Rh2BG462100

Nucleoside diphosphate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008671
Physical Location & Seq
Forward (+)
65372527 .. 65390726
18200 bp
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UTR
Exon/CDS
Intron
Rh2BG462100.1

Sequence Viewer

Length: 654 bp
ATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAATGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAAAAAAGAAGCTTAAGCTTCTTGGTTTCCCAAAAGATTCATTCTTGCACTTGACTTTGATTAATGGATTGTGTGATGGAATTACAGAGAAGGAGAAGACGTTAGCTATAATAAAGCCAGATGGGATGAGTGGTAACTACAGTGAAAAGATAAAGAATGCTATTTTGGATTCTGGGTTTACAATTCTCAAGGAAATGACCATTCAGGTTGATGAGGATGCTGCAATGGGATTCTACGCCGAGCATGCTTCGAGGAGCTTCTTTTCTAGCCTAGTCAAGTACATGACAAGTGGACCAGTATTGATTTTGGTTTTGGAGAAGGAAAATGCTGTTGCTGATTGGCGTGCTTTAATTGGCCCAACTGATGCGAGTGCAGCAAAGATTACTCATCCTCACAGCATCAGAGCAATGTGTGGGGTGACTATAGAAAAGAACTGTGTTCACGGGTCAGATTCTTTGCAATCAGCTCGACAAGAGATTGCATTCTTCTTTGAGGAGAAGTCTTCAGGTAGAGTAGTTACTGAGCATGACGAATTGTAG

Protein Analysis

217

Amino Acids

24.15

Weight (kDa)

