RchiOBHm_Chr5g0032021

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016970
Physical Location & Seq
Forward (+)
25710665 .. 25712881
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31122

Sequence Viewer

Length: 768 bp
ATGACCGTTGGTTTGGGGAGCCTGATCGACAGTTTATGGAGGCATGTATGGCTCTTGGTGCAAGGCCAATACGGTTCCCTGTACATCGTCGATCTGAGGGCCTGCTCATGGGTCGGGCGCGAGCGGCTGCAGCGTCTGATGGCGGTGGTGGTGTTGGTCAGTCGCACCAGAAGGACCAGATGGGAGTCTAGATCAGAATGGAGCTTGTGGATGATTTGGTCTTGGGCCCAATGTAGAGAAGTCCATTCGGGCTTGAGTTCTCAAAGGATTTGGGACCCAAGAGGTGTTTTGGGTATCTTTTTGATCGATTGTGTTATCCGTCTTATTTCAGGTATACCGTGTTTATGTGCAGGTAGATTGCTCATCTCTACATTCTATAAGAGATCTCAGTATAATATTGATGTGAGTACCACAATTGCTTGCCTGGCGAACAATGTTACTTGTGCTGGGACGAATATAGTTTTGATTGGTCTACCGACATCTTGCATGCCCGGTTTGCAGATTCTGATGGTTTGGTCTCCAAATCTCAAGGTCATGGCAATGGTCTCATTGTTGGTAATTATCTTGAAGAGGCTGGGAATTTACCTTCAGTTTTCTGTTAGTTTCTCTATAAGTGATTCAGAGCAATATTTTATTGTACATGAACAACCTGTTGGTGTAGTACACCTTTTGGTTTGTTCTAGCTTAGCTTTAGCTTCAAGCCGTATGTGGCTTGTGTCTGCTCCCAATGTTTTTCGGGATGCCATTCTAAACGATTGTAATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.79

Weight (kDa)

