Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0004441

Physical Location & Seq

Genomic Coordinates Forward (+)

8665102 .. 8670933

Gene Structure 5832 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00287680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGACCGGCCATAGAAACGAAGACTTTCACGCTAACGACGAGATCGACGGCGCCGATCGCATTTCCGATCGGTCAAACGCCGAGGAATTCGAGGACTCGTCGGCCGCGAACGAGGTCGGGAGCTCGGGCGAGGGAGCGACGTCGTCGGGGAGTGAGATTGGGCTGGAAGAGCGGTTGACAGGGATACTGGTTGAAGGAGGGGACGGAGATCTGTTGCTGCAGCAGAGCAACCGAGAGGACCGGGTTTTGCAGTGGCTTCAAGCGCTTGATATGCAGGTTATGGGCGCGTGTCGCGCAGACGAGAGGTTGAAGCCTTTGCTGAAGATGAATGCTTCGAACGATGCCGCAGAAGGTCGGTTGCTGGCTCAGTTGACTCAGCATTTTGAGCCAGCGGAAGTTGGAATGCTAGCGAGGTGCTTTTGCATACCACTTGTTTCTGTTCGTGTTGGAAAGATCAACAAGCAAGGGATTTTGTTGTACCCTACCAATTCAAAGGGTAATTTGAATCTTACAGTCTTGCCAACATCCGATCTACGTCTTTCATTTGTTGGGGATGATGGCCATACAGATAGACTATTCACCTTAAATAGCAAATCCCAGTGCTCTGCTGTAGAAGTCAATGAGATTCCAGCAGACAATTCTGGCCGGTCTTTCATTATCAAAATCCCAGATGGTCGGGTTTTCCACTTTTGGTGCGCTGAGACATCAAAGCTTTTGGGAATTGAATTGATTTCAAAGATGAAGGATCTGATCAGGAGGAAGCCCTCTATTGCCGAGTTAACTGGAATCAGCGAGTCACGTCTTGGTTGCTTTGCAACTCACCTTCGTGCCTATCTGGTGGGATCAACAGTGGTTGGAAGCGGATCAAGTTCTGCACCCTCAAATACTGATGTTGATACCTCCAATATGGAGCTATTTGATACAGCTCAAGACGGACAACTCTCGTCAACATCATCAAAGTCGCTGCGTTCTCGACATAGTGTGAATCAGTCAATGAAGGCAAATTCATCGTTTCAGGGTAGCCTGAGTCCAAGGTCAAGCTCTTTCAAAGAGGGTCTCCCCAGAACCTTGTCGTCCCTGAGGAATATAACCAGGGAAAAGTTGAGGAGGCGAGGGGACATTTATCTTTCAGCGGTCGATGACCCAACAATCGTTTCAGCAGTGGCAATCAATGCATCTTGTTCAAATCAATCTGAAAATGACAAGTGTGCAGAAGTTGTCGCAAGTTGCTCATTATCTTCATCAAGTTTTCTGGAGTCACTTGGAAAATTGACTGTCCAACCAACCCTGAACTCTGCATCTCAAATTCCATACATGGCTACTCCTCTTTTGTCCCCCTACTACTGTTGGTGCCCTCCCGGATCATCAGGTTTGCAGTACTCACAAGAACCTCCTGCAATCTCCAGCTCATCCATTGAATCAGCTTTGCTTCCACCACTTTCTTCTTTAATACCTGCCAGTATGCCCTCCTGTATGTTGACAGCAAATCCTCCACTTAATTTGGCAGATTGTCCCTTGTTGGATTTCCCTGCCTTTCTTCCAGACCCACTCATCCGGTTGCCAAGGCCAACTTCTCAGCAGATCCCAACCTTCAATCCTTTGATTTGTGATCCGATTGTTCACATTCCTGTTATGGATATTTGCTCTTCCGGTCAAGGGTACCTTGTCAGTGCTGGCCCCGCTATTTCAACTGGCATTCCTCCCTTGCACTCAAACCTTATGAATCCATTGATTCCTCAAACTGATTCTATGTTGGAGAAGGGTGCTAGAGAGACTCTGCGTCGGCTGCTCATAAGTGGTTCAACCCAGAGTAGCTCACCACTGATGGATGTATTACCTGCCATGCTAACAAATGCAGATGAGAATAGAAATATGCTTGTTGCTGGGAGCCGTGGTCTATACACTGGAACCAGCGACGTTGATGTAATTGCAAATAGCATTGCAGCCATGAGTATGGTTTCACTGCCTGGGATATCTACTGGTGGAACTGTTTTGGAGAATTGTGGCAGCAGCAATGGTTTTGACATACAGGATGAGGGATCTAGTGGATTGGGTGGATCCTGTTTGGAAGATCAGGGTACCTTCTGTTCGAATTATGGGATGAAAAGGACTGATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

