RchiOBHm_Chr1g0381921

OPT oligopeptide transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004564
Physical Location & Seq
Forward (+)
67025066 .. 67026630
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60499

Sequence Viewer

Length: 1407 bp
ATGAAAGGCCTTGGAATCGGTTCTTTTGGATTGAGTGGTCTGCTATTGCAGCATACCTTAGTACTCCTTTGGCTAGCCCGTGGTTCGCCACAGCCAACATTGCTGTTGGATTTTTCTTTCTCATGCATGTATGTAATGACACCCATCACTTACTGGTCTAATATCTACAGCGCCAAGACATTCCCAATATTTTCTAGCAATTTGTTCATGGCTAATGGTTCAAGATACGAGGTCCTTGATATCATTGATTCCAGGTTTCAACTTGATCATGGCGAATATGCAAAGAAGGGGCCTCTGCATATGAGCAGCTTTTTTGCTTTTACTTACGGTCTTGGATTTGCCACTCTTTCTGCTACAGTTATGCATGTTCTACTTTTTAATGCAAGGAAACATTATTTGATTTTCTATGCAGGGCTTATGAAGAGTTACAAATCAGTGCCAACATTGTGGTTTATCCTCATCCTTGTGATCAATATTGCTCTTATCCTGTTTGCTTGTGTGTATTACAAGGAGTCACTTCAACTGCCTTGGTGGGGTGCATTGCTTGCTTGTTTCATTGCCGTTGCGTTCACGTACCCAATTGGTGTCATCCAAGCCACCACAAACCAGCAACCAGGTTTGAATATTATCACAGAATATGTGATAGGATACCTTTATCCTGAGCGCCCTGTAGCTAACATGTGCTTCAAAGTGTATGGTTACATAAGTATGATGCAAGGTCTGCATTTTGTGTCGGATTTTAAGCTCGGTCACTACATGAAGATTCCACCAAGAGCAATGTTCACAGCACAGATGGTAGGAACTGTTGTAGCAGTGGTTGTATACCAGTGTACAGCATGGTGGCTGATGGCAGACATTCCCAACCTCTGTGATACCTCCCTGCTGCCACCGGACAGCCCTTGGACTTGTCCAACGGACCGCGTGTTCTTTGATGCTTCTGTTATCTGGGGTCTTGTTGGACCCCGCAGAATTTTTGGAAACCTCCGTGAATACAGTAACATCACCTGGTTTTTCCTTGGTGGCGCCATTGCCCCGGTGCTTGTTTGGCTTGCACACAGGATTTTTCCCAAACAAAAATGGATTCTTGCTGTTAACATGCCTGTCTTACTAGGTGCCACGGCAATGATGCCCCCTGCCACTGCTTTGAATTTTCAAAGTTGGATATTTGTGGCATTTCTTTCAGGTTTTGTTGTCTTCAGGTACCGGCAAGATTTGTGGAAGAAGTATAACTATGTCGCCTCTGGTGCTCTCGAGGCCGGAACTGCCTTTGTTACGATATTGGTGTTCATTACCTTGCAGTCCAAGAATATTGAACTCGACTGGTGGGGGAACAAAGGAGAGCTCTGCCCCCTGGCTACATGCCCTACTGCGAAAGGGGTTGTCATTGCTGGCTGCCCGGTCCTCTAG

Protein Analysis

468

Amino Acids

52.61

Weight (kDa)