9.28

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 2 cut(s) 334, 472
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 124, 143
AcsI RAATTY 1 cut(s) 579
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 4 cut(s) 83, 409, 639, 663
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 2 cut(s) 568, 699
AjnI CCWGG 1 cut(s) 423
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 204, 684, 689, 695
AluI AGCT 4 cut(s) 204, 684, 689, 695
Alw26I GTCTC 2 cut(s) 522, 550
AlwNI CAGNNNCTG 2 cut(s) 136, 505
AoxI GGCC 3 cut(s) 64, 99, 225
ApaI GGGCCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 127, 130
ApoI RAATTY 1 cut(s) 579
ArsI GACNNNNNNTTYG 1 cut(s) 27
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 5 cut(s) 99, 174, 225, 226, 274
AsuC2I CCSGG 1 cut(s) 492
AvaII GGWCC 2 cut(s) 174, 274
BaeGI GKGCMC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 114, 142
BccI CCATC 3 cut(s) 133, 174, 502
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 1 cut(s) 425
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 2 cut(s) 522, 550
BfaI CTAG 2 cut(s) 189, 681
BfmI CTRYAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 383
BisI GCNGC 3 cut(s) 125, 128, 131
BlpI GCTNAGC 1 cut(s) 685
BlsI GCNGC 3 cut(s) 126, 129, 132
Bme1390I CCNGG 2 cut(s) 425, 492
Bme18I GGWCC 2 cut(s) 174, 274
BmgT120I GGNCC 5 cut(s) 99, 174, 225, 226, 274
BmiI GGNNCC 5 cut(s) 20, 76, 227, 275, 276
BmrFI CCNGG 2 cut(s) 425, 492
BmsI GCATC 1 cut(s) 730
Bpu1102I GCTNAGC 1 cut(s) 685
BpuEI CTTGAG 2 cut(s) 274, 512
BpuMI CCSGG 1 cut(s) 492
Bsa29I ATCGAT 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 759
BsaI GGTCTC 2 cut(s) 522, 550
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 425
BseCI ATCGAT 1 cut(s) 306
BseGI GGATG 2 cut(s) 216, 745
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 2 cut(s) 86, 401
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 2 cut(s) 114, 142
BseYI CCCAGC 2 cut(s) 446, 574
BsgI GTGCAG 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 66, 101, 227
BshVI ATCGAT 1 cut(s) 306
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 287, 463
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 522, 550
BsmFI GGGAC 2 cut(s) 287, 463
BsnI GGCC 3 cut(s) 66, 101, 227
Bso31I GGTCTC 2 cut(s) 522, 550
Bsp120I GGGCCC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 229
Bsp1407I TGTACA 2 cut(s) 81, 637
Bsp143I GATC 5 cut(s) 24, 91, 191, 303, 383
Bsp1720I GCTNAGC 1 cut(s) 685
BspACI CCGC 2 cut(s) 124, 143
BspANI GGCC 3 cut(s) 66, 101, 227
BspCNI CTCAG 2 cut(s) 87, 400
BspDI ATCGAT 1 cut(s) 306
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 5 cut(s) 20, 76, 227, 275, 276
BspMAI CTGCAG 1 cut(s) 132
BspMI ACCTGC 1 cut(s) 341
BspTNI GGTCTC 2 cut(s) 522, 550
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 546
BsrGI TGTACA 2 cut(s) 81, 637
BssMI GATC 5 cut(s) 24, 91, 191, 303, 383
BssNAI GTATAC 1 cut(s) 335
Bst1107I GTATAC 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 7, 32, 74, 339
Bst6I CTCTTC 1 cut(s) 563
BstAUI TGTACA 2 cut(s) 81, 637
BstC8I GCNNGC 4 cut(s) 103, 122, 421, 488
BstDEI CTNAG 3 cut(s) 95, 387, 685
BstF5I GGATG 2 cut(s) 216, 745
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 5 cut(s) 27, 94, 194, 306, 386
BstMAI GTCTC 2 cut(s) 522, 550
BstMBI GATC 5 cut(s) 24, 91, 191, 303, 383
BstMWI GCNNNNNNNGC 6 cut(s) 49, 58, 124, 130, 425, 496
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 2 cut(s) 47, 490
BstSCI CCNGG 2 cut(s) 423, 490
BstSFI CTRYAG 1 cut(s) 128
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 114, 142
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BstZ17I GTATAC 1 cut(s) 335
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 3 cut(s) 66, 101, 227
BsuTUI ATCGAT 1 cut(s) 306
BtsCI GGATG 2 cut(s) 216, 745
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 4 cut(s) 103, 122, 421, 488
CaiI CAGNNNCTG 2 cut(s) 136, 505
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 5 cut(s) 99, 174, 225, 226, 274
ClaI ATCGAT 1 cut(s) 306
CseI GACGC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 82, 408, 638, 662
CviAII CATG 5 cut(s) 44, 108, 487, 535, 641
