Rw2G015160

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014828
Physical Location & Seq
Forward (+)
17494841 .. 17502807
7967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015160.1

Sequence Viewer

Length: 645 bp
ATGGAAACAAGAGCAAACCTCAAGGGAGTCAACTCCCAAGTCCAGAATCTGGTTGAAGAGTTACATGGCACTTGTGTAAATACTCAAAGAGAAGCAACACTTCAGCTCCGACTACTCGCAAAGCATAACATGAACAATCGGATTGTTATTGCAAACTGTGGAGCCATAAGCTTTTTGGTGGGTTTGCGAAGCTCAACTGACAAAAGGGTACAAGAAAATGCTGTCACGGCACTTCTTAATTTGTCGATCAATAATGACAACAAGATCAGAATTGCTACTGCTGCCAAGGCAATTGAGTCTCTGGTTCATGTGCTTGAGAATAGGAGTGATGAGGCCAAGGAGAACTCAGCTGCCACATTATTTAGCCTTTCGATGACCTGGGAAAAAAAGACTATTGCTGTTTTGGCTGCTTTTTGTACAAGTCATGAAGGGAGGATAGCAATTGTTGAGGAAGGTGGGGTTCCTGTTCTGGTTGAGGTTTTGAAGTTAGGATTCGAAAGAGGCAAGGAGAATGCAGCTGCTGCTCTATTACAGCTCTACACAAATAGTGAAGAACAATGTAGTATGGTGGCTGATAAAGGAGTTGTTCCATCTTTGGTGGCAATGTCACAATCCGGCACCCCTAGGGCCAAAGAAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

214

Amino Acids

23.15

Weight (kDa)

