Rroxscaffold_7G00179730

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005369
Physical Location & Seq
Reverse (-)
19231180 .. 19231868
689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179730.1

Sequence Viewer

Length: 528 bp
ATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTTTCTGACGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATGATTGCATTCCAAGCTGAGCACAACATCCTTATGCACCCATTTCACATGCTAGGTGTGGCTGGTGTATTCGGCGGATCCCTATTCAGTGCTATGCATGGTTCTTTGGTAACCTCTAGTTTGATTAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTTGGTGAAGAGGAAGAAACTTATAATATCGTAGCCACTCATGGTATTTTTGGCCGATTGATCTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACACTTCTTCTTAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACCGCTTTAGGTATCAGCACTATGGCTTTCAACTTAAATATATGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Photo_RC

Protein Analysis

175

Amino Acids

19.34

Weight (kDa)

5.59

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 1 - 168 3.2e-60 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AciI CCGC 2 cut(s) 243, 480
AclWI GGATC 2 cut(s) 240, 253
AcoI YGGCCR 1 cut(s) 388
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 163, 418, 508
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 5 cut(s) 68, 80, 123, 185, 449
AluI AGCT 5 cut(s) 68, 80, 123, 185, 449
Alw21I GWGCWC 1 cut(s) 192
AlwI GGATC 2 cut(s) 240, 253
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 2 cut(s) 388, 455
ApeKI GCWGC 3 cut(s) 77, 80, 449
Asp700I GAANNNNTTC 1 cut(s) 124
AspLEI GCGC 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 353, 469
BamHI GGATCC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 67, 89, 436
BfaI CTAG 4 cut(s) 143, 221, 285, 411
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 3 cut(s) 78, 81, 450
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 3 cut(s) 79, 82, 451
BmiI GGNNCC 1 cut(s) 247
Bpu1102I GCTNAGC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMII CTCAG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 67, 89, 436
BshFI GGCC 2 cut(s) 390, 457
BsiHKAI GWGCWC 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 464
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 2 cut(s) 390, 457
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 99, 245, 396
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 2 cut(s) 243, 480
BspANI GGCC 2 cut(s) 390, 457
BspCNI CTCAG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 2 cut(s) 240, 253
BssECI CCNNGG 1 cut(s) 44
BssMI GATC 3 cut(s) 99, 245, 396
BssT1I CCWWGG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 1 cut(s) 339
BstDEI CTNAG 3 cut(s) 20, 186, 445
BstEII GGTNACC 1 cut(s) 277
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 3 cut(s) 102, 248, 399
BstMBI GATC 3 cut(s) 99, 245, 396
BstMWI GCNNNNNNNGC 4 cut(s) 65, 74, 86, 182
BstNSI RCATGY 1 cut(s) 220
BstPI GGTNACC 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 3 cut(s) 67, 89, 436
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 2 cut(s) 390, 457
BtsCI GGATG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 262
CaiI CAGNNNCTG 1 cut(s) 71
CciI TCATGA 1 cut(s) 168
CfoI GCGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 156
CviAII CATG 4 cut(s) 169, 217, 266, 377
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 3 cut(s) 20, 186, 445
DpnI GATC 3 cut(s) 101, 247, 398
DpnII GATC 3 cut(s) 99, 245, 396
EaeI YGGCCR 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 277
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoO65I GGTNACC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 4 cut(s) 172, 220, 269, 380
FatI CATG 4 cut(s) 168, 216, 265, 376
Fnu4HI GCNGC 3 cut(s) 78, 81, 450
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 3 cut(s) 78, 81, 450
FspBI CTAG 4 cut(s) 143, 221, 285, 411
GlaI GCGC 1 cut(s) 41
GluI GCNGC 3 cut(s) 78, 81, 450
HaeIII GGCC 2 cut(s) 390, 457
HhaI GCGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 172, 220, 269, 380
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 2 cut(s) 147, 314
HphI GGTGA 2 cut(s) 353, 469
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 8, 97, 169
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4V TGCA 5 cut(s) 59, 77, 176, 205, 265
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 74, 86, 182
HpyF3I CTNAG 3 cut(s) 20, 186, 445
Hsp92II CATG 4 cut(s) 172, 220, 269, 380
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 3 cut(s) 99, 245, 396
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 6 cut(s) 17, 84, 138, 216, 457, 471
Lsp1109I GCAGC 3 cut(s) 67, 89, 436
MaeI CTAG 4 cut(s) 143, 221, 285, 411
MaeIII GTNAC 2 cut(s) 277, 329
MalI GATC 3 cut(s) 101, 247, 398
MboI GATC 3 cut(s) 99, 245, 396
MboII GAAGA 4 cut(s) 356, 362, 391, 433
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 108, 421
MnlI CCTC 3 cut(s) 150, 292, 340
Mph1103I ATGCAT 1 cut(s) 267
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 2 cut(s) 512, 526
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 80
MunI CAATTG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 176
MwoI GCNNNNNNNGC 4 cut(s) 65, 74, 86, 182
NdeII GATC 3 cut(s) 99, 245, 396
NlaIII CATG 4 cut(s) 172, 220, 269, 380
NlaIV GGNNCC 1 cut(s) 247
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 220
PagI TCATGA 1 cut(s) 168
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 2 cut(s) 147, 314
PkrI GCNGC 3 cut(s) 79, 82, 451
PsiI TTATAA 1 cut(s) 360
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 2 cut(s) 512, 526
SatI GCNGC 3 cut(s) 78, 81, 450
Sau3AI GATC 3 cut(s) 99, 245, 396
SduI GDGCHC 1 cut(s) 192
SfcI CTRYAG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 200
Sse9I AATT 2 cut(s) 108, 421
SsiI CCGC 2 cut(s) 243, 480
SspMI CTAG 4 cut(s) 143, 221, 285, 411
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 134
TasI AATT 2 cut(s) 108, 421
TfiI GAWTC 2 cut(s) 147, 314
Tru1I TTAA 2 cut(s) 512, 526
Tru9I TTAA 2 cut(s) 512, 526
TscAI CASTG 1 cut(s) 262
TseI GCWGC 3 cut(s) 77, 80, 449
TspDTI ATGAA 3 cut(s) 157, 327, 339
TspGWI ACGGA 1 cut(s) 17
TspRI CASTG 1 cut(s) 262
XagI CCTNNNNNAGG 1 cut(s) 462
XceI RCATGY 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 4 cut(s) 143, 221, 285, 411
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.