RLG00000003109

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016970
Physical Location & Seq
Reverse (-)
44465608 .. 44467516
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003109

Sequence Viewer

Length: 648 bp
ATGAAGCGGATTGGTAGCCATTTCAAGGAACAGAGCGCAAGAAAGAAGAAGAAGCAAAGGAAACAAGGGGAAGGAGAAGAGACTGATGGGAATTTGGGAAGAGGAACTAGGGAATATAAAGAGGTCTCTAGGGGGCAACAAAACGAGGGGGAAGGAGAGAATCGAAGAGATCAGGCATGGGAACAGAAAAGATTTCAAAACAGAAGGCTCATTAATGGTGAAAGGAAAGGCGGATTGGAGGATTTTCGATGGTGGAACCGGGCGAGTGCTTTCGAAGGTGACGCGAGATGGCGAGTTGCTGTTGAAGTTGTCGAGGTGGCCAGCGGCGATCATGCTGCAGTCGGCGATGGAGCAACTAGTTTGGATGGGCTGTCTTCAGGAAGGTCGCTAGACCCTTCTCTGGTGGCGGTACTGGGTGCAGATGGCGCAGGGGATCCGGGAAAAACGGCAATCGACCCTGTCCCGGGTGTCCATAATAGTTTGTCCCGGGTGTCCACAACAGTTTGGACCTCTAAGAGATTGGGCTACTGTGGGGCCCACAGTTTGTTGGGCCAGATCTCCCTATGGGCCTGGATGTGGGATCCAGGAGACTTGTGCATCTTATTACGATGCTTTCAAGAGATTAGCCCTAAGAATAAGGGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.78

Weight (kDa)

