RchiOBHm_Chr4g0432801

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005369
Physical Location & Seq
Forward (+)
56860424 .. 56861433
1010 bp
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UTR
Exon/CDS
Intron
PRQ40137

Sequence Viewer

Length: 552 bp
ATGATCTCAGCATATGGTGGCTCGCAGATTTTTATGGAGCAAAATATCGGTCAATTGGCCATAAAGTATCCAAAATTCAGTTCCCTGCTATGGGGTCACTTTTGTAATTGGATAACCAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACTTTATTGACTGCAACATCTGTATTTATTATTGCTTTCATTGCTGCACCTCCGGTAGATATTGATGGTATTCGTGAACCTGTTTCTGGATCTTTACTTTACGGAAACAATATTATTTCTAGTGCCATTATCCCTACTTTTGCAGCTATAGGTTTGCACTTTTACCCAATATGGAAAGCAGCTTCCGTTGATGAATGGTTATACAACGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTACTATATGGGTCGTGAGTGGGAACTTATTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATCTACGTGCTTACTACTATTGCGATGGGGTTTGTTGTGTTTGGAATTCCTGCTTGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Photo_RC

Protein Analysis

183

Amino Acids

20.64

Weight (kDa)

5.87

Isoelectric Point (pI)

31.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 45 - 165 1.5e-30 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 157, 265
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 2 cut(s) 74, 533
AfiI CCNNNNNNNGG 3 cut(s) 90, 91, 254
AgsI TTSAA 1 cut(s) 545
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AloI GAACNNNNNNTCC 2 cut(s) 438, 470
AluBI AGCT 4 cut(s) 314, 350, 391, 422
AluI AGCT 4 cut(s) 314, 350, 391, 422
AlwI GGATC 2 cut(s) 157, 265
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 212, 311, 347
ApoI RAATTY 2 cut(s) 74, 533
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 1 cut(s) 380
AvaII GGWCC 1 cut(s) 380
BalI TGGCCA 1 cut(s) 59
BbvI GCAGC 3 cut(s) 199, 323, 359
BccI CCATC 3 cut(s) 138, 227, 507
BciVI GTATCC 1 cut(s) 78
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 315
BfuI GTATCC 1 cut(s) 78
BisI GCNGC 3 cut(s) 213, 312, 348
BlsI GCNGC 3 cut(s) 214, 313, 349
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 380
BmsI GCATC 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 473
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 91, 254
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 473
BseGI GGATG 1 cut(s) 149
BseLI CCNNNNNNNGG 3 cut(s) 90, 91, 254
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 199, 323, 359
BsgI GTGCAG 1 cut(s) 198
BshFI GGCC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 90, 91, 254
BsnI GGCC 1 cut(s) 59
Bsp143I GATC 3 cut(s) 3, 162, 257
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 1 cut(s) 20
BspPI GGATC 2 cut(s) 157, 265
BsrDI GCAATG 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 473
BssMI GATC 3 cut(s) 3, 162, 257
BssT1I CCWWGG 1 cut(s) 473
Bst4CI ACNGT 2 cut(s) 131, 377
BstBAI YACGTR 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 1 cut(s) 7
BstF5I GGATG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 3 cut(s) 6, 165, 260
BstMBI GATC 3 cut(s) 3, 162, 257
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 199, 323, 359
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BsuI GTATCC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 59
BtgZI GCGATG 1 cut(s) 526
BtsCI GGATG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 23
CfoI GCGC 1 cut(s) 471
Cfr13I GGNCC 1 cut(s) 380
CviJI RGCY 6 cut(s) 21, 59, 314, 350, 391, 422
CviKI_1 RGCY 6 cut(s) 21, 59, 314, 350, 391, 422
DdeI CTNAG 1 cut(s) 7
DpnI GATC 3 cut(s) 5, 164, 259
DpnII GATC 3 cut(s) 3, 162, 257
EaeI YGGCCR 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 473
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 213, 312, 348
FokI GGATG 1 cut(s) 156
Fsp4HI GCNGC 3 cut(s) 213, 312, 348
FspBI CTAG 1 cut(s) 288
GlaI GCGC 1 cut(s) 470
GluI GCNGC 3 cut(s) 213, 312, 348
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 221
HhaI GCGC 1 cut(s) 471
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HpaII CCGG 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 242, 255, 437
Hpy8I GTNNAC 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 131, 377
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 5 cut(s) 182, 215, 311, 325, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 7
HpySE526I ACGT 1 cut(s) 494
HspAI GCGC 1 cut(s) 469
Kzo9I GATC 3 cut(s) 3, 162, 257
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 6 cut(s) 98, 130, 234, 240, 261, 446
Lsp1109I GCAGC 3 cut(s) 199, 323, 359
LweI GCATC 1 cut(s) 497
MaeI CTAG 1 cut(s) 288
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 5, 164, 259
MboI GATC 3 cut(s) 3, 162, 257
MfeI CAATTG 1 cut(s) 53
MflI RGATCY 1 cut(s) 257
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 5 cut(s) 53, 74, 106, 393, 533
MluNI TGGCCA 1 cut(s) 59
MnlI CCTC 1 cut(s) 228
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 1 cut(s) 221
MunI CAATTG 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeI CATATG 1 cut(s) 13
NdeII GATC 3 cut(s) 3, 162, 257
NmuCI GTSAC 1 cut(s) 95
PkrI GCNGC 3 cut(s) 214, 313, 349
Ppu21I YACGTR 1 cut(s) 495
PspPI GGNCC 1 cut(s) 380
PsuI RGATCY 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 493
SatI GCNGC 3 cut(s) 213, 312, 348
Sau3AI GATC 3 cut(s) 3, 162, 257
Sau96I GGNCC 1 cut(s) 380
SetI ASST 8 cut(s) 220, 250, 316, 322, 352, 393, 424, 497
SfaNI GCATC 1 cut(s) 497
SfcI CTRYAG 1 cut(s) 315
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 493
Sse9I AATT 5 cut(s) 53, 74, 106, 393, 533
SspI AATATT 1 cut(s) 280
SspMI CTAG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 2 cut(s) 131, 377
TaiI ACGT 1 cut(s) 497
TaqII GACCGA 1 cut(s) 38
TasI AATT 5 cut(s) 53, 74, 106, 393, 533
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 212, 311, 347
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 196, 375
TspGWI ACGGA 3 cut(s) 285, 343, 446
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 2 cut(s) 74, 533
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.