RLG00000021581

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016970
Physical Location & Seq
Reverse (-)
76578015 .. 76580996
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021581

Sequence Viewer

Length: 612 bp
ATGGACCATCCTTTCGCGAAAACATTTCTCGTCAACCTCACTCCACAAACTCAAGAGCGAATCCTAGAACGATGTGTTTGTATCATGCCAGTGCTGGACAAGATAATCGTCATGCATAAAGCCCCAAGTGTACCGTTTCTTTTGAAAGCTTTTCTACTCGCTCTAGCTCTTCTTCACAGGAATGGTGGGTTGCCCTTGTGGCAATGCTCGGCTATTGGTTCCGCTGCAGAGTACGACCATGGTGGGGATATTTTTGTGATCAGGAGAGGAGATCTAGGTTCGGGCTCGAGCGGCATCCTTTTGCGTCTAGGGGTTGGCGGCTTTTCGATGGTGAAACCAGCTACTGATTGCGGCGGTGCAGTGGTGGTTGGCAGTAGCTGGTTTGCCCTTGATTTGGATAGTACTGATGTTGTCGATGTCGAAGTTGGCATGGTGGCCAGCAATGGTGGTCATGCTGCAGTCGGCGCCAGAGCTGCTGGCTTGGATGGTCTGTTTTCAGCAAGGCTGCTAGAGCTTCTTCTGGTGGCGGTGCTAGGTGCAAATGGAGCAAGGGAAGGGAGAAGGACAGTGGTCGACCCAAATCTAGGTGTCCACTACAGGTCGAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

21.3

Weight (kDa)