706

Amino Acids

75.35

Weight (kDa)

4.92

Isoelectric Point (pI)

49.13

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004441)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950 AT2G39950
fragaria_vesca	FvH4_7g24020 FvH4_7g24020 FvH4_7g24020
malus_domestica	MD01G1147200.v1.1
prunus_persica	Prupe.2G246100_v2.0.a1
pyrus_communis	pycom01g16620 pycom07g19650 pycom07g19670
rosa_chinensis	RchiOBHm_Chr1g0369361 RchiOBHm_Chr1g0369401
rosa_laevigata	RLG00000027133
rosa_multiflora	Rmu_co8268097.1_g000001 Rmu_sc0003612.1_g000020 Rmu_sc0006332.1_g000003
rosa_roxburghii	Rroxscaffold_4G00287680
rosa_rugosa	Rorug01G0349700 Rorug01G0349800
rosa_samantha	Rh1AG357200 Rh1AG357400 Rh1BG321400 Rh1CG335300 Rh1DG352100
rosa_wichuraiana	Rw1G031540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	143
Acc36I	ACCTGC	3 cut(s)	265, 1462, 1846
Acc65I	GGTACC	2 cut(s)	1659, 2078
AccB1I	GGYRCC	4 cut(s)	50, 1350, 1659, 2078
AccBSI	CCGCTC	1 cut(s)	172
AccII	CGCG	3 cut(s)	107, 287, 294
AciI	CCGC	7 cut(s)	105, 172, 345, 392, 861, 1133, 1680
AclWI	GGATC	9 cut(s)	753, 850, 871, 1369, 1576, 1604, 2047, 2052, 2065
AcoI	YGGCCR	4 cut(s)	7, 102, 559, 643
AcsI	RAATTY	3 cut(s)	86, 1003, 1305
AcuI	CTGAAG	1 cut(s)	341
AcyI	GRCGYC	2 cut(s)	51, 140
AfaI	GTAC	4 cut(s)	479, 1379, 1661, 2080
AfeI	AGCGCT	1 cut(s)	264
AfiI	CCNNNNNNNGG	1 cut(s)	1656
AhdI	GACNNNNNGTC	1 cut(s)	1779
AjiI	CACGTC	1 cut(s)	800
AjnI	CCWGG	2 cut(s)	1091, 1966
AjuI	GAANNNNNNNTTGG	4 cut(s)	1138, 1170, 1555, 1587
AleI	CACNNNNGTG	1 cut(s)	825
AluBI	AGCT	8 cut(s)	123, 712, 913, 926, 1041, 1407, 1424, 1815
AluI	AGCT	8 cut(s)	123, 712, 913, 926, 1041, 1407, 1424, 1815
Alw21I	GWGCWC	2 cut(s)	125, 605
Alw26I	GTCTC	3 cut(s)	695, 1061, 1766
AlwI	GGATC	9 cut(s)	753, 850, 871, 1369, 1576, 1604, 2047, 2052, 2065
Ama87I	CYCGRG	1 cut(s)	124
Aor51HI	AGCGCT	1 cut(s)	264
AoxI	GGCC	6 cut(s)	7, 102, 559, 643, 1565, 1675
ApeKI	GCWGC	7 cut(s)	217, 220, 964, 1786, 1943, 2007, 2010
