RchiOBHm_Chr6g0295241

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008121
Physical Location & Seq
Forward (+)
57324670 .. 57329313
4644 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26494

Sequence Viewer

Length: 1047 bp
ATGCACATTGCACTACTTTGCTTAGTAATTGTCCTTGCAAAATTAATGGACGTAGAGAGAGTCCAAGCCTTGGCCTTGCGTGGGCTCAACGAGCTCCCAGCGAAGTTCATCCGCCCGACTCATGAGCAACCCGAGAACAGCAAGGCCTTGGAGGGAGTCACCGTGCCGGTGATCTCCCTGGCTAAACCACATGACATTGTCGTGAAGGAAGTGGCTGAGGCAGCCTCCGAGTGGGGCTTCTTTCTCATCACTGACCATGGCATACCAGCTTCTTTGATAGAGCAGTTACAGAAGGTGGGCAAAGAGTTCTTTATGCTGCCACAGGAGGAAAAAGAGGCTTATGCAAATGACCCTGCTAATGGCAAATTTGATGGGTATGGAACCACGATGACTAAGAACCTTGATGAAAAGGTTGAGTGGATTGACTATTTCTTTCACGTCACTGCTCCTCCATATAAGGTCAACGATGAAATCTGGCCTAGGAATCCTCCATCATACAGGGAAGTAAATGAAGAATACAGTAGGGAAATGCTGAGAGTAACAGACAAGCTACTGGAGTTGCTTTCCGAGAGTCTAGGCCTGGACAAGCAGGTACTAAAATCACATGTCGGAGGTGACAAAATAGAGCTGGAAATGAAGATAAACATGTACCCGCCATGCCCACAGCCGCAACTGGCTCTCGGAGTTGAGCCTCACACAGACATGTCGGCCCTCACCTTGCTGGTTTCGAACGATGTTCCTGGTCTTCAGCTGTGGAAAGATGGTAACTGGGTTTCAGTAAATTACCTAACCAAGACAGGAGCAATCTTCGTTCACATTGGTGATCAGATGGAGGTGTTGAGCAATGGCAAGTACAAGAGTGTTCTTCACAGGAGCTTGGTGAACAAGGAACGGTTGCGCATGTCGTGGGCTGTGTTCGTTGTGCCGCCGCATGAGGCGGTCATTGGGCCTCTGCCAGAGCTTTTGGATGAACAAAACCCTGCCAAATACTCTACCAAAACATATGCTGAATACCGGCACCGCAAATTCAACAAAATCCCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

348

Amino Acids

39.48

Weight (kDa)

