ACGT | 4 cut(s) | 122, 517, 781, 1899 |
| MaeIII | GTNAC | 1 cut(s) | 777 |
| MbiI | CCGCTC | 1 cut(s) | 154 |
| MboII | GAAGA | 9 cut(s) | 32, 161, 316, 730, 1212, 1416, 1511, 1620, 2063 |
| MflI | RGATCY | 4 cut(s) | 190, 727, 2021, 2039 |
| MhlI | GDGCHC | 4 cut(s) | 464, 587, 680, 1337 |
| MlsI | TGGCCA | 1 cut(s) | 543 |
| MluNI | TGGCCA | 1 cut(s) | 543 |
| MlyI | GAGTC | 5 cut(s) | 89, 349, 785, 1018, 1750 |
| MmeI | TCCRAC | 6 cut(s) | 361, 409, 817, 1285, 1482, 1716 |
| Mox20I | TGGCCA | 1 cut(s) | 543 |
| Mph1103I | ATGCAT | 1 cut(s) | 1159 |
| MroXI | GAANNNNTTC | 1 cut(s) | 24 |
| MscI | TGGCCA | 1 cut(s) | 543 |
| MseI | TTAA | 4 cut(s) | 566, 761, 876, 1479 |
| MslI | CAYNNNNRTG | 2 cut(s) | 807, 1934 |
| Msp20I | TGGCCA | 1 cut(s) | 543 |
| MspA1I | CMGCKG | 3 cut(s) | 374, 1115, 1804 |
| MspI | CCGG | 7 cut(s) | 6, 223, 628, 1341, 1386, 1537, 1632 |
| MspR9I | CCNGG | 4 cut(s) | 224, 1075, 1341, 1950 |
| Mva1269I | GAATGC | 3 cut(s) | 316, 390, 1677 |
| MvaI | CCWGG | 2 cut(s) | 1075, 1950 |
| MvnI | CGCG | 3 cut(s) | 107, 269, 276 |
| NciI | CCSGG | 2 cut(s) | 224, 1341 |
| NheI | GCTAGC | 1 cut(s) | 388 |
| NlaIII | CATG | 3 cut(s) | 1300, 1828, 1933 |
| NlaIV | GGNNCC | 7 cut(s) | 1334, 1643, 1660, 1871, 1891, 2041, 2062 |
| NmeAIII | GCCGAG | 2 cut(s) | 106, 781 |
| NmuCI | GTSAC | 1 cut(s) | 777 |
| NsiI | ATGCAT | 1 cut(s) | 1159 |
| NspV | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| OliI | CACNNNNGTG | 1 cut(s) | 807 |
| PciSI | GCTCTTC | 2 cut(s) | 144, 1633 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 36, 42, 51, 104 |
| PctI | GAATGC | 3 cut(s) | 316, 390, 1677 |
| PdmI | GAANNNNTTC | 1 cut(s) | 24 |
| PflFI | GACNNNGTC | 2 cut(s) | 50, 124 |
| PfoI | TCCNGGA | 1 cut(s) | 1339 |
| PinAI | ACCGGT | 1 cut(s) | 5 |
| PkrI | GCNGC | 9 cut(s) | 106, 201, 204, 328, 948, 1770, 1927, 1991, 1994 |
| Ple19I | CGATCG | 2 cut(s) | 58, 70 |
| PleI | GAGTC | 5 cut(s) | 89, 349, 784, 1017, 1750 |
| PpsI | GAGTC | 5 cut(s) | 89, 349, 784, 1017, 1750 |
| Psp6I | CCWGG | 2 cut(s) | 1073, 1948 |
| PspFI | CCCAGC | 1 cut(s) | 1865 |
| PspGI | CCWGG | 2 cut(s) | 1073, 1948 |
| PspN4I | GGNNCC | 7 cut(s) | 1334, 1643, 1660, 1871, 1891, 2041, 2062 |
| PspPI | GGNCC | 2 cut(s) | 220, 1658 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2053, 2085 |