CviQI GTAC 4 cut(s) 82, 408, 638, 662
DdeI CTNAG 3 cut(s) 95, 387, 685
DpnI GATC 5 cut(s) 26, 93, 193, 305, 385
DpnII GATC 5 cut(s) 24, 91, 191, 303, 383
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 2 cut(s) 522, 550
Eco47I GGWCC 2 cut(s) 174, 274
Eco57I CTGAAG 1 cut(s) 572
EcoO109I RGGNCCY 2 cut(s) 99, 274
EcoRII CCWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 229
FaeI CATG 5 cut(s) 47, 111, 490, 538, 644
FaqI GGGAC 2 cut(s) 287, 463
FatI CATG 5 cut(s) 43, 107, 486, 534, 640
FblI GTMKAC 2 cut(s) 334, 472
Fnu4HI GCNGC 3 cut(s) 125, 128, 131
FokI GGATG 2 cut(s) 223, 752
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 3 cut(s) 125, 128, 131
FspBI CTAG 2 cut(s) 189, 681
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 125, 128, 131
GsaI CCCAGC 2 cut(s) 450, 578
HaeIII GGCC 3 cut(s) 66, 101, 227
HapII CCGG 1 cut(s) 492
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 120
Hin1II CATG 5 cut(s) 47, 111, 490, 538, 644
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 185, 502, 617
HpaII CCGG 1 cut(s) 492
Hpy166II GTNNAC 3 cut(s) 335, 473, 664
Hpy188I TCNGA 5 cut(s) 96, 138, 196, 507, 622
Hpy188III TCNNGA 3 cut(s) 189, 565, 737
Hpy8I GTNNAC 3 cut(s) 335, 473, 664
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 165, 596
HpyCH4III ACNGT 4 cut(s) 7, 32, 74, 339
HpyCH4V TGCA 5 cut(s) 61, 130, 350, 486, 499
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 58, 124, 130, 425, 496
HpyF3I CTNAG 3 cut(s) 95, 387, 685
Hsp92II CATG 5 cut(s) 47, 111, 490, 538, 644
HspAI GCGC 1 cut(s) 118
KflI GGGWCCC 1 cut(s) 274
Kzo9I GATC 5 cut(s) 24, 91, 191, 303, 383
LmnI GCTCC 3 cut(s) 18, 201, 727
Lsp1109I GCAGC 2 cut(s) 114, 142
LweI GCATC 1 cut(s) 730
MaeI CTAG 2 cut(s) 189, 681
MaeIII GTNAC 1 cut(s) 436
MalI GATC 5 cut(s) 26, 93, 193, 305, 385
MbiI CCGCTC 1 cut(s) 124
MboI GATC 5 cut(s) 24, 91, 191, 303, 383
MboII GAAGA 1 cut(s) 580
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 414, 558, 579
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 4 cut(s) 33, 90, 275, 564
MseI TTAA 1 cut(s) 766
MslI CAYNNNNRTG 1 cut(s) 539
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 2 cut(s) 425, 492
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 49, 58, 124, 130, 425, 496
NciI CCSGG 1 cut(s) 492
NdeII GATC 5 cut(s) 24, 91, 191, 303, 383
NlaIII CATG 5 cut(s) 47, 111, 490, 538, 644
NlaIV GGNNCC 5 cut(s) 20, 76, 227, 275, 276
NspI RCATGY 2 cut(s) 47, 490
PaeI GCATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 502, 617
PkrI GCNGC 3 cut(s) 126, 129, 132
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 423
PspFI CCCAGC 2 cut(s) 446, 574
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 5 cut(s) 20, 76, 227, 275, 276
PspOMI GGGCCC 1 cut(s) 225
PspPI GGNCC 5 cut(s) 99, 174, 225, 226, 274
PspPPI RGGWCCY 1 cut(s) 274
PstI CTGCAG 1 cut(s) 132
PstNI CAGNNNCTG 2 cut(s) 136, 505
PsuI RGATCY 1 cut(s) 383
RsaI GTAC 4 cut(s) 83, 409, 639, 663
RsaNI GTAC 4 cut(s) 82, 408, 638, 662
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 1 cut(s) 766
SatI GCNGC 3 cut(s) 125, 128, 131
Sau3AI GATC 5 cut(s) 24, 91, 191, 303, 383
Sau96I GGNCC 5 cut(s) 99, 174, 225, 226, 274
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 425, 492
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 1 cut(s) 128
SinI GGWCC 2 cut(s) 174, 274
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 253, 527
SmoI CTYRAG 2 cut(s) 253, 527
SphI GCATGC 1 cut(s) 490
Sse9I AATT 3 cut(s) 414, 558, 579
SsiI CCGC 2 cut(s) 124, 143
SspI AATATT 2 cut(s) 397, 629
SspMI CTAG 2 cut(s) 189, 681
StyD4I CCNGG 2 cut(s) 423, 490
TaaI ACNGT 4 cut(s) 7, 32, 74, 339
TaqI TCGA 3 cut(s) 27, 90, 306
TasI AATT 3 cut(s) 414, 558, 579
TatI WGTACW 3 cut(s) 81, 637, 661
TauI GCSGC 1 cut(s) 127
TfiI GAWTC 2 cut(s) 502, 617
Tru1I TTAA 1 cut(s) 766
Tru9I TTAA 1 cut(s) 766
TseI GCWGC 2 cut(s) 127, 130
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 174, 274
XapI RAATTY 1 cut(s) 579
XbaI TCTAGA 1 cut(s) 188
XceI RCATGY 2 cut(s) 47, 490
XmiI GTMKAC 2 cut(s) 334, 472
XspI CTAG 2 cut(s) 189, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.