Rh4CG465100
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011558
Physical Location & Seq
Forward (+)
69492076 .. 69493481
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG465100.1

Sequence Viewer

Length: 1104 bp
ATGGATCCGAAGGTCCTTACTTACAAAAGTGTTGTGCTGAGAATGAGCGACTTGGCTCTGCTTAGAGGCCCACATTACTTGAACGATTGCATCATTGAATTCTATTTCAGTTATCTTTCTTCCCTTTGTGATGATGACATCCTTCTGGTTTCACCTACAGTCTCATTTTGGCTGGCAAATTCTCAGAATTCGGAGGCTACTTTTAGAGACTTCGAGGAGTCTAATCAACTTGGTGAAAAGCAAGTTGTGATATTCACGGTGAATGATAATCAAGACATGAGCCAAAATGACGGCGGAAACCATTGGAGTTTGCTGGTGTATTACAGAAAATCAAATGCATTTGTGCATTACGACAGCTTGGGGGGTTTGAATAACTTGGTTGCCAGGAAGTTTTACGGAGCAGTTAAGAAATATGTGGCAGCTGCAGCAACACAAACACCAACGATTGGGACTAGTAGTTTGGTTGCCACCACTAGGCCTATTATCGGCCACAAGTACTACTCTAAGCACTTGCGAATTAGAGATCATCATCATAAGAAGAAGCAGGAGAAGAAGAAAGTATCGGGGCCTTTTGTTATGATGGGGCGCACCTTCTCTAACAAGTTTCATCAATTTAGAAATTATCACAAGAAGAAGAATATACTGGGGGGTTTTGTTAGGGGGCACAGCTTGCTTAAGAAGTGCTTGCGTCAGTATACAAATTATCATCCTACGAAGAATATAAGGACAAGGGATACGACTTGGAAGAAGGTGTGGCATGCCCTGCAAAACTACAAGGTCAATAATAATGGAGATGATGAAGAGACTGGTACTAGTGATAAGGATGATGATAATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATCTGTACGACTGCGGTTTATATGTGATGTCTGTTGCTAGAGTAATCTGTCACTGGTACTACGACGAGGAGGAACAGCAGCACCAGCAGCAGAATTGCTGCTTCTTGGGGGACGACCGGTTTCCCAATATTATGAAGCATCTTGATAACTCTATAGAATACGCAATGCGCCCTCAATTGTTGAGGTTGATTCAACGCCTAAAACGTAATATTCCGTCTTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

367

Amino Acids

42.92

Weight (kDa)