9.02

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 40 - 430 2.7e-85 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1200
AccB1I GGYRCC 3 cut(s) 1022, 1112, 1200
AccI GTMKAC 1 cut(s) 822
AccII CGCG 1 cut(s) 921
AciI CCGC 2 cut(s) 919, 964
AcsI RAATTY 2 cut(s) 969, 1147
AcuI CTGAAG 1 cut(s) 1180
AcyI GRCGYC 1 cut(s) 1023
AfaI GTAC 4 cut(s) 63, 575, 832, 1202
AfiI CCNNNNNNNGG 1 cut(s) 533
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 8 cut(s) 222, 260, 521, 622, 688, 1147, 1154, 1313
AjnI CCWGG 4 cut(s) 251, 613, 1004, 1350
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
AluBI AGCT 4 cut(s) 309, 674, 745, 1342
AluI AGCT 4 cut(s) 309, 674, 745, 1342
Alw21I GWGCWC 2 cut(s) 1249, 1344
Ama87I CYCGRG 1 cut(s) 1250
AoxI GGCC 3 cut(s) 7, 290, 1254
ApeKI GCWGC 4 cut(s) 49, 306, 883, 1392
ApoI RAATTY 2 cut(s) 969, 1147
Asp700I GAANNNNTTC 1 cut(s) 19
Asp718I GGTACC 1 cut(s) 1200
AspLEI GCGC 3 cut(s) 173, 666, 1025
AspS9I GGNCC 5 cut(s) 232, 290, 916, 959, 1399
AsuC2I CCSGG 2 cut(s) 1034, 1397
AsuHPI GGTGA 1 cut(s) 994
AsuNHI GCTAGC 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 4 cut(s) 232, 916, 959, 1399
BanI GGYRCC 3 cut(s) 1022, 1112, 1200
BanII GRGCYC 1 cut(s) 1344
BbsI GAAGAC 1 cut(s) 1186
Bbv12I GWGCWC 2 cut(s) 1249, 1344
BbvI GCAGC 4 cut(s) 61, 318, 870, 1379
BccI CCATC 3 cut(s) 152, 787, 841
BceAI ACGGC 2 cut(s) 545, 1134
BciT130I CCWGG 4 cut(s) 253, 615, 1006, 1352
BciVI GTATCC 1 cut(s) 641
BclI TGATCA 2 cut(s) 265, 468
BcnI CCSGG 2 cut(s) 1034, 1397
BfaI CTAG 4 cut(s) 74, 195, 1109, 1405
BfmI CTRYAG 3 cut(s) 166, 354, 669
BfoI RGCGCY 3 cut(s) 174, 667, 1026
BfuI GTATCC 1 cut(s) 641
BisI GCNGC 4 cut(s) 50, 307, 884, 1393
BlsI GCNGC 4 cut(s) 51, 308, 885, 1394
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 6 cut(s) 253, 615, 1006, 1034, 1352, 1397
Bme18I GGWCC 4 cut(s) 232, 916, 959, 1399
BmeT110I CYCGRG 1 cut(s) 1250
BmgT120I GGNCC 5 cut(s) 232, 290, 916, 959, 1399
BmiI GGNNCC 5 cut(s) 291, 961, 1024, 1114, 1202
BmrFI CCNGG 6 cut(s) 253, 615, 1006, 1034, 1352, 1397
BmsI GCATC 3 cut(s) 702, 922, 1116
BmtI GCTAGC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 1186
Bpu10I CCTNAGC 1 cut(s) 660
BpuMI CCSGG 2 cut(s) 1034, 1397
BsaAI YACGTR 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 1023
BsaJI CCNNGG 8 cut(s) 10, 79, 527, 899, 1015, 1032, 1116, 1350
BsaWI WCCGGW 1 cut(s) 889
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse118I RCCGGY 1 cut(s) 1203
Bse1I ACTGG 3 cut(s) 158, 826, 1325
Bse3DI GCAATG 7 cut(s) 98, 539, 555, 783, 1026, 1128, 1383
BseBI CCWGG 4 cut(s) 253, 615, 1006, 1352
BseDI CCNNGG 8 cut(s) 10, 79, 527, 899, 1015, 1032, 1116, 1350
BseGI GGATG 2 cut(s) 459, 588
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMI GCAATG 7 cut(s) 98, 539, 555, 783, 1026, 1128, 1383
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 3 cut(s) 158, 826, 1325
BseXI GCAGC 4 cut(s) 61, 318, 870, 1379
Bsh1236I CGCG 1 cut(s) 921
