Rw1G039710

OPT oligopeptide transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004564
Physical Location & Seq
Reverse (-)
67259905 .. 67265980
6076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G039710.1

Sequence Viewer

Length: 1332 bp
ATGGCCGAGGAAGATGTCACCACCAACAACAATGAAGGCCAAGGCAGAGGTGGGGCAGATTTGGGAGAGATTGAAGTTGAGATGCTTGGATCTGGGGTGATTGGAGGTGATGAAGGTGGGGAGGTGGCCATTGAAGGCCACTCCTTGAAAAACTCGATGGAGGAAGTGAGGCTCAACCTTAAGGAGAACAAGGTCGAGGAGTTGGTAAAGACAAAGGCAGGAGGCCTTAGTGATTCTGCAAGTGAGGCGGAGTCAGCCAACGAGGGGCAACCGGGAGGTGCCAAGAAGGATATACTGGACTCGTTTTGTGGAAATAAGAAAAAGGATGTCCATGCAAGGCTTATGAAGAGTTACAAATCAGTGCCAACATTGTGGTTTATCCTCATCCTTGTGATCAATATTGCTCTTATCCTGTTTGCTTGCGTGTATTACAAGGAGTCACTTCAGCTGCCTTGGTGGGGTGCATTGCTTGCTTGTTTCATGGCCGTTGCGTTCACGTACCCAATTGGTGTCATCCAAGCCACCACAAACCAGCAACCAGGTTTGAATATTATCACAGAATATGTGATAGGATACCTTTATCCTGAGCGCCCTGTAGCTAACATGTGCTTCAAAGTGTATGGTTACATAAGTATGGTGCAAGGTCTAAATTTTGTGTCGGATTTTAAGCTCGGTCACTACATGAAGATTCCACCAAGAGCAATGTTCACAGTACAGATGGTAGGAACTGTTGTAGCAGTGGTTGTATACCAGTGTACAGCATGGTGGCTGATGGCAGACATTCCCAACCTCTGTGATACCTCCCTGCTGCCACCGGACAGCCCTTGGACTTGTCCAACGGACCGCGTGTTCTTTGATGCGTCTGTTATCTGGGGTCTTGTTGGACCCCGCAGAATTTTTGGAAACCTCGGTGAATACAGTAACATCAACTGGTTTTTCCTTGGTGGCGCCATTGCCCCGGTGCTTGTTTGGCTTGCACACAGGATTTTTCCCAAACAAAAATGGATTCTTGCTGTTAACATGCCCGTCTTACTAGGTGCCACGGCAATGATGCCCCCTGCCACTGCTTTGAACTTTCAAAGTTGGATATTTGTGGCATTTCTTTCAGGTTTTGTTGTCTTCAGGTACCGGCAAGATTTGTGGAAGAAGTATAACTATGTCGCCTCTGGTGCTCTCGAGGCCGGAACTGCCTTTGTTACGATATTGGTGTTCATTACCTTGCAGTCCAAGAATATTGAACTCGACTGGTGGGGGAACAAAGGAGAGCTCTGCCCCCTGGCTACATGCCCTACTGCGAAAGGGGTTGTCATTGCTGGCTGCCCGGTCCTCTAG

Protein Analysis

443

Amino Acids

48.77

Weight (kDa)

