Rmu_ssc0000269.1_g000001

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000269.1
OG0014828
Physical Location & Seq
Forward (+)
1 .. 1381
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000269.1_g000001.1.cds

Sequence Viewer

Length: 1103 bp
tagatgccaatgggtccttacagacatcaggcaacctgtcaggctacagcagtgatgcttctggggagtttagatcaggaccaccggctgctacccctcaacacagagaaactgagttgccaaccagattgcaggaggcaagacctcgaaacccaatgtggcgccgacctgctgggaacttaatcccaaggatagtttttactccatccactgaaataagacctgacctttctggactagaagcccaagttcgaactctggtagaggacttgaaaagcacttcacttgatactcaaagagaagcaacatatgaactccggatgcttgcaaagcataatatggataatcggattgttattgcaaactgtggggccattaccttgttggtggaattactccgttctacagattcaagggtccaggaaaattctgtcacagcacttcttaacttgtcgatcaatgataacaacaaaactttaattgctactgctaacgcaattgaacctcttattcatgtccttgagacagggagtgctgaggccaaggagaactcagccgccactctgtttagcctttcggtgattgaggataacaaggttcgtattggaaggtcaggggctattaggcctctagttgatctattggggaatgggactcctagagggaggaaagatgcagccacggctttgtttaatttgtcaatattccatgagaacaaaggtcggattgttcaagctggtgctgtaaagtacttggtggagttaatggacccagctgctggaatggttgacaaggcagttgctgttttggctaatctttctacaattcctgaaggaaggacggcaattggtcaggagggtgggatccctgttctagttgaggtggttgagttgggttctgctagagggaaggagaatgcagctgctgctcttttacagctctgcacaaatagtaataggtattgcagtatggtgctccaagaaggagctgtcccgccgttagtcgtattgtcacaatctggcaccccaagggccagagaaaaggctcagacactacttagatacttcagagaccatagacatggtagtgccggtagaggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

366

Amino Acids

39.34

Weight (kDa)

