Rorug06G0249700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008121
Physical Location & Seq
Forward (+)
39575237 .. 39575698
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0249700.1

Sequence Viewer

Length: 462 bp
ATGGCTCCCCAGTTCATGTTTCTCATTGGCAAACCTCCCGTTGTCATCATTCTTGTTCCTTTCTTGTGTATCTTAGCCTCTGTCATTGCCATGTTCACTCGGCCAATTCTATGGCTTGCACGGTTACTACTTAACAGCCGAGGCCTCGAGGACAGGATTCTCCGGTCATTTCCAAAGATCTGTTATACTGCAGAAATCGATGAAAAGCTCACAGAATGCGCTGTCTGCTTGATGGAGTATGAGGATGGAGATGCATTAAGGATTTTGCCTCCTTGTGGTCATGGTTTCCATGTTGACTGTGTTGATAGGTGGCTGAAGTGCCACTCTTCATGTCCATCGTGTCGCGCCGTTGTAGTTATGGAGAGCAAAGCACAGATACTTGGAGACGCCAATAGTCCACCATTCTCAGATACATCATATACAGTTTCAAATCCGTACAGACTGAGTTTCTGGCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

153

Amino Acids

17.26

Weight (kDa)

6.4

Isoelectric Point (pI)

59.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 72 - 115 8.5e-15 Ring finger domain
zf-RING_11 PF17123 72 - 101 2.7e-12 RING-like zinc finger
zf-C3HC4_2 PF13923 72 - 114 8.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 73 - 115 5.1e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 73 - 114 7.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 146
AccII CGCG 1 cut(s) 345
AcoI YGGCCR 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 1 cut(s) 429
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 378
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 2 cut(s) 101, 142
AspLEI GCGC 2 cut(s) 221, 347
AvaI CYCGRG 1 cut(s) 146
BccI CCATC 3 cut(s) 226, 239, 343
BceAI ACGGC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 189
BglII AGATCT 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 4
Bsa29I ATCGAT 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 84
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 2 cut(s) 420, 434
BseNI ACTGG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 2 cut(s) 103, 144
BshVI ATCGAT 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 378
BsmBI CGTCTC 1 cut(s) 378
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 2 cut(s) 103, 144
BsoBI CYCGRG 1 cut(s) 146
Bsp143I GATC 1 cut(s) 177
BspANI GGCC 2 cut(s) 103, 144
BspCNI CTCAG 2 cut(s) 419, 435
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 3 cut(s) 123, 299, 424
Bst6I CTCTTC 1 cut(s) 331
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 3 cut(s) 73, 406, 443
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 2 cut(s) 221, 347
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 2 cut(s) 103, 144
BsuTUI ATCGAT 1 cut(s) 198
BtsCI GGATG 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 117
CfoI GCGC 2 cut(s) 221, 347
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 395
Csp6I GTAC 1 cut(s) 436
CviAII CATG 5 cut(s) 16, 91, 281, 290, 330
CviJI RGCY 8 cut(s) 5, 77, 103, 115, 138, 144, 208, 313
CviKI_1 RGCY 8 cut(s) 5, 77, 103, 115, 138, 144, 208, 313
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 3 cut(s) 73, 406, 443
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 331
Eco147I AGGCCT 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 5 cut(s) 19, 94, 284, 293, 333
FatI CATG 5 cut(s) 15, 90, 280, 289, 329
FokI GGATG 1 cut(s) 257
GlaI GCGC 2 cut(s) 220, 346
HaeIII GGCC 2 cut(s) 103, 144
HapII CCGG 1 cut(s) 163
HgaI GACGC 1 cut(s) 395
HhaI GCGC 2 cut(s) 221, 347
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 5 cut(s) 19, 94, 284, 293, 333
Hin6I GCGC 2 cut(s) 219, 345
HinP1I GCGC 2 cut(s) 219, 345
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 157
HpaII CCGG 1 cut(s) 163
Hpy166II GTNNAC 3 cut(s) 96, 295, 398
Hpy188I TCNGA 1 cut(s) 409
Hpy8I GTNNAC 3 cut(s) 96, 295, 398
HpyCH4III ACNGT 3 cut(s) 123, 299, 424
HpyCH4V TGCA 3 cut(s) 119, 191, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 3 cut(s) 73, 406, 443
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 5 cut(s) 19, 94, 284, 293, 333
HspAI GCGC 2 cut(s) 219, 345
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 4 cut(s) 23, 139, 176, 436
LweI GCATC 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 123
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 1 cut(s) 318
MflI RGATCY 1 cut(s) 177
MluCI AATT 1 cut(s) 105
MnlI CCTC 7 cut(s) 45, 88, 134, 142, 155, 235, 279
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 132, 257
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 221
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeII GATC 1 cut(s) 177
NlaIII CATG 5 cut(s) 19, 94, 284, 293, 333
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 79, 164
NsiI ATGCAT 1 cut(s) 256
PaeR7I CTCGAG 1 cut(s) 146
PceI AGGCCT 1 cut(s) 144
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 146
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 2 cut(s) 132, 257
Sau3AI GATC 1 cut(s) 177
SetI ASST 3 cut(s) 37, 210, 311
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 144
StuI AGGCCT 1 cut(s) 144
TaaI ACNGT 3 cut(s) 123, 299, 424
TaqI TCGA 2 cut(s) 147, 198
TasI AATT 1 cut(s) 105
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 132, 257
Tru9I TTAA 2 cut(s) 132, 257
TspDTI ATGAA 3 cut(s) 4, 216, 318
TspGWI ACGGA 1 cut(s) 423
XhoI CTCGAG 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.