Rroxscaffold_5G00387380
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011558
Physical Location & Seq
Forward (+)
66040952 .. 66042100
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387380.1

Sequence Viewer

Length: 1149 bp
ATGGATCCGAGGGTCCTTACTTACAAAAGTGTTGTGCTGAGAATGAGCGACTTGGCTCTGCTTAGAGGCCCACATTACTTGAACGATTGCATCATTGAATTCTATTTCAGTTATCTTTCTTCCCTTTGTGATGATGACATCCTTCTGGTTTCACCTACAGTCTCATTTTGGCTGGCAAATTCTCAGAATTCGGAGGCTACTTTTACAGACTTCGAGGAGTCTAATCAACTTGGTGAAAAGCAAGTTGTGATATTCACGGTGAATGATAATCAAGACATGAGCCAAAATGACGGCGGAAACCATTGGAGTTTGCTGGTGTATTACAGAAAATCAAATGCATTTGTGCATTACGACAGCTTGGGGGGTTTGAATAACTTGGTTGCCAGGAAGTTTTACGGAGCAGTTAAGAAATATGTGGCAGCTGCAGCAACACAAACACCAACGTTTGGGACTAGTAGTTTGGTTGCCACCACTAGGTCTATTATCGGCCACAAGTACTTCTCTAAGCACTTGCGAATTAGAGATCATCATCATAACAAGTACTACTCTAAGCACTTGCGAATTAGAGATCATCATCATAAGAAGAAGCAGGGGAAGAAGAATGTATCGGGGGCTTTTGTTATGATGGGGCGCAGCTTCTCTAACAAGTTTCATCAATTTAGAAATTATCACAAGAAGAAGAATATATTGGGGGGTTTTGTTAGGGGCCATAGCTTGCTTAAGAAGTGCTTGCGTCAGTATACAAATTATCATCCTACGAAGAATATAAGGACAAGGGATACGACTTGGAAGAAGGTGTGGCATGCCCTGCAAAACTATTTGGTCAATAATAATGGAGATGATGAAGAGACTGGTACTAGTGATAAGGATGATGATAATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATCTGTACGACTGCGGTTTATATGTGATGTCTGTTGCTAGAGTAATCTGTCACTGGTACTACGACGAGGAGGAACAGCAGCACCAGCAGCAGAATTGCTGCTTCTTGGGGGACGACCGGTTTCCCAATATTATGAAGCATCTTGATAACTCTATAGAATACGCAATGCGCCCTCAATTGTTGAGGTTGATTCAACGCCTAAAACGTAATATTCCGTCTTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

382

Amino Acids

44.74

Weight (kDa)