ApoI	RAATTY	3 cut(s)	86, 1003, 1305
Asp700I	GAANNNNTTC	1 cut(s)	24
Asp718I	GGTACC	2 cut(s)	1659, 2078
AspLEI	GCGC	5 cut(s)	53, 265, 287, 296, 698
AspS9I	GGNCC	2 cut(s)	238, 1676
AsuC2I	CCSGG	2 cut(s)	242, 1359
AsuHPI	GGTGA	3 cut(s)	571, 812, 1809
AsuII	TTCGAA	2 cut(s)	335, 2090
AsuNHI	GCTAGC	1 cut(s)	406
AvaI	CYCGRG	1 cut(s)	124
AvaII	GGWCC	1 cut(s)	238
AxyI	CCTNAGG	1 cut(s)	1079
BaeGI	GKGCMC	1 cut(s)	1355
BalI	TGGCCA	1 cut(s)	561
BamHI	GGATCC	1 cut(s)	2057
BanI	GGYRCC	4 cut(s)	50, 1350, 1659, 2078
BanII	GRGCYC	1 cut(s)	125
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	2 cut(s)	125, 605
BbvI	GCAGC	7 cut(s)	204, 232, 951, 1773, 1955, 2019, 2022
BccI	CCATC	3 cut(s)	551, 665, 1819
BceAI	ACGGC	2 cut(s)	64, 1875
BcgI	CGANNNNNNTGC	2 cut(s)	990, 1024
BciT130I	CCWGG	2 cut(s)	1093, 1968
BciVI	GTATCC	1 cut(s)	177
BclI	TGATCA	1 cut(s)	750
BcnI	CCSGG	2 cut(s)	242, 1359
BcoDI	GTCTC	3 cut(s)	695, 1061, 1766
BfaI	CTAG	3 cut(s)	407, 1767, 2043
BfmI	CTRYAG	2 cut(s)	218, 609
BfoI	RGCGCY	2 cut(s)	54, 266
BfuAI	ACCTGC	3 cut(s)	265, 1462, 1846
BfuI	GTATCC	1 cut(s)	177
BglII	AGATCT	1 cut(s)	208
BisI	GCNGC	9 cut(s)	105, 218, 221, 345, 965, 1787, 1944, 2008, 2011
BlsI	GCNGC	9 cut(s)	106, 219, 222, 346, 966, 1788, 1945, 2009, 2012
BmcAI	AGTACT	1 cut(s)	1379
Bme1390I	CCNGG	4 cut(s)	242, 1093, 1359, 1968
Bme18I	GGWCC	1 cut(s)	238
BmeRI	GACNNNNNGTC	1 cut(s)	1779
BmeT110I	CYCGRG	1 cut(s)	124
BmgBI	CACGTC	1 cut(s)	800
BmgT120I	GGNCC	2 cut(s)	238, 1676
BmiI	GGNNCC	8 cut(s)	52, 1352, 1661, 1678, 1889, 1909, 2059, 2080
BmrFI	CCNGG	4 cut(s)	242, 1093, 1359, 1968
BmrI	ACTGGG	1 cut(s)	592
BmsI	GCATC	3 cut(s)	331, 1184, 1307
BmtI	GCTAGC	1 cut(s)	410
BmuI	ACTGGG	1 cut(s)	592
BpiI	GAAGAC	1 cut(s)	27
BpmI	CTGGAG	2 cut(s)	1274, 1387
Bpu14I	TTCGAA	2 cut(s)	335, 2090
BpuEI	CTTGAG	1 cut(s)	912
BpuMI	CCSGG	2 cut(s)	242, 1359
BsaHI	GRCGYC	2 cut(s)	51, 140
BsaI	GGTCTC	1 cut(s)	1061
BsaJI	CCNNGG	6 cut(s)	81, 1031, 1092, 1562, 1891, 1967
BsaWI	WCCGGW	2 cut(s)	1554, 1649
BsaXI	ACNNNNNCTCC	2 cut(s)	1879, 1909
Bsc4I	CCNNNNNNNGG	1 cut(s)	1656
Bse118I	RCCGGY	2 cut(s)	5, 645
Bse1I	ACTGG	7 cut(s)	192, 598, 787, 1458, 1696, 1909, 1984
Bse21I	CCTNAGG	1 cut(s)	1079
Bse3DI	GCAATG	2 cut(s)	1938, 2020
