RchiOBHm_Chr2g0146231

Nucleoside diphosphate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008671
Physical Location & Seq
Forward (+)
63925522 .. 63927735
2214 bp
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UTR
Exon/CDS
Intron
PRQ51600

Sequence Viewer

Length: 537 bp
ATGACGCTCCCCAGAGTTTCCTTCTTCTTCATCGTCGTCGTCGTCGTCTCTTTTTCTCTCATTTGCAGGTGTGATGGAATTACAGAGAAGGAGAAGACGTTAGCTATAATAAAGCCAGATGGGATGAGTGGTAACTACAGTGAAAAGATAAAGAATGCTATTTTGGATTCTGGGTTTACAATTCTCAAGGAAATGACCATTCAGCTTGATGAGGATGCTGCAATGGGATTCTACGCCGAGCATTCTTCGAGGAGCTTCTTTTCTAGCCTAGTCAAGTACATGACAAGTGGACCAGTATTGATTTTGGTTTTGGAGAAGGAAAATGCTGTTGCTGATTGGCGTGCTTTAATTGGCCCAACTGATGCGAGTGCAGCAAAGATTACTCATCCTCACAGCATCAGAGCAATGTGTGGGGTGACTATAGAAAAGAACTGTGTTCACGGGTCAGATTCTTTGCAATCAGCTCGACAAGAGATTGCATTCTTCTTTGAGGAGAAGTCTTCAGGTAGAGTAGTTACTGAGCATGACGAATTGTAG

Protein Analysis

178

Amino Acids

19.66

Weight (kDa)

5.54

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDK PF00334 31 - 166 1e-43 Nucleoside diphosphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 1 cut(s) 278
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 104, 205, 255, 464
AluI AGCT 4 cut(s) 104, 205, 255, 464
Alw26I GTCTC 1 cut(s) 52
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 2 cut(s) 218, 371
AspS9I GGNCC 2 cut(s) 290, 353
AsuHPI GGTGA 1 cut(s) 427
AvaII GGWCC 1 cut(s) 290
BbsI GAAGAC 2 cut(s) 101, 492
BbvI GCAGC 2 cut(s) 205, 383
BccI CCATC 2 cut(s) 68, 113
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 2 cut(s) 264, 269
BfmI CTRYAG 2 cut(s) 136, 420
BfuAI ACCTGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 219, 372
BlsI GCNGC 2 cut(s) 220, 373
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 2 cut(s) 290, 353
BmsI GCATC 3 cut(s) 205, 352, 405
BpiI GAAGAC 2 cut(s) 101, 492
BpuEI CTTGAG 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 2 cut(s) 228, 411
BseGI GGATG 3 cut(s) 129, 220, 385
BseMI GCAATG 2 cut(s) 228, 411
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 265, 506
BseXI GCAGC 2 cut(s) 205, 383
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 1 cut(s) 354
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmI GAATGC 3 cut(s) 160, 241, 479
BsnI GGCC 1 cut(s) 354
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 1 cut(s) 511
BspMI ACCTGC 1 cut(s) 57
BsrDI GCAATG 2 cut(s) 228, 411
BsrI ACTGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 140, 434
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 129, 220, 385
BstMAI GTCTC 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstSFI CTRYAG 2 cut(s) 136, 420
BstV1I GCAGC 2 cut(s) 205, 383
BstV2I GAAGAC 2 cut(s) 101, 492
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 3 cut(s) 129, 220, 385
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 342
Cfr13I GGNCC 2 cut(s) 290, 353
CseI GACGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 280, 524
CviJI RGCY 7 cut(s) 104, 115, 205, 255, 267, 354, 464
CviKI_1 RGCY 7 cut(s) 104, 115, 205, 255, 267, 354, 464
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 2 cut(s) 283, 527
FaiI YATR 4 cut(s) 107, 281, 422, 525
FatI CATG 2 cut(s) 279, 523
Fnu4HI GCNGC 2 cut(s) 219, 372
FokI GGATG 3 cut(s) 136, 227, 372
Fsp4HI GCNGC 2 cut(s) 219, 372
FspBI CTAG 2 cut(s) 264, 269
GluI GCNGC 2 cut(s) 219, 372
HaeIII GGCC 1 cut(s) 354
HgaI GACGC 1 cut(s) 13
Hin1II CATG 2 cut(s) 283, 527
HinfI GANTC 3 cut(s) 167, 228, 449
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 3 cut(s) 177, 290, 439
Hpy188I TCNGA 2 cut(s) 401, 448
Hpy8I GTNNAC 3 cut(s) 177, 290, 439
Hpy99I CGWCG 4 cut(s) 38, 41, 44, 47
HpyAV CCTTC 3 cut(s) 31, 82, 310
HpyCH4III ACNGT 2 cut(s) 140, 434
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 5 cut(s) 66, 221, 371, 457, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 2 cut(s) 283, 527
LmnI GCTCC 2 cut(s) 12, 252
LpnPI CCDG 6 cut(s) 25, 52, 129, 156, 306, 489
Lsp1109I GCAGC 2 cut(s) 205, 383
LweI GCATC 3 cut(s) 205, 352, 405
MaeI CTAG 2 cut(s) 264, 269
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 3 cut(s) 131, 415, 514
MboII GAAGA 6 cut(s) 16, 19, 106, 237, 475, 492
MluCI AATT 4 cut(s) 78, 180, 348, 530
MnlI CCTC 4 cut(s) 205, 243, 399, 484
MseI TTAA 1 cut(s) 347
Mva1269I GAATGC 3 cut(s) 160, 241, 479
MwoI GCNNNNNNNGC 1 cut(s) 371
NlaIII CATG 2 cut(s) 283, 527
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 415
PaqCI CACCTGC 1 cut(s) 57
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 42
PctI GAATGC 3 cut(s) 160, 241, 479
PfeI GAWTC 3 cut(s) 167, 228, 449
PkrI GCNGC 2 cut(s) 220, 373
PspPI GGNCC 2 cut(s) 290, 353
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 2 cut(s) 219, 372
Sau96I GGNCC 2 cut(s) 290, 353
SetI ASST 7 cut(s) 71, 101, 106, 207, 257, 466, 508
SfaNI GCATC 3 cut(s) 205, 352, 405
SfcI CTRYAG 2 cut(s) 136, 420
SinI GGWCC 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 4 cut(s) 78, 180, 348, 530
SspMI CTAG 2 cut(s) 264, 269
TaaI ACNGT 2 cut(s) 140, 434
TaiI ACGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 248, 466
TasI AATT 4 cut(s) 78, 180, 348, 530
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 3 cut(s) 167, 228, 449
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 2 cut(s) 218, 371
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 19
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 290
XspI CTAG 2 cut(s) 264, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.