RchiOBHm_Chr4g0447061
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011558
Physical Location & Seq
Forward (+)
66948013 .. 66949093
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41454

Sequence Viewer

Length: 1080 bp
ATGGATCCGAAGGTCCTTACTTACGAAAGTGTTGTGCTGAGAATGAGTGACTTGGCACTGCTTAGAGGCCCACATTACTTGAACGATTGCATAATTGAATTCTATTTCAATTATCTTTCTTCCCTTTGTGATGATGACATCCTTCTGGTTTCACCTACAGTCTCATTTTGGCTGGCAAATTCTCAGAATTCGGAGGCTAATTTTAGAGACTTCGAGGAGTCTAATCAACTTGGTGAAAAGCAAGTTGTGATATTCACGGTGAATGATAATCAAGACATGAGCCAAAATGATGGCGGAAACCATTGGAGTTTGCTGGTGTATTACAGAAAATCCAATGCATTTGTGCATTACGACAGCTTGGGGGGTTTGAATAGCTTGGTTGCCAGGAAGTTTTACGGAGCAGTTAAGAAATATGTGGCAGCTGCAGCAACACAAACACCAACGTTTGGGACTAGTAGTTTGGTTGCCACCACTAGGTCTATTATCGGCAAGCACTACTCTAAGCACTTGCGAATTAGAGATCATCATCATAAGAAGAAGCAGGAGAAGAAGAATGTATCGGGGGCTTTTGTTATGATGGGGCGCACCTTCTCTAACAAGTTTCATCAATTTAGAAATTATCACAAGAAGAAGAATATACTGGGGGGTGTTAGGGGCTACAGCTTGCATAAGAAGTGCTTGCGTCACTATACAAATTATCATCCTACGAAGAATACAAGGACAAGGGATACGACTTGGAAGAAGGTGTGGCATGCCCTGCAAAACTACTTGGTTAATAATAATGGAGATGATGAAGAGACTGGTACTAGTGATAAGGATGATGATAATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATCTGTACGACTGCGGTTTATATGTGATATCTGTTGCTAGAGTAATCTGTCACTGGTACTACGACGAGGAGGAACAGCAGCAGCAGAATTGCTGCTTCTTGGGGGACGACCGGTTTCCCAATATTATGAAGCATCTTGATAACTCTATAGAATACGCAATGCGCCCTCAATTGTTGAGGTTGATTCAACGCCTAAAACGTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

359

Amino Acids

41.98

Weight (kDa)