BseBI	CCWGG	2 cut(s)	1093, 1968
BseDI	CCNNGG	6 cut(s)	81, 1031, 1092, 1562, 1891, 1967
BseGI	GGATG	7 cut(s)	524, 559, 1409, 1551, 1834, 2038, 2106
BseLI	CCNNNNNNNGG	1 cut(s)	1656
BseMI	GCAATG	2 cut(s)	1938, 2020
BseMII	CTCAG	6 cut(s)	380, 389, 690, 1016, 1070, 1589
BseNI	ACTGG	7 cut(s)	192, 598, 787, 1458, 1696, 1909, 1984
BseRI	GAGGAG	2 cut(s)	1120, 1314
BseSI	GKGCMC	1 cut(s)	1355
BseX3I	CGGCCG	1 cut(s)	102
BseXI	GCAGC	7 cut(s)	204, 232, 951, 1773, 1955, 2019, 2022
BseYI	CCCAGC	1 cut(s)	1883
BsgI	GTGCAG	2 cut(s)	858, 1230
Bsh1236I	CGCG	3 cut(s)	107, 287, 294
Bsh1285I	CGRYCG	4 cut(s)	58, 70, 105, 1137
BshFI	GGCC	6 cut(s)	9, 104, 561, 645, 1567, 1677
BshNI	GGYRCC	4 cut(s)	50, 1350, 1659, 2078
BsiEI	CGRYCG	4 cut(s)	58, 70, 105, 1137
BsiHKAI	GWGCWC	2 cut(s)	125, 605
BsiHKCI	CYCGRG	1 cut(s)	124
BsiSI	CCGG	6 cut(s)	6, 241, 646, 1359, 1555, 1650
BslFI	GGGAC	5 cut(s)	215, 1060, 1130, 1318, 1497
BslI	CCNNNNNNNGG	1 cut(s)	1656
BsmAI	GTCTC	3 cut(s)	695, 1061, 1766
BsmFI	GGGAC	5 cut(s)	215, 1060, 1130, 1318, 1497
BsmI	GAATGC	3 cut(s)	334, 408, 1695
BsnI	GGCC	6 cut(s)	9, 104, 561, 645, 1567, 1677
Bso31I	GGTCTC	1 cut(s)	1061
BsoBI	CYCGRG	1 cut(s)	124
Bsp119I	TTCGAA	2 cut(s)	335, 2090
Bsp1286I	GDGCHC	3 cut(s)	125, 605, 1355
BspACI	CCGC	7 cut(s)	105, 172, 345, 392, 861, 1133, 1680
BspANI	GGCC	6 cut(s)	9, 104, 561, 645, 1567, 1677
BspCNI	CTCAG	6 cut(s)	379, 388, 691, 1017, 1071, 1588
BspFNI	CGCG	3 cut(s)	107, 287, 294
BspLI	GGNNCC	8 cut(s)	52, 1352, 1661, 1678, 1889, 1909, 2059, 2080
BspMAI	CTGCAG	1 cut(s)	222
BspMI	ACCTGC	3 cut(s)	265, 1462, 1846
BspOI	GCTAGC	1 cut(s)	410
BspPI	GGATC	9 cut(s)	753, 850, 871, 1369, 1576, 1604, 2047, 2052, 2065
BspQI	GCTCTTC	2 cut(s)	162, 1651
BspT104I	TTCGAA	2 cut(s)	335, 2090
BspT107I	GGYRCC	4 cut(s)	50, 1350, 1659, 2078
BspTNI	GGTCTC	1 cut(s)	1061
BsrBI	CCGCTC	1 cut(s)	172
BsrDI	GCAATG	2 cut(s)	1938, 2020
BsrFI	RCCGGY	2 cut(s)	5, 645
BsrI	ACTGG	7 cut(s)	192, 598, 787, 1458, 1696, 1909, 1984
BssAI	RCCGGY	2 cut(s)	5, 645
BssECI	CCNNGG	6 cut(s)	81, 1031, 1092, 1562, 1891, 1967
BssNI	GRCGYC	2 cut(s)	51, 140
BssT1I	CCWWGG	2 cut(s)	1031, 1562
Bst2UI	CCWGG	2 cut(s)	1093, 1968
Bst4CI	ACNGT	5 cut(s)	514, 850, 1276, 1346, 1990
Bst6I	CTCTTC	2 cut(s)	162, 1651
