RLG00000021798
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011558
Physical Location & Seq
Reverse (-)
78384065 .. 78385007
943 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021798

Sequence Viewer

Length: 741 bp
ATGGATGAGGAGGTCGTGACTTATAATGGTGTTGTGCTGAAAAGGAGCGACTTGGATCTGACAAGAGTCAAAGCGACGGTGGAAAGCATTGGAGCTAGCTTGCTTGTGTATTACAGAAAATCGAATACATTCGTGCATTACGACAGCTTTGGGGGTTTAAATAGTTTGGTTGCCAGGAAGTTGTATCGAGCGGTTAAGAACTGCGTGGCAGCTCCAGCAACACAAGCACCAACTCCAGCTGGGACTAGTAGTTTAACTATAATGGGACACAAGTACTACTCTAAGCACCTGCGGCGAATTATAAATTATCCCAAGAAGAATATGCTGGTGTCTTTTGTGATCGGATACATCTTCTCAAAGAAGTACTTGCGTCGGTTTAAAAATTATCATCCTGTGAAGGTGTGCTATGCCCTGCAAAAGTACATCATAAGTAATAATGGAGATGATGAAGAGACTATCGATAGGGATGATGATGATGATGATCCTTGGTTTAGAGAAGAGAATGTTATGCCTCAGCAGACAAATTGGTACGACTGCGGTTTATATGTGATGGCTATTGCTAGAGTAATCTGTGAATGGTACTACGATGAGGAGAGGGCGGAGCAGGAGCAGGAGCAGGAGCAGCAGAATTGCTTCTTGAAGGACGACCGGGTTCCGCAGCTTGACAATTCTTTAGAATACACGATGCGCGCTGAGATGTTGAGACTCATTCAACACCACATCGAACTTAAATCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

247

Amino Acids

28.86

Weight (kDa)

