Rroxscaffold_2G00082780
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011558
Physical Location & Seq
Forward (+)
5541730 .. 5542410
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082780.1

Sequence Viewer

Length: 681 bp
ATGATAATGAAGACACGATCGAGTCAAAGCGACGGTGGAAAGCATTGGAGCTTGCTTGTGTATTACAGAAAATCGAATACATTCGTGCATTACGACAGCTTTGGGGGTTTCAATAGTTTGGTTGCCAGGAAGTTGTATCGAGCGGTTAAGAACTGCGTGGCAGCTCCAGCAACACAAGCACCAACTCCAGCTGGGACTAGTAGTTTGGTTACCCACATTAGTTCTACAATGGGACACAAGTACTACTCTAAGCACTTGCGGCGAATTATAAATTATCCCAACAAGAAGAATATGCTGGTGTCTTTTGTGATCGGATACATCTTCTCAAAGAAGTACTTGCGTCGGTTTATAAATTATCATCCTGTGAAGGTGTGTTATGCCCTGCAAAAGTACATCATAAGTAATAATGGAGATGATGAAGAGACTATCGATAGGGATGATGATGATGATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATTGGTACGACTGCGGTTTATATATGATGGCTATTGCTAGAGTAATCTGTCAATGGTACTACGATGAGGAGAGGGCGGAGCAAGAGCAGCAGAATTGCTTCTTGAAGGACGACCGGTTTCTGAATATCGCGAAGCATCTTGACAATTCTTTAGAATACACGATGCGCTCTGAGATGTCGAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

226

Amino Acids

26.59

Weight (kDa)