5.72

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 160 7e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 308 6.4e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 1017
AccB7I CCANNNNNTGG 1 cut(s) 70
AciI CCGC 7 cut(s) 112, 653, 668, 926, 929, 938, 1021
AcsI RAATTY 2 cut(s) 365, 1025
AcuI CTGAAG 1 cut(s) 731
AfaI GTAC 3 cut(s) 594, 650, 854
AfiI CCNNNNNNNGG 4 cut(s) 70, 81, 231, 359
AflIII ACRYGT 3 cut(s) 604, 645, 702
AgsI TTSAA 1 cut(s) 1030
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 3 cut(s) 177, 579, 739
AleI CACNNNNGTG 1 cut(s) 819
AluBI AGCT 7 cut(s) 94, 269, 550, 628, 751, 876, 961
AluI AGCT 7 cut(s) 94, 269, 550, 628, 751, 876, 961
Alw21I GWGCWC 1 cut(s) 96
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 6 cut(s) 72, 144, 476, 577, 708, 947
ApeKI GCWGC 2 cut(s) 221, 316
ApoI RAATTY 2 cut(s) 365, 1025
AseI ATTAAT 1 cut(s) 44
AspA2I CCTAGG 1 cut(s) 479
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 2 cut(s) 709, 947
AsuHPI GGTGA 6 cut(s) 151, 181, 626, 706, 833, 892
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 1 cut(s) 131
AvrII CCTAGG 1 cut(s) 479
BanI GGYRCC 1 cut(s) 1017
BanII GRGCYC 2 cut(s) 87, 96
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 233, 303
BccI CCATC 4 cut(s) 365, 499, 755, 823
BciT130I CCWGG 3 cut(s) 179, 581, 741
BclI TGATCA 1 cut(s) 823
BfaI CTAG 2 cut(s) 480, 575
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 5 cut(s) 222, 317, 668, 926, 929
BlnI CCTAGG 1 cut(s) 479
BlsI GCNGC 5 cut(s) 223, 318, 669, 927, 930
Bme1390I CCNGG 3 cut(s) 179, 581, 741
BmeT110I CYCGRG 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 709, 947
BmiI GGNNCC 2 cut(s) 382, 1019
BmrFI CCNGG 3 cut(s) 179, 581, 741
BmrI ACTGGG 1 cut(s) 778
BmuI ACTGGG 1 cut(s) 778
BpiI GAAGAC 1 cut(s) 737
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 728
BsaJI CCNNGG 5 cut(s) 69, 147, 177, 256, 479
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 433, 463
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 81, 231, 359
Bse118I RCCGGY 2 cut(s) 166, 1014
Bse1I ACTGG 3 cut(s) 558, 678, 773
Bse3DI GCAATG 2 cut(s) 6, 850
BseBI CCWGG 3 cut(s) 179, 581, 741
BseDI CCNNGG 5 cut(s) 69, 147, 177, 256, 479
BseGI GGATG 2 cut(s) 108, 973
BseLI CCNNNNNNNGG 4 cut(s) 70, 81, 231, 359
BseMI GCAATG 2 cut(s) 6, 850
BseMII CTCAG 2 cut(s) 207, 524
BseNI ACTGG 3 cut(s) 558, 678, 773
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 233, 303
BseYI CCCAGC 1 cut(s) 97
BshFI GGCC 6 cut(s) 74, 146, 478, 579, 710, 949
BshNI GGYRCC 1 cut(s) 1017
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 2 cut(s) 167, 1015
BslI CCNNNNNNNGG 4 cut(s) 70, 81, 231, 359
BsnI GGCC 6 cut(s) 74, 146, 478, 579, 710, 949
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 87, 96
Bsp143I GATC 2 cut(s) 171, 823
Bsp19I CCATGG 1 cut(s) 256
BspACI CCGC 7 cut(s) 112, 653, 668, 926, 929, 938, 1021
BspANI GGCC 6 cut(s) 74, 146, 478, 579, 710, 949
BspCNI CTCAG 2 cut(s) 208, 525
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 382, 1019
BspMI ACCTGC 1 cut(s) 580
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 1017