BshFI GGCC 3 cut(s) 9, 292, 1256
BshNI GGYRCC 3 cut(s) 1022, 1112, 1200
BsiHKAI GWGCWC 2 cut(s) 1249, 1344
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 5 cut(s) 890, 1034, 1204, 1257, 1397
BslI CCNNNNNNNGG 1 cut(s) 533
BsnI GGCC 3 cut(s) 9, 292, 1256
BsoBI CYCGRG 1 cut(s) 1250
Bsp1286I GDGCHC 2 cut(s) 1249, 1344
Bsp1407I TGTACA 1 cut(s) 830
Bsp143I GATC 2 cut(s) 265, 468
BspACI CCGC 2 cut(s) 919, 964
BspANI GGCC 3 cut(s) 9, 292, 1256
BspCNI CTCAG 1 cut(s) 652
BspFNI CGCG 1 cut(s) 921
BspLI GGNNCC 5 cut(s) 291, 961, 1024, 1114, 1202
BspOI GCTAGC 1 cut(s) 77
BspT107I GGYRCC 3 cut(s) 1022, 1112, 1200
BsrDI GCAATG 7 cut(s) 98, 539, 555, 783, 1026, 1128, 1383
BsrFI RCCGGY 1 cut(s) 1203
BsrGI TGTACA 1 cut(s) 830
BsrI ACTGG 3 cut(s) 158, 826, 1325
BssAI RCCGGY 1 cut(s) 1203
BssECI CCNNGG 8 cut(s) 10, 79, 527, 899, 1015, 1032, 1116, 1350
BssMI GATC 2 cut(s) 265, 468
BssNAI GTATAC 1 cut(s) 823
BssNI GRCGYC 1 cut(s) 1023
BssT1I CCWWGG 4 cut(s) 10, 527, 899, 1015
Bst1107I GTATAC 1 cut(s) 823
Bst2UI CCWGG 4 cut(s) 253, 615, 1006, 1352
Bst4CI ACNGT 4 cut(s) 329, 358, 805, 995
Bst6I CTCTTC 1 cut(s) 416
BstACI GRCGYC 1 cut(s) 1023
BstAPI GCANNNNNTGC 2 cut(s) 545, 721
BstAUI TGTACA 1 cut(s) 830
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 4 cut(s) 75, 546, 1050, 1390
BstDEI CTNAG 2 cut(s) 58, 660
BstDSI CCRYGG 2 cut(s) 79, 1116
BstF5I GGATG 2 cut(s) 459, 588
BstFNI CGCG 1 cut(s) 921
BstH2I RGCGCY 3 cut(s) 174, 667, 1026
BstHHI GCGC 3 cut(s) 173, 666, 1025
BstKTI GATC 2 cut(s) 268, 471
BstMBI GATC 2 cut(s) 265, 468
BstMWI GCNNNNNNNGC 8 cut(s) 49, 100, 545, 721, 1045, 1244, 1253, 1262
BstNI CCWGG 4 cut(s) 253, 615, 1006, 1352
BstNSI RCATGY 5 cut(s) 130, 368, 682, 1099, 1362
BstSCI CCNGG 6 cut(s) 251, 613, 1004, 1032, 1350, 1395
BstSFI CTRYAG 3 cut(s) 166, 354, 669
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 4 cut(s) 61, 318, 870, 1379
BstV2I GAAGAC 1 cut(s) 1186
BstXI CCANNNNNNTGG 1 cut(s) 447
BstZ17I GTATAC 1 cut(s) 823
BsuI GTATCC 1 cut(s) 641
BsuRI GGCC 3 cut(s) 9, 292, 1256
BtgI CCRYGG 2 cut(s) 79, 1116
BtsCI GGATG 2 cut(s) 459, 588
BtsI GCAGTG 2 cut(s) 819, 1137
BtsIMutI CAGTG 4 cut(s) 441, 819, 833, 1137
Cac8I GCNNGC 4 cut(s) 75, 546, 1050, 1390
CfoI GCGC 3 cut(s) 173, 666, 1025
Cfr10I RCCGGY 1 cut(s) 1203
Cfr13I GGNCC 5 cut(s) 232, 290, 916, 959, 1399
CpoI CGGWCCG 1 cut(s) 916
CsiI ACCWGGT 2 cut(s) 613, 1004
Csp6I GTAC 4 cut(s) 62, 574, 831, 1201
CspCI CAANNNNNGTGG 2 cut(s) 1196, 1231
CspI CGGWCCG 1 cut(s) 916
CviQI GTAC 4 cut(s) 62, 574, 831, 1201
DdeI CTNAG 2 cut(s) 58, 660
DinI GGCGCC 1 cut(s) 1024
DpnI GATC 2 cut(s) 267, 470
DpnII GATC 2 cut(s) 265, 468
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 1342
Eco130I CCWWGG 4 cut(s) 10, 527, 899, 1015
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 1344
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 4 cut(s) 232, 916, 959, 1399
Eco53kI GAGCTC 1 cut(s) 1342
Eco57I CTGAAG 1 cut(s) 1180