6.1

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 24, 302
AarI CACCTGC 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 297
AccBSI CCGCTC 1 cut(s) 191
AccII CGCG 1 cut(s) 690
AciI CCGC 5 cut(s) 191, 292, 537, 599, 656
AclWI GGATC 2 cut(s) 63, 476
AfaI GTAC 5 cut(s) 275, 365, 422, 530, 581
AgsI TTSAA 2 cut(s) 640, 713
AhlI ACTAGT 1 cut(s) 245
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 6 cut(s) 95, 99, 147, 212, 239, 661
AluI AGCT 6 cut(s) 95, 99, 147, 212, 239, 661
Alw26I GTCTC 2 cut(s) 446, 697
AlwI GGATC 2 cut(s) 63, 476
ApeKI GCWGC 3 cut(s) 209, 622, 658
Asp700I GAANNNNTTC 2 cut(s) 128, 632
AspLEI GCGC 2 cut(s) 690, 692
AsuC2I CCSGG 1 cut(s) 650
AsuNHI GCTAGC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 221, 634, 670
BccI CCATC 1 cut(s) 544
BciT130I CCWGG 1 cut(s) 175
BciVI GTATCC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 650
BcoDI GTCTC 2 cut(s) 446, 697
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 4 cut(s) 96, 246, 561, 739
BfuAI ACCTGC 1 cut(s) 297
BfuI GTATCC 1 cut(s) 338
BisI GCNGC 4 cut(s) 210, 293, 623, 659
BlsI GCNGC 4 cut(s) 211, 294, 624, 660
BmcAI AGTACT 2 cut(s) 275, 365
Bme1390I CCNGG 2 cut(s) 175, 650
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 2 cut(s) 175, 650
BmsI GCATC 1 cut(s) 675
BmtI GCTAGC 1 cut(s) 99
BoxI GACNNNNGTC 1 cut(s) 65
BpmI CTGGAG 2 cut(s) 198, 219
Bpu10I CCTNAGC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 175
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 3 cut(s) 10, 388, 472
BseJI GATNNNNATC 1 cut(s) 480
BseMII CTCAG 2 cut(s) 527, 684
BsePI GCGCGC 1 cut(s) 688
BseRI GAGGAG 2 cut(s) 23, 605
BseXI GCAGC 3 cut(s) 221, 634, 670
BseYI CCCAGC 1 cut(s) 239
Bsh1236I CGCG 1 cut(s) 690
Bsh1285I CGRYCG 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 649
BsiSI CCGG 1 cut(s) 649
BslFI GGGAC 2 cut(s) 256, 279
BsmAI GTCTC 2 cut(s) 446, 697
BsmFI GGGAC 2 cut(s) 256, 279
Bsp143I GATC 3 cut(s) 55, 339, 481
BspACI CCGC 5 cut(s) 191, 292, 537, 599, 656
BspCNI CTCAG 2 cut(s) 526, 685
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 1 cut(s) 690
BspLI GGNNCC 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 297
BspOI GCTAGC 1 cut(s) 99
BspPI GGATC 2 cut(s) 63, 476
BsrBI CCGCTC 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 485
BssHII GCGCGC 1 cut(s) 688
BssMI GATC 3 cut(s) 55, 339, 481
BssT1I CCWWGG 1 cut(s) 485
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 2 cut(s) 444, 492
BstC8I GCNNGC 3 cut(s) 97, 101, 690
BstDEI CTNAG 3 cut(s) 282, 513, 693
BstF5I GGATG 3 cut(s) 10, 388, 472
BstFNI CGCG 1 cut(s) 690
BstHHI GCGC 2 cut(s) 690, 692
BstKTI GATC 3 cut(s) 58, 342, 484
BstMAI GTCTC 2 cut(s) 446, 697
BstMBI GATC 3 cut(s) 55, 339, 481
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 4 cut(s) 215, 224, 292, 622
BstNI CCWGG 1 cut(s) 175
BstPAI GACNNNNGTC 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 173, 648
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 3 cut(s) 221, 634, 670
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu15I ATCGAT 1 cut(s) 459
BsuI GTATCC 1 cut(s) 338
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 3 cut(s) 10, 388, 472
BveI ACCTGC 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 97, 101, 690
CfoI GCGC 2 cut(s) 690, 692
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 1 cut(s) 359
Csp6I GTAC 5 cut(s) 274, 364, 421, 529, 580
CviJI RGCY 7 cut(s) 95, 99, 147, 212, 239, 554, 661
CviKI_1 RGCY 7 cut(s) 95, 99, 147, 212, 239, 554, 661
CviQI GTAC 5 cut(s) 274, 364, 421, 529, 580
DdeI CTNAG 3 cut(s) 282, 513, 693