BstACI	GRCGYC	2 cut(s)	51, 140
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstBI	TTCGAA	2 cut(s)	335, 2090
BstC8I	GCNNGC	4 cut(s)	363, 390, 408, 1675
BstDEI	CTNAG	6 cut(s)	366, 375, 699, 1025, 1079, 1575
BstDSI	CCRYGG	1 cut(s)	1891
BstF5I	GGATG	7 cut(s)	524, 559, 1409, 1551, 1834, 2038, 2106
BstFNI	CGCG	3 cut(s)	107, 287, 294
BstH2I	RGCGCY	2 cut(s)	54, 266
BstHHI	GCGC	5 cut(s)	53, 265, 287, 296, 698
BstMAI	GTCTC	3 cut(s)	695, 1061, 1766
BstMCI	CGRYCG	4 cut(s)	58, 70, 105, 1137
BstNI	CCWGG	2 cut(s)	1093, 1968
BstSCI	CCNGG	4 cut(s)	240, 1091, 1357, 1966
BstSFI	CTRYAG	2 cut(s)	218, 609
BstSLI	GKGCMC	1 cut(s)	1355
BstUI	CGCG	3 cut(s)	107, 287, 294
BstV1I	GCAGC	7 cut(s)	204, 232, 951, 1773, 1955, 2019, 2022
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	5 cut(s)	208, 745, 1581, 2039, 2057
BstXI	CCANNNNNNTGG	1 cut(s)	1954
BstYI	RGATCY	5 cut(s)	208, 745, 1581, 2039, 2057
BstZI	CGGCCG	1 cut(s)	102
Bsu36I	CCTNAGG	1 cut(s)	1079
BsuI	GTATCC	1 cut(s)	177
BsuRI	GGCC	6 cut(s)	9, 104, 561, 645, 1567, 1677
BtgI	CCRYGG	1 cut(s)	1891
BtrI	CACGTC	1 cut(s)	800
BtsCI	GGATG	7 cut(s)	524, 559, 1409, 1551, 1834, 2038, 2106
BtsI	GCAGTG	3 cut(s)	257, 1167, 1961
BtsIMutI	CAGTG	8 cut(s)	257, 605, 855, 1167, 1675, 1820, 1902, 1961
BveI	ACCTGC	3 cut(s)	265, 1462, 1846
Cac8I	GCNNGC	4 cut(s)	363, 390, 408, 1675
CfoI	GCGC	5 cut(s)	53, 265, 287, 296, 698
Cfr10I	RCCGGY	2 cut(s)	5, 645
Cfr13I	GGNCC	2 cut(s)	238, 1676
CseI	GACGC	1 cut(s)	1769
Csp6I	GTAC	4 cut(s)	478, 1378, 1660, 2079
CspCI	CAANNNNNGTGG	2 cut(s)	1482, 1517
CviAII	CATG	3 cut(s)	1315, 1843, 1948
CviQI	GTAC	4 cut(s)	478, 1378, 1660, 2079
DdeI	CTNAG	6 cut(s)	366, 375, 699, 1025, 1079, 1575
DinI	GGCGCC	1 cut(s)	52
DriI	GACNNNNNGTC	1 cut(s)	1779
EaeI	YGGCCR	4 cut(s)	7, 102, 559, 643
EagI	CGGCCG	1 cut(s)	102
Eam1104I	CTCTTC	2 cut(s)	162, 1651
Eam1105I	GACNNNNNGTC	1 cut(s)	1779
EarI	CTCTTC	2 cut(s)	162, 1651
Ecl136II	GAGCTC	1 cut(s)	123
EclXI	CGGCCG	1 cut(s)	102
Eco130I	CCWWGG	2 cut(s)	1031, 1562
Eco24I	GRGCYC	1 cut(s)	125
Eco31I	GGTCTC	1 cut(s)	1061
Eco32I	GATATC	1 cut(s)	1974
Eco47I	GGWCC	1 cut(s)	238
Eco47III	AGCGCT	1 cut(s)	264
Eco52I	CGGCCG	1 cut(s)	102
Eco53kI	GAGCTC	1 cut(s)	123
Eco57I	CTGAAG	1 cut(s)	341