8.65

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AciI CCGC 2 cut(s) 294, 885
AclI AACGTT 1 cut(s) 443
AclWI GGATC 2 cut(s) 12, 824
AcsI RAATTY 3 cut(s) 98, 178, 187
AfaI GTAC 3 cut(s) 805, 878, 929
AfiI CCNNNNNNNGG 2 cut(s) 446, 474
AgeI ACCGGT 1 cut(s) 981
AgsI TTSAA 5 cut(s) 82, 98, 109, 370, 1058
AhlI ACTAGT 2 cut(s) 452, 806
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 4 cut(s) 357, 375, 422, 663
AluI AGCT 4 cut(s) 357, 375, 422, 663
Alw26I GTCTC 3 cut(s) 166, 201, 791
AlwI GGATC 2 cut(s) 12, 824
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 6 cut(s) 419, 422, 425, 949, 952, 963
ApoI RAATTY 3 cut(s) 98, 178, 187
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AsiGI ACCGGT 1 cut(s) 981
AspLEI GCGC 2 cut(s) 585, 1035
AspS9I GGNCC 2 cut(s) 13, 68
AsuHPI GGTGA 3 cut(s) 144, 245, 271
AvaII GGWCC 1 cut(s) 13
BamHI GGATCC 1 cut(s) 4
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 6 cut(s) 409, 431, 437, 950, 961, 964
BccI CCATC 2 cut(s) 284, 571
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 166, 201, 791
BcuI ACTAGT 2 cut(s) 452, 806
BfaI CTAG 4 cut(s) 453, 474, 807, 909
BfmI CTRYAG 4 cut(s) 156, 423, 658, 1017
BfuI GTATCC 1 cut(s) 721
BisI GCNGC 6 cut(s) 420, 423, 426, 950, 953, 964
BlsI GCNGC 6 cut(s) 421, 424, 427, 951, 954, 965
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 2 cut(s) 13, 68
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 385
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 1 cut(s) 1012
BmuI ACTGGG 1 cut(s) 650
Bpu10I CCTNAGC 1 cut(s) 861
BsaBI GATNNNNATC 2 cut(s) 525, 828
BsaJI CCNNGG 1 cut(s) 833
BsaWI WCCGGW 1 cut(s) 981
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 474
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 3 cut(s) 645, 805, 929
Bse3DI GCAATG 1 cut(s) 1035
Bse8I GATNNNNATC 2 cut(s) 525, 828
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 833
BseGI GGATG 3 cut(s) 138, 700, 823
BseJI GATNNNNATC 2 cut(s) 525, 828
BseLI CCNNNNNNNGG 2 cut(s) 446, 474
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 3 cut(s) 29, 197, 875
BseNI ACTGG 3 cut(s) 645, 805, 929
BseRI GAGGAG 2 cut(s) 230, 953
BseXI GCAGC 6 cut(s) 409, 431, 437, 950, 961, 964
Bsh1285I CGRYCG 1 cut(s) 982
BshFI GGCC 1 cut(s) 69
BshTI ACCGGT 1 cut(s) 981
BsiEI CGRYCG 1 cut(s) 982
BsiSI CCGG 1 cut(s) 982
BslFI GGGAC 2 cut(s) 463, 989
BslI CCNNNNNNNGG 2 cut(s) 446, 474
BsmAI GTCTC 3 cut(s) 166, 201, 791
BsmFI GGGAC 2 cut(s) 463, 989
BsnI GGCC 1 cut(s) 69
Bsp143I GATC 3 cut(s) 4, 520, 829
BspACI CCGC 2 cut(s) 294, 885
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 3 cut(s) 30, 196, 874
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 2 cut(s) 12, 824
BsrDI GCAATG 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 981
BsrI ACTGG 3 cut(s) 645, 805, 929
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 1 cut(s) 833
BssMI GATC 3 cut(s) 4, 520, 829
BssT1I CCWWGG 1 cut(s) 833
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 160, 259
Bst6I CTCTTC 2 cut(s) 789, 840
BstAPI GCANNNNNTGC 1 cut(s) 757
BstC8I GCNNGC 5 cut(s) 174, 491, 665, 680, 753
BstDEI CTNAG 5 cut(s) 38, 62, 183, 501, 861
BstF5I GGATG 3 cut(s) 138, 700, 823
BstHHI GCGC 2 cut(s) 585, 1035
BstKTI GATC 3 cut(s) 7, 523, 832
BstMAI GTCTC 3 cut(s) 166, 201, 791
BstMBI GATC 3 cut(s) 4, 520, 829
BstMCI CGRYCG 1 cut(s) 982
BstMWI GCNNNNNNNGC 2 cut(s) 425, 757
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 4 cut(s) 156, 423, 658, 1017
BstV1I GCAGC 6 cut(s) 409, 431, 437, 950, 961, 964
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 1 cut(s) 69
BtsCI GGATG 3 cut(s) 138, 700, 823
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 56, 922
Cac8I GCNNGC 5 cut(s) 174, 491, 665, 680, 753
CfoI GCGC 2 cut(s) 585, 1035
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 2 cut(s) 13, 68
CseI GACGC 1 cut(s) 671
Csp6I GTAC 3 cut(s) 804, 877, 928
CspAI ACCGGT 1 cut(s) 981
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviAII CATG 2 cut(s) 277, 752
CviQI GTAC 3 cut(s) 804, 877, 928
