Rh1AG254400

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005369
Physical Location & Seq
Reverse (-)
47044469 .. 47044995
527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG254400.1

Sequence Viewer

Length: 393 bp
ATGGAGAAGACAAAATTGTTTCAAGCACCTACAGAAGTGCCCCTTGTTTCAAAGAGAGGAGGACGGGTTGTAGCTTGCTACATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCACCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTTTCTGACGGTATGCCTCTAGGAATCTTTGGTACTTTCAACTTCATGATTGTATTCCAAGCTGAGCACAACATCCTTATGCACCCATTTCACATGCTAGGTGTGGCTGGTGTATTCGGCGGCTCCTGGAAATTCCACCCAGAAGGTAATGGGTTACTCCTACAGCACGTCCTTGGACAATACTCAAGGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Photo_RC

Protein Analysis

130

Amino Acids

14.41

Weight (kDa)

8.89

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 24 - 111 5.4e-35 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 318
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 1 cut(s) 232
AgsI TTSAA 3 cut(s) 23, 51, 238
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 1 cut(s) 323
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 5 cut(s) 74, 149, 161, 198, 260
AluI AGCT 5 cut(s) 74, 149, 161, 198, 260
Alw21I GWGCWC 1 cut(s) 267
AlwNI CAGNNNCTG 2 cut(s) 152, 164
ApeKI GCWGC 2 cut(s) 158, 161
ApoI RAATTY 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 148, 170
BciT130I CCWGG 1 cut(s) 325
BfaI CTAG 3 cut(s) 218, 296, 391
BfmI CTRYAG 2 cut(s) 30, 359
BisI GCNGC 3 cut(s) 159, 162, 319
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 3 cut(s) 160, 163, 320
Bme1390I CCNGG 1 cut(s) 325
BmgBI CACGTC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 125, 370
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 125, 370
BseGI GGATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 2 cut(s) 148, 170
BsiHKAI GWGCWC 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 42, 267
Bsp143I GATC 1 cut(s) 174
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 125, 370
BssMI GATC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 125, 370
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 101, 261
BstF5I GGATG 1 cut(s) 270
BstKTI GATC 1 cut(s) 177
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 146, 155
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 2 cut(s) 30, 359
BstSLI GKGCMC 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 148, 170
BstV2I GAAGAC 1 cut(s) 14
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 2 cut(s) 152, 164
CciI TCATGA 1 cut(s) 243
Csp6I GTAC 1 cut(s) 231
CviAII CATG 3 cut(s) 82, 244, 292
CviJI RGCY 8 cut(s) 74, 149, 161, 198, 260, 305, 321, 388
CviKI_1 RGCY 8 cut(s) 74, 149, 161, 198, 260, 305, 321, 388
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 2 cut(s) 101, 261
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 125, 370
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 125, 370
ErhI CCWWGG 2 cut(s) 125, 370
FaeI CATG 3 cut(s) 85, 247, 295
FaiI YATR 7 cut(s) 83, 119, 142, 212, 245, 278, 293
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FatI CATG 3 cut(s) 81, 243, 291
Fnu4HI GCNGC 3 cut(s) 159, 162, 319
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 3 cut(s) 159, 162, 319
FspBI CTAG 3 cut(s) 218, 296, 391
GluI GCNGC 3 cut(s) 159, 162, 319
Hin1II CATG 3 cut(s) 85, 247, 295
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 1 cut(s) 222
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 3 cut(s) 89, 172, 244
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 121, 140, 158, 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 155
HpyF3I CTNAG 2 cut(s) 101, 261
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 85, 247, 295
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 154, 326
LpnPI CCDG 6 cut(s) 98, 165, 291, 310, 337, 351
Lsp1109I GCAGC 2 cut(s) 148, 170
MaeI CTAG 3 cut(s) 218, 296, 391
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 351
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 1 cut(s) 19
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 42, 267
MluCI AATT 3 cut(s) 14, 183, 329
MnlI CCTC 3 cut(s) 50, 53, 225
MroXI GAANNNNTTC 1 cut(s) 199
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 325
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 146, 155
NdeII GATC 1 cut(s) 174
NlaIII CATG 3 cut(s) 85, 247, 295
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 1 cut(s) 295
PagI TCATGA 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 323
PkrI GCNGC 3 cut(s) 160, 163, 320
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 322
PstNI CAGNNNCTG 2 cut(s) 152, 164
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 275
SatI GCNGC 3 cut(s) 159, 162, 319
Sau3AI GATC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 2 cut(s) 42, 267
SetI ASST 9 cut(s) 31, 76, 151, 163, 200, 262, 301, 346, 369
SfcI CTRYAG 2 cut(s) 30, 359
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 3 cut(s) 14, 183, 329
SsiI CCGC 1 cut(s) 318
SspMI CTAG 3 cut(s) 218, 296, 391
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 2 cut(s) 125, 370
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 1 cut(s) 369
TasI AATT 3 cut(s) 14, 183, 329
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 1 cut(s) 222
TseI GCWGC 2 cut(s) 158, 161
TspDTI ATGAA 1 cut(s) 232
TspGWI ACGGA 1 cut(s) 98
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 3 cut(s) 218, 296, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.