9.08

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AccI GTMKAC 1 cut(s) 695
AciI CCGC 2 cut(s) 294, 891
AclWI GGATC 2 cut(s) 12, 830
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 3 cut(s) 98, 178, 187
AfaI GTAC 4 cut(s) 497, 811, 884, 935
AfiI CCNNNNNNNGG 3 cut(s) 446, 474, 485
AflII CTTAAG 1 cut(s) 674
AgeI ACCGGT 1 cut(s) 993
AgsI TTSAA 5 cut(s) 82, 98, 370, 1070, 1098
AhlI ACTAGT 2 cut(s) 452, 812
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 3 cut(s) 357, 422, 669
AluI AGCT 3 cut(s) 357, 422, 669
Alw26I GTCTC 3 cut(s) 166, 201, 797
AlwI GGATC 2 cut(s) 12, 830
AoxI GGCC 4 cut(s) 67, 476, 487, 566
ApeKI GCWGC 6 cut(s) 419, 422, 425, 955, 964, 975
ApoI RAATTY 3 cut(s) 98, 178, 187
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AsiGI ACCGGT 1 cut(s) 993
AspLEI GCGC 2 cut(s) 588, 1047
AspS9I GGNCC 3 cut(s) 13, 68, 566
AsuHPI GGTGA 3 cut(s) 144, 245, 271
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 666
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 1086
BbvCI CCTCAGC 1 cut(s) 867
BbvI GCAGC 6 cut(s) 409, 431, 437, 962, 967, 976
BccI CCATC 1 cut(s) 574
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 727
BcoDI GTCTC 3 cut(s) 166, 201, 797
BcuI ACTAGT 2 cut(s) 452, 812
BfaI CTAG 4 cut(s) 453, 474, 813, 915
BfmI CTRYAG 3 cut(s) 156, 423, 1029
BfrI CTTAAG 1 cut(s) 674
BfuI GTATCC 1 cut(s) 727
BisI GCNGC 6 cut(s) 420, 423, 426, 956, 965, 976
BlsI GCNGC 6 cut(s) 421, 424, 427, 957, 966, 977
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 13, 68, 566
BmiI GGNNCC 2 cut(s) 6, 567
BmrFI CCNGG 1 cut(s) 385
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 2 cut(s) 99, 1024
BmuI ACTGGG 1 cut(s) 653
BpiI GAAGAC 1 cut(s) 1086
Bpu10I CCTNAGC 1 cut(s) 867
BsaBI GATNNNNATC 2 cut(s) 528, 834
BsaJI CCNNGG 1 cut(s) 839
BsaWI WCCGGW 1 cut(s) 993
Bsc4I CCNNNNNNNGG 3 cut(s) 446, 474, 485
Bse118I RCCGGY 1 cut(s) 993
Bse1I ACTGG 3 cut(s) 648, 811, 935
Bse3DI GCAATG 1 cut(s) 1047
Bse8I GATNNNNATC 2 cut(s) 528, 834
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 839
BseGI GGATG 3 cut(s) 138, 706, 829
BseJI GATNNNNATC 2 cut(s) 528, 834
BseLI CCNNNNNNNGG 3 cut(s) 446, 474, 485
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 3 cut(s) 29, 197, 881
BseNI ACTGG 3 cut(s) 648, 811, 935
BseRI GAGGAG 2 cut(s) 230, 959
BseSI GKGCMC 1 cut(s) 666
BseXI GCAGC 6 cut(s) 409, 431, 437, 962, 967, 976
Bsh1285I CGRYCG 1 cut(s) 994
BshFI GGCC 4 cut(s) 69, 478, 489, 568
BshTI ACCGGT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 994
BsiSI CCGG 1 cut(s) 994
BslFI GGGAC 2 cut(s) 463, 1001
BslI CCNNNNNNNGG 3 cut(s) 446, 474, 485
BsmAI GTCTC 3 cut(s) 166, 201, 797
BsmFI GGGAC 2 cut(s) 463, 1001
BsnI GGCC 4 cut(s) 69, 478, 489, 568
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 3 cut(s) 4, 523, 835
BspACI CCGC 2 cut(s) 294, 891
BspANI GGCC 4 cut(s) 69, 478, 489, 568
BspCNI CTCAG 3 cut(s) 30, 196, 880
BspLI GGNNCC 2 cut(s) 6, 567
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 2 cut(s) 12, 830
BspTI CTTAAG 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 1 cut(s) 993
BsrI ACTGG 3 cut(s) 648, 811, 935
BssAI RCCGGY 1 cut(s) 993
BssECI CCNNGG 1 cut(s) 839
BssMI GATC 3 cut(s) 4, 523, 835
BssNAI GTATAC 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 839
Bst1107I GTATAC 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 160, 259
Bst6I CTCTTC 2 cut(s) 795, 846
BstAFI CTTAAG 1 cut(s) 674
BstAPI GCANNNNNTGC 2 cut(s) 670, 763
BstC8I GCNNGC 4 cut(s) 174, 671, 686, 759
BstDEI CTNAG 5 cut(s) 38, 62, 183, 504, 867
BstF5I GGATG 3 cut(s) 138, 706, 829
BstHHI GCGC 2 cut(s) 588, 1047
BstKTI GATC 3 cut(s) 7, 526, 838
BstMAI GTCTC 3 cut(s) 166, 201, 797
BstMBI GATC 3 cut(s) 4, 523, 835
BstMCI CGRYCG 1 cut(s) 994
BstMWI GCNNNNNNNGC 5 cut(s) 425, 670, 763, 961, 964
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 3 cut(s) 156, 423, 1029
BstSLI GKGCMC 1 cut(s) 666
BstV1I GCAGC 6 cut(s) 409, 431, 437, 962, 967, 976
BstV2I GAAGAC 1 cut(s) 1086
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 696
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 4 cut(s) 69, 478, 489, 568
BtsCI GGATG 3 cut(s) 138, 706, 829
BtsIMutI CAGTG 1 cut(s) 928
Cac8I GCNNGC 4 cut(s) 174, 671, 686, 759
CfoI GCGC 2 cut(s) 588, 1047
Cfr10I RCCGGY 1 cut(s) 993
Cfr13I GGNCC 3 cut(s) 13, 68, 566
CseI GACGC 1 cut(s) 677
Csp6I GTAC 4 cut(s) 496, 810, 883, 934
CspAI ACCGGT 1 cut(s) 993
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviAII CATG 2 cut(s) 277, 758
CviQI GTAC 4 cut(s) 496, 810, 883, 934
