RchiOBHm_Chr2g0107221

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014828
Physical Location & Seq
Forward (+)
18548532 .. 18549435
904 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48125

Sequence Viewer

Length: 843 bp
ATGAGGCGCATAGCATCTGGGAGATTAGTCCCAAGGAACTGTTCCTGTCCTTCAGCAATGGAAACAAGAGCTGACCTCAAGGGAGTCAAATCCCAAGTCCAGAATCTGGTTGAAGAGTTACATGGCACTTGTGTAAATACTCAAAGAGAAGCAACACTTCAGCTCCGACTACTCGCAAAGCATAACATGAACAATCGGATTGTTATTGCAAACTGTGGAGCCATAAGCTTTTTGGTGGGTTTGCTAAGCTCAACTGACAAAAGGGTACAAGAAAATGCTGTCACAGCACTTCTTAACTTGTCGATCAATAATGACAACGAGATCAGAATTGCTACTGCTGCCAAGGCAATTGAGTCTGTGGTTCATGTGCTTGAGAATGGGAGTGATGAGGTCAAGGAGAACTCAGCTGCCACATTATTTAGCCTTTCGATGACCTGGGAAAACAAGGTTCGGATTGGAAGGTCCAAGGCTATTAGGCCTCTAGTTGAGTTACTAGGGAATGGGAGTCTTAGAGGGGAAAAAAGATGCAGCTACAGCATTTACTTGGTGGAGTTTGTGATGAATCCAGAAGCTGGAATAGTTTACCAGACTATTGCTGTTTTGGCTGCTTTTTGTACAAGTCATGAAGGGAGGATAGCAATTGTTGAGGAAGGTGGGGTTCCTGTTCTGGTTGAGGTTTTGAAGTTAGGTTCCGAAAGAGGCAAGGAGAATGCAGCTGCTGCTCTATTACAGCTCTGCACAAGTAGTGATGAACAATGTAGTATGGTGGTTGATAAAGGAGTTGTTCCATCTTTGGTGGCAATGTCACAATCTGGCACCCCAAGGGCCAAAGAAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

280

Amino Acids

30.22

Weight (kDa)

