Rmu_sc0017755.1_g000008

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017755.1
OG0005369
Physical Location & Seq
Forward (+)
28135 .. 28683
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017755.1_g000008.1.cds

Sequence Viewer

Length: 549 bp
atgcctctaggaatctctggtactttcaacttcatgattgtattccaagctgagcacaacatccttatgcacccatttcacatgctaggtgtggctcgtgtattcggcggctccctattcagtgctatgcatggttctttggtaacctctagtttgatcagcgaaaccacagaaaatgaatctgctaatgaaggttacagattcggtcaagaggaagaaacttataatatcgtagccgctcatggttattttggccgattgatcttccaatatgctagtttcaacaattctcgttctttacacttcttcttagctgcttggcctgtagtaggtatctggttcaccgctttaggtatcagcactatggctttcaacttaaatggatttaatttcaatcaatatgtagttgatagtcaaggtcgtgtaattaatacttgggctgatattattaaccgcgctaaccttggtatggaagttatgcatgaacgtaatgctcataatttccctctagacctagctgctgttgaagttccatctatcaatggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Photo_RC

Protein Analysis

182

Amino Acids

20.27

Weight (kDa)

5.5

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 239
AccII CGCG 1 cut(s) 456
AciI CCGC 4 cut(s) 108, 237, 345, 454
AcoI YGGCCR 1 cut(s) 253
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 329, 469
AgsI TTSAA 5 cut(s) 28, 283, 373, 394, 527
AluBI AGCT 3 cut(s) 50, 314, 518
AluI AGCT 3 cut(s) 50, 314, 518
Alw21I GWGCWC 1 cut(s) 57
AoxI GGCC 2 cut(s) 253, 320
ApeKI GCWGC 2 cut(s) 314, 518
AseI ATTAAT 1 cut(s) 429
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 334
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 301, 505
BccI CCATC 1 cut(s) 541
BclI TGATCA 1 cut(s) 156
BfaI CTAG 6 cut(s) 8, 86, 150, 276, 509, 515
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 4 cut(s) 109, 237, 315, 519
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 4 cut(s) 110, 238, 316, 520
BmiI GGNNCC 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 463
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 469
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 329, 469
BseMII CTCAG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 301, 505
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 255, 322
BsiHKAI GWGCWC 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 329, 469
BsnI GGCC 2 cut(s) 255, 322
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 156, 261
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 4 cut(s) 108, 237, 345, 454
BspANI GGCC 2 cut(s) 255, 322
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 2 cut(s) 156, 261
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 463
Bst2BI CACGAG 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 51, 310
BstEII GGTNACC 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 2 cut(s) 159, 264
BstMBI GATC 2 cut(s) 156, 261
BstNSI RCATGY 1 cut(s) 85
BstPI GGTNACC 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 301, 505
BsuRI GGCC 2 cut(s) 255, 322
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 127
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 21
CviAII CATG 5 cut(s) 34, 82, 131, 242, 482
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 2 cut(s) 51, 310
DpnI GATC 2 cut(s) 158, 263
DpnII GATC 2 cut(s) 156, 261
EaeI YGGCCR 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 463
Eco91I GGTNACC 1 cut(s) 142
EcoNI CCTNNNNNAGG 1 cut(s) 327
EcoO65I GGTNACC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 463
EcoT22I ATGCAT 2 cut(s) 132, 483
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 5 cut(s) 37, 85, 134, 245, 485
FatI CATG 5 cut(s) 33, 81, 130, 241, 481
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 4 cut(s) 109, 237, 315, 519
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 4 cut(s) 109, 237, 315, 519
FspBI CTAG 6 cut(s) 8, 86, 150, 276, 509, 515
GlaI GCGC 1 cut(s) 457
GluI GCNGC 4 cut(s) 109, 237, 315, 519
HaeIII GGCC 2 cut(s) 255, 322
HhaI GCGC 1 cut(s) 458
Hin1II CATG 5 cut(s) 37, 85, 134, 245, 485
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 3 cut(s) 12, 179, 201
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 342
Hpy188III TCNNGA 3 cut(s) 34, 209, 509
Hpy8I GTNNAC 1 cut(s) 342
HpyAV CCTTC 1 cut(s) 185
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 3 cut(s) 70, 130, 481
HpyF3I CTNAG 2 cut(s) 51, 310
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 5 cut(s) 37, 85, 134, 245, 485
HspAI GCGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 2 cut(s) 156, 261
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 3 cut(s) 3, 322, 336
Lsp1109I GCAGC 2 cut(s) 301, 505
MaeI CTAG 6 cut(s) 8, 86, 150, 276, 509, 515
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 142, 194
MalI GATC 2 cut(s) 158, 263
MbiI CCGCTC 1 cut(s) 239
MboI GATC 2 cut(s) 156, 261
MboII GAAGA 3 cut(s) 227, 256, 298
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 286, 388, 426, 499
MnlI CCTC 4 cut(s) 15, 157, 205, 516
Mph1103I ATGCAT 2 cut(s) 132, 483
MseI TTAA 4 cut(s) 377, 387, 429, 450
MslI CAYNNNNRTG 1 cut(s) 65
MvnI CGCG 1 cut(s) 456
NdeII GATC 2 cut(s) 156, 261
NlaIII CATG 5 cut(s) 37, 85, 134, 245, 485
NlaIV GGNNCC 1 cut(s) 112
NsiI ATGCAT 2 cut(s) 132, 483
NspI RCATGY 1 cut(s) 85
PagI TCATGA 1 cut(s) 33
PfeI GAWTC 3 cut(s) 12, 179, 201
PkrI GCNGC 4 cut(s) 110, 238, 316, 520
PshBI ATTAAT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 112
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 4 cut(s) 377, 387, 429, 450
SatI GCNGC 4 cut(s) 109, 237, 315, 519
Sau3AI GATC 2 cut(s) 156, 261
SduI GDGCHC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 4 cut(s) 286, 388, 426, 499
SsiI CCGC 4 cut(s) 108, 237, 345, 454
SspMI CTAG 6 cut(s) 8, 86, 150, 276, 509, 515
StyI CCWWGG 1 cut(s) 463
TaiI ACGT 1 cut(s) 490
TaqII GACCGA 1 cut(s) 194
TasI AATT 4 cut(s) 286, 388, 426, 499
TauI GCSGC 2 cut(s) 111, 239
TfiI GAWTC 3 cut(s) 12, 179, 201
Tru1I TTAA 4 cut(s) 377, 387, 429, 450
Tru9I TTAA 4 cut(s) 377, 387, 429, 450
TscAI CASTG 1 cut(s) 127
TseI GCWGC 2 cut(s) 314, 518
TspDTI ATGAA 4 cut(s) 22, 192, 204, 498
TspRI CASTG 1 cut(s) 127
VspI ATTAAT 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 327
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 85
XspI CTAG 6 cut(s) 8, 86, 150, 276, 509, 515
Zsp2I ATGCAT 2 cut(s) 132, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.