8.46

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 177
AccB1I GGYRCC 2 cut(s) 161, 1021
AccB7I CCANNNNNTGG 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 2 cut(s) 557, 994
AclWI GGATC 2 cut(s) 858, 871
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 2 cut(s) 851, 1050
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 751
AfiI CCNNNNNNNGG 1 cut(s) 778
AgsI TTSAA 4 cut(s) 273, 413, 502, 733
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 5 cut(s) 736, 775, 922, 939, 988
AluI AGCT 5 cut(s) 736, 775, 922, 939, 988
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 2 cut(s) 517, 1064
AlwI GGATC 2 cut(s) 858, 871
AlwNI CAGNNNCTG 3 cut(s) 778, 802, 925
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 4 cut(s) 371, 539, 625, 1031
ApeKI GCWGC 6 cut(s) 88, 676, 775, 919, 922, 925
ApoI RAATTY 1 cut(s) 426
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 6 cut(s) 14, 79, 371, 417, 768, 1031
AsuHPI GGTGA 1 cut(s) 591
AsuII TTCGAA 1 cut(s) 252
AvaII GGWCC 4 cut(s) 14, 79, 417, 768
BamHI GGATCC 1 cut(s) 863
BanI GGYRCC 2 cut(s) 161, 1021
Bbv12I GWGCWC 1 cut(s) 977
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 6 cut(s) 75, 688, 762, 909, 912, 931
BccI CCATC 1 cut(s) 213
BceAI ACGGC 3 cut(s) 698, 857, 981
BciT130I CCWGG 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 517, 1064
BfaI CTAG 5 cut(s) 238, 631, 659, 874, 902
BfmI CTRYAG 2 cut(s) 45, 404
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 177
BisI GCNGC 7 cut(s) 89, 557, 677, 776, 920, 923, 926
BlsI GCNGC 7 cut(s) 90, 558, 678, 777, 921, 924, 927
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 4 cut(s) 14, 79, 417, 768
BmgT120I GGNCC 6 cut(s) 14, 79, 371, 417, 768, 1031
BmiI GGNNCC 7 cut(s) 15, 163, 372, 418, 770, 865, 1023
BmrFI CCNGG 1 cut(s) 421
BmsI GCATC 3 cut(s) 45, 311, 663
Bpu10I CCTNAGC 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 1064
BsaJI CCNNGG 4 cut(s) 187, 542, 680, 1027
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 1 cut(s) 778
Bse118I RCCGGY 2 cut(s) 84, 1090
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 4 cut(s) 187, 542, 680, 1027
BseGI GGATG 2 cut(s) 205, 326
BseLI CCNNNNNNNGG 1 cut(s) 778
BseMII CTCAG 4 cut(s) 104, 527, 566, 1060
BseXI GCAGC 6 cut(s) 75, 688, 762, 909, 912, 931
BseYI CCCAGC 2 cut(s) 172, 771
BsgI GTGCAG 1 cut(s) 927
BshFI GGCC 4 cut(s) 373, 541, 627, 1033
BshNI GGYRCC 2 cut(s) 161, 1021
BsiHKAI GWGCWC 1 cut(s) 977
BsiSI CCGG 3 cut(s) 85, 318, 1091
BslFI GGGAC 2 cut(s) 666, 976
BslI CCNNNNNNNGG 1 cut(s) 778
BsmAI GTCTC 2 cut(s) 517, 1064
BsmFI GGGAC 2 cut(s) 666, 976
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 4 cut(s) 373, 541, 627, 1033
Bso31I GGTCTC 1 cut(s) 1064
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 977
Bsp13I TCCGGA 1 cut(s) 317
Bsp143I GATC 4 cut(s) 73, 455, 636, 863
BspACI CCGC 2 cut(s) 557, 994
BspANI GGCC 4 cut(s) 373, 541, 627, 1033
BspCNI CTCAG 4 cut(s) 105, 528, 565, 1059
BspEI TCCGGA 1 cut(s) 317
BspLI GGNNCC 7 cut(s) 15, 163, 372, 418, 770, 865, 1023
BspMI ACCTGC 1 cut(s) 177
BspPI GGATC 2 cut(s) 858, 871
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 2 cut(s) 161, 1021
BspTNI GGTCTC 1 cut(s) 1064
BsrFI RCCGGY 2 cut(s) 84, 1090
BssAI RCCGGY 2 cut(s) 84, 1090
BssECI CCNNGG 4 cut(s) 187, 542, 680, 1027
BssMI GATC 4 cut(s) 73, 455, 636, 863
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 3 cut(s) 187, 542, 1027
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 1 cut(s) 367
BstACI GRCGYC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 925
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 5 cut(s) 113, 536, 552, 1046, 1057
BstDSI CCRYGG 1 cut(s) 680
BstF5I GGATG 2 cut(s) 205, 326
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 4 cut(s) 76, 458, 639, 866
BstMAI GTCTC 2 cut(s) 517, 1064
BstMBI GATC 4 cut(s) 73, 455, 636, 863
BstMWI GCNNNNNNNGC 4 cut(s) 330, 682, 808, 925
BstNI CCWGG 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 2 cut(s) 45, 404
BstV1I GCAGC 6 cut(s) 75, 688, 762, 909, 912, 931
BstX2I RGATCY 1 cut(s) 863
BstXI CCANNNNNNTGG 1 cut(s) 1081
BstYI RGATCY 1 cut(s) 863
BsuRI GGCC 4 cut(s) 373, 541, 627, 1033
BtgI CCRYGG 1 cut(s) 680
BtsCI GGATG 2 cut(s) 205, 326
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 57, 209
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 326
CaiI CAGNNNCTG 3 cut(s) 778, 802, 925
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 2 cut(s) 84, 1090
Cfr13I GGNCC 6 cut(s) 14, 79, 371, 417, 768, 1031
Csp6I GTAC 1 cut(s) 750
CviAII CATG 3 cut(s) 514, 709, 1081
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 5 cut(s) 113, 536, 552, 1046, 1057
DinI GGCGCC 1 cut(s) 163
DpnI GATC 4 cut(s) 75, 457, 638, 865
DpnII GATC 4 cut(s) 73, 455, 636, 863
Eco130I CCWWGG 3 cut(s) 187, 542, 1027
Eco147I AGGCCT 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 1064
Eco47I GGWCC 4 cut(s) 14, 79, 417, 768
Eco57I CTGAAG 2 cut(s) 851, 1050
