Rw4G037550
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011558
Physical Location & Seq
Forward (+)
64096049 .. 64097140
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037550.1

Sequence Viewer

Length: 1092 bp
ATGGATCCGAAGGTCCTTACTTACGAAAGTGTTGTGCTGAGAATGAGCGACTTGGCACTGCTTAGAGGCCCACATTACTTGAACGATTGCATAATTGAATTCTATTTCAGTTATCTTTCTTCCCTTTGTGATGATGACATCCTTCTGGTTTCACCTACAGTCTCATTTTGGCTAGCAAATTCTCAGAATTCGGAAGCTAATTTTAGAGACTTCGAGGAGTCTAATCAACTTGGTGAAAAGCAAGTTGTGATATTCACGGTGAATGATAATCAAGACATGAGCCAAAATGATGGCGGAAACCATTGGAGTTTGCTGGTGTATTACAGAAAATCCAATGCATTTGTGCATTACGACAGCTTGGGGGGTTTGAATAGCTTGGTTGCCAGGAAGTTTTACGGAGCAGTTAAGAAATATGTGGCAGCTGCAGCAACACAAACACCAACGGTTGGGACTAATAGTTTGGTTGACACCACTAGGTCTATTATCGGCAAGTACTACTCTAAGCACTTGCGAATTAGAGATCATCATCATAAGAAGAAGCAGGAGAAGAAGAATGTATCGGGGGCTTTAGTTATGATGGGGCGCACCTTCTCTAACAAGTTTCATCAATTTAGAAAGTATCACAAGAAGAAGAATATACTGGGGGGTTTTGTTAGGGGCCACAGCTTGCATAAGAAGTGCTTGCGTCACTATACAAATTATCATCCTACGAAGAATATAAGGACAAGGGATACGACTTGGAAGAAGGTGTGGCATGCCCTGCAAAACTACTTGGTCAATAATAATGGAGATGATGAAGAGACTGGTGCTAGTGATAAGGATGATGATAATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATCTGTACGACTGCGGTTTATATGTGATGTCTGTTGCTAGAGTAATCTGTCACTGGTACTACGACGAGGAGGAGGAGCAGCAGCAGCAGCAGCAGAATTGCTGCTTCTTGGGGGAAAAACGGTTTCCCAATATTATGAAGCATCTTGATAACTCTATAGAATACGCAATGCGCCCTCAATTGTTGAGGTTGATTCAACGCCTAAAACGTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

363

Amino Acids

42.52

Weight (kDa)