| PstI | CTGCAG | 1 cut(s) | 204 |
| PsuI | RGATCY | 4 cut(s) | 190, 727, 2021, 2039 |
| PsyI | GACNNNGTC | 2 cut(s) | 50, 124 |
| PvuI | CGATCG | 2 cut(s) | 58, 70 |
| RsaI | GTAC | 3 cut(s) | 1361, 1643, 2062 |
| RsaNI | GTAC | 3 cut(s) | 1360, 1642, 2061 |
| RseI | CAYNNNNRTG | 2 cut(s) | 807, 1934 |
| SapI | GCTCTTC | 2 cut(s) | 144, 1633 |
| SaqAI | TTAA | 4 cut(s) | 566, 761, 876, 1479 |
| SatI | GCNGC | 9 cut(s) | 105, 200, 203, 327, 947, 1769, 1926, 1990, 1993 |
| Sau96I | GGNCC | 2 cut(s) | 220, 1658 |
| ScaI | AGTACT | 1 cut(s) | 1361 |
| SchI | GAGTC | 5 cut(s) | 89, 349, 785, 1018, 1750 |
| ScrFI | CCNGG | 4 cut(s) | 224, 1075, 1341, 1950 |
| SduI | GDGCHC | 4 cut(s) | 464, 587, 680, 1337 |
| SfaNI | GCATC | 3 cut(s) | 313, 1166, 1289 |
| SfcI | CTRYAG | 2 cut(s) | 200, 591 |
| SfuI | TTCGAA | 3 cut(s) | 317, 716, 2072 |
| SinI | GGWCC | 1 cut(s) | 220 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 807, 1934 |
| SmlI | CTYRAG | 1 cut(s) | 909 |
| SmoI | CTYRAG | 1 cut(s) | 909 |
| SsiI | CCGC | 8 cut(s) | 105, 154, 327, 374, 843, 1115, 1662, 1802 |
| SspMI | CTAG | 2 cut(s) | 389, 2025 |
| StyD4I | CCNGG | 4 cut(s) | 222, 1073, 1339, 1948 |
| StyI | CCWWGG | 2 cut(s) | 1013, 1544 |
| TaaI | ACNGT | 5 cut(s) | 496, 832, 1258, 1328, 1972 |
| TaiI | ACGT | 4 cut(s) | 125, 520, 784, 1902 |
| TaqI | TCGA | 9 cut(s) | 45, 54, 317, 716, 955, 1119, 1595, 1694, 2072 |
| TaqII | GACCGA | 1 cut(s) | 60 |
| TatI | WGTACW | 1 cut(s) | 1359 |
| TauI | GCSGC | 2 cut(s) | 107, 329 |
| Tru1I | TTAA | 4 cut(s) | 566, 761, 876, 1479 |
| Tru9I | TTAA | 4 cut(s) | 566, 761, 876, 1479 |
| TscAI | CASTG | 7 cut(s) | 239, 587, 837, 1149, 1657, 1891, 1950 |
| TseFI | GTSAC | 1 cut(s) | 777 |
| TseI | GCWGC | 7 cut(s) | 199, 202, 946, 1768, 1925, 1989, 1992 |
| Tsp45I | GTSAC | 1 cut(s) | 777 |
| TspDTI | ATGAA | 9 cut(s) | 323, 513, 625, 737, 978, 992, 1212, 1718, 2099 |
| TspGWI | ACGGA | 1 cut(s) | 201 |
| TspRI | CASTG | 7 cut(s) | 239, 587, 837, 1149, 1657, 1891, 1950 |
| Tth111I | GACNNNGTC | 2 cut(s) | 50, 124 |
| VpaK11BI | GGWCC | 1 cut(s) | 220 |
| XapI | RAATTY | 3 cut(s) | 86, 985, 1287 |
| XmnI | GAANNNNTTC | 1 cut(s) | 24 |
| XspI | CTAG | 2 cut(s) | 389, 2025 |
| ZraI | GACGTC | 1 cut(s) | 123 |
| ZrmI | AGTACT | 1 cut(s) | 1361 |
| Zsp2I | ATGCAT | 1 cut(s) | 1159 |