DpnI GATC 3 cut(s) 57, 341, 483
DpnII GATC 3 cut(s) 55, 339, 481
DraI TTTAAA 2 cut(s) 159, 379
Eam1104I CTCTTC 2 cut(s) 444, 492
EarI CTCTTC 2 cut(s) 444, 492
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaiI YATR 9 cut(s) 24, 260, 302, 323, 408, 428, 509, 544, 546
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 2 cut(s) 256, 279
Fnu4HI GCNGC 4 cut(s) 210, 293, 623, 659
FokI GGATG 3 cut(s) 17, 375, 479
Fsp4HI GCNGC 4 cut(s) 210, 293, 623, 659
FspBI CTAG 4 cut(s) 96, 246, 561, 739
GlaI GCGC 2 cut(s) 689, 691
GluI GCNGC 4 cut(s) 210, 293, 623, 659
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 2 cut(s) 198, 219
HapII CCGG 1 cut(s) 649
HgaI GACGC 1 cut(s) 359
HhaI GCGC 2 cut(s) 690, 692
Hin6I GCGC 2 cut(s) 688, 690
HinP1I GCGC 2 cut(s) 688, 690
HinfI GANTC 2 cut(s) 66, 705
HpaII CCGG 1 cut(s) 649
Hpy188I TCNGA 2 cut(s) 60, 344
Hpy188III TCNNGA 2 cut(s) 16, 637
Hpy99I CGWCG 2 cut(s) 79, 375
HpyAV CCTTC 2 cut(s) 391, 634
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 136, 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 224, 292, 622
HpyF3I CTNAG 3 cut(s) 282, 513, 693
HspAI GCGC 2 cut(s) 688, 690
Kzo9I GATC 3 cut(s) 55, 339, 481
LmnI GCTCC 7 cut(s) 45, 92, 217, 601, 607, 613, 619
Lsp1109I GCAGC 3 cut(s) 221, 634, 670
LweI GCATC 1 cut(s) 675
MaeI CTAG 4 cut(s) 96, 246, 561, 739
MaeIII GTNAC 1 cut(s) 16
MalI GATC 3 cut(s) 57, 341, 483
MbiI CCGCTC 1 cut(s) 191
MboI GATC 3 cut(s) 55, 339, 481
MboII GAAGA 4 cut(s) 328, 343, 461, 509
MflI RGATCY 1 cut(s) 55
MluCI AATT 6 cut(s) 297, 304, 382, 523, 628, 667
MlyI GAGTC 2 cut(s) 75, 699
MnlI CCTC 4 cut(s) 4, 522, 583, 588
MroXI GAANNNNTTC 2 cut(s) 128, 632
MseI TTAA 5 cut(s) 158, 195, 254, 378, 729
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 175, 650
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 4 cut(s) 215, 224, 292, 622
NciI CCSGG 1 cut(s) 650
NdeII GATC 3 cut(s) 55, 339, 481
NheI GCTAGC 1 cut(s) 95
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 1 cut(s) 16
PaqCI CACCTGC 1 cut(s) 297
PauI GCGCGC 1 cut(s) 688
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdmI GAANNNNTTC 2 cut(s) 128, 632
PkrI GCNGC 4 cut(s) 211, 294, 624, 660
PleI GAGTC 2 cut(s) 74, 699
PpsI GAGTC 2 cut(s) 74, 699
PshAI GACNNNNGTC 1 cut(s) 65
PsiI TTATAA 2 cut(s) 24, 302
Psp6I CCWGG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 654
PsuI RGATCY 1 cut(s) 55
PteI GCGCGC 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 5 cut(s) 275, 365, 422, 530, 581
RsaNI GTAC 5 cut(s) 274, 364, 421, 529, 580
SaqAI TTAA 5 cut(s) 158, 195, 254, 378, 729
SatI GCNGC 4 cut(s) 210, 293, 623, 659
Sau3AI GATC 3 cut(s) 55, 339, 481
ScaI AGTACT 2 cut(s) 275, 365
SchI GAGTC 2 cut(s) 75, 699
ScrFI CCNGG 2 cut(s) 175, 650
SetI ASST 9 cut(s) 15, 97, 101, 149, 214, 241, 291, 402, 663
SfaNI GCATC 1 cut(s) 675
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 6 cut(s) 297, 304, 382, 523, 628, 667
SsiI CCGC 5 cut(s) 191, 292, 537, 599, 656
SspMI CTAG 4 cut(s) 96, 246, 561, 739
StyD4I CCNGG 2 cut(s) 173, 648
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 4 cut(s) 122, 187, 459, 723
TasI AATT 6 cut(s) 297, 304, 382, 523, 628, 667
TatI WGTACW 3 cut(s) 273, 363, 420
TauI GCSGC 1 cut(s) 295
Tru1I TTAA 5 cut(s) 158, 195, 254, 378, 729
Tru9I TTAA 5 cut(s) 158, 195, 254, 378, 729
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 3 cut(s) 209, 622, 658
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 462
XmnI GAANNNNTTC 2 cut(s) 128, 632
XspI CTAG 4 cut(s) 96, 246, 561, 739
ZrmI AGTACT 2 cut(s) 275, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.