Eco81I	CCTNAGG	1 cut(s)	1079
Eco88I	CYCGRG	1 cut(s)	124
EcoICRI	GAGCTC	1 cut(s)	123
EcoRI	GAATTC	1 cut(s)	86
EcoRII	CCWGG	2 cut(s)	1091, 1966
EcoRV	GATATC	1 cut(s)	1974
EcoT14I	CCWWGG	2 cut(s)	1031, 1562
EcoT22I	ATGCAT	1 cut(s)	1177
EcoT38I	GRGCYC	1 cut(s)	125
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
ErhI	CCWWGG	2 cut(s)	1031, 1562
FaeI	CATG	3 cut(s)	1318, 1846, 1951
FalI	AAGNNNNNCTT	4 cut(s)	1555, 1587, 1647, 1679
FaqI	GGGAC	5 cut(s)	215, 1060, 1130, 1318, 1497
FatI	CATG	3 cut(s)	1314, 1842, 1947
FauI	CCCGC	1 cut(s)	1687
FbaI	TGATCA	1 cut(s)	750
Fnu4HI	GCNGC	9 cut(s)	105, 218, 221, 345, 965, 1787, 1944, 2008, 2011
FokI	GGATG	7 cut(s)	511, 566, 1396, 1538, 1841, 2045, 2113
FriOI	GRGCYC	1 cut(s)	125
Fsp4HI	GCNGC	9 cut(s)	105, 218, 221, 345, 965, 1787, 1944, 2008, 2011
FspBI	CTAG	3 cut(s)	407, 1767, 2043
GlaI	GCGC	5 cut(s)	52, 264, 286, 295, 697
GluI	GCNGC	9 cut(s)	105, 218, 221, 345, 965, 1787, 1944, 2008, 2011
GsaI	CCCAGC	1 cut(s)	1887
GsuI	CTGGAG	2 cut(s)	1274, 1387
HaeII	RGCGCY	2 cut(s)	54, 266
HaeIII	GGCC	6 cut(s)	9, 104, 561, 645, 1567, 1677
HapII	CCGG	6 cut(s)	6, 241, 646, 1359, 1555, 1650
HgaI	GACGC	1 cut(s)	1769
HhaI	GCGC	5 cut(s)	53, 265, 287, 296, 698
Hin1I	GRCGYC	2 cut(s)	51, 140
Hin1II	CATG	3 cut(s)	1318, 1846, 1951
Hin6I	GCGC	5 cut(s)	51, 263, 285, 294, 696
HinP1I	GCGC	5 cut(s)	51, 263, 285, 294, 696
HincII	GTYRAC	5 cut(s)	177, 372, 780, 948, 1479
HindII	GTYRAC	5 cut(s)	177, 372, 780, 948, 1479
HindIII	AAGCTT	1 cut(s)	710
HpaI	GTTAAC	1 cut(s)	780
HpaII	CCGG	6 cut(s)	6, 241, 646, 1359, 1555, 1650
HphI	GGTGA	3 cut(s)	571, 812, 1809
Hpy166II	GTNNAC	6 cut(s)	177, 372, 780, 948, 1479, 1621
Hpy188I	TCNGA	5 cut(s)	67, 529, 750, 1195, 1614
Hpy188III	TCNNGA	6 cut(s)	118, 754, 929, 972, 1253, 1541
Hpy8I	GTNNAC	6 cut(s)	177, 372, 780, 948, 1479, 1621
Hpy99I	CGWCG	8 cut(s)	41, 50, 103, 142, 145, 148, 1785, 1919
HpyAV	CCTTC	8 cut(s)	188, 344, 736, 833, 991, 1600, 1753, 2092
HpyCH4III	ACNGT	5 cut(s)	514, 850, 1276, 1346, 1990
HpyCH4IV	ACGT	4 cut(s)	140, 535, 799, 1917
HpyF3I	CTNAG	6 cut(s)	366, 375, 699, 1025, 1079, 1575
HpySE526I	ACGT	4 cut(s)	140, 535, 799, 1917
Hsp92I	GRCGYC	2 cut(s)	51, 140
Hsp92II	CATG	3 cut(s)	1318, 1846, 1951