8.11

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 33 - 156 4.1e-08 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 33 - 173 2.5e-08 ARMC5
Arm PF00514 64 - 101 1.2e-09 Armadillo/beta-catenin-like repeat
ARM_PUB PF25598 182 - 273 6.8e-08 Plant U-box protein ARM repeat region
Arm PF00514 208 - 246 5.1e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 815
AccB7I CCANNNNNTGG 2 cut(s) 106, 572
AcuI CTGAAG 2 cut(s) 36, 143
AfaI GTAC 2 cut(s) 267, 616
AfiI CCNNNNNNNGG 2 cut(s) 106, 572
AgsI TTSAA 2 cut(s) 113, 682
AjnI CCWGG 1 cut(s) 434
AjuI GAANNNNNNNTTGG 2 cut(s) 25, 57
AluBI AGCT 9 cut(s) 71, 163, 228, 249, 407, 531, 572, 716, 733
AluI AGCT 9 cut(s) 71, 163, 228, 249, 407, 531, 572, 716, 733
AlwNI CAGNNNCTG 3 cut(s) 106, 572, 719
AoxI GGCC 2 cut(s) 476, 825
ApeKI GCWGC 7 cut(s) 338, 407, 528, 605, 713, 716, 719
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 462, 825
AvaII GGWCC 1 cut(s) 462
BanI GGYRCC 1 cut(s) 815
BbvI GCAGC 7 cut(s) 325, 394, 540, 592, 703, 706, 725
BccI CCATC 1 cut(s) 796
BciT130I CCWGG 1 cut(s) 436
BfaI CTAG 2 cut(s) 482, 494
BfmI CTRYAG 1 cut(s) 532
BisI GCNGC 7 cut(s) 339, 408, 529, 606, 714, 717, 720
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 7 cut(s) 340, 409, 530, 607, 715, 718, 721
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 1 cut(s) 462
BmgT120I GGNCC 2 cut(s) 462, 825
BmiI GGNNCC 4 cut(s) 220, 660, 691, 817
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 2 cut(s) 23, 515
BplI GAGNNNNNCTC 2 cut(s) 60, 92
Bpu1102I GCTNAGC 1 cut(s) 245
BpuEI CTTGAG 2 cut(s) 62, 392
BsaJI CCNNGG 5 cut(s) 32, 342, 435, 465, 821
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 572
Bse3DI GCAATG 2 cut(s) 63, 807
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 5 cut(s) 32, 342, 435, 465, 821
BseLI CCNNNNNNNGG 2 cut(s) 106, 572
BseMI GCAATG 2 cut(s) 63, 807
BseMII CTCAG 1 cut(s) 417
BseXI GCAGC 7 cut(s) 325, 394, 540, 592, 703, 706, 725
BsgI GTGCAG 1 cut(s) 721
BshFI GGCC 2 cut(s) 478, 827
BshNI GGYRCC 1 cut(s) 815
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 2 cut(s) 106, 572
BsmFI GGGAC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 2 cut(s) 478, 827
Bsp1407I TGTACA 1 cut(s) 614
Bsp143I GATC 2 cut(s) 303, 321
Bsp1720I GCTNAGC 1 cut(s) 245
BspANI GGCC 2 cut(s) 478, 827
BspCNI CTCAG 1 cut(s) 416
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 4 cut(s) 220, 660, 691, 817
BspT107I GGYRCC 1 cut(s) 815
BsrDI GCAATG 2 cut(s) 63, 807
BsrGI TGTACA 1 cut(s) 614
BssECI CCNNGG 5 cut(s) 32, 342, 435, 465, 821
BssMI GATC 2 cut(s) 303, 321
BssT1I CCWWGG 4 cut(s) 32, 342, 465, 821
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 41, 215
Bst6I CTCTTC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 614
BstDEI CTNAG 3 cut(s) 245, 403, 509
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 2 cut(s) 306, 324
BstMBI GATC 2 cut(s) 303, 321
BstMWI GCNNNNNNNGC 6 cut(s) 284, 338, 344, 534, 602, 719
BstNI CCWGG 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 532
BstV1I GCAGC 7 cut(s) 325, 394, 540, 592, 703, 706, 725
BsuRI GGCC 2 cut(s) 478, 827
CaiI CAGNNNCTG 3 cut(s) 106, 572, 719
CciI TCATGA 1 cut(s) 622
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 462, 825
Csp6I GTAC 2 cut(s) 266, 615
CviAII CATG 4 cut(s) 122, 187, 365, 623
CviQI GTAC 2 cut(s) 266, 615
DdeI CTNAG 3 cut(s) 245, 403, 509
DpnI GATC 2 cut(s) 305, 323
DpnII GATC 2 cut(s) 303, 321
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 4 cut(s) 32, 342, 465, 821
Eco147I AGGCCT 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 462
Eco57I CTGAAG 2 cut(s) 36, 143
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 4 cut(s) 32, 342, 465, 821