6.44

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDK PF00334 70 - 205 2.6e-43 Nucleoside diphosphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 1 cut(s) 395
AflII CTTAAG 1 cut(s) 128
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 5 cut(s) 127, 133, 221, 372, 581
AluI AGCT 5 cut(s) 127, 133, 221, 372, 581
AlwI GGATC 1 cut(s) 36
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 2 cut(s) 335, 488
AseI ATTAAT 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 407, 470
AsuHPI GGTGA 1 cut(s) 544
AvaII GGWCC 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 218, 609
BbvI GCAGC 2 cut(s) 322, 500
BccI CCATC 2 cut(s) 185, 230
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BfaI CTAG 3 cut(s) 71, 381, 386
BfmI CTRYAG 2 cut(s) 253, 537
BfrI CTTAAG 1 cut(s) 128
BisI GCNGC 2 cut(s) 336, 489
BlsI GCNGC 2 cut(s) 337, 490
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 407, 470
BmsI GCATC 3 cut(s) 322, 469, 522
BpiI GAAGAC 2 cut(s) 218, 609
BpuEI CTTGAG 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 3 cut(s) 90, 345, 528
BseGI GGATG 3 cut(s) 246, 337, 502
BseMI GCAATG 3 cut(s) 90, 345, 528
BseMII CTCAG 2 cut(s) 38, 627
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 2 cut(s) 382, 623
BseXI GCAGC 2 cut(s) 322, 500
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 277, 596
BsnI GGCC 1 cut(s) 471
Bsp143I GATC 1 cut(s) 28
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 2 cut(s) 37, 628
BspPI GGATC 1 cut(s) 36
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 3 cut(s) 90, 345, 528
BsrI ACTGG 1 cut(s) 410
BssMI GATC 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 257, 551
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 360, 459
BstDEI CTNAG 2 cut(s) 24, 636
BstF5I GGATG 3 cut(s) 246, 337, 502
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 359, 488
BstNSI RCATGY 1 cut(s) 362
BstSFI CTRYAG 2 cut(s) 253, 537
BstV1I GCAGC 2 cut(s) 322, 500
BstV2I GAAGAC 2 cut(s) 218, 609
BsuRI GGCC 1 cut(s) 471
BtsCI GGATG 3 cut(s) 246, 337, 502
BtsIMutI CAGTG 1 cut(s) 262
Cac8I GCNNGC 2 cut(s) 360, 459
Cfr13I GGNCC 2 cut(s) 407, 470
Csp6I GTAC 1 cut(s) 394
CviAII CATG 5 cut(s) 34, 95, 359, 397, 641
CviJI RGCY 9 cut(s) 16, 127, 133, 221, 232, 372, 384, 471, 581
CviKI_1 RGCY 9 cut(s) 16, 127, 133, 221, 232, 372, 384, 471, 581
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 2 cut(s) 24, 636
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 1 cut(s) 603
FaeI CATG 5 cut(s) 37, 98, 362, 400, 644
FaiI YATR 9 cut(s) 20, 35, 51, 96, 224, 360, 398, 539, 642
FatI CATG 5 cut(s) 33, 94, 358, 396, 640
Fnu4HI GCNGC 2 cut(s) 336, 489
FokI GGATG 3 cut(s) 253, 344, 489
Fsp4HI GCNGC 2 cut(s) 336, 489
FspBI CTAG 3 cut(s) 71, 381, 386
GluI GCNGC 2 cut(s) 336, 489
HaeIII GGCC 1 cut(s) 471
Hin1II CATG 5 cut(s) 37, 98, 362, 400, 644
HindIII AAGCTT 2 cut(s) 125, 131
HinfI GANTC 5 cut(s) 88, 152, 284, 345, 566
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 3 cut(s) 294, 407, 556
Hpy188I TCNGA 2 cut(s) 518, 565
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 3 cut(s) 294, 407, 556
HpyAV CCTTC 2 cut(s) 199, 427
HpyCH4III ACNGT 2 cut(s) 257, 551
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 7 cut(s) 4, 115, 163, 338, 488, 574, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 488
HpyF3I CTNAG 2 cut(s) 24, 636
HpySE526I ACGT 1 cut(s) 215
Hsp92II CATG 5 cut(s) 37, 98, 362, 400, 644
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 1 cut(s) 369
LpnPI CCDG 6 cut(s) 11, 246, 273, 305, 423, 606
Lsp1109I GCAGC 2 cut(s) 322, 500
LweI GCATC 3 cut(s) 322, 469, 522
MaeI CTAG 3 cut(s) 71, 381, 386
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 3 cut(s) 248, 532, 631
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 3 cut(s) 223, 592, 609
MluCI AATT 4 cut(s) 195, 297, 465, 647
MnlI CCTC 4 cut(s) 322, 360, 516, 601
MseI TTAA 3 cut(s) 129, 177, 464
MspCI CTTAAG 1 cut(s) 128
Mva1269I GAATGC 2 cut(s) 277, 596
MwoI GCNNNNNNNGC 2 cut(s) 359, 488
NdeII GATC 1 cut(s) 28
NlaIII CATG 5 cut(s) 37, 98, 362, 400, 644
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 1 cut(s) 362
PaeI GCATGC 1 cut(s) 362
PctI GAATGC 2 cut(s) 277, 596
PfeI GAWTC 5 cut(s) 88, 152, 284, 345, 566
PkrI GCNGC 2 cut(s) 337, 490
PshBI ATTAAT 1 cut(s) 177
PspPI GGNCC 2 cut(s) 407, 470
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
SaqAI TTAA 3 cut(s) 129, 177, 464
SatI GCNGC 2 cut(s) 336, 489
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 2 cut(s) 407, 470
SetI ASST 8 cut(s) 129, 135, 218, 223, 324, 374, 583, 625
SfaNI GCATC 3 cut(s) 322, 469, 522
SfcI CTRYAG 2 cut(s) 253, 537
SinI GGWCC 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 128, 302
SmoI CTYRAG 2 cut(s) 128, 302
SphI GCATGC 1 cut(s) 362
Sse9I AATT 4 cut(s) 195, 297, 465, 647
SspMI CTAG 3 cut(s) 71, 381, 386
TaaI ACNGT 2 cut(s) 257, 551
TaiI ACGT 1 cut(s) 218
TaqI TCGA 2 cut(s) 365, 583
TasI AATT 4 cut(s) 195, 297, 465, 647
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 5 cut(s) 88, 152, 284, 345, 566
Tru1I TTAA 3 cut(s) 129, 177, 464
Tru9I TTAA 3 cut(s) 129, 177, 464
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 2 cut(s) 335, 488
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 3 cut(s) 50, 101, 144
TspRI CASTG 1 cut(s) 262
Vha464I CTTAAG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 407
VspI ATTAAT 1 cut(s) 177
XceI RCATGY 1 cut(s) 362
XspI CTAG 3 cut(s) 71, 381, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.