6.91

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arm PF00514 45 - 82 5.9e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 617
AccB7I CCANNNNNTGG 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 2 cut(s) 210, 418
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 2 cut(s) 56, 484
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 6 cut(s) 106, 171, 192, 350, 518, 535
AluI AGCT 6 cut(s) 106, 171, 192, 350, 518, 535
Alw26I GTCTC 1 cut(s) 303
AlwNI CAGNNNCTG 2 cut(s) 49, 521
AoxI GGCC 2 cut(s) 333, 627
ApeKI GCWGC 6 cut(s) 281, 350, 407, 515, 518, 521
AspA2I CCTAGG 1 cut(s) 623
AspS9I GGNCC 1 cut(s) 627
AsuII TTCGAA 1 cut(s) 495
AvrII CCTAGG 1 cut(s) 623
BanI GGYRCC 1 cut(s) 617
BbvI GCAGC 6 cut(s) 268, 337, 394, 505, 508, 527
BccI CCATC 1 cut(s) 598
BceAI ACGGC 1 cut(s) 243
BciT130I CCWGG 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 1 cut(s) 624
BisI GCNGC 6 cut(s) 282, 351, 408, 516, 519, 522
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 6 cut(s) 283, 352, 409, 517, 520, 523
Bme1390I CCNGG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 627
BmiI GGNNCC 3 cut(s) 163, 462, 619
BmrFI CCNGG 1 cut(s) 379
BplI GAGNNNNNCTC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 5, 335
BsaJI CCNNGG 4 cut(s) 285, 336, 378, 623
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 609
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 4 cut(s) 285, 336, 378, 623
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 1 cut(s) 360
BseXI GCAGC 6 cut(s) 268, 337, 394, 505, 508, 527
BshFI GGCC 2 cut(s) 335, 629
BshNI GGYRCC 1 cut(s) 617
BsiSI CCGG 1 cut(s) 615
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 2 cut(s) 335, 629
Bsp119I TTCGAA 1 cut(s) 495
Bsp1407I TGTACA 1 cut(s) 416
Bsp143I GATC 2 cut(s) 246, 264
BspANI GGCC 2 cut(s) 335, 629
BspCNI CTCAG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 163, 462, 619
BspT104I TTCGAA 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 609
BsrGI TGTACA 1 cut(s) 416
BssECI CCNNGG 4 cut(s) 285, 336, 378, 623
BssMI GATC 2 cut(s) 246, 264
BssT1I CCWWGG 3 cut(s) 285, 336, 623
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 521
BstAUI TGTACA 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 346
BstKTI GATC 2 cut(s) 249, 267
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 2 cut(s) 246, 264
BstMWI GCNNNNNNNGC 5 cut(s) 227, 281, 287, 404, 521
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstV1I GCAGC 6 cut(s) 268, 337, 394, 505, 508, 527
BsuRI GGCC 2 cut(s) 335, 629
CaiI CAGNNNCTG 2 cut(s) 49, 521
CciI TCATGA 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 627
Csp6I GTAC 2 cut(s) 209, 417
CviAII CATG 4 cut(s) 65, 130, 308, 425
CviQI GTAC 2 cut(s) 209, 417
DdeI CTNAG 1 cut(s) 346
DpnI GATC 2 cut(s) 248, 266
DpnII GATC 2 cut(s) 246, 264
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 3 cut(s) 285, 336, 623
Eco57I CTGAAG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 3 cut(s) 285, 336, 623
ErhI CCWWGG 3 cut(s) 285, 336, 623
FaeI CATG 4 cut(s) 68, 133, 311, 428
FaiI YATR 8 cut(s) 66, 126, 131, 167, 309, 426, 566, 643
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 4 cut(s) 64, 129, 307, 424
Fnu4HI GCNGC 6 cut(s) 282, 351, 408, 516, 519, 522
Fsp4HI GCNGC 6 cut(s) 282, 351, 408, 516, 519, 522
FspBI CTAG 1 cut(s) 624
GluI GCNGC 6 cut(s) 282, 351, 408, 516, 519, 522
HaeIII GGCC 2 cut(s) 335, 629
HapII CCGG 1 cut(s) 615
Hin1II CATG 4 cut(s) 68, 133, 311, 428
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 4 cut(s) 27, 46, 296, 492
HpaII CCGG 1 cut(s) 615
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 110, 141, 269
Hpy188III TCNNGA 2 cut(s) 43, 425
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 422, 446
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 152, 515
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 281, 287, 404, 521
HpyF3I CTNAG 1 cut(s) 346
Hsp92II CATG 4 cut(s) 68, 133, 311, 428
Kzo9I GATC 2 cut(s) 246, 264
LmnI GCTCC 2 cut(s) 111, 161
LpnPI CCDG 8 cut(s) 35, 56, 287, 364, 391, 455, 477, 628
Lsp1109I GCAGC 6 cut(s) 268, 337, 394, 505, 508, 527
MaeI CTAG 1 cut(s) 624
MaeIII GTNAC 3 cut(s) 60, 223, 606
MalI GATC 2 cut(s) 248, 266
MboI GATC 2 cut(s) 246, 264
MboII GAAGA 2 cut(s) 68, 563
MfeI CAATTG 2 cut(s) 291, 441
MluCI AATT 4 cut(s) 238, 270, 291, 441
MlyI GAGTC 2 cut(s) 36, 305
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 29, 325, 426, 442, 469, 494
MseI TTAA 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 350, 518
MspI CCGG 1 cut(s) 615
MspR9I CCNGG 1 cut(s) 379
MunI CAATTG 2 cut(s) 291, 441
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 5 cut(s) 227, 281, 287, 404, 521
NdeII GATC 2 cut(s) 246, 264
NlaIII CATG 4 cut(s) 68, 133, 311, 428
NlaIV GGNNCC 3 cut(s) 163, 462, 619
NmuCI GTSAC 2 cut(s) 223, 606
NspV TTCGAA 1 cut(s) 495
PagI TCATGA 1 cut(s) 424
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 46, 492
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 6 cut(s) 283, 352, 409, 517, 520, 523
PleI GAGTC 2 cut(s) 35, 304
PpsI GAGTC 2 cut(s) 35, 304
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 163, 462, 619
PspPI GGNCC 1 cut(s) 627
PstNI CAGNNNCTG 2 cut(s) 49, 521
PvuII CAGCTG 2 cut(s) 350, 518
RsaI GTAC 2 cut(s) 210, 418
RsaNI GTAC 2 cut(s) 209, 417
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 6 cut(s) 282, 351, 408, 516, 519, 522
Sau3AI GATC 2 cut(s) 246, 264
Sau96I GGNCC 1 cut(s) 627
SchI GAGTC 2 cut(s) 36, 305
ScrFI CCNGG 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 495
SmlI CTYRAG 2 cut(s) 20, 314
SmoI CTYRAG 2 cut(s) 20, 314
Sse9I AATT 4 cut(s) 238, 270, 291, 441
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 3 cut(s) 285, 336, 623
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 245, 371, 495
TasI AATT 4 cut(s) 238, 270, 291, 441
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 2 cut(s) 46, 492
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseFI GTSAC 2 cut(s) 223, 606
TseI GCWGC 6 cut(s) 281, 350, 407, 515, 518, 521
Tsp45I GTSAC 2 cut(s) 223, 606
TspDTI ATGAA 3 cut(s) 146, 296, 441
Van91I CCANNNNNTGG 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.