9.15

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AccI GTMKAC 1 cut(s) 740
AciI CCGC 2 cut(s) 294, 936
AclI AACGTT 1 cut(s) 443
AclWI GGATC 2 cut(s) 12, 875
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 3 cut(s) 98, 178, 187
AfaI GTAC 5 cut(s) 497, 542, 856, 929, 980
AfiI CCNNNNNNNGG 2 cut(s) 446, 474
AflII CTTAAG 1 cut(s) 719
AgeI ACCGGT 1 cut(s) 1038
AgsI TTSAA 5 cut(s) 82, 98, 370, 1115, 1143
AhlI ACTAGT 2 cut(s) 452, 857
AjnI CCWGG 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 4 cut(s) 357, 422, 636, 714
AluI AGCT 4 cut(s) 357, 422, 636, 714
Alw26I GTCTC 2 cut(s) 166, 842
AlwI GGATC 2 cut(s) 12, 875
AoxI GGCC 3 cut(s) 67, 487, 706
ApeKI GCWGC 7 cut(s) 419, 422, 425, 633, 1000, 1009, 1020
ApoI RAATTY 3 cut(s) 98, 178, 187
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AsiGI ACCGGT 1 cut(s) 1038
AspLEI GCGC 2 cut(s) 633, 1092
AspS9I GGNCC 3 cut(s) 13, 68, 706
AsuHPI GGTGA 3 cut(s) 144, 245, 271
AvaII GGWCC 1 cut(s) 13
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 1131
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 7 cut(s) 409, 431, 437, 645, 1007, 1012, 1021
BccI CCATC 1 cut(s) 619
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 166, 842
BcuI ACTAGT 2 cut(s) 452, 857
BfaI CTAG 4 cut(s) 453, 474, 858, 960
BfmI CTRYAG 3 cut(s) 156, 423, 1074
BfrI CTTAAG 1 cut(s) 719
BfuI GTATCC 1 cut(s) 772
BisI GCNGC 7 cut(s) 420, 423, 426, 634, 1001, 1010, 1021
BlsI GCNGC 7 cut(s) 421, 424, 427, 635, 1002, 1011, 1022
BmcAI AGTACT 2 cut(s) 497, 542
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 13, 68, 706
BmiI GGNNCC 3 cut(s) 6, 14, 707
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 2 cut(s) 99, 1069
BpiI GAAGAC 1 cut(s) 1131
Bpu10I CCTNAGC 1 cut(s) 912
BsaBI GATNNNNATC 3 cut(s) 528, 573, 879
BsaJI CCNNGG 2 cut(s) 8, 884
BsaWI WCCGGW 1 cut(s) 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 474
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 2 cut(s) 856, 980
Bse3DI GCAATG 1 cut(s) 1092
Bse8I GATNNNNATC 3 cut(s) 528, 573, 879
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 8, 884
BseGI GGATG 3 cut(s) 138, 751, 874
BseJI GATNNNNATC 3 cut(s) 528, 573, 879
BseLI CCNNNNNNNGG 2 cut(s) 446, 474
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 3 cut(s) 29, 197, 926
BseNI ACTGG 2 cut(s) 856, 980
BseRI GAGGAG 2 cut(s) 230, 1004
BseXI GCAGC 7 cut(s) 409, 431, 437, 645, 1007, 1012, 1021
Bsh1285I CGRYCG 1 cut(s) 1039
BshFI GGCC 3 cut(s) 69, 489, 708
BshTI ACCGGT 1 cut(s) 1038
BsiEI CGRYCG 1 cut(s) 1039
BsiSI CCGG 1 cut(s) 1039
BslFI GGGAC 2 cut(s) 463, 1046
BslI CCNNNNNNNGG 2 cut(s) 446, 474
BsmAI GTCTC 2 cut(s) 166, 842
BsmFI GGGAC 2 cut(s) 463, 1046
BsnI GGCC 3 cut(s) 69, 489, 708
Bsp143I GATC 4 cut(s) 4, 523, 568, 880
BspACI CCGC 2 cut(s) 294, 936
BspANI GGCC 3 cut(s) 69, 489, 708
BspCNI CTCAG 3 cut(s) 30, 196, 925
BspLI GGNNCC 3 cut(s) 6, 14, 707
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 2 cut(s) 12, 875
BspTI CTTAAG 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 1092
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 2 cut(s) 856, 980
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 2 cut(s) 8, 884
BssMI GATC 4 cut(s) 4, 523, 568, 880
BssNAI GTATAC 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 884
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 160, 259
Bst6I CTCTTC 2 cut(s) 840, 891
BstAFI CTTAAG 1 cut(s) 719
BstAPI GCANNNNNTGC 1 cut(s) 808
BstC8I GCNNGC 4 cut(s) 174, 716, 731, 804
BstDEI CTNAG 6 cut(s) 38, 62, 183, 504, 549, 912
BstF5I GGATG 3 cut(s) 138, 751, 874
BstHHI GCGC 2 cut(s) 633, 1092
BstKTI GATC 4 cut(s) 7, 526, 571, 883
BstMAI GTCTC 2 cut(s) 166, 842
BstMBI GATC 4 cut(s) 4, 523, 568, 880
BstMCI CGRYCG 1 cut(s) 1039
BstMWI GCNNNNNNNGC 4 cut(s) 425, 808, 1006, 1009
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 3 cut(s) 156, 423, 1074
BstV1I GCAGC 7 cut(s) 409, 431, 437, 645, 1007, 1012, 1021
BstV2I GAAGAC 1 cut(s) 1131
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 741
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 3 cut(s) 69, 489, 708
BtsCI GGATG 3 cut(s) 138, 751, 874
BtsIMutI CAGTG 1 cut(s) 973
Cac8I GCNNGC 4 cut(s) 174, 716, 731, 804
CfoI GCGC 2 cut(s) 633, 1092
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 3 cut(s) 13, 68, 706
CseI GACGC 1 cut(s) 722
Csp6I GTAC 5 cut(s) 496, 541, 855, 928, 979
CspAI ACCGGT 1 cut(s) 1038
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviAII CATG 2 cut(s) 277, 803
CviQI GTAC 5 cut(s) 496, 541, 855, 928, 979