Eco88I CYCGRG 1 cut(s) 1250
EcoICRI GAGCTC 1 cut(s) 1342
EcoO109I RGGNCCY 2 cut(s) 232, 290
EcoRII CCWGG 4 cut(s) 251, 613, 1004, 1350
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 4 cut(s) 10, 527, 899, 1015
EcoT22I ATGCAT 2 cut(s) 128, 366
EcoT38I GRGCYC 1 cut(s) 1344
EgeI GGCGCC 1 cut(s) 1024
EheI GGCGCC 1 cut(s) 1024
ErhI CCWWGG 4 cut(s) 10, 527, 899, 1015
FauI CCCGC 1 cut(s) 971
FauNDI CATATG 1 cut(s) 300
FbaI TGATCA 2 cut(s) 265, 468
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 4 cut(s) 50, 307, 884, 1393
FokI GGATG 2 cut(s) 446, 575
FriOI GRGCYC 1 cut(s) 1344
Fsp4HI GCNGC 4 cut(s) 50, 307, 884, 1393
FspBI CTAG 4 cut(s) 74, 195, 1109, 1405
GlaI GCGC 3 cut(s) 172, 665, 1024
GluI GCNGC 4 cut(s) 50, 307, 884, 1393
HaeII RGCGCY 3 cut(s) 174, 667, 1026
HaeIII GGCC 3 cut(s) 9, 292, 1256
HapII CCGG 5 cut(s) 890, 1034, 1204, 1257, 1397
HhaI GCGC 3 cut(s) 173, 666, 1025
Hin1I GRCGYC 1 cut(s) 1023
Hin6I GCGC 3 cut(s) 171, 664, 1023
HinP1I GCGC 3 cut(s) 171, 664, 1023
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HinfI GANTC 5 cut(s) 15, 248, 512, 763, 1081
HpaI GTTAAC 1 cut(s) 1093
HpaII CCGG 5 cut(s) 890, 1034, 1204, 1257, 1397
HphI GGTGA 1 cut(s) 994
Hpy166II GTNNAC 5 cut(s) 570, 783, 823, 831, 1093
Hpy188I TCNGA 1 cut(s) 736
Hpy188III TCNNGA 3 cut(s) 222, 659, 1250
Hpy8I GTNNAC 5 cut(s) 570, 783, 823, 831, 1093
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 4 cut(s) 329, 358, 805, 995
HpyCH4IV ACGT 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 100, 545, 721, 1045, 1244, 1253, 1262
HpyF3I CTNAG 2 cut(s) 58, 660
HpySE526I ACGT 1 cut(s) 572
Hsp92I GRCGYC 1 cut(s) 1023
HspAI GCGC 3 cut(s) 171, 664, 1023
KasI GGCGCC 1 cut(s) 1022
KpnI GGTACC 1 cut(s) 1204
Ksp22I TGATCA 2 cut(s) 265, 468
KspAI GTTAAC 1 cut(s) 1093
Kzo9I GATC 2 cut(s) 265, 468
Lsp1109I GCAGC 4 cut(s) 61, 318, 870, 1379
LweI GCATC 3 cut(s) 702, 922, 1116
MabI ACCWGGT 2 cut(s) 613, 1004
MaeI CTAG 4 cut(s) 74, 195, 1109, 1405
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 6 cut(s) 425, 513, 698, 749, 995, 1270
MalI GATC 2 cut(s) 267, 470
MboI GATC 2 cut(s) 265, 468
MboII GAAGA 4 cut(s) 433, 772, 1186, 1231
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 2 cut(s) 1249, 1344
MluCI AATT 4 cut(s) 199, 579, 969, 1147
Mly113I GGCGCC 1 cut(s) 1023
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 5 cut(s) 87, 714, 935, 937, 1139
MnlI CCTC 8 cut(s) 223, 303, 467, 875, 886, 992, 1246, 1249
Mph1103I ATGCAT 2 cut(s) 128, 366
MroXI GAANNNNTTC 1 cut(s) 19
MseI TTAA 3 cut(s) 378, 741, 1092
MslI CAYNNNNRTG 3 cut(s) 464, 707, 1121
MspI CCGG 5 cut(s) 890, 1034, 1204, 1257, 1397
MspR9I CCNGG 6 cut(s) 253, 615, 1006, 1034, 1352, 1397
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 4 cut(s) 253, 615, 1006, 1352
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 8 cut(s) 49, 100, 545, 721, 1045, 1244, 1253, 1262
NarI GGCGCC 1 cut(s) 1023
NciI CCSGG 2 cut(s) 1034, 1397
