RchiOBHm_Chr7g0193871

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012561
Physical Location & Seq
Forward (+)
12149331 .. 12149819
489 bp
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UTR
Exon/CDS
Intron
PRQ17338

Sequence Viewer

Length: 489 bp
ATGCTTGAACTCAACTCTCAACTGAAGCAGCTAGACAAAATTTGCAATGTTAAAGGCGCCTGGAAGATGTTCAATCAAAGGCTTAAGAGAGATGCGGTTTCATGGACCAGTATGATTTCAGGCTATGTTAGAGTCTCTGATTTCTCTAAAGCATTAGCTCTGTTCTCTAACATGTGGGTACAACCAGGTCTCTGCATGGACCCTTTTCTTCTTAGCATTGCACTCAGGGCTTGTGGACTTGGTATTAACTTGTCTTATGGGGAAGCTGTACATGGGTACTTGGCGAAATCGGGTATTGTGAACTCTATCTTTGTGGGAAGTGCTCTTCTTGACTTGTATATGAAGGTTGTGGGGATTGAGAAGGGTTGTATAATTTTCGACAAAATGCCGCTAAGAAATGTGGTGTCTGGGACTACCATTATCACTGGTGAAATTGGTGAGCAAGTTCAAATTGTTGGTGATGATCTTCTTGTTACAAAACCAAGGTGA

Protein Analysis

162

Amino Acids

17.8

Weight (kDa)

8.93

Isoelectric Point (pI)

