Rroxscaffold_2G00136530

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014828
Physical Location & Seq
Reverse (-)
74229297 .. 74230146
850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136530.1

Sequence Viewer

Length: 702 bp
ATGGAAACAAGAGCTGACCTCAAGGGAGTCAAATCCCAAGTCCAGAATCTGGTTGAAGAGTTACATGGCACTTGTGTAAATACTCAAAGAGAAGCAACACTTCAGCTCCGACTACTCGCAAAGCATAACATGAACAATCGGATTGTTATTGCAAACTGTGGAGCCATAAGCTTTTTGGTGGGTTTGATAAGCTCAACTGACAAATGGGTACAAGAAAATGCTGCAATTGAGTCTCTGGTTCATGTGCTTGAGAATGGGAATGATGAGGCCAAGGAGAACTCAGCTGCCATATTGTTTAGCCTTTCGATGATCTGGGAAAACAAGGTTCGGATTGGAAGGTCCAAGGCTATTAGGCCTCTAGTTGAGTCGTTACTAGGGAATGGGAGTCTTAGAGGGGGAAAAAGATGCAGCTACACTGGTGCTGTGAAGTACTTGGTGGAGTTTGTGATGAATCCAGAAGCTGGAATGGTTGACCAGGCCATTGCTGTTCTGGCTGCTTTTTGTACAAGTCATGAAGGGAGGATAGCAATTGTTGAGGAAGGTGGGGTTCCTGTTCTGGTGGAGGAGAATGCAGCTGCTGCTCTATTGCAGCTCTGCACAAATAGTGAAGAACAATGTAGTATGGTGGTTGATAAAGGAGTTGTTCCATCTTTGGTGGCATTGTCACAGTCTGGCACCCCAAGGGCCAAAGAAAAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

233

Amino Acids

25.07

Weight (kDa)