DdeI CTNAG 5 cut(s) 38, 62, 183, 501, 861
DpnI GATC 3 cut(s) 6, 522, 831
DpnII GATC 3 cut(s) 4, 520, 829
Eam1104I CTCTTC 2 cut(s) 789, 840
EarI CTCTTC 2 cut(s) 789, 840
EciI GGCGGA 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 833
Eco32I GATATC 1 cut(s) 900
Eco47I GGWCC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 2 cut(s) 98, 187
EcoRII CCWGG 1 cut(s) 383
EcoRV GATATC 1 cut(s) 900
EcoT14I CCWWGG 1 cut(s) 833
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 833
FaeI CATG 2 cut(s) 280, 755
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FaqI GGGAC 2 cut(s) 463, 989
FatI CATG 2 cut(s) 276, 751
Fnu4HI GCNGC 6 cut(s) 420, 423, 426, 950, 953, 964
FokI GGATG 3 cut(s) 125, 687, 830
Fsp4HI GCNGC 6 cut(s) 420, 423, 426, 950, 953, 964
FspBI CTAG 4 cut(s) 453, 474, 807, 909
GlaI GCGC 2 cut(s) 584, 1034
GluI GCNGC 6 cut(s) 420, 423, 426, 950, 953, 964
HaeIII GGCC 1 cut(s) 69
HapII CCGG 1 cut(s) 982
HgaI GACGC 1 cut(s) 671
HhaI GCGC 2 cut(s) 585, 1035
Hin1II CATG 2 cut(s) 280, 755
Hin6I GCGC 2 cut(s) 583, 1033
HinP1I GCGC 2 cut(s) 583, 1033
HinfI GANTC 2 cut(s) 218, 1054
HpaII CCGG 1 cut(s) 982
HphI GGTGA 3 cut(s) 144, 245, 271
Hpy188I TCNGA 3 cut(s) 9, 186, 193
Hpy188III TCNNGA 2 cut(s) 272, 1007
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 4 cut(s) 4, 152, 598, 736
HpyCH4III ACNGT 2 cut(s) 160, 259
HpyCH4IV ACGT 2 cut(s) 443, 1069
HpyCH4V TGCA 6 cut(s) 90, 338, 346, 425, 667, 760
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 757
HpyF3I CTNAG 5 cut(s) 38, 62, 183, 501, 861
HpySE526I ACGT 2 cut(s) 443, 1069
Hsp92II CATG 2 cut(s) 280, 755
HspAI GCGC 2 cut(s) 583, 1033
Kzo9I GATC 3 cut(s) 4, 520, 829
LmnI GCTCC 1 cut(s) 398
Lsp1109I GCAGC 6 cut(s) 409, 431, 437, 950, 961, 964
LweI GCATC 1 cut(s) 1012
MaeI CTAG 4 cut(s) 453, 474, 807, 909
MaeII ACGT 2 cut(s) 443, 1069
MaeIII GTNAC 3 cut(s) 47, 683, 920
MalI GATC 3 cut(s) 6, 522, 831
MboI GATC 3 cut(s) 4, 520, 829
MfeI CAATTG 1 cut(s) 1040
MflI RGATCY 1 cut(s) 4
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 8 cut(s) 59, 187, 208, 870, 931, 934, 1041, 1047
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 405, 774
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 982
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 425, 757
NdeII GATC 3 cut(s) 4, 520, 829
NlaIII CATG 2 cut(s) 280, 755
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 47, 683, 920
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 755
PaeI GCATGC 1 cut(s) 755
PcsI WCGNNNNNNNCGW 1 cut(s) 1066
PfeI GAWTC 1 cut(s) 1054
PflMI CCANNNNNTGG 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 981
PkrI GCNGC 6 cut(s) 421, 424, 427, 951, 954, 965
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 13
Psp1406I AACGTT 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 13, 68
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 3 cut(s) 805, 878, 929
RsaNI GTAC 3 cut(s) 804, 877, 928
SaqAI TTAA 2 cut(s) 405, 774
SatI GCNGC 6 cut(s) 420, 423, 426, 950, 953, 964
Sau3AI GATC 3 cut(s) 4, 520, 829
Sau96I GGNCC 2 cut(s) 13, 68
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 385
SfaNI GCATC 1 cut(s) 1012
SfcI CTRYAG 4 cut(s) 156, 423, 658, 1017
SinI GGWCC 1 cut(s) 13
SpeI ACTAGT 2 cut(s) 452, 806
SphI GCATGC 1 cut(s) 755
SsiI CCGC 2 cut(s) 294, 885
SspI AATATT 1 cut(s) 994
SspMI CTAG 4 cut(s) 453, 474, 807, 909
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 833
TaaI ACNGT 2 cut(s) 160, 259
TaiI ACGT 2 cut(s) 446, 1072
TaqI TCGA 1 cut(s) 213
TfiI GAWTC 1 cut(s) 1054
Tru1I TTAA 2 cut(s) 405, 774
Tru9I TTAA 2 cut(s) 405, 774
TscAI CASTG 2 cut(s) 63, 929
TseFI GTSAC 3 cut(s) 47, 683, 920
TseI GCWGC 6 cut(s) 419, 422, 425, 949, 952, 963
Tsp45I GTSAC 3 cut(s) 47, 683, 920
TspDTI ATGAA 3 cut(s) 593, 807, 1013
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 2 cut(s) 63, 929
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 3 cut(s) 98, 178, 187
XceI RCATGY 1 cut(s) 755
XspI CTAG 4 cut(s) 453, 474, 807, 909
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.