22.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 33 - 58 4.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 2 cut(s) 95, 389
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 3 cut(s) 180, 270, 278
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 3 cut(s) 8, 73, 449
AjnI CCWGG 2 cut(s) 59, 184
AluBI AGCT 3 cut(s) 31, 158, 266
AluI AGCT 3 cut(s) 31, 158, 266
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 2 cut(s) 139, 194
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 2 cut(s) 105, 199
AsuHPI GGTGA 3 cut(s) 440, 449, 470
AvaII GGWCC 2 cut(s) 105, 199
BanI GGYRCC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 1 cut(s) 40
BciT130I CCWGG 2 cut(s) 61, 186
BcoDI GTCTC 2 cut(s) 139, 194
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 29, 389
BlsI GCNGC 2 cut(s) 30, 390
Bme1390I CCNGG 2 cut(s) 61, 186
Bme18I GGWCC 2 cut(s) 105, 199
BmgT120I GGNCC 2 cut(s) 105, 199
BmiI GGNNCC 2 cut(s) 58, 201
BmrFI CCNGG 2 cut(s) 61, 186
BmsI GCATC 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 108, 430
Bse3DI GCAATG 2 cut(s) 52, 216
BseBI CCWGG 2 cut(s) 61, 186
BseDI CCNNGG 1 cut(s) 482
BseMI GCAATG 2 cut(s) 52, 216
BseMII CTCAG 1 cut(s) 238
BseNI ACTGG 2 cut(s) 108, 430
BseXI GCAGC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 325
BslFI GGGAC 1 cut(s) 424
BsmAI GTCTC 2 cut(s) 139, 194
BsmFI GGGAC 1 cut(s) 424
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 268
Bsp143I GATC 1 cut(s) 463
BspACI CCGC 2 cut(s) 95, 389
BspCNI CTCAG 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 58, 201
BspQI GCTCTTC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 56
BspTI CTTAAG 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 52, 216
BsrGI TGTACA 1 cut(s) 268
BsrI ACTGG 2 cut(s) 108, 430
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 61, 186
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 212, 224, 392
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 2 cut(s) 139, 194
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 2 cut(s) 61, 186
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 2 cut(s) 59, 184
BstV1I GCAGC 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 423
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 105, 199
CsiI ACCWGGT 1 cut(s) 184
Csp6I GTAC 3 cut(s) 179, 269, 277
CviAII CATG 4 cut(s) 102, 172, 196, 272
CviJI RGCY 6 cut(s) 31, 82, 123, 158, 230, 266
CviKI_1 RGCY 6 cut(s) 31, 82, 123, 158, 230, 266
CviQI GTAC 3 cut(s) 179, 269, 277
DdeI CTNAG 3 cut(s) 212, 224, 392
DinI GGCGCC 1 cut(s) 58
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 105, 199
Eco57I CTGAAG 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 59, 184
EcoT14I CCWWGG 1 cut(s) 482
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 4 cut(s) 105, 175, 199, 275
FaqI GGGAC 1 cut(s) 424
FatI CATG 4 cut(s) 101, 171, 195, 271
Fnu4HI GCNGC 2 cut(s) 29, 389
Fsp4HI GCNGC 2 cut(s) 29, 389
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 58
GluI GCNGC 2 cut(s) 29, 389
HaeII RGCGCY 1 cut(s) 60
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 4 cut(s) 105, 175, 199, 275
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 1 cut(s) 132
HphI GGTGA 3 cut(s) 440, 449, 470
Hpy166II GTNNAC 2 cut(s) 236, 301
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 2 cut(s) 236, 301
HpyAV CCTTC 2 cut(s) 337, 355
HpyCH4V TGCA 3 cut(s) 45, 195, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 3 cut(s) 212, 224, 392
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 4 cut(s) 105, 175, 199, 275
HspAI GCGC 1 cut(s) 57
KasI GGCGCC 1 cut(s) 56
Kzo9I GATC 1 cut(s) 463
LguI GCTCTTC 1 cut(s) 330
LpnPI CCDG 9 cut(s) 46, 73, 105, 121, 171, 198, 211, 393, 411
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 1 cut(s) 82
MabI ACCWGGT 1 cut(s) 184
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 472
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 4 cut(s) 76, 200, 317, 458
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 4 cut(s) 39, 372, 432, 450
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 3 cut(s) 51, 84, 246
MspCI CTTAAG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 61, 186
MvaI CCWGG 2 cut(s) 61, 186
MwoI GCNNNNNNNGC 1 cut(s) 227
NarI GGCGCC 1 cut(s) 57
NdeII GATC 1 cut(s) 463
NlaIII CATG 4 cut(s) 105, 175, 199, 275
NlaIV GGNNCC 2 cut(s) 58, 201
NspI RCATGY 1 cut(s) 175
PciI ACATGT 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 68
PkrI GCNGC 2 cut(s) 30, 390
PleI GAGTC 1 cut(s) 140
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 140
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 59, 184
PspGI CCWGG 2 cut(s) 59, 184
PspN4I GGNNCC 2 cut(s) 58, 201
PspPI GGNCC 2 cut(s) 105, 199
RsaI GTAC 3 cut(s) 180, 270, 278
RsaNI GTAC 3 cut(s) 179, 269, 277
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 51, 84, 246
SatI GCNGC 2 cut(s) 29, 389
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 2 cut(s) 105, 199
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 2 cut(s) 61, 186
SduI GDGCHC 1 cut(s) 325
SetI ASST 6 cut(s) 33, 160, 190, 268, 348, 488
SexAI ACCWGGT 1 cut(s) 184
SfaNI GCATC 1 cut(s) 82
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 2 cut(s) 105, 199
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 4 cut(s) 39, 372, 432, 450
SsiI CCGC 2 cut(s) 95, 389
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 59, 184
StyI CCWWGG 1 cut(s) 482
TaqI TCGA 1 cut(s) 378
TasI AATT 4 cut(s) 39, 372, 432, 450
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 391
Tru1I TTAA 3 cut(s) 51, 84, 246
Tru9I TTAA 3 cut(s) 51, 84, 246
TscAI CASTG 1 cut(s) 430
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 90, 356
TspRI CASTG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 105, 199
XapI RAATTY 1 cut(s) 39
XceI RCATGY 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.