5.57

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 101 - 405 1.6e-78 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1125
AccB1I GGYRCC 4 cut(s) 278, 947, 1037, 1125
AccI GTMKAC 1 cut(s) 747
AccII CGCG 1 cut(s) 846
AciI CCGC 3 cut(s) 248, 844, 889
AclWI GGATC 1 cut(s) 97
AcoI YGGCCR 3 cut(s) 3, 126, 483
AcsI RAATTY 2 cut(s) 649, 894
AcuI CTGAAG 2 cut(s) 428, 1105
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 4 cut(s) 500, 714, 757, 1127
AfiI CCNNNNNNNGG 2 cut(s) 264, 458
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 603
AgsI TTSAA 8 cut(s) 74, 134, 148, 547, 613, 1072, 1079, 1238
AjnI CCWGG 2 cut(s) 538, 1275
AloI GAACNNNNNNTCC 2 cut(s) 833, 865
AluBI AGCT 4 cut(s) 448, 599, 670, 1267
AluI AGCT 4 cut(s) 448, 599, 670, 1267
Alw21I GWGCWC 2 cut(s) 1174, 1269
AlwI GGATC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 1175
AoxI GGCC 7 cut(s) 3, 37, 126, 136, 223, 483, 1179
ApeKI GCWGC 3 cut(s) 448, 808, 1317
ApoI RAATTY 2 cut(s) 649, 894
Asp718I GGTACC 1 cut(s) 1125
AspLEI GCGC 2 cut(s) 591, 950
AspS9I GGNCC 3 cut(s) 841, 884, 1324
AsuC2I CCSGG 3 cut(s) 273, 959, 1322
AsuHPI GGTGA 4 cut(s) 10, 109, 119, 923
AvaI CYCGRG 1 cut(s) 1175
AvaII GGWCC 3 cut(s) 841, 884, 1324
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 4 cut(s) 278, 947, 1037, 1125
BanII GRGCYC 1 cut(s) 1269
BbsI GAAGAC 1 cut(s) 1111
Bbv12I GWGCWC 2 cut(s) 1174, 1269
BbvI GCAGC 3 cut(s) 435, 795, 1304
BccI CCATC 3 cut(s) 151, 712, 766
BceAI ACGGC 2 cut(s) 470, 1059
BciT130I CCWGG 2 cut(s) 540, 1277
BciVI GTATCC 1 cut(s) 566
BclI TGATCA 1 cut(s) 393
BcnI CCSGG 3 cut(s) 273, 959, 1322
BfaI CTAG 2 cut(s) 1034, 1330
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 592, 951
BfrI CTTAAG 1 cut(s) 179
BfuI GTATCC 1 cut(s) 566
BisI GCNGC 3 cut(s) 449, 809, 1318
BlsI GCNGC 3 cut(s) 450, 810, 1319
Bme1390I CCNGG 5 cut(s) 273, 540, 959, 1277, 1322
Bme18I GGWCC 3 cut(s) 841, 884, 1324
BmeT110I CYCGRG 1 cut(s) 1175
BmgT120I GGNCC 3 cut(s) 841, 884, 1324
BmiI GGNNCC 5 cut(s) 280, 886, 949, 1039, 1127
BmrFI CCNGG 5 cut(s) 273, 540, 959, 1277, 1322
BmsI GCATC 3 cut(s) 72, 847, 1041
BpiI GAAGAC 1 cut(s) 1111
Bpu10I CCTNAGC 1 cut(s) 585
BpuMI CCSGG 3 cut(s) 273, 959, 1322
BsaAI YACGTR 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 948
BsaJI CCNNGG 9 cut(s) 6, 40, 452, 824, 907, 940, 957, 1041, 1275
BsaWI WCCGGW 1 cut(s) 814
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 458
Bse118I RCCGGY 1 cut(s) 1128
Bse1I ACTGG 4 cut(s) 300, 751, 935, 1250
Bse3DI GCAATG 5 cut(s) 464, 708, 951, 1053, 1308
BseBI CCWGG 2 cut(s) 540, 1277
BseDI CCNNGG 9 cut(s) 6, 40, 452, 824, 907, 940, 957, 1041, 1275
BseGI GGATG 3 cut(s) 331, 384, 513
BseLI CCNNNNNNNGG 2 cut(s) 264, 458
BseMI GCAATG 5 cut(s) 464, 708, 951, 1053, 1308
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 4 cut(s) 300, 751, 935, 1250
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 435, 795, 1304
Bsh1236I CGCG 1 cut(s) 846
BshFI GGCC 7 cut(s) 5, 39, 128, 138, 225, 485, 1181
BshNI GGYRCC 4 cut(s) 278, 947, 1037, 1125