5.88

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 139 - 232 7.9e-06 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 2 cut(s) 49, 461
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 3 cut(s) 210, 431, 505
AfiI CCNNNNNNNGG 2 cut(s) 49, 461
AgsI TTSAA 1 cut(s) 56
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 9 cut(s) 14, 106, 171, 192, 284, 411, 461, 575, 592
AluI AGCT 9 cut(s) 14, 106, 171, 192, 284, 411, 461, 575, 592
Alw26I GTCTC 1 cut(s) 237
AlwNI CAGNNNCTG 3 cut(s) 49, 461, 578
AoxI GGCC 4 cut(s) 267, 353, 477, 684
ApeKI GCWGC 8 cut(s) 221, 284, 408, 494, 572, 575, 578, 589
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
AspS9I GGNCC 2 cut(s) 339, 684
AvaII GGWCC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 674
BbvI GCAGC 8 cut(s) 208, 271, 420, 481, 562, 565, 584, 601
BccI CCATC 1 cut(s) 655
BciT130I CCWGG 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 237
BfaI CTAG 2 cut(s) 359, 374
BisI GCNGC 8 cut(s) 222, 285, 409, 495, 573, 576, 579, 590
BlsI GCNGC 8 cut(s) 223, 286, 410, 496, 574, 577, 580, 591
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 476
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 339, 684
BmiI GGNNCC 3 cut(s) 163, 549, 676
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 395
BplI GAGNNNNNCTC 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 5, 269
BsaJI CCNNGG 3 cut(s) 270, 342, 680
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 461
Bse1I ACTGG 1 cut(s) 421
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 3 cut(s) 270, 342, 680
BseLI CCNNNNNNNGG 2 cut(s) 49, 461
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 8 cut(s) 208, 271, 420, 481, 562, 565, 584, 601
BsgI GTGCAG 1 cut(s) 580
BshFI GGCC 4 cut(s) 269, 355, 479, 686
BshNI GGYRCC 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 49, 461
BsmAI GTCTC 1 cut(s) 237
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 4 cut(s) 269, 355, 479, 686
Bsp1407I TGTACA 1 cut(s) 503
Bsp143I GATC 1 cut(s) 309
BspANI GGCC 4 cut(s) 269, 355, 479, 686
BspCNI CTCAG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 163, 549, 676
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 503
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 3 cut(s) 270, 342, 680
BssMI GATC 1 cut(s) 309
BssT1I CCWWGG 3 cut(s) 270, 342, 680
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 158, 669
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 503
BstDEI CTNAG 2 cut(s) 280, 389
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 491, 578
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 474
BstV1I GCAGC 8 cut(s) 208, 271, 420, 481, 562, 565, 584, 601
BsuRI GGCC 4 cut(s) 269, 355, 479, 686
BtsIMutI CAGTG 1 cut(s) 414
CaiI CAGNNNCTG 3 cut(s) 49, 461, 578
CciI TCATGA 1 cut(s) 511
Cfr13I GGNCC 2 cut(s) 339, 684
Csp6I GTAC 3 cut(s) 209, 430, 504
CviAII CATG 4 cut(s) 65, 130, 242, 512
CviQI GTAC 3 cut(s) 209, 430, 504
DdeI CTNAG 2 cut(s) 280, 389
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 3 cut(s) 270, 342, 680
Eco147I AGGCCT 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 3 cut(s) 270, 342, 680
ErhI CCWWGG 3 cut(s) 270, 342, 680
FaeI CATG 4 cut(s) 68, 133, 245, 515
FaiI YATR 9 cut(s) 66, 126, 131, 167, 243, 290, 513, 623, 700
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 4 cut(s) 64, 129, 241, 511
Fnu4HI GCNGC 8 cut(s) 222, 285, 409, 495, 573, 576, 579, 590
Fsp4HI GCNGC 8 cut(s) 222, 285, 409, 495, 573, 576, 579, 590
FspBI CTAG 2 cut(s) 359, 374
GluI GCNGC 8 cut(s) 222, 285, 409, 495, 573, 576, 579, 590
HaeIII GGCC 4 cut(s) 269, 355, 479, 686
Hin1II CATG 4 cut(s) 68, 133, 245, 515
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 6 cut(s) 27, 46, 230, 365, 385, 451
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 3 cut(s) 110, 141, 330
Hpy188III TCNNGA 3 cut(s) 43, 455, 512
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 3 cut(s) 330, 509, 533
HpyCH4III ACNGT 2 cut(s) 158, 669
HpyCH4V TGCA 6 cut(s) 152, 224, 408, 572, 589, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 578
HpyF3I CTNAG 2 cut(s) 280, 389
Hsp92II CATG 4 cut(s) 68, 133, 245, 515
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 2 cut(s) 111, 161
Lsp1109I GCAGC 8 cut(s) 208, 271, 420, 481, 562, 565, 584, 601
LweI GCATC 1 cut(s) 395
MaeI CTAG 2 cut(s) 359, 374
MaeIII GTNAC 3 cut(s) 60, 369, 663
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 2 cut(s) 68, 620
MfeI CAATTG 2 cut(s) 225, 528
MluCI AATT 2 cut(s) 225, 528
MlyI GAGTC 4 cut(s) 36, 239, 374, 394
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 29, 259, 366, 386, 513, 529, 556
MspA1I CMGCKG 2 cut(s) 284, 575
MspR9I CCNGG 1 cut(s) 476
MunI CAATTG 2 cut(s) 225, 528
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 491, 578
NdeII GATC 1 cut(s) 309
NlaIII CATG 4 cut(s) 68, 133, 245, 515
NlaIV GGNNCC 3 cut(s) 163, 549, 676
NmuCI GTSAC 1 cut(s) 663
PagI TCATGA 1 cut(s) 511
PceI AGGCCT 1 cut(s) 355
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 46, 451
PflMI CCANNNNNTGG 2 cut(s) 49, 461
PkrI GCNGC 8 cut(s) 223, 286, 410, 496, 574, 577, 580, 591
PleI GAGTC 4 cut(s) 35, 238, 373, 393
PpsI GAGTC 4 cut(s) 35, 238, 373, 393
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 3 cut(s) 163, 549, 676
PspPI GGNCC 2 cut(s) 339, 684
PstNI CAGNNNCTG 3 cut(s) 49, 461, 578
PvuII CAGCTG 2 cut(s) 284, 575
RsaI GTAC 3 cut(s) 210, 431, 505
RsaNI GTAC 3 cut(s) 209, 430, 504
SatI GCNGC 8 cut(s) 222, 285, 409, 495, 573, 576, 579, 590
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 2 cut(s) 339, 684
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 4 cut(s) 36, 239, 374, 394
ScrFI CCNGG 1 cut(s) 476
SfaNI GCATC 1 cut(s) 395
SinI GGWCC 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 20, 248
SmoI CTYRAG 2 cut(s) 20, 248
Sse9I AATT 2 cut(s) 225, 528
SseBI AGGCCT 1 cut(s) 355
SspMI CTAG 2 cut(s) 359, 374
StuI AGGCCT 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 3 cut(s) 270, 342, 680
TaaI ACNGT 2 cut(s) 158, 669
TaqI TCGA 1 cut(s) 305
TasI AATT 2 cut(s) 225, 528
TatI WGTACW 2 cut(s) 429, 503
TfiI GAWTC 2 cut(s) 46, 451
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 8 cut(s) 221, 284, 408, 494, 572, 575, 578, 589
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 4 cut(s) 146, 230, 464, 528
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 2 cut(s) 49, 461
VpaK11BI GGWCC 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 2 cut(s) 172, 487
XspI CTAG 2 cut(s) 359, 374
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.