8.75

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AciI CCGC 2 cut(s) 294, 888
AclWI GGATC 2 cut(s) 12, 827
AcsI RAATTY 3 cut(s) 98, 178, 187
AfaI GTAC 3 cut(s) 494, 881, 932
AfiI CCNNNNNNNGG 1 cut(s) 446
AgsI TTSAA 4 cut(s) 82, 98, 370, 1070
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 5 cut(s) 197, 357, 375, 422, 666
AluI AGCT 5 cut(s) 197, 357, 375, 422, 666
Alw26I GTCTC 3 cut(s) 166, 201, 794
AlwI GGATC 2 cut(s) 12, 827
AoxI GGCC 2 cut(s) 67, 658
ApeKI GCWGC 9 cut(s) 419, 422, 425, 952, 955, 958, 961, 964, 975
ApoI RAATTY 3 cut(s) 98, 178, 187
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AspLEI GCGC 2 cut(s) 585, 1047
AspS9I GGNCC 3 cut(s) 13, 68, 658
AsuHPI GGTGA 3 cut(s) 144, 245, 271
AsuNHI GCTAGC 1 cut(s) 172
AvaII GGWCC 1 cut(s) 13
BamHI GGATCC 1 cut(s) 4
BbvCI CCTCAGC 1 cut(s) 864
BbvI GCAGC 9 cut(s) 409, 431, 437, 962, 964, 967, 970, 973, 976
BccI CCATC 2 cut(s) 284, 571
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 724
BcoDI GTCTC 3 cut(s) 166, 201, 794
BfaI CTAG 4 cut(s) 173, 474, 810, 912
BfmI CTRYAG 3 cut(s) 156, 423, 1029
BfuI GTATCC 1 cut(s) 724
BisI GCNGC 9 cut(s) 420, 423, 426, 953, 956, 959, 962, 965, 976
BlsI GCNGC 9 cut(s) 421, 424, 427, 954, 957, 960, 963, 966, 977
BmcAI AGTACT 1 cut(s) 494
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 13, 68, 658
BmiI GGNNCC 2 cut(s) 6, 659
BmrFI CCNGG 1 cut(s) 385
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 1 cut(s) 1024
BmtI GCTAGC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 650
Bpu10I CCTNAGC 1 cut(s) 864
BsaBI GATNNNNATC 2 cut(s) 525, 831
BsaJI CCNNGG 1 cut(s) 836
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 3 cut(s) 645, 808, 932
Bse3DI GCAATG 1 cut(s) 1047
Bse8I GATNNNNATC 2 cut(s) 525, 831
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 836
BseGI GGATG 3 cut(s) 138, 703, 826
BseJI GATNNNNATC 2 cut(s) 525, 831
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 3 cut(s) 29, 197, 878
BseNI ACTGG 3 cut(s) 645, 808, 932
BseRI GAGGAG 4 cut(s) 230, 956, 959, 962
BseXI GCAGC 9 cut(s) 409, 431, 437, 962, 964, 967, 970, 973, 976
BshFI GGCC 2 cut(s) 69, 660
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 3 cut(s) 166, 201, 794
BsmFI GGGAC 1 cut(s) 463
BsnI GGCC 2 cut(s) 69, 660
Bsp143I GATC 3 cut(s) 4, 520, 832
BspACI CCGC 2 cut(s) 294, 888
BspANI GGCC 2 cut(s) 69, 660
BspCNI CTCAG 3 cut(s) 30, 196, 877
BspLI GGNNCC 2 cut(s) 6, 659
BspMAI CTGCAG 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 2 cut(s) 12, 827
BsrDI GCAATG 1 cut(s) 1047
BsrI ACTGG 3 cut(s) 645, 808, 932
BssECI CCNNGG 1 cut(s) 836
BssMI GATC 3 cut(s) 4, 520, 832
BssT1I CCWWGG 1 cut(s) 836
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 4 cut(s) 160, 259, 445, 996
Bst6I CTCTTC 2 cut(s) 792, 843
BstAPI GCANNNNNTGC 1 cut(s) 760
BstC8I GCNNGC 4 cut(s) 174, 668, 683, 756
BstDEI CTNAG 5 cut(s) 38, 62, 183, 501, 864
BstF5I GGATG 3 cut(s) 138, 703, 826
BstHHI GCGC 2 cut(s) 585, 1047
BstKTI GATC 3 cut(s) 7, 523, 835
BstMAI GTCTC 3 cut(s) 166, 201, 794
BstMBI GATC 3 cut(s) 4, 520, 832
BstMWI GCNNNNNNNGC 5 cut(s) 425, 760, 958, 961, 964
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 3 cut(s) 156, 423, 1029
BstV1I GCAGC 9 cut(s) 409, 431, 437, 962, 964, 967, 970, 973, 976
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 724
BsuRI GGCC 2 cut(s) 69, 660
BtsCI GGATG 3 cut(s) 138, 703, 826
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 56, 925
Cac8I GCNNGC 4 cut(s) 174, 668, 683, 756
CfoI GCGC 2 cut(s) 585, 1047
Cfr13I GGNCC 3 cut(s) 13, 68, 658
CseI GACGC 1 cut(s) 674
Csp6I GTAC 3 cut(s) 493, 880, 931
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviAII CATG 2 cut(s) 277, 755
CviQI GTAC 3 cut(s) 493, 880, 931
DdeI CTNAG 5 cut(s) 38, 62, 183, 501, 864
DpnI GATC 3 cut(s) 6, 522, 834
DpnII GATC 3 cut(s) 4, 520, 832
Eam1104I CTCTTC 2 cut(s) 792, 843
EarI CTCTTC 2 cut(s) 792, 843
EciI GGCGGA 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 2 cut(s) 98, 187