BsiHKAI GWGCWC 2 cut(s) 1174, 1269
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 6 cut(s) 272, 815, 959, 1129, 1182, 1322
BslI CCNNNNNNNGG 2 cut(s) 264, 458
BsnI GGCC 7 cut(s) 5, 39, 128, 138, 225, 485, 1181
BsoBI CYCGRG 1 cut(s) 1175
Bsp1286I GDGCHC 2 cut(s) 1174, 1269
Bsp1407I TGTACA 1 cut(s) 755
Bsp143I GATC 2 cut(s) 89, 393
BspACI CCGC 3 cut(s) 248, 844, 889
BspANI GGCC 7 cut(s) 5, 39, 128, 138, 225, 485, 1181
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 1 cut(s) 846
BspLI GGNNCC 5 cut(s) 280, 886, 949, 1039, 1127
BspPI GGATC 1 cut(s) 97
BspT107I GGYRCC 4 cut(s) 278, 947, 1037, 1125
BspTI CTTAAG 1 cut(s) 179
BsrDI GCAATG 5 cut(s) 464, 708, 951, 1053, 1308
BsrFI RCCGGY 1 cut(s) 1128
BsrGI TGTACA 1 cut(s) 755
BsrI ACTGG 4 cut(s) 300, 751, 935, 1250
BssAI RCCGGY 1 cut(s) 1128
BssECI CCNNGG 9 cut(s) 6, 40, 452, 824, 907, 940, 957, 1041, 1275
BssMI GATC 2 cut(s) 89, 393
BssNAI GTATAC 1 cut(s) 748
BssNI GRCGYC 1 cut(s) 948
BssT1I CCWWGG 4 cut(s) 40, 452, 824, 940
Bst1107I GTATAC 1 cut(s) 748
Bst2UI CCWGG 2 cut(s) 540, 1277
Bst4CI ACNGT 3 cut(s) 712, 730, 920
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 948
BstAFI CTTAAG 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 470
BstAUI TGTACA 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 498
BstC8I GCNNGC 4 cut(s) 421, 471, 975, 1315
BstDEI CTNAG 2 cut(s) 227, 585
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 3 cut(s) 331, 384, 513
BstFNI CGCG 1 cut(s) 846
BstH2I RGCGCY 2 cut(s) 592, 951
BstHHI GCGC 2 cut(s) 591, 950
BstKTI GATC 2 cut(s) 92, 396
BstMBI GATC 2 cut(s) 89, 393
BstMWI GCNNNNNNNGC 7 cut(s) 245, 254, 470, 970, 1169, 1178, 1187
BstNI CCWGG 2 cut(s) 540, 1277
BstNSI RCATGY 3 cut(s) 607, 1024, 1287
BstSCI CCNGG 5 cut(s) 271, 538, 957, 1275, 1320
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 1 cut(s) 846
BstV1I GCAGC 3 cut(s) 435, 795, 1304
BstV2I GAAGAC 1 cut(s) 1111
BstX2I RGATCY 1 cut(s) 89
BstXI CCANNNNNNTGG 1 cut(s) 372
BstYI RGATCY 1 cut(s) 89
BstZ17I GTATAC 1 cut(s) 748
BsuI GTATCC 1 cut(s) 566
BsuRI GGCC 7 cut(s) 5, 39, 128, 138, 225, 485, 1181
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 3 cut(s) 331, 384, 513
BtsI GCAGTG 2 cut(s) 744, 1062
BtsIMutI CAGTG 4 cut(s) 366, 744, 758, 1062
Cac8I GCNNGC 4 cut(s) 421, 471, 975, 1315
CfoI GCGC 2 cut(s) 591, 950
Cfr10I RCCGGY 1 cut(s) 1128
Cfr13I GGNCC 3 cut(s) 841, 884, 1324
CpoI CGGWCCG 1 cut(s) 841
CseI GACGC 1 cut(s) 849
CsiI ACCWGGT 1 cut(s) 538
Csp6I GTAC 4 cut(s) 499, 713, 756, 1126
CspCI CAANNNNNGTGG 2 cut(s) 1121, 1156
CspI CGGWCCG 1 cut(s) 841
CviAII CATG 7 cut(s) 332, 481, 604, 682, 762, 1021, 1284
CviQI GTAC 4 cut(s) 499, 713, 756, 1126
DdeI CTNAG 2 cut(s) 227, 585
DinI GGCGCC 1 cut(s) 949
DpnI GATC 2 cut(s) 91, 395
DpnII GATC 2 cut(s) 89, 393
EaeI YGGCCR 3 cut(s) 3, 126, 483
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
EciI GGCGGA 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 1267
Eco130I CCWWGG 4 cut(s) 40, 452, 824, 940