BsrDI GCAATG 2 cut(s) 6, 850
BsrFI RCCGGY 2 cut(s) 166, 1014
BsrI ACTGG 3 cut(s) 558, 678, 773
BssAI RCCGGY 2 cut(s) 166, 1014
BssECI CCNNGG 5 cut(s) 69, 147, 177, 256, 479
BssMI GATC 2 cut(s) 171, 823
BssT1I CCWWGG 4 cut(s) 69, 147, 256, 479
Bst2UI CCWGG 3 cut(s) 179, 581, 741
Bst4CI ACNGT 4 cut(s) 163, 521, 894, 1044
BstBI TTCGAA 1 cut(s) 728
BstDEI CTNAG 4 cut(s) 22, 216, 393, 533
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 108, 973
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 2 cut(s) 174, 826
BstMBI GATC 2 cut(s) 171, 823
BstMWI GCNNNNNNNGC 2 cut(s) 91, 221
BstNI CCWGG 3 cut(s) 179, 581, 741
BstNSI RCATGY 4 cut(s) 608, 649, 706, 904
BstSCI CCNGG 3 cut(s) 177, 579, 739
BstV1I GCAGC 2 cut(s) 233, 303
BstV2I GAAGAC 1 cut(s) 737
BsuRI GGCC 6 cut(s) 74, 146, 478, 579, 710, 949
BtgI CCRYGG 1 cut(s) 256
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 108, 973
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 249, 441
BveI ACCTGC 1 cut(s) 580
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 900
Cfr10I RCCGGY 2 cut(s) 166, 1014
Cfr13I GGNCC 2 cut(s) 709, 947
Csp6I GTAC 3 cut(s) 593, 649, 853
CviAII CATG 9 cut(s) 122, 191, 257, 605, 646, 657, 703, 901, 932
CviQI GTAC 3 cut(s) 593, 649, 853
DdeI CTNAG 4 cut(s) 22, 216, 393, 533
DpnI GATC 2 cut(s) 173, 825
DpnII GATC 2 cut(s) 171, 823
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 69, 147, 256, 479
Eco147I AGGCCT 2 cut(s) 146, 579
Eco24I GRGCYC 2 cut(s) 87, 96
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 731
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 3 cut(s) 177, 579, 739
EcoT14I CCWWGG 4 cut(s) 69, 147, 256, 479
EcoT38I GRGCYC 2 cut(s) 87, 96
ErhI CCWWGG 4 cut(s) 69, 147, 256, 479
FaeI CATG 9 cut(s) 125, 194, 260, 608, 649, 660, 706, 904, 935
FatI CATG 9 cut(s) 121, 190, 256, 604, 645, 656, 702, 900, 931
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 5 cut(s) 222, 317, 668, 926, 929
FokI GGATG 2 cut(s) 95, 980
FriOI GRGCYC 2 cut(s) 87, 96
Fsp4HI GCNGC 5 cut(s) 222, 317, 668, 926, 929
FspBI CTAG 2 cut(s) 480, 575
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 1 cut(s) 899
GluI GCNGC 5 cut(s) 222, 317, 668, 926, 929
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 575
HaeIII GGCC 6 cut(s) 74, 146, 478, 579, 710, 949
HapII CCGG 2 cut(s) 167, 1015
HhaI GCGC 1 cut(s) 900
Hin1II CATG 9 cut(s) 125, 194, 260, 608, 649, 660, 706, 904, 935
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 5 cut(s) 60, 118, 156, 484, 571
HpaII CCGG 2 cut(s) 167, 1015
HphI GGTGA 6 cut(s) 151, 181, 626, 706, 833, 892
Hpy166II GTNNAC 3 cut(s) 463, 814, 883
Hpy188I TCNGA 5 cut(s) 229, 568, 611, 683, 828
Hpy188III TCNNGA 2 cut(s) 122, 202
Hpy8I GTNNAC 3 cut(s) 463, 814, 883
HpyAV CCTTC 2 cut(s) 199, 286
HpyCH4III ACNGT 4 cut(s) 163, 521, 894, 1044
HpyCH4IV ACGT 2 cut(s) 51, 438
HpyCH4V TGCA 4 cut(s) 4, 11, 38, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 221
HpyF3I CTNAG 4 cut(s) 22, 216, 393, 533
HpySE526I ACGT 2 cut(s) 51, 438
Hsp92II CATG 9 cut(s) 125, 194, 260, 608, 649, 660, 706, 904, 935
HspAI GCGC 1 cut(s) 898
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 2 cut(s) 171, 823