9.32

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 284
AciI CCGC 4 cut(s) 7, 231, 324, 407
AclWI GGATC 4 cut(s) 428, 441, 575, 588
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 5 cut(s) 25, 400, 463, 464, 576
AgsI TTSAA 4 cut(s) 25, 197, 305, 617
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 2 cut(s) 569, 583
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
Alw26I GTCTC 3 cut(s) 74, 130, 582
AlwI GGATC 4 cut(s) 428, 441, 575, 588
Ama87I CYCGRG 2 cut(s) 463, 486
AoxI GGCC 4 cut(s) 318, 534, 550, 567
ApaI GGGCCC 1 cut(s) 538
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 1 cut(s) 91
AseI ATTAAT 1 cut(s) 213
AspLEI GCGC 2 cut(s) 38, 428
AspS9I GGNCC 5 cut(s) 507, 534, 535, 550, 567
AsuC2I CCSGG 6 cut(s) 260, 438, 464, 465, 487, 488
AsuHPI GGTGA 2 cut(s) 230, 290
AsuII TTCGAA 1 cut(s) 273
AvaI CYCGRG 2 cut(s) 463, 486
AvaII GGWCC 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 320
BamHI GGATCC 2 cut(s) 433, 580
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 322
BccI CCATC 6 cut(s) 80, 243, 282, 341, 359, 416
BceAI ACGGC 1 cut(s) 462
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 2 cut(s) 571, 585
BcnI CCSGG 6 cut(s) 260, 438, 464, 465, 487, 488
BcoDI GTCTC 3 cut(s) 74, 130, 582
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 4 cut(s) 108, 129, 357, 389
BfmI CTRYAG 1 cut(s) 336
BglII AGATCT 1 cut(s) 555
BisI GCNGC 2 cut(s) 325, 336
BlsI GCNGC 2 cut(s) 326, 337
Bme1390I CCNGG 8 cut(s) 260, 438, 464, 465, 487, 488, 571, 585
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 2 cut(s) 463, 486
BmgT120I GGNCC 5 cut(s) 507, 534, 535, 550, 567
BmiI GGNNCC 5 cut(s) 257, 435, 535, 536, 582
BmrFI CCNGG 8 cut(s) 260, 438, 464, 465, 487, 488, 571, 585
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 2 cut(s) 599, 606
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 273
BpuMI CCSGG 6 cut(s) 260, 438, 464, 465, 487, 488
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 463, 486
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 400, 463, 464, 576
Bse1I ACTGG 1 cut(s) 417
BseBI CCWGG 2 cut(s) 571, 585
BseDI CCNNGG 2 cut(s) 463, 486
BseGI GGATG 2 cut(s) 370, 579
BseLI CCNNNNNNNGG 5 cut(s) 25, 400, 463, 464, 576
BseNI ACTGG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 438
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 4 cut(s) 320, 536, 552, 569
BsiHKCI CYCGRG 2 cut(s) 463, 486
BsiSI CCGG 4 cut(s) 259, 437, 464, 487
BslFI GGGAC 2 cut(s) 446, 469
BslI CCNNNNNNNGG 5 cut(s) 25, 400, 463, 464, 576
BsmAI GTCTC 3 cut(s) 74, 130, 582
BsmFI GGGAC 2 cut(s) 446, 469
BsnI GGCC 4 cut(s) 320, 536, 552, 569
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 463, 486
Bsp119I TTCGAA 1 cut(s) 273
Bsp120I GGGCCC 1 cut(s) 534
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 5 cut(s) 169, 328, 433, 555, 580
BspACI CCGC 4 cut(s) 7, 231, 324, 407
BspANI GGCC 4 cut(s) 320, 536, 552, 569
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 5 cut(s) 257, 435, 535, 536, 582
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 4 cut(s) 428, 441, 575, 588
BspT104I TTCGAA 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 463, 486
BssMI GATC 5 cut(s) 169, 328, 433, 555, 580
Bst2UI CCWGG 2 cut(s) 571, 585
Bst4CI ACNGT 3 cut(s) 502, 530, 542
Bst6I CTCTTC 3 cut(s) 72, 94, 160
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 513, 630
BstF5I GGATG 2 cut(s) 370, 579
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 2 cut(s) 38, 428
BstKTI GATC 5 cut(s) 172, 331, 436, 558, 583
BstMAI GTCTC 3 cut(s) 74, 130, 582
BstMBI GATC 5 cut(s) 169, 328, 433, 555, 580
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 2 cut(s) 571, 585
BstSCI CCNGG 8 cut(s) 258, 436, 462, 463, 485, 486, 569, 583
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 3 cut(s) 433, 555, 580
BstYI RGATCY 3 cut(s) 433, 555, 580
BsuRI GGCC 4 cut(s) 320, 536, 552, 569
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 2 cut(s) 370, 579
Cac8I GCNNGC 1 cut(s) 322
CfoI GCGC 2 cut(s) 38, 428
Cfr13I GGNCC 5 cut(s) 507, 534, 535, 550, 567
Cfr9I CCCGGG 2 cut(s) 463, 486
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 410