DdeI CTNAG 6 cut(s) 38, 62, 183, 504, 549, 912
DpnI GATC 4 cut(s) 6, 525, 570, 882
DpnII GATC 4 cut(s) 4, 523, 568, 880
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 840, 891
EarI CTCTTC 2 cut(s) 840, 891
EciI GGCGGA 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 884
Eco47I GGWCC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 2 cut(s) 98, 187
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 884
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 884
FaeI CATG 2 cut(s) 280, 806
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 2 cut(s) 463, 1046
FatI CATG 2 cut(s) 276, 802
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 7 cut(s) 420, 423, 426, 634, 1001, 1010, 1021
FokI GGATG 3 cut(s) 125, 738, 881
Fsp4HI GCNGC 7 cut(s) 420, 423, 426, 634, 1001, 1010, 1021
FspBI CTAG 4 cut(s) 453, 474, 858, 960
GlaI GCGC 2 cut(s) 632, 1091
GluI GCNGC 7 cut(s) 420, 423, 426, 634, 1001, 1010, 1021
HaeIII GGCC 3 cut(s) 69, 489, 708
HapII CCGG 1 cut(s) 1039
HgaI GACGC 1 cut(s) 722
HhaI GCGC 2 cut(s) 633, 1092
Hin1II CATG 2 cut(s) 280, 806
Hin6I GCGC 2 cut(s) 631, 1090
HinP1I GCGC 2 cut(s) 631, 1090
HinfI GANTC 2 cut(s) 218, 1111
HpaII CCGG 1 cut(s) 1039
HphI GGTGA 3 cut(s) 144, 245, 271
Hpy166II GTNNAC 1 cut(s) 741
Hpy188I TCNGA 3 cut(s) 9, 186, 193
Hpy188III TCNNGA 2 cut(s) 272, 1064
Hpy8I GTNNAC 1 cut(s) 741
Hpy99I CGWCG 1 cut(s) 989
HpyAV CCTTC 2 cut(s) 152, 787
HpyCH4III ACNGT 2 cut(s) 160, 259
HpyCH4IV ACGT 2 cut(s) 443, 1126
HpyCH4V TGCA 5 cut(s) 90, 338, 346, 425, 811
HpyF10VI GCNNNNNNNGC 4 cut(s) 425, 808, 1006, 1009
HpyF3I CTNAG 6 cut(s) 38, 62, 183, 504, 549, 912
HpySE526I ACGT 2 cut(s) 443, 1126
Hsp92II CATG 2 cut(s) 280, 806
HspAI GCGC 2 cut(s) 631, 1090
Kzo9I GATC 4 cut(s) 4, 523, 568, 880
LmnI GCTCC 1 cut(s) 398
Lsp1109I GCAGC 7 cut(s) 409, 431, 437, 645, 1007, 1012, 1021
LweI GCATC 2 cut(s) 99, 1069
MaeI CTAG 4 cut(s) 453, 474, 858, 960
MaeII ACGT 2 cut(s) 443, 1126
MaeIII GTNAC 1 cut(s) 971
MalI GATC 4 cut(s) 6, 525, 570, 882
MboI GATC 4 cut(s) 4, 523, 568, 880
MfeI CAATTG 1 cut(s) 1097
MflI RGATCY 1 cut(s) 4
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 9 cut(s) 3, 59, 187, 208, 921, 982, 985, 1098, 1104
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 405, 720
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 1 cut(s) 1039
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 1 cut(s) 1097
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 4 cut(s) 425, 808, 1006, 1009
NdeII GATC 4 cut(s) 4, 523, 568, 880
NlaIII CATG 2 cut(s) 280, 806
NlaIV GGNNCC 3 cut(s) 6, 14, 707
NmuCI GTSAC 1 cut(s) 971
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 806
PaeI GCATGC 1 cut(s) 806
PcsI WCGNNNNNNNCGW 1 cut(s) 1123
PfeI GAWTC 1 cut(s) 1111
PflMI CCANNNNNTGG 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 1038
PkrI GCNGC 7 cut(s) 421, 424, 427, 635, 1002, 1011, 1022
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 13
Psp1406I AACGTT 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 6, 14, 707
PspPI GGNCC 3 cut(s) 13, 68, 706
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 5 cut(s) 497, 542, 856, 929, 980
RsaNI GTAC 5 cut(s) 496, 541, 855, 928, 979
SaqAI TTAA 2 cut(s) 405, 720
SatI GCNGC 7 cut(s) 420, 423, 426, 634, 1001, 1010, 1021
Sau3AI GATC 4 cut(s) 4, 523, 568, 880
Sau96I GGNCC 3 cut(s) 13, 68, 706
ScaI AGTACT 2 cut(s) 497, 542
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 385
SfaNI GCATC 2 cut(s) 99, 1069
SfcI CTRYAG 3 cut(s) 156, 423, 1074
SinI GGWCC 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
SpeI ACTAGT 2 cut(s) 452, 857
SphI GCATGC 1 cut(s) 806
SsiI CCGC 2 cut(s) 294, 936
SspI AATATT 2 cut(s) 1051, 1132
SspMI CTAG 4 cut(s) 453, 474, 858, 960
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 884
TaaI ACNGT 2 cut(s) 160, 259
TaiI ACGT 2 cut(s) 446, 1129
TaqI TCGA 1 cut(s) 213
TatI WGTACW 2 cut(s) 495, 540
TfiI GAWTC 1 cut(s) 1111
Tru1I TTAA 2 cut(s) 405, 720
Tru9I TTAA 2 cut(s) 405, 720
TscAI CASTG 1 cut(s) 980
TseFI GTSAC 1 cut(s) 971
TseI GCWGC 7 cut(s) 419, 422, 425, 633, 1000, 1009, 1020
Tsp45I GTSAC 1 cut(s) 971
TspDTI ATGAA 3 cut(s) 641, 858, 1070
TspGWI ACGGA 2 cut(s) 411, 1125
TspRI CASTG 1 cut(s) 980
Van91I CCANNNNNTGG 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 3 cut(s) 98, 178, 187
XceI RCATGY 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 740
XspI CTAG 4 cut(s) 453, 474, 858, 960
ZrmI AGTACT 2 cut(s) 497, 542
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.