6.58

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 1 cut(s) 573
AccII CGCG 1 cut(s) 17
AciI CCGC 6 cut(s) 222, 291, 318, 351, 354, 527
AcoI YGGCCR 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 3 cut(s) 132, 233, 403
AfiI CCNNNNNNNGG 3 cut(s) 244, 394, 584
AgsI TTSAA 1 cut(s) 145
AluBI AGCT 7 cut(s) 149, 167, 341, 378, 473, 514, 606
AluI AGCT 7 cut(s) 149, 167, 341, 378, 473, 514, 606
AlwNI CAGNNNCTG 2 cut(s) 344, 378
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 4 cut(s) 224, 455, 473, 505
ArsI GACNNNNNNTTYG 1 cut(s) 28
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 287
BbvI GCAGC 4 cut(s) 211, 442, 460, 492
BccI CCATC 3 cut(s) 15, 322, 479
BclI TGATCA 1 cut(s) 258
BfaI CTAG 7 cut(s) 65, 164, 275, 308, 509, 533, 584
BfmI CTRYAG 3 cut(s) 225, 456, 595
BfoI RGCGCY 1 cut(s) 468
BglI GCCNNNNNGGC 1 cut(s) 199
BglII AGATCT 1 cut(s) 271
BisI GCNGC 7 cut(s) 225, 292, 319, 352, 456, 474, 506
BlsI GCNGC 7 cut(s) 226, 293, 320, 353, 457, 475, 507
BmcAI AGTACT 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 220, 466
BmsI GCATC 1 cut(s) 303
BoxI GACNNNNGTC 1 cut(s) 569
BpuEI CTTGAG 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 238
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 394, 584
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 2 cut(s) 209, 448
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 3 cut(s) 7, 294, 490
BseLI CCNNNNNNNGG 3 cut(s) 244, 394, 584
BseMI GCAATG 2 cut(s) 209, 448
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 4 cut(s) 211, 442, 460, 492
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 437
BshNI GGYRCC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 286
BslI CCNNNNNNNGG 3 cut(s) 244, 394, 584
BsnI GGCC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 2 cut(s) 258, 271
Bsp19I CCATGG 1 cut(s) 238
Bsp68I TCGCGA 1 cut(s) 17
BspACI CCGC 6 cut(s) 222, 291, 318, 351, 354, 527
BspANI GGCC 1 cut(s) 437
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 220, 466
BspMAI CTGCAG 2 cut(s) 229, 460
BspQI GCTCTTC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 291
BsrDI GCAATG 2 cut(s) 209, 448
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 2 cut(s) 258, 271
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 135, 568
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 439, 478
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 7, 294, 490
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 2 cut(s) 261, 274
BstMBI GATC 2 cut(s) 258, 271
BstMWI GCNNNNNNNGC 6 cut(s) 199, 291, 464, 473, 511, 545
BstPAI GACNNNNGTC 1 cut(s) 569
BstSFI CTRYAG 3 cut(s) 225, 456, 595
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 211, 442, 460, 492
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 7, 294, 490
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 96, 366, 573
BtuMI TCGCGA 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 439, 478
CaiI CAGNNNCTG 2 cut(s) 344, 378
CfoI GCGC 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 293
Csp6I GTAC 3 cut(s) 131, 232, 402
CviAII CATG 5 cut(s) 85, 112, 239, 430, 452
CviQI GTAC 3 cut(s) 131, 232, 402
DinI GGCGCC 1 cut(s) 466
DpnI GATC 2 cut(s) 260, 273
DpnII GATC 2 cut(s) 258, 271
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 287
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 5 cut(s) 88, 115, 242, 433, 455
FaiI YATR 6 cut(s) 86, 113, 117, 240, 431, 453
FatI CATG 5 cut(s) 84, 111, 238, 429, 451
FbaI TGATCA 1 cut(s) 258
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 7 cut(s) 225, 292, 319, 352, 456, 474, 506
FokI GGATG 2 cut(s) 281, 497
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 7 cut(s) 225, 292, 319, 352, 456, 474, 506
FspBI CTAG 7 cut(s) 65, 164, 275, 308, 509, 533, 584
GlaI GCGC 1 cut(s) 466
GluI GCNGC 7 cut(s) 225, 292, 319, 352, 456, 474, 506
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 1 cut(s) 437
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 5 cut(s) 88, 115, 242, 433, 455
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HincII GTYRAC 2 cut(s) 34, 574
HindII GTYRAC 2 cut(s) 34, 574
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 1 cut(s) 60
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 4 cut(s) 34, 131, 574, 592
Hpy188III TCNNGA 3 cut(s) 16, 53, 262
Hpy8I GTNNAC 4 cut(s) 34, 131, 574, 592
HpyAV CCTTC 2 cut(s) 548, 555
HpyCH4III ACNGT 2 cut(s) 135, 568
HpyCH4V TGCA 5 cut(s) 115, 227, 359, 458, 539
HpyF10VI GCNNNNNNNGC 6 cut(s) 199, 291, 464, 473, 511, 545
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 5 cut(s) 88, 115, 242, 433, 455
HspAI GCGC 1 cut(s) 465
KasI GGCGCC 1 cut(s) 464
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 2 cut(s) 258, 271
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 4 cut(s) 211, 442, 460, 492
LweI GCATC 1 cut(s) 303
MaeI CTAG 7 cut(s) 65, 164, 275, 308, 509, 533, 584
MalI GATC 2 cut(s) 260, 273
MbiI CCGCTC 1 cut(s) 291
MboI GATC 2 cut(s) 258, 271
MboII GAAGA 3 cut(s) 161, 164, 509
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 287
MlsI TGGCCA 1 cut(s) 437
MluNI TGGCCA 1 cut(s) 437
Mly113I GGCGCC 1 cut(s) 465
MnlI CCTC 2 cut(s) 47, 260
Mox20I TGGCCA 1 cut(s) 437
Mph1103I ATGCAT 1 cut(s) 117
MscI TGGCCA 1 cut(s) 437
MslI CAYNNNNRTG 2 cut(s) 89, 180
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 224
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 6 cut(s) 199, 291, 464, 473, 511, 545
NarI GGCGCC 1 cut(s) 465
NcoI CCATGG 1 cut(s) 238
NdeII GATC 2 cut(s) 258, 271
NlaIII CATG 5 cut(s) 88, 115, 242, 433, 455
NlaIV GGNNCC 2 cut(s) 220, 466
NmeAIII GCCGAG 1 cut(s) 188
NruI TCGCGA 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 60
PkrI GCNGC 7 cut(s) 226, 293, 320, 353, 457, 475, 507
PluTI GGCGCC 1 cut(s) 468
PshAI GACNNNNGTC 1 cut(s) 569
PspN4I GGNNCC 2 cut(s) 220, 466
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 286
PstI CTGCAG 2 cut(s) 229, 460
PstNI CAGNNNCTG 2 cut(s) 344, 378
PsuI RGATCY 1 cut(s) 271
RruI TCGCGA 1 cut(s) 17
RsaI GTAC 3 cut(s) 132, 233, 403
RsaNI GTAC 3 cut(s) 131, 232, 402
RseI CAYNNNNRTG 2 cut(s) 89, 180
SalI GTCGAC 1 cut(s) 572
SapI GCTCTTC 1 cut(s) 174
SatI GCNGC 7 cut(s) 225, 292, 319, 352, 456, 474, 506
Sau3AI GATC 2 cut(s) 258, 271
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 403
SduI GDGCHC 1 cut(s) 287
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 3 cut(s) 225, 456, 595
SfoI GGCGCC 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 286
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 89, 180
SmlI CTYRAG 2 cut(s) 51, 286
SmoI CTYRAG 2 cut(s) 51, 286
SsiI CCGC 6 cut(s) 222, 291, 318, 351, 354, 527
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 7 cut(s) 65, 164, 275, 308, 509, 533, 584
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 135, 568
TaqI TCGA 6 cut(s) 287, 326, 414, 420, 573, 602
TatI WGTACW 1 cut(s) 401
TauI GCSGC 3 cut(s) 294, 321, 354
TfiI GAWTC 1 cut(s) 60
TscAI CASTG 3 cut(s) 96, 366, 573
TseI GCWGC 4 cut(s) 224, 455, 473, 505
TspRI CASTG 3 cut(s) 96, 366, 573
VpaK11BI GGWCC 1 cut(s) 4
XhoI CTCGAG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 7 cut(s) 65, 164, 275, 308, 509, 533, 584
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.