Eco147I AGGCCT 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 1269
Eco47I GGWCC 3 cut(s) 841, 884, 1324
Eco53kI GAGCTC 1 cut(s) 1267
Eco57I CTGAAG 2 cut(s) 428, 1105
Eco88I CYCGRG 1 cut(s) 1175
EcoICRI GAGCTC 1 cut(s) 1267
EcoRII CCWGG 2 cut(s) 538, 1275
EcoT14I CCWWGG 4 cut(s) 40, 452, 824, 940
EcoT38I GRGCYC 1 cut(s) 1269
EgeI GGCGCC 1 cut(s) 949
EheI GGCGCC 1 cut(s) 949
ErhI CCWWGG 4 cut(s) 40, 452, 824, 940
FaeI CATG 7 cut(s) 335, 484, 607, 685, 765, 1024, 1287
FatI CATG 7 cut(s) 331, 480, 603, 681, 761, 1020, 1283
FauI CCCGC 1 cut(s) 896
FbaI TGATCA 1 cut(s) 393
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 3 cut(s) 449, 809, 1318
FokI GGATG 3 cut(s) 338, 371, 500
FriOI GRGCYC 1 cut(s) 1269
Fsp4HI GCNGC 3 cut(s) 449, 809, 1318
FspBI CTAG 2 cut(s) 1034, 1330
GlaI GCGC 2 cut(s) 590, 949
GluI GCNGC 3 cut(s) 449, 809, 1318
HaeII RGCGCY 2 cut(s) 592, 951
HaeIII GGCC 7 cut(s) 5, 39, 128, 138, 225, 485, 1181
HapII CCGG 6 cut(s) 272, 815, 959, 1129, 1182, 1322
HgaI GACGC 1 cut(s) 849
HhaI GCGC 2 cut(s) 591, 950
Hin1I GRCGYC 1 cut(s) 948
Hin1II CATG 7 cut(s) 335, 484, 607, 685, 765, 1024, 1287
Hin6I GCGC 2 cut(s) 589, 948
HinP1I GCGC 2 cut(s) 589, 948
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HinfI GANTC 6 cut(s) 233, 251, 299, 437, 688, 1006
HpaI GTTAAC 1 cut(s) 1018
HpaII CCGG 6 cut(s) 272, 815, 959, 1129, 1182, 1322
HphI GGTGA 4 cut(s) 10, 109, 119, 923
Hpy166II GTNNAC 5 cut(s) 495, 708, 748, 756, 1018
Hpy188I TCNGA 1 cut(s) 661
Hpy188III TCNNGA 2 cut(s) 584, 1175
Hpy8I GTNNAC 5 cut(s) 495, 708, 748, 756, 1018
HpyAV CCTTC 4 cut(s) 29, 107, 128, 280
HpyCH4III ACNGT 3 cut(s) 712, 730, 920
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 6 cut(s) 239, 335, 464, 640, 977, 1222
HpyF10VI GCNNNNNNNGC 7 cut(s) 245, 254, 470, 970, 1169, 1178, 1187
HpyF3I CTNAG 2 cut(s) 227, 585
HpySE526I ACGT 1 cut(s) 497
Hsp92I GRCGYC 1 cut(s) 948
Hsp92II CATG 7 cut(s) 335, 484, 607, 685, 765, 1024, 1287
HspAI GCGC 2 cut(s) 589, 948
KasI GGCGCC 1 cut(s) 947
KpnI GGTACC 1 cut(s) 1129
Ksp22I TGATCA 1 cut(s) 393
KspAI GTTAAC 1 cut(s) 1018
Kzo9I GATC 2 cut(s) 89, 393
Lsp1109I GCAGC 3 cut(s) 435, 795, 1304
LweI GCATC 3 cut(s) 72, 847, 1041
MabI ACCWGGT 1 cut(s) 538
MaeI CTAG 2 cut(s) 1034, 1330
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 7 cut(s) 16, 350, 438, 623, 674, 920, 1195
MalI GATC 2 cut(s) 91, 395
MboI GATC 2 cut(s) 89, 393
MboII GAAGA 5 cut(s) 23, 358, 697, 1111, 1156
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 1174, 1269
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 3 cut(s) 504, 649, 894
MluNI TGGCCA 1 cut(s) 128
Mly113I GGCGCC 1 cut(s) 948
MlyI GAGTC 3 cut(s) 260, 293, 446
MmeI TCCRAC 4 cut(s) 639, 860, 862, 1064
Mox20I TGGCCA 1 cut(s) 128
MscI TGGCCA 1 cut(s) 128
MseI TTAA 3 cut(s) 180, 666, 1017
MslI CAYNNNNRTG 3 cut(s) 389, 632, 1046
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 448
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 6 cut(s) 272, 815, 959, 1129, 1182, 1322
MspR9I CCNGG 5 cut(s) 273, 540, 959, 1277, 1322