8.47

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 269, 350
AccBSI CCGCTC 1 cut(s) 143
AccII CGCG 1 cut(s) 623
AciI CCGC 4 cut(s) 143, 259, 507, 569
AclWI GGATC 1 cut(s) 446
AfaI GTAC 5 cut(s) 242, 335, 392, 500, 551
AgeI ACCGGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 112, 598
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 4 cut(s) 51, 99, 164, 191
AluI AGCT 4 cut(s) 51, 99, 164, 191
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 161, 580
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
AsiGI ACCGGT 1 cut(s) 606
Asp700I GAANNNNTTC 2 cut(s) 80, 590
AspLEI GCGC 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 173, 592
BccI CCATC 1 cut(s) 514
BciT130I CCWGG 1 cut(s) 127
BciVI GTATCC 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 416
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 531
BfuI GTATCC 1 cut(s) 308
BisI GCNGC 3 cut(s) 162, 260, 581
BlsI GCNGC 3 cut(s) 163, 261, 582
BmcAI AGTACT 2 cut(s) 242, 335
Bme1390I CCNGG 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 127
BmsI GCATC 2 cut(s) 637, 645
BpiI GAAGAC 1 cut(s) 17
BpmI CTGGAG 2 cut(s) 150, 171
Bpu10I CCTNAGC 1 cut(s) 483
Bsa29I ATCGAT 1 cut(s) 429
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 455
BsaWI WCCGGW 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 606
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 1 cut(s) 127
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 455
BseGI GGATG 2 cut(s) 358, 442
BseJI GATNNNNATC 1 cut(s) 450
BseMII CTCAG 2 cut(s) 497, 654
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 2 cut(s) 173, 592
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 623
Bsh1285I CGRYCG 2 cut(s) 20, 607
BshTI ACCGGT 1 cut(s) 606
BshVI ATCGAT 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 20, 607
BsiSI CCGG 1 cut(s) 607
BslFI GGGAC 2 cut(s) 208, 246
BsmAI GTCTC 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 208, 246
Bsp143I GATC 3 cut(s) 17, 309, 451
Bsp68I TCGCGA 1 cut(s) 623
BspACI CCGC 4 cut(s) 143, 259, 507, 569
BspCNI CTCAG 2 cut(s) 496, 655
BspDI ATCGAT 1 cut(s) 429
BspFNI CGCG 1 cut(s) 623
BspPI GGATC 1 cut(s) 446
BsrBI CCGCTC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 3 cut(s) 17, 309, 451
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 2 cut(s) 414, 462
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 3 cut(s) 249, 483, 663
BstEII GGTNACC 1 cut(s) 208
BstF5I GGATG 2 cut(s) 358, 442
BstFNI CGCG 1 cut(s) 623
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 3 cut(s) 20, 312, 454
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 17, 309, 451
BstMCI CGRYCG 2 cut(s) 20, 607
BstMWI GCNNNNNNNGC 4 cut(s) 167, 176, 259, 580
BstNI CCWGG 1 cut(s) 127
BstPI GGTNACC 1 cut(s) 208
BstSCI CCNGG 1 cut(s) 125
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 2 cut(s) 173, 592
BstV2I GAAGAC 1 cut(s) 17
Bsu15I ATCGAT 1 cut(s) 429
BsuI GTATCC 1 cut(s) 308
BsuTUI ATCGAT 1 cut(s) 429
BtsCI GGATG 2 cut(s) 358, 442
BtuMI TCGCGA 1 cut(s) 623
Cac8I GCNNGC 1 cut(s) 53
CfoI GCGC 1 cut(s) 660
Cfr10I RCCGGY 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 1 cut(s) 329
Csp6I GTAC 5 cut(s) 241, 334, 391, 499, 550
CspAI ACCGGT 1 cut(s) 606
CviJI RGCY 5 cut(s) 51, 99, 164, 191, 524
CviKI_1 RGCY 5 cut(s) 51, 99, 164, 191, 524
CviQI GTAC 5 cut(s) 241, 334, 391, 499, 550
DdeI CTNAG 3 cut(s) 249, 483, 663
DpnI GATC 3 cut(s) 19, 311, 453
DpnII GATC 3 cut(s) 17, 309, 451
Eam1104I CTCTTC 2 cut(s) 414, 462
EarI CTCTTC 2 cut(s) 414, 462
EciI GGCGGA 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 455
Eco91I GGTNACC 1 cut(s) 208
EcoO65I GGTNACC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 455
FaiI YATR 9 cut(s) 269, 293, 350, 378, 398, 479, 514, 516, 518
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 2 cut(s) 208, 246
Fnu4HI GCNGC 3 cut(s) 162, 260, 581
FokI GGATG 2 cut(s) 345, 449
Fsp4HI GCNGC 3 cut(s) 162, 260, 581
FspBI CTAG 2 cut(s) 198, 531
GlaI GCGC 1 cut(s) 659
GluI GCNGC 3 cut(s) 162, 260, 581
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 150, 171
HapII CCGG 1 cut(s) 607
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 660
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 607
Hpy188I TCNGA 3 cut(s) 314, 615, 664
Hpy188III TCNNGA 3 cut(s) 595, 622, 632
Hpy99I CGWCG 2 cut(s) 35, 345
HpyAV CCTTC 2 cut(s) 361, 592
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 88, 385
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 176, 259, 580
HpyF3I CTNAG 3 cut(s) 249, 483, 663
HspAI GCGC 1 cut(s) 658
Kzo9I GATC 3 cut(s) 17, 309, 451
LmnI GCTCC 3 cut(s) 48, 169, 571
LpnPI CCDG 9 cut(s) 112, 139, 177, 180, 201, 281, 375, 395, 620
Lsp1109I GCAGC 2 cut(s) 173, 592
LweI GCATC 2 cut(s) 637, 645
MaeI CTAG 2 cut(s) 198, 531
MaeIII GTNAC 1 cut(s) 208
MalI GATC 3 cut(s) 19, 311, 453
MbiI CCGCTC 1 cut(s) 143
MboI GATC 3 cut(s) 17, 309, 451
MboII GAAGA 5 cut(s) 22, 298, 313, 431, 479
MluCI AATT 6 cut(s) 264, 271, 352, 493, 586, 637
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 3 cut(s) 492, 553, 558
MroXI GAANNNNTTC 2 cut(s) 80, 590
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 607
MspR9I CCNGG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 4 cut(s) 167, 176, 259, 580
NdeII GATC 3 cut(s) 17, 309, 451
NruI TCGCGA 1 cut(s) 623
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 2 cut(s) 80, 590
PinAI ACCGGT 1 cut(s) 606
PkrI GCNGC 3 cut(s) 163, 261, 582
Ple19I CGATCG 1 cut(s) 20
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PsiI TTATAA 2 cut(s) 269, 350
Psp6I CCWGG 1 cut(s) 125
PspEI GGTNACC 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 125
PvuI CGATCG 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 191
RruI TCGCGA 1 cut(s) 623
RsaI GTAC 5 cut(s) 242, 335, 392, 500, 551
RsaNI GTAC 5 cut(s) 241, 334, 391, 499, 550
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 3 cut(s) 162, 260, 581
Sau3AI GATC 3 cut(s) 17, 309, 451
ScaI AGTACT 2 cut(s) 242, 335
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 127
SetI ASST 5 cut(s) 53, 101, 166, 193, 372
SfaNI GCATC 2 cut(s) 637, 645
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 6 cut(s) 264, 271, 352, 493, 586, 637
SsiI CCGC 4 cut(s) 143, 259, 507, 569
SspMI CTAG 2 cut(s) 198, 531
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 1 cut(s) 35
TaqI TCGA 5 cut(s) 20, 74, 139, 429, 671
TasI AATT 6 cut(s) 264, 271, 352, 493, 586, 637
TatI WGTACW 3 cut(s) 240, 333, 390
TauI GCSGC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseI GCWGC 2 cut(s) 161, 580
TspDTI ATGAA 2 cut(s) 23, 432
XmnI GAANNNNTTC 2 cut(s) 80, 590
XspI CTAG 2 cut(s) 198, 531
ZrmI AGTACT 2 cut(s) 242, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.