HspAI	GCGC	5 cut(s)	51, 263, 285, 294, 696
KasI	GGCGCC	1 cut(s)	50
KpnI	GGTACC	2 cut(s)	1663, 2082
Ksp22I	TGATCA	1 cut(s)	750
KspAI	GTTAAC	1 cut(s)	780
LguI	GCTCTTC	2 cut(s)	162, 1651
LmnI	GCTCC	4 cut(s)	120, 134, 910, 1887
Lsp1109I	GCAGC	7 cut(s)	204, 232, 951, 1773, 1955, 2019, 2022
LweI	GCATC	3 cut(s)	331, 1184, 1307
MaeI	CTAG	3 cut(s)	407, 1767, 2043
MaeII	ACGT	4 cut(s)	140, 535, 799, 1917
MaeIII	GTNAC	2 cut(s)	795, 1257
MbiI	CCGCTC	1 cut(s)	172
MboII	GAAGA	8 cut(s)	32, 179, 334, 1230, 1434, 1529, 1638, 2081
MflI	RGATCY	5 cut(s)	208, 745, 1581, 2039, 2057
MhlI	GDGCHC	3 cut(s)	125, 605, 1355
MlsI	TGGCCA	1 cut(s)	561
MluNI	TGGCCA	1 cut(s)	561
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	6 cut(s)	89, 367, 803, 1036, 1265, 1768
MmeI	TCCRAC	6 cut(s)	379, 427, 835, 1303, 1500, 1734
Mox20I	TGGCCA	1 cut(s)	561
Mph1103I	ATGCAT	1 cut(s)	1177
MroXI	GAANNNNTTC	1 cut(s)	24
MscI	TGGCCA	1 cut(s)	561
MseI	TTAA	4 cut(s)	584, 779, 1448, 1497
MslI	CAYNNNNRTG	2 cut(s)	825, 1952
Msp20I	TGGCCA	1 cut(s)	561
MspA1I	CMGCKG	2 cut(s)	392, 1133
MspI	CCGG	6 cut(s)	6, 241, 646, 1359, 1555, 1650
MspR9I	CCNGG	4 cut(s)	242, 1093, 1359, 1968
Mva1269I	GAATGC	3 cut(s)	334, 408, 1695
MvaI	CCWGG	2 cut(s)	1093, 1968
MvnI	CGCG	3 cut(s)	107, 287, 294
NarI	GGCGCC	1 cut(s)	51
NciI	CCSGG	2 cut(s)	242, 1359
NheI	GCTAGC	1 cut(s)	406
NlaIII	CATG	3 cut(s)	1318, 1846, 1951
NlaIV	GGNNCC	8 cut(s)	52, 1352, 1661, 1678, 1889, 1909, 2059, 2080
NmeAIII	GCCGAG	2 cut(s)	106, 799
NmuCI	GTSAC	2 cut(s)	795, 1257
NsiI	ATGCAT	1 cut(s)	1177
NspV	TTCGAA	2 cut(s)	335, 2090
OliI	CACNNNNGTG	1 cut(s)	825
PciSI	GCTCTTC	2 cut(s)	162, 1651
PcsI	WCGNNNNNNNCGW	4 cut(s)	36, 42, 51, 104
PctI	GAATGC	3 cut(s)	334, 408, 1695
PdmI	GAANNNNTTC	1 cut(s)	24
PfeI	GAWTC	8 cut(s)	505, 625, 786, 985, 1418, 1723, 1732, 1745
PflFI	GACNNNGTC	1 cut(s)	142
PfoI	TCCNGGA	1 cut(s)	1357
PkrI	GCNGC	9 cut(s)	106, 219, 222, 346, 966, 1788, 1945, 2009, 2012
Ple19I	CGATCG	2 cut(s)	58, 70
PleI	GAGTC	6 cut(s)	89, 367, 802, 1035, 1264, 1768
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	6 cut(s)	89, 367, 802, 1035, 1264, 1768
Psp124BI	GAGCTC	1 cut(s)	125
Psp6I	CCWGG	2 cut(s)	1091, 1966
PspFI	CCCAGC	1 cut(s)	1883
PspGI	CCWGG	2 cut(s)	1091, 1966