CviAII CATG 2 cut(s) 177, 332
CviJI RGCY 9 cut(s) 18, 208, 320, 370, 525, 536, 552, 569, 627
CviKI_1 RGCY 9 cut(s) 18, 208, 320, 370, 525, 536, 552, 569, 627
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 2 cut(s) 513, 630
DpnI GATC 5 cut(s) 171, 330, 435, 557, 582
DpnII GATC 5 cut(s) 169, 328, 433, 555, 580
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 3 cut(s) 72, 94, 160
EarI CTCTTC 3 cut(s) 72, 94, 160
EciI GGCGGA 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 2 cut(s) 463, 486
EcoO109I RGGNCCY 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 569, 583
EcoT38I GRGCYC 1 cut(s) 538
FaeI CATG 2 cut(s) 180, 335
FaiI YATR 5 cut(s) 117, 178, 333, 474, 565
FaqI GGGAC 2 cut(s) 446, 469
FatI CATG 2 cut(s) 176, 331
Fnu4HI GCNGC 2 cut(s) 325, 336
FokI GGATG 2 cut(s) 377, 586
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 2 cut(s) 325, 336
FspBI CTAG 4 cut(s) 108, 129, 357, 389
GlaI GCGC 2 cut(s) 37, 427
GluI GCNGC 2 cut(s) 325, 336
HaeIII GGCC 4 cut(s) 320, 536, 552, 569
HapII CCGG 4 cut(s) 259, 437, 464, 487
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 38, 428
Hin1II CATG 2 cut(s) 180, 335
Hin6I GCGC 2 cut(s) 36, 426
HinP1I GCGC 2 cut(s) 36, 426
HinfI GANTC 1 cut(s) 160
HpaII CCGG 4 cut(s) 259, 437, 464, 487
HphI GGTGA 2 cut(s) 230, 290
Hpy166II GTNNAC 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 378, 617
Hpy8I GTNNAC 1 cut(s) 495
HpyAV CCTTC 6 cut(s) 65, 146, 198, 269, 375, 405
HpyCH4III ACNGT 3 cut(s) 502, 530, 542
HpyCH4V TGCA 3 cut(s) 338, 419, 597
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 2 cut(s) 513, 630
Hsp92II CATG 2 cut(s) 180, 335
HspAI GCGC 2 cut(s) 36, 426
Kzo9I GATC 5 cut(s) 169, 328, 433, 555, 580
LmnI GCTCC 1 cut(s) 350
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 599, 606
MaeI CTAG 4 cut(s) 108, 129, 357, 389
MaeIII GTNAC 1 cut(s) 278
MalI GATC 5 cut(s) 171, 330, 435, 557, 582
MboI GATC 5 cut(s) 169, 328, 433, 555, 580
MboII GAAGA 6 cut(s) 58, 61, 89, 111, 177, 366
MflI RGATCY 3 cut(s) 433, 555, 580
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 1 cut(s) 91
MluNI TGGCCA 1 cut(s) 320
MnlI CCTC 6 cut(s) 95, 115, 139, 232, 307, 520
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 324
MspI CCGG 4 cut(s) 259, 437, 464, 487
MspR9I CCNGG 8 cut(s) 260, 438, 464, 465, 487, 488, 571, 585
MvaI CCWGG 2 cut(s) 571, 585
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 425
NciI CCSGG 6 cut(s) 260, 438, 464, 465, 487, 488
NdeII GATC 5 cut(s) 169, 328, 433, 555, 580
NlaIII CATG 2 cut(s) 180, 335
NlaIV GGNNCC 5 cut(s) 257, 435, 535, 536, 582
NmuCI GTSAC 1 cut(s) 278
NspV TTCGAA 1 cut(s) 273
PfeI GAWTC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 458
PfoI TCCNGGA 2 cut(s) 436, 583
PkrI GCNGC 2 cut(s) 326, 337
PshBI ATTAAT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 569, 583
PspGI CCWGG 2 cut(s) 569, 583
PspN4I GGNNCC 5 cut(s) 257, 435, 535, 536, 582
PspOMI GGGCCC 1 cut(s) 534
PspPI GGNCC 5 cut(s) 507, 534, 535, 550, 567
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 3 cut(s) 433, 555, 580
PsyI GACNNNGTC 1 cut(s) 458
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 325, 336
Sau3AI GATC 5 cut(s) 169, 328, 433, 555, 580
Sau96I GGNCC 5 cut(s) 507, 534, 535, 550, 567
ScrFI CCNGG 8 cut(s) 260, 438, 464, 465, 487, 488, 571, 585
SduI GDGCHC 1 cut(s) 538
SetI ASST 5 cut(s) 126, 280, 318, 386, 512
SfaNI GCATC 2 cut(s) 599, 606
SfcI CTRYAG 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 273
SinI GGWCC 1 cut(s) 507
SmaI CCCGGG 2 cut(s) 465, 488
SpeI ACTAGT 1 cut(s) 356
Sse9I AATT 1 cut(s) 91
SsiI CCGC 4 cut(s) 7, 231, 324, 407
SspMI CTAG 4 cut(s) 108, 129, 357, 389
StyD4I CCNGG 8 cut(s) 258, 436, 462, 463, 485, 486, 569, 583
TaaI ACNGT 3 cut(s) 502, 530, 542
TaqI TCGA 5 cut(s) 163, 247, 273, 312, 453
TasI AATT 1 cut(s) 91
TauI GCSGC 1 cut(s) 327
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 2 cut(s) 463, 486
Tth111I GACNNNGTC 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 507
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 1 cut(s) 91
XmaI CCCGGG 2 cut(s) 463, 486
XspI CTAG 4 cut(s) 108, 129, 357, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.