Rh2AG449900

Nucleoside diphosphate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008671
Physical Location & Seq
Forward (+)
66334271 .. 66343602
9332 bp
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UTR
Exon/CDS
Intron
Rh2AG449900.1

Sequence Viewer

Length: 480 bp
ATGTCTCGTGACCGGCTCTTCTGCTGCGTCATGACGCTCCCCAGAGTTTCCTTCTTCTTCATCGTCGTGGTCGTCGTCTCTGTTTCTCTCATTTGCAGGTGTGATGGAATTACAGAGAAGGAGAAGACGTTAGCTATAATAAAGCCAGATGGGATGAGTGGTAACTACAGTGAAAAGATAAAGAATGCTATTTTGGATTCTGGGTTTACAATTCTCAAGGAAATGACCATTCAGCTTGATGAGGATGCTGCAATGGGATTCTACGCCGAGCATGCTTCGAGGAGCTTCTTTTCTAGCCTAGTCAAGTACATGACAAGTGGACCAGTATTGATTATGGTTTTGGAGAAGGAAAATGCTGTTGCTGATTGGCGTGCTTTAATTGGCCCAACTGATGCGAGTGCAGCAAAGATTACTCATCCTCACAGGTCTGTTTTGCTCTTCTTTCAAAATGTCTTATACGATAATTTCAGCTGCTATTAA

Protein Analysis

159

Amino Acids

17.81

Weight (kDa)

6.28

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDK PF00334 41 - 136 2e-29 Nucleoside diphosphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 87
AfaI GTAC 1 cut(s) 308
AgsI TTSAA 1 cut(s) 446
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 4 cut(s) 134, 235, 285, 471
AluI AGCT 4 cut(s) 134, 235, 285, 471
Alw26I GTCTC 2 cut(s) 9, 82
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 4 cut(s) 24, 248, 401, 471
AspS9I GGNCC 2 cut(s) 320, 383
AvaII GGWCC 1 cut(s) 320
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 4 cut(s) 11, 235, 413, 458
BccI CCATC 2 cut(s) 98, 143
BcoDI GTCTC 2 cut(s) 9, 82
BfaI CTAG 2 cut(s) 294, 299
BfmI CTRYAG 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 87
BisI GCNGC 4 cut(s) 25, 249, 402, 472
BlsI GCNGC 4 cut(s) 26, 250, 403, 473
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 320, 383
BmsI GCATC 2 cut(s) 235, 382
BpiI GAAGAC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 258
BseGI GGATG 3 cut(s) 159, 250, 415
BseMI GCAATG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 323
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 4 cut(s) 11, 235, 413, 458
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 13
BsmAI GTCTC 2 cut(s) 9, 82
BsmBI CGTCTC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 1 cut(s) 384
BspANI GGCC 1 cut(s) 384
BspHI TCATGA 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 87
BspQI GCTCTTC 2 cut(s) 23, 443
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 1 cut(s) 323
BssAI RCCGGY 1 cut(s) 12
BssSI CACGAG 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 2 cut(s) 23, 443
BstC8I GCNNGC 2 cut(s) 273, 372
BstF5I GGATG 3 cut(s) 159, 250, 415
BstMAI GTCTC 2 cut(s) 9, 82
BstMWI GCNNNNNNNGC 2 cut(s) 272, 401
BstNSI RCATGY 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 4 cut(s) 11, 235, 413, 458
BstV2I GAAGAC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 3 cut(s) 159, 250, 415
BtsIMutI CAGTG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 87
Cac8I GCNNGC 2 cut(s) 273, 372
CciI TCATGA 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 2 cut(s) 320, 383
CseI GACGC 2 cut(s) 16, 43
Csp6I GTAC 1 cut(s) 307
CviAII CATG 3 cut(s) 31, 272, 310
CviJI RGCY 8 cut(s) 16, 134, 145, 235, 285, 297, 384, 471
CviKI_1 RGCY 8 cut(s) 16, 134, 145, 235, 285, 297, 384, 471
CviQI GTAC 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 23, 443
EarI CTCTTC 2 cut(s) 23, 443
Eco47I GGWCC 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 34, 275, 313
FaiI YATR 6 cut(s) 32, 137, 273, 311, 335, 457
FatI CATG 3 cut(s) 30, 271, 309
Fnu4HI GCNGC 4 cut(s) 25, 249, 402, 472
FokI GGATG 3 cut(s) 166, 257, 402
Fsp4HI GCNGC 4 cut(s) 25, 249, 402, 472
FspBI CTAG 2 cut(s) 294, 299
GluI GCNGC 4 cut(s) 25, 249, 402, 472
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 13
HgaI GACGC 2 cut(s) 16, 43
Hin1II CATG 3 cut(s) 34, 275, 313
HinfI GANTC 2 cut(s) 197, 258
HpaII CCGG 1 cut(s) 13
Hpy166II GTNNAC 2 cut(s) 207, 320
Hpy188III TCNNGA 2 cut(s) 8, 31
Hpy8I GTNNAC 2 cut(s) 207, 320
Hpy99I CGWCG 2 cut(s) 68, 77
HpyAV CCTTC 3 cut(s) 61, 112, 340
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 3 cut(s) 96, 251, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 401
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 3 cut(s) 34, 275, 313
LguI GCTCTTC 2 cut(s) 23, 443
LmnI GCTCC 2 cut(s) 42, 282
LpnPI CCDG 7 cut(s) 26, 55, 82, 159, 186, 336, 409
Lsp1109I GCAGC 4 cut(s) 11, 235, 413, 458
LweI GCATC 2 cut(s) 235, 382
MaeI CTAG 2 cut(s) 294, 299
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 8, 161
MboII GAAGA 5 cut(s) 10, 46, 49, 136, 430
MluCI AATT 4 cut(s) 108, 210, 378, 463
MnlI CCTC 3 cut(s) 235, 273, 429
MseI TTAA 2 cut(s) 377, 478
MslI CAYNNNNRTG 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 471
MspI CCGG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 272, 401
NlaIII CATG 3 cut(s) 34, 275, 313
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 1 cut(s) 275
PaeI GCATGC 1 cut(s) 275
PagI TCATGA 1 cut(s) 30
PaqCI CACCTGC 1 cut(s) 87
PciSI GCTCTTC 2 cut(s) 23, 443
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 72
PctI GAATGC 1 cut(s) 190
PfeI GAWTC 2 cut(s) 197, 258
PkrI GCNGC 4 cut(s) 26, 250, 403, 473
PspPI GGNCC 2 cut(s) 320, 383
PvuII CAGCTG 1 cut(s) 471
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 65
SapI GCTCTTC 2 cut(s) 23, 443
SaqAI TTAA 2 cut(s) 377, 478
SatI GCNGC 4 cut(s) 25, 249, 402, 472
Sau96I GGNCC 2 cut(s) 320, 383
SetI ASST 7 cut(s) 101, 131, 136, 237, 287, 428, 473
SfaNI GCATC 2 cut(s) 235, 382
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SphI GCATGC 1 cut(s) 275
Sse9I AATT 4 cut(s) 108, 210, 378, 463
SspMI CTAG 2 cut(s) 294, 299
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 278
TasI AATT 4 cut(s) 108, 210, 378, 463
TatI WGTACW 1 cut(s) 306
TfiI GAWTC 2 cut(s) 197, 258
Tru1I TTAA 2 cut(s) 377, 478
Tru9I TTAA 2 cut(s) 377, 478
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 4 cut(s) 24, 248, 401, 471
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 49
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 320
XceI RCATGY 1 cut(s) 275
XspI CTAG 2 cut(s) 294, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.