EcoO109I RGGNCCY 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 3 cut(s) 187, 542, 1027
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 3 cut(s) 187, 542, 1027
FaeI CATG 3 cut(s) 517, 712, 1084
FaiI YATR 9 cut(s) 309, 311, 335, 340, 515, 710, 970, 1076, 1082
FaqI GGGAC 2 cut(s) 666, 976
FatI CATG 3 cut(s) 513, 708, 1080
FauI CCCGC 1 cut(s) 1001
FauNDI CATATG 1 cut(s) 309
Fnu4HI GCNGC 7 cut(s) 89, 557, 677, 776, 920, 923, 926
FokI GGATG 2 cut(s) 192, 333
Fsp4HI GCNGC 7 cut(s) 89, 557, 677, 776, 920, 923, 926
FspBI CTAG 5 cut(s) 238, 631, 659, 874, 902
GlaI GCGC 1 cut(s) 163
GluI GCNGC 7 cut(s) 89, 557, 677, 776, 920, 923, 926
GsaI CCCAGC 2 cut(s) 176, 775
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 373, 541, 627, 1033
HapII CCGG 3 cut(s) 85, 318, 1091
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 3 cut(s) 517, 712, 1084
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 2 cut(s) 409, 654
HpaII CCGG 3 cut(s) 85, 318, 1091
HphI GGTGA 1 cut(s) 591
Hpy166II GTNNAC 1 cut(s) 789
Hpy188I TCNGA 4 cut(s) 350, 725, 1049, 1069
Hpy188III TCNNGA 5 cut(s) 77, 233, 318, 829, 853
Hpy8I GTNNAC 1 cut(s) 789
HpyAV CCTTC 5 cut(s) 602, 826, 830, 903, 976
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4V TGCA 7 cut(s) 132, 328, 361, 676, 919, 944, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 330, 682, 808, 925
HpyF3I CTNAG 5 cut(s) 113, 536, 552, 1046, 1057
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 3 cut(s) 517, 712, 1084
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 317
Kzo9I GATC 4 cut(s) 73, 455, 636, 863
LmnI GCTCC 2 cut(s) 980, 985
Lsp1109I GCAGC 6 cut(s) 75, 688, 762, 909, 912, 931
LweI GCATC 3 cut(s) 45, 311, 663
MaeI CTAG 5 cut(s) 238, 631, 659, 874, 902
MaeIII GTNAC 2 cut(s) 432, 1010
MalI GATC 4 cut(s) 75, 457, 638, 865
MboI GATC 4 cut(s) 73, 455, 636, 863
MfeI CAATTG 2 cut(s) 497, 845
MflI RGATCY 1 cut(s) 863
MhlI GDGCHC 1 cut(s) 977
MluCI AATT 7 cut(s) 391, 426, 479, 497, 693, 824, 845
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 648
MmeI TCCRAC 1 cut(s) 703
MroI TCCGGA 1 cut(s) 317
MseI TTAA 5 cut(s) 181, 446, 478, 692, 763
MslI CAYNNNNRTG 2 cut(s) 1079, 1085
MspA1I CMGCKG 2 cut(s) 775, 922
MspI CCGG 3 cut(s) 85, 318, 1091
MspR9I CCNGG 1 cut(s) 421
MunI CAATTG 2 cut(s) 497, 845
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 1 cut(s) 421
MwoI GCNNNNNNNGC 4 cut(s) 330, 682, 808, 925
NarI GGCGCC 1 cut(s) 162
NdeI CATATG 1 cut(s) 309
NdeII GATC 4 cut(s) 73, 455, 636, 863
NlaIII CATG 3 cut(s) 517, 712, 1084
NlaIV GGNNCC 7 cut(s) 15, 163, 372, 418, 770, 865, 1023
NmuCI GTSAC 2 cut(s) 432, 1010
NspV TTCGAA 1 cut(s) 252
PceI AGGCCT 1 cut(s) 627
PctI GAATGC 1 cut(s) 921
PfeI GAWTC 1 cut(s) 409
PflMI CCANNNNNTGG 1 cut(s) 778
PfoI TCCNGGA 1 cut(s) 419
PkrI GCNGC 7 cut(s) 90, 558, 678, 777, 921, 924, 927
PleI GAGTC 1 cut(s) 648
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 648
PpuMI RGGWCCY 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 419
PspFI CCCAGC 2 cut(s) 172, 771
PspGI CCWGG 1 cut(s) 419
PspN4I GGNNCC 7 cut(s) 15, 163, 372, 418, 770, 865, 1023
PspPI GGNCC 6 cut(s) 14, 79, 371, 417, 768, 1031
PspPPI RGGWCCY 1 cut(s) 14
PstNI CAGNNNCTG 3 cut(s) 778, 802, 925
PsuI RGATCY 1 cut(s) 863
PvuII CAGCTG 2 cut(s) 775, 922
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
RseI CAYNNNNRTG 2 cut(s) 1079, 1085
SaqAI TTAA 5 cut(s) 181, 446, 478, 692, 763
SatI GCNGC 7 cut(s) 89, 557, 677, 776, 920, 923, 926
Sau3AI GATC 4 cut(s) 73, 455, 636, 863
Sau96I GGNCC 6 cut(s) 14, 79, 371, 417, 768, 1031
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 1 cut(s) 648
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 977
SfaNI GCATC 3 cut(s) 45, 311, 663
SfcI CTRYAG 2 cut(s) 45, 404
SfoI GGCGCC 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 4 cut(s) 14, 79, 417, 768
SmiMI CAYNNNNRTG 2 cut(s) 1079, 1085
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 7 cut(s) 391, 426, 479, 497, 693, 824, 845
SseBI AGGCCT 1 cut(s) 627
SsiI CCGC 2 cut(s) 557, 994
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 1 cut(s) 704
SspMI CTAG 5 cut(s) 238, 631, 659, 874, 902
StuI AGGCCT 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 3 cut(s) 187, 542, 1027
TaaI ACNGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 147, 252, 454
TasI AATT 7 cut(s) 391, 426, 479, 497, 693, 824, 845
TatI WGTACW 1 cut(s) 749
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 5 cut(s) 181, 446, 478, 692, 763
Tru9I TTAA 5 cut(s) 181, 446, 478, 692, 763
TscAI CASTG 2 cut(s) 57, 216
TseFI GTSAC 2 cut(s) 432, 1010
TseI GCWGC 6 cut(s) 88, 676, 775, 919, 922, 925
Tsp45I GTSAC 2 cut(s) 432, 1010
TspDTI ATGAA 2 cut(s) 326, 502
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 2 cut(s) 57, 216
Van91I CCANNNNNTGG 1 cut(s) 778
VpaK11BI GGWCC 4 cut(s) 14, 79, 417, 768
XapI RAATTY 1 cut(s) 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
XspI CTAG 5 cut(s) 238, 631, 659, 874, 902
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.