DdeI CTNAG 5 cut(s) 38, 62, 183, 504, 867
DpnI GATC 3 cut(s) 6, 525, 837
DpnII GATC 3 cut(s) 4, 523, 835
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 795, 846
EarI CTCTTC 2 cut(s) 795, 846
EciI GGCGGA 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 839
Eco147I AGGCCT 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 13
EcoO109I RGGNCCY 2 cut(s) 13, 566
EcoRI GAATTC 2 cut(s) 98, 187
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 839
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 839
FaeI CATG 2 cut(s) 280, 761
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 2 cut(s) 463, 1001
FatI CATG 2 cut(s) 276, 757
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 6 cut(s) 420, 423, 426, 956, 965, 976
FokI GGATG 3 cut(s) 125, 693, 836
Fsp4HI GCNGC 6 cut(s) 420, 423, 426, 956, 965, 976
FspBI CTAG 4 cut(s) 453, 474, 813, 915
GlaI GCGC 2 cut(s) 587, 1046
GluI GCNGC 6 cut(s) 420, 423, 426, 956, 965, 976
HaeIII GGCC 4 cut(s) 69, 478, 489, 568
HapII CCGG 1 cut(s) 994
HgaI GACGC 1 cut(s) 677
HhaI GCGC 2 cut(s) 588, 1047
Hin1II CATG 2 cut(s) 280, 761
Hin6I GCGC 2 cut(s) 586, 1045
HinP1I GCGC 2 cut(s) 586, 1045
HinfI GANTC 2 cut(s) 218, 1066
HpaII CCGG 1 cut(s) 994
HphI GGTGA 3 cut(s) 144, 245, 271
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 3 cut(s) 9, 186, 193
Hpy188III TCNNGA 2 cut(s) 272, 1019
Hpy8I GTNNAC 1 cut(s) 696
Hpy99I CGWCG 1 cut(s) 944
HpyAV CCTTC 4 cut(s) 4, 152, 601, 742
HpyCH4III ACNGT 2 cut(s) 160, 259
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 5 cut(s) 90, 338, 346, 425, 766
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 670, 763, 961, 964
HpyF3I CTNAG 5 cut(s) 38, 62, 183, 504, 867
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 2 cut(s) 280, 761
HspAI GCGC 2 cut(s) 586, 1045
Kzo9I GATC 3 cut(s) 4, 523, 835
LmnI GCTCC 1 cut(s) 398
Lsp1109I GCAGC 6 cut(s) 409, 431, 437, 962, 967, 976
LweI GCATC 2 cut(s) 99, 1024
MaeI CTAG 4 cut(s) 453, 474, 813, 915
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 1 cut(s) 926
MalI GATC 3 cut(s) 6, 525, 837
MboI GATC 3 cut(s) 4, 523, 835
MfeI CAATTG 1 cut(s) 1052
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 666
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 8 cut(s) 59, 187, 208, 876, 937, 940, 1053, 1059
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 405, 675
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 994
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 1 cut(s) 1052
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 5 cut(s) 425, 670, 763, 961, 964
NdeII GATC 3 cut(s) 4, 523, 835
NlaIII CATG 2 cut(s) 280, 761
NlaIV GGNNCC 2 cut(s) 6, 567
NmuCI GTSAC 1 cut(s) 926
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 761
PaeI GCATGC 1 cut(s) 761
PceI AGGCCT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 1078
PfeI GAWTC 1 cut(s) 1066
PflMI CCANNNNNTGG 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 993
PkrI GCNGC 6 cut(s) 421, 424, 427, 957, 966, 977
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 6, 567
PspPI GGNCC 3 cut(s) 13, 68, 566
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 4 cut(s) 497, 811, 884, 935
RsaNI GTAC 4 cut(s) 496, 810, 883, 934
SaqAI TTAA 2 cut(s) 405, 675
SatI GCNGC 6 cut(s) 420, 423, 426, 956, 965, 976
Sau3AI GATC 3 cut(s) 4, 523, 835
Sau96I GGNCC 3 cut(s) 13, 68, 566
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 2 cut(s) 99, 1024
SfcI CTRYAG 3 cut(s) 156, 423, 1029
SinI GGWCC 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SpeI ACTAGT 2 cut(s) 452, 812
SphI GCATGC 1 cut(s) 761
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 2 cut(s) 294, 891
SspI AATATT 2 cut(s) 1006, 1087
SspMI CTAG 4 cut(s) 453, 474, 813, 915
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 839
TaaI ACNGT 2 cut(s) 160, 259
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 1 cut(s) 213
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 1 cut(s) 1066
Tru1I TTAA 2 cut(s) 405, 675
Tru9I TTAA 2 cut(s) 405, 675
TscAI CASTG 1 cut(s) 935
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 6 cut(s) 419, 422, 425, 955, 964, 975
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 3 cut(s) 596, 813, 1025
TspGWI ACGGA 2 cut(s) 411, 1080
TspRI CASTG 1 cut(s) 935
Van91I CCANNNNNTGG 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 3 cut(s) 98, 178, 187
XceI RCATGY 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 4 cut(s) 453, 474, 813, 915
ZrmI AGTACT 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.