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 2 cut(s) 540, 1277
MvnI CGCG 1 cut(s) 846
MwoI GCNNNNNNNGC 7 cut(s) 245, 254, 470, 970, 1169, 1178, 1187
NarI GGCGCC 1 cut(s) 948
NciI CCSGG 3 cut(s) 273, 959, 1322
NdeII GATC 2 cut(s) 89, 393
NlaIII CATG 7 cut(s) 335, 484, 607, 685, 765, 1024, 1287
NlaIV GGNNCC 5 cut(s) 280, 886, 949, 1039, 1127
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 3 cut(s) 16, 438, 674
NspI RCATGY 3 cut(s) 607, 1024, 1287
PaeR7I CTCGAG 1 cut(s) 1175
PceI AGGCCT 1 cut(s) 225
PciI ACATGT 1 cut(s) 603
PfeI GAWTC 3 cut(s) 233, 688, 1006
PkrI GCNGC 3 cut(s) 450, 810, 1319
PleI GAGTC 3 cut(s) 259, 293, 445
PluTI GGCGCC 1 cut(s) 951
PpsI GAGTC 3 cut(s) 259, 293, 445
Ppu21I YACGTR 1 cut(s) 498
PscI ACATGT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 1269
Psp6I CCWGG 2 cut(s) 538, 1275
PspGI CCWGG 2 cut(s) 538, 1275
PspN4I GGNNCC 5 cut(s) 280, 886, 949, 1039, 1127
PspPI GGNCC 3 cut(s) 841, 884, 1324
PsuI RGATCY 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 4 cut(s) 500, 714, 757, 1127
RsaNI GTAC 4 cut(s) 499, 713, 756, 1126
RseI CAYNNNNRTG 3 cut(s) 389, 632, 1046
Rsr2I CGGWCCG 1 cut(s) 841
RsrII CGGWCCG 1 cut(s) 841
SacI GAGCTC 1 cut(s) 1269
SaqAI TTAA 3 cut(s) 180, 666, 1017
SatI GCNGC 3 cut(s) 449, 809, 1318
Sau3AI GATC 2 cut(s) 89, 393
Sau96I GGNCC 3 cut(s) 841, 884, 1324
SchI GAGTC 3 cut(s) 260, 293, 446
ScrFI CCNGG 5 cut(s) 273, 540, 959, 1277, 1322
SduI GDGCHC 2 cut(s) 1174, 1269
SexAI ACCWGGT 1 cut(s) 538
SfaNI GCATC 3 cut(s) 72, 847, 1041
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 1 cut(s) 949
Sfr274I CTCGAG 1 cut(s) 1175
SinI GGWCC 3 cut(s) 841, 884, 1324
SlaI CTCGAG 1 cut(s) 1175
SmiMI CAYNNNNRTG 3 cut(s) 389, 632, 1046
SmlI CTYRAG 2 cut(s) 179, 1175
SmoI CTYRAG 2 cut(s) 179, 1175
Sse9I AATT 3 cut(s) 504, 649, 894
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 3 cut(s) 248, 844, 889
SspDI GGCGCC 1 cut(s) 947
SspI AATATT 3 cut(s) 400, 550, 1234
SspMI CTAG 2 cut(s) 1034, 1330
SstI GAGCTC 1 cut(s) 1269
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 5 cut(s) 271, 538, 957, 1275, 1320
StyI CCWWGG 4 cut(s) 40, 452, 824, 940
TaaI ACNGT 3 cut(s) 712, 730, 920
TaiI ACGT 1 cut(s) 500
TaqI TCGA 4 cut(s) 155, 195, 1176, 1242
TaqII GACCGA 1 cut(s) 662
TasI AATT 3 cut(s) 504, 649, 894
TatI WGTACW 2 cut(s) 712, 755
TfiI GAWTC 3 cut(s) 233, 688, 1006
Tru1I TTAA 3 cut(s) 180, 666, 1017
Tru9I TTAA 3 cut(s) 180, 666, 1017
TscAI CASTG 4 cut(s) 366, 744, 758, 1069
TseFI GTSAC 3 cut(s) 16, 438, 674
TseI GCWGC 3 cut(s) 448, 808, 1317
Tsp45I GTSAC 3 cut(s) 16, 438, 674
TspDTI ATGAA 6 cut(s) 48, 126, 359, 469, 698, 1201
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 4 cut(s) 366, 744, 758, 1069
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 3 cut(s) 841, 884, 1324
XapI RAATTY 2 cut(s) 649, 894
XceI RCATGY 3 cut(s) 607, 1024, 1287
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 1175
XmiI GTMKAC 1 cut(s) 747
XspI CTAG 2 cut(s) 1034, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.