PspN4I	GGNNCC	8 cut(s)	52, 1352, 1661, 1678, 1889, 1909, 2059, 2080
PspPI	GGNCC	2 cut(s)	238, 1676
PsrI	GAACNNNNNNTAC	2 cut(s)	2071, 2103
PstI	CTGCAG	1 cut(s)	222
PsuI	RGATCY	5 cut(s)	208, 745, 1581, 2039, 2057
PsyI	GACNNNGTC	1 cut(s)	142
PvuI	CGATCG	2 cut(s)	58, 70
RsaI	GTAC	4 cut(s)	479, 1379, 1661, 2080
RsaNI	GTAC	4 cut(s)	478, 1378, 1660, 2079
RseI	CAYNNNNRTG	2 cut(s)	825, 1952
SacI	GAGCTC	1 cut(s)	125
SapI	GCTCTTC	2 cut(s)	162, 1651
SaqAI	TTAA	4 cut(s)	584, 779, 1448, 1497
SatI	GCNGC	9 cut(s)	105, 218, 221, 345, 965, 1787, 1944, 2008, 2011
Sau96I	GGNCC	2 cut(s)	238, 1676
ScaI	AGTACT	1 cut(s)	1379
SchI	GAGTC	6 cut(s)	89, 367, 803, 1036, 1265, 1768
ScrFI	CCNGG	4 cut(s)	242, 1093, 1359, 1968
SduI	GDGCHC	3 cut(s)	125, 605, 1355
SfaNI	GCATC	3 cut(s)	331, 1184, 1307
SfcI	CTRYAG	2 cut(s)	218, 609
SfoI	GGCGCC	1 cut(s)	52
SfuI	TTCGAA	2 cut(s)	335, 2090
SinI	GGWCC	1 cut(s)	238
SmiMI	CAYNNNNRTG	2 cut(s)	825, 1952
SmlI	CTYRAG	1 cut(s)	927
SmoI	CTYRAG	1 cut(s)	927
SsiI	CCGC	7 cut(s)	105, 172, 345, 392, 861, 1133, 1680
SspDI	GGCGCC	1 cut(s)	50
SspMI	CTAG	3 cut(s)	407, 1767, 2043
SstI	GAGCTC	1 cut(s)	125
StyD4I	CCNGG	4 cut(s)	240, 1091, 1357, 1966
StyI	CCWWGG	2 cut(s)	1031, 1562
TaaI	ACNGT	5 cut(s)	514, 850, 1276, 1346, 1990
TaiI	ACGT	4 cut(s)	143, 538, 802, 1920
TaqI	TCGA	6 cut(s)	45, 90, 335, 973, 1137, 2090
TaqII	GACCGA	1 cut(s)	60
TatI	WGTACW	1 cut(s)	1377
TauI	GCSGC	2 cut(s)	107, 347
TfiI	GAWTC	8 cut(s)	505, 625, 786, 985, 1418, 1723, 1732, 1745
Tru1I	TTAA	4 cut(s)	584, 779, 1448, 1497
Tru9I	TTAA	4 cut(s)	584, 779, 1448, 1497
TscAI	CASTG	8 cut(s)	257, 605, 855, 1167, 1675, 1827, 1909, 1968
TseFI	GTSAC	2 cut(s)	795, 1257
TseI	GCWGC	7 cut(s)	217, 220, 964, 1786, 1943, 2007, 2010
Tsp45I	GTSAC	2 cut(s)	795, 1257
TspDTI	ATGAA	9 cut(s)	341, 531, 643, 755, 996, 1010, 1230, 1736, 2117
TspGWI	ACGGA	2 cut(s)	219, 948
TspRI	CASTG	8 cut(s)	257, 605, 855, 1167, 1675, 1827, 1909, 1968
Tth111I	GACNNNGTC	1 cut(s)	142
VpaK11BI	GGWCC	1 cut(s)	238
XapI	RAATTY	3 cut(s)	86, 1003, 1305
XmnI	GAANNNNTTC	1 cut(s)	24
XspI	CTAG	3 cut(s)	407, 1767, 2043
ZraI	GACGTC	1 cut(s)	141
ZrmI	AGTACT	1 cut(s)	1379
Zsp2I	ATGCAT	1 cut(s)	1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.