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 836
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 836
FaeI CATG 2 cut(s) 280, 758
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 1 cut(s) 463
FatI CATG 2 cut(s) 276, 754
Fnu4HI GCNGC 9 cut(s) 420, 423, 426, 953, 956, 959, 962, 965, 976
FokI GGATG 3 cut(s) 125, 690, 833
Fsp4HI GCNGC 9 cut(s) 420, 423, 426, 953, 956, 959, 962, 965, 976
FspBI CTAG 4 cut(s) 173, 474, 810, 912
GlaI GCGC 2 cut(s) 584, 1046
GluI GCNGC 9 cut(s) 420, 423, 426, 953, 956, 959, 962, 965, 976
HaeIII GGCC 2 cut(s) 69, 660
HgaI GACGC 1 cut(s) 674
HhaI GCGC 2 cut(s) 585, 1047
Hin1II CATG 2 cut(s) 280, 758
Hin6I GCGC 2 cut(s) 583, 1045
HinP1I GCGC 2 cut(s) 583, 1045
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 2 cut(s) 218, 1066
HphI GGTGA 3 cut(s) 144, 245, 271
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 3 cut(s) 9, 186, 193
Hpy188III TCNNGA 2 cut(s) 272, 1019
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 1 cut(s) 941
HpyAV CCTTC 4 cut(s) 4, 152, 598, 739
HpyCH4III ACNGT 4 cut(s) 160, 259, 445, 996
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 6 cut(s) 90, 338, 346, 425, 670, 763
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 760, 958, 961, 964
HpyF3I CTNAG 5 cut(s) 38, 62, 183, 501, 864
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 2 cut(s) 280, 758
HspAI GCGC 2 cut(s) 583, 1045
Kzo9I GATC 3 cut(s) 4, 520, 832
LmnI GCTCC 2 cut(s) 398, 949
LpnPI CCDG 9 cut(s) 131, 299, 370, 397, 527, 626, 773, 789, 913
Lsp1109I GCAGC 9 cut(s) 409, 431, 437, 962, 964, 967, 970, 973, 976
LweI GCATC 1 cut(s) 1024
MaeI CTAG 4 cut(s) 173, 474, 810, 912
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 2 cut(s) 686, 923
MalI GATC 3 cut(s) 6, 522, 834
MboI GATC 3 cut(s) 4, 520, 832
MfeI CAATTG 1 cut(s) 1052
MflI RGATCY 1 cut(s) 4
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 8 cut(s) 59, 208, 873, 934, 937, 940, 1053, 1059
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 1 cut(s) 1052
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 5 cut(s) 425, 760, 958, 961, 964
NdeII GATC 3 cut(s) 4, 520, 832
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 2 cut(s) 280, 758
NlaIV GGNNCC 2 cut(s) 6, 659
NmuCI GTSAC 2 cut(s) 686, 923
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 758
PaeI GCATGC 1 cut(s) 758
PcsI WCGNNNNNNNCGW 1 cut(s) 1078
PfeI GAWTC 1 cut(s) 1066
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 9 cut(s) 421, 424, 427, 954, 957, 960, 963, 966, 977
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 6, 659
PspPI GGNCC 3 cut(s) 13, 68, 658
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 3 cut(s) 494, 881, 932
RsaNI GTAC 3 cut(s) 493, 880, 931
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 9 cut(s) 420, 423, 426, 953, 956, 959, 962, 965, 976
Sau3AI GATC 3 cut(s) 4, 520, 832
Sau96I GGNCC 3 cut(s) 13, 68, 658
ScaI AGTACT 1 cut(s) 494
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 385
SfaNI GCATC 1 cut(s) 1024
SfcI CTRYAG 3 cut(s) 156, 423, 1029
SinI GGWCC 1 cut(s) 13
SphI GCATGC 1 cut(s) 758
SsiI CCGC 2 cut(s) 294, 888
SspI AATATT 1 cut(s) 1006
SspMI CTAG 4 cut(s) 173, 474, 810, 912
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 836
TaaI ACNGT 4 cut(s) 160, 259, 445, 996
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 1 cut(s) 213
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 1 cut(s) 1066
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 63, 932
TseFI GTSAC 2 cut(s) 686, 923
TseI GCWGC 9 cut(s) 419, 422, 425, 952, 955, 958, 961, 964, 975
Tsp45I GTSAC 2 cut(s) 686, 923
TspDTI ATGAA 3 cut(s) 593, 810, 1025
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 2 cut(s) 63, 932
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 3 cut(s) 98, 178, 187
XceI RCATGY 1 cut(s) 758
XspI CTAG 4 cut(s) 173, 474, 810, 912
ZrmI AGTACT 1 cut(s) 494
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.