ErhI CCWWGG 4 cut(s) 32, 342, 465, 821
FaeI CATG 4 cut(s) 125, 190, 368, 626
FaiI YATR 9 cut(s) 11, 123, 183, 188, 224, 366, 624, 764, 841
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 14
FatI CATG 4 cut(s) 121, 186, 364, 622
Fnu4HI GCNGC 7 cut(s) 339, 408, 529, 606, 714, 717, 720
Fsp4HI GCNGC 7 cut(s) 339, 408, 529, 606, 714, 717, 720
FspBI CTAG 2 cut(s) 482, 494
GlaI GCGC 1 cut(s) 8
GluI GCNGC 7 cut(s) 339, 408, 529, 606, 714, 717, 720
HaeIII GGCC 2 cut(s) 478, 827
HhaI GCGC 1 cut(s) 9
Hin1II CATG 4 cut(s) 125, 190, 368, 626
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 226
HinfI GANTC 5 cut(s) 84, 103, 353, 505, 562
Hpy166II GTNNAC 1 cut(s) 583
Hpy188I TCNGA 5 cut(s) 167, 198, 326, 453, 694
Hpy188III TCNNGA 3 cut(s) 100, 566, 623
Hpy8I GTNNAC 1 cut(s) 583
HpyAV CCTTC 4 cut(s) 60, 453, 620, 644
HpyCH4III ACNGT 2 cut(s) 41, 215
HpyCH4V TGCA 4 cut(s) 209, 528, 713, 738
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 338, 344, 534, 602, 719
HpyF3I CTNAG 3 cut(s) 245, 403, 509
Hsp92II CATG 4 cut(s) 125, 190, 368, 626
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 2 cut(s) 303, 321
LmnI GCTCC 2 cut(s) 168, 218
Lsp1109I GCAGC 7 cut(s) 325, 394, 540, 592, 703, 706, 725
LweI GCATC 2 cut(s) 23, 515
MaeI CTAG 2 cut(s) 482, 494
MaeIII GTNAC 4 cut(s) 117, 280, 489, 804
MalI GATC 2 cut(s) 305, 323
MboI GATC 2 cut(s) 303, 321
MboII GAAGA 1 cut(s) 125
MfeI CAATTG 2 cut(s) 348, 639
MluCI AATT 3 cut(s) 327, 348, 639
MlyI GAGTC 3 cut(s) 93, 362, 514
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 8 cut(s) 86, 382, 489, 506, 624, 640, 667, 692
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 2 cut(s) 407, 716
MspR9I CCNGG 1 cut(s) 436
MunI CAATTG 2 cut(s) 348, 639
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 1 cut(s) 436
MwoI GCNNNNNNNGC 6 cut(s) 284, 338, 344, 534, 602, 719
NdeII GATC 2 cut(s) 303, 321
NlaIII CATG 4 cut(s) 125, 190, 368, 626
NlaIV GGNNCC 4 cut(s) 220, 660, 691, 817
NmuCI GTSAC 2 cut(s) 280, 804
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 478
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 2 cut(s) 103, 562
PflMI CCANNNNNTGG 2 cut(s) 106, 572
PkrI GCNGC 7 cut(s) 340, 409, 530, 607, 715, 718, 721
PleI GAGTC 3 cut(s) 92, 361, 513
PpsI GAGTC 3 cut(s) 92, 361, 513
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 4 cut(s) 220, 660, 691, 817
PspPI GGNCC 2 cut(s) 462, 825
PstNI CAGNNNCTG 3 cut(s) 106, 572, 719
PvuII CAGCTG 2 cut(s) 407, 716
RsaI GTAC 2 cut(s) 267, 616
RsaNI GTAC 2 cut(s) 266, 615
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 7 cut(s) 339, 408, 529, 606, 714, 717, 720
Sau3AI GATC 2 cut(s) 303, 321
Sau96I GGNCC 2 cut(s) 462, 825
SchI GAGTC 3 cut(s) 93, 362, 514
ScrFI CCNGG 1 cut(s) 436
SfaNI GCATC 2 cut(s) 23, 515
SfcI CTRYAG 1 cut(s) 532
SinI GGWCC 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 77, 371
SmoI CTYRAG 2 cut(s) 77, 371
Sse9I AATT 3 cut(s) 327, 348, 639
SseBI AGGCCT 1 cut(s) 478
SspMI CTAG 2 cut(s) 482, 494
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 434
StyI CCWWGG 4 cut(s) 32, 342, 465, 821
TaaI ACNGT 2 cut(s) 41, 215
TaqI TCGA 2 cut(s) 302, 428
TasI AATT 3 cut(s) 327, 348, 639
TatI WGTACW 1 cut(s) 614
TfiI GAWTC 2 cut(s) 103, 562
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseFI GTSAC 2 cut(s) 280, 804
TseI GCWGC 7 cut(s) 338, 407, 528, 605, 713, 716, 719
Tsp45I GTSAC 2 cut(s) 280, 804
TspDTI ATGAA 5 cut(s) 203, 353, 575, 639, 765
Van91I CCANNNNNTGG 2 cut(s) 106, 572
VpaK11BI GGWCC 1 cut(s) 462
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XspI CTAG 2 cut(s) 482, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.