LmnI GCTCC 4 cut(s) 99, 451, 800, 873
Lsp1109I GCAGC 2 cut(s) 233, 303
MaeI CTAG 2 cut(s) 480, 575
MaeII ACGT 2 cut(s) 51, 438
MaeIII GTNAC 6 cut(s) 157, 285, 439, 538, 614, 764
MalI GATC 2 cut(s) 173, 825
MboI GATC 2 cut(s) 171, 823
MboII GAAGA 5 cut(s) 524, 649, 737, 799, 857
MhlI GDGCHC 2 cut(s) 87, 96
MluCI AATT 5 cut(s) 27, 41, 365, 781, 1025
MlyI GAGTC 4 cut(s) 69, 112, 165, 580
MmeI TCCRAC 1 cut(s) 589
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 3 cut(s) 200, 701, 819
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 2 cut(s) 167, 1015
MspR9I CCNGG 3 cut(s) 179, 581, 741
MvaI CCWGG 3 cut(s) 179, 581, 741
MwoI GCNNNNNNNGC 2 cut(s) 91, 221
NcoI CCATGG 1 cut(s) 256
NdeI CATATG 1 cut(s) 1003
NdeII GATC 2 cut(s) 171, 823
NlaIII CATG 9 cut(s) 125, 194, 260, 608, 649, 660, 706, 904, 935
NlaIV GGNNCC 2 cut(s) 382, 1019
NmuCI GTSAC 3 cut(s) 157, 439, 614
NsbI TGCGCA 1 cut(s) 899
NspI RCATGY 4 cut(s) 608, 649, 706, 904
NspV TTCGAA 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 819
PagI TCATGA 1 cut(s) 121
PceI AGGCCT 2 cut(s) 146, 579
PciI ACATGT 3 cut(s) 604, 645, 702
PfeI GAWTC 1 cut(s) 484
PflFI GACNNNGTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 70
PkrI GCNGC 5 cut(s) 223, 318, 669, 927, 930
PleI GAGTC 4 cut(s) 68, 112, 164, 579
PpsI GAGTC 4 cut(s) 68, 112, 164, 579
PscI ACATGT 3 cut(s) 604, 645, 702
PshBI ATTAAT 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 3 cut(s) 177, 579, 739
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 3 cut(s) 177, 579, 739
PspN4I GGNNCC 2 cut(s) 382, 1019
PspPI GGNCC 2 cut(s) 709, 947
PsyI GACNNNGTC 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 3 cut(s) 594, 650, 854
RsaNI GTAC 3 cut(s) 593, 649, 853
RseI CAYNNNNRTG 3 cut(s) 200, 701, 819
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 5 cut(s) 222, 317, 668, 926, 929
Sau3AI GATC 2 cut(s) 171, 823
Sau96I GGNCC 2 cut(s) 709, 947
SchI GAGTC 4 cut(s) 69, 112, 165, 580
ScrFI CCNGG 3 cut(s) 179, 581, 741
SduI GDGCHC 2 cut(s) 87, 96
SfuI TTCGAA 1 cut(s) 728
SmiMI CAYNNNNRTG 3 cut(s) 200, 701, 819
Sse9I AATT 5 cut(s) 27, 41, 365, 781, 1025
SseBI AGGCCT 2 cut(s) 146, 579
SsiI CCGC 7 cut(s) 112, 653, 668, 926, 929, 938, 1021
SspMI CTAG 2 cut(s) 480, 575
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 2 cut(s) 146, 579
StyD4I CCNGG 3 cut(s) 177, 579, 739
StyI CCWWGG 4 cut(s) 69, 147, 256, 479
TaaI ACNGT 4 cut(s) 163, 521, 894, 1044
TaiI ACGT 2 cut(s) 54, 441
TaqI TCGA 1 cut(s) 728
TasI AATT 5 cut(s) 27, 41, 365, 781, 1025
TatI WGTACW 1 cut(s) 852
TauI GCSGC 3 cut(s) 670, 928, 931
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 2 cut(s) 256, 448
TseFI GTSAC 3 cut(s) 157, 439, 614
TseI GCWGC 2 cut(s) 221, 316
Tsp45I GTSAC 3 cut(s) 157, 439, 614
TspDTI ATGAA 6 cut(s) 97, 420, 483, 525, 650, 984
TspRI CASTG 2 cut(s) 256, 448
Tth111I GACNNNGTC 1 cut(s) 197
Van91I CCANNNNNTGG 1 cut(s) 70
VspI ATTAAT 1 cut(s) 44
XapI RAATTY 2 cut(s) 365, 1025
XceI RCATGY 4 cut(s) 608, 649, 706, 904
XmaJI CCTAGG 1 cut(s) 479
XspI CTAG 2 cut(s) 480, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.