NdeI CATATG 1 cut(s) 300
NdeII GATC 2 cut(s) 265, 468
NheI GCTAGC 1 cut(s) 73
NlaIV GGNNCC 5 cut(s) 291, 961, 1024, 1114, 1202
NmuCI GTSAC 2 cut(s) 513, 749
NsiI ATGCAT 2 cut(s) 128, 366
NspI RCATGY 5 cut(s) 130, 368, 682, 1099, 1362
PaeR7I CTCGAG 1 cut(s) 1250
PceI AGGCCT 1 cut(s) 9
PciI ACATGT 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 4 cut(s) 15, 248, 763, 1081
PkrI GCNGC 4 cut(s) 51, 308, 885, 1394
PleI GAGTC 1 cut(s) 520
PluTI GGCGCC 1 cut(s) 1026
PpsI GAGTC 1 cut(s) 520
Ppu21I YACGTR 1 cut(s) 573
PpuMI RGGWCCY 1 cut(s) 232
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 1344
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 4 cut(s) 251, 613, 1004, 1350
PspGI CCWGG 4 cut(s) 251, 613, 1004, 1350
PspN4I GGNNCC 5 cut(s) 291, 961, 1024, 1114, 1202
PspPI GGNCC 5 cut(s) 232, 290, 916, 959, 1399
PspPPI RGGWCCY 1 cut(s) 232
RsaI GTAC 4 cut(s) 63, 575, 832, 1202
RsaNI GTAC 4 cut(s) 62, 574, 831, 1201
RseI CAYNNNNRTG 3 cut(s) 464, 707, 1121
Rsr2I CGGWCCG 1 cut(s) 916
RsrII CGGWCCG 1 cut(s) 916
SacI GAGCTC 1 cut(s) 1344
SaqAI TTAA 3 cut(s) 378, 741, 1092
SatI GCNGC 4 cut(s) 50, 307, 884, 1393
Sau3AI GATC 2 cut(s) 265, 468
Sau96I GGNCC 5 cut(s) 232, 290, 916, 959, 1399
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 6 cut(s) 253, 615, 1006, 1034, 1352, 1397
SduI GDGCHC 2 cut(s) 1249, 1344
SexAI ACCWGGT 2 cut(s) 613, 1004
SfaNI GCATC 3 cut(s) 702, 922, 1116
SfcI CTRYAG 3 cut(s) 166, 354, 669
SfoI GGCGCC 1 cut(s) 1024
Sfr274I CTCGAG 1 cut(s) 1250
SinI GGWCC 4 cut(s) 232, 916, 959, 1399
SlaI CTCGAG 1 cut(s) 1250
SmiMI CAYNNNNRTG 3 cut(s) 464, 707, 1121
SmlI CTYRAG 1 cut(s) 1250
SmoI CTYRAG 1 cut(s) 1250
Sse9I AATT 4 cut(s) 199, 579, 969, 1147
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 2 cut(s) 919, 964
SspDI GGCGCC 1 cut(s) 1022
SspI AATATT 4 cut(s) 189, 475, 625, 1309
SspMI CTAG 4 cut(s) 74, 195, 1109, 1405
SstI GAGCTC 1 cut(s) 1344
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 6 cut(s) 251, 613, 1004, 1032, 1350, 1395
StyI CCWWGG 4 cut(s) 10, 527, 899, 1015
TaaI ACNGT 4 cut(s) 329, 358, 805, 995
TaiI ACGT 1 cut(s) 575
TaqI TCGA 2 cut(s) 1251, 1317
TaqII GACCGA 1 cut(s) 737
TasI AATT 4 cut(s) 199, 579, 969, 1147
TatI WGTACW 2 cut(s) 61, 830
TfiI GAWTC 4 cut(s) 15, 248, 763, 1081
Tru1I TTAA 3 cut(s) 378, 741, 1092
Tru9I TTAA 3 cut(s) 378, 741, 1092
TscAI CASTG 4 cut(s) 441, 819, 833, 1144
TseFI GTSAC 2 cut(s) 513, 749
TseI GCWGC 4 cut(s) 49, 306, 883, 1392
Tsp45I GTSAC 2 cut(s) 513, 749
TspDTI ATGAA 6 cut(s) 17, 196, 434, 544, 773, 1276
TspGWI ACGGA 2 cut(s) 929, 974
TspRI CASTG 4 cut(s) 441, 819, 833, 1144
VpaK11BI GGWCC 4 cut(s) 232, 916, 959, 1399
XapI RAATTY 2 cut(s) 969, 1147
XceI RCATGY 5 cut(s) 130, 368, 682, 1099, 1362
XhoI CTCGAG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 4 cut(s) 74, 195, 1109, 1405
ZrmI AGTACT 1 cut(s) 63
Zsp2I ATGCAT 2 cut(s) 128, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.