Rh2CG148000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012561
Physical Location & Seq
Reverse (-)
12965847 .. 12966612
766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG148000.1

Sequence Viewer

Length: 705 bp
ATGTGGGTGCAGCCAGGTCTCTGCATGGACCCTTTTCTTCTCAGCATTGCGCTCAAGGCTTGTGGACTTGGAAGGAACTTGTCTTATGGGGAAGTTGTACATGGGTACTCGGTGAAATCGGGTTTTGTGAACTCAGTCTTTGTGGGTAGTGCTCTTCTTGACATGTATATGAAGGTTGGTGAGATTGAGAAGGGTTGTAGAGTTTTCGACGCAATGCCGCTGAGAAATGTGGTGTCTTGGACTACCATTATCACTGGTCTCGTTCGCGCAGGTTATAATGTGGAGGGGTTGCGATATTTTGCTGAAATGTGGAGGTGTAGGGTGCAATGTGATGCGTATGCGTTTGCTATCTCGTTGAAGGCGTGTGCTGATTTGGGTGCTTTGAAGTGTGGGAGAGAAATTCACACGCAAACGATGAAGAAAGGGTTTGATGAGAACTCTTTCGTAGCCAACTCTCTTGCTACCATGTACAATAAATGTGGAAAATTGGACTATGGGGTGCAGTTGTTCACGAACATGAGGACGCAGGATGTTGTTTCGTGGACAGCAATCATTACATCGTATTTTTGGACGGGTCAAGAGGACCATGCAATCAAAGCATTTATGCGAATGCAGGAATCTGAGTTGAATGGGGTGAACAATTGCATGCCAACGTGTTACGCTTGGGTCTTGCGGATTCTTTGTCAGTGGGAAATTCAGTTATAG

Protein Analysis

234

Amino Acids

26.39

Weight (kDa)

7.97

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 123 1.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 260
AccII CGCG 1 cut(s) 267
AciI CCGC 2 cut(s) 218, 673
AcsI RAATTY 2 cut(s) 399, 693
AfaI GTAC 3 cut(s) 99, 107, 470
AflIII ACRYGT 2 cut(s) 162, 653
AgsI TTSAA 3 cut(s) 358, 385, 628
AjnI CCWGG 1 cut(s) 13
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 2 cut(s) 23, 263
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 399, 693
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 2 cut(s) 52, 269
AspS9I GGNCC 2 cut(s) 28, 583
AsuHPI GGTGA 3 cut(s) 124, 191, 646
AvaII GGWCC 2 cut(s) 28, 583
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 23, 263
BfuAI ACCTGC 1 cut(s) 260
BisI GCNGC 2 cut(s) 11, 218
BlsI GCNGC 2 cut(s) 12, 219
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 28, 583
BmgT120I GGNCC 2 cut(s) 28, 583
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 38
BsaI GGTCTC 2 cut(s) 23, 263
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 3 cut(s) 45, 219, 332
BseBI CCWGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 535
BseMI GCAATG 3 cut(s) 45, 219, 332
BseMII CTCAG 4 cut(s) 55, 147, 212, 612
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 29, 521
Bsh1236I CGCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 23, 263
BsmI GAATGC 1 cut(s) 615
Bso31I GGTCTC 2 cut(s) 23, 263
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 2 cut(s) 97, 468
BspACI CCGC 2 cut(s) 218, 673
BspCNI CTCAG 4 cut(s) 54, 146, 213, 613
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 159
BspTNI GGTCTC 2 cut(s) 23, 263
BsrDI GCAATG 3 cut(s) 45, 219, 332
BsrGI TGTACA 2 cut(s) 97, 468
BsrI ACTGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 15
Bst6I CTCTTC 1 cut(s) 159
BstAUI TGTACA 2 cut(s) 97, 468
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 4 cut(s) 41, 133, 221, 621
BstF5I GGATG 1 cut(s) 535
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 2 cut(s) 52, 269
BstMAI GTCTC 2 cut(s) 23, 263
BstMWI GCNNNNNNNGC 2 cut(s) 56, 596
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 2 cut(s) 166, 649
BstSCI CCNGG 1 cut(s) 13
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 535
BtsIMutI CAGTG 2 cut(s) 252, 692
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 647
CfoI GCGC 2 cut(s) 52, 269
Cfr13I GGNCC 2 cut(s) 28, 583
CseI GACGC 2 cut(s) 218, 532
Csp6I GTAC 3 cut(s) 98, 106, 469
CspCI CAANNNNNGTGG 4 cut(s) 43, 78, 460, 495
CviAII CATG 7 cut(s) 25, 101, 163, 466, 517, 587, 646
CviJI RGCY 3 cut(s) 13, 59, 449
CviKI_1 RGCY 3 cut(s) 13, 59, 449
CviQI GTAC 3 cut(s) 98, 106, 469
DdeI CTNAG 4 cut(s) 41, 133, 221, 621
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco31I GGTCTC 2 cut(s) 23, 263
Eco47I GGWCC 2 cut(s) 28, 583
EcoRII CCWGG 1 cut(s) 13
FaeI CATG 7 cut(s) 28, 104, 166, 469, 520, 590, 649
FatI CATG 7 cut(s) 24, 100, 162, 465, 516, 586, 645
Fnu4HI GCNGC 2 cut(s) 11, 218
FokI GGATG 1 cut(s) 542
Fsp4HI GCNGC 2 cut(s) 11, 218
GlaI GCGC 2 cut(s) 51, 268
GluI GCNGC 2 cut(s) 11, 218
HgaI GACGC 2 cut(s) 218, 532
HhaI GCGC 2 cut(s) 52, 269
Hin1II CATG 7 cut(s) 28, 104, 166, 469, 520, 590, 649
Hin6I GCGC 2 cut(s) 50, 267
HinP1I GCGC 2 cut(s) 50, 267
HinfI GANTC 2 cut(s) 617, 676
HphI GGTGA 3 cut(s) 124, 191, 646
Hpy166II GTNNAC 5 cut(s) 65, 130, 510, 543, 637
Hpy188I TCNGA 1 cut(s) 622
Hpy188III TCNNGA 3 cut(s) 158, 511, 578
Hpy8I GTNNAC 5 cut(s) 65, 130, 510, 543, 637
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 4 cut(s) 66, 166, 184, 352
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 7 cut(s) 10, 24, 325, 502, 590, 613, 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 596
HpyF3I CTNAG 4 cut(s) 41, 133, 221, 621
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 7 cut(s) 28, 104, 166, 469, 520, 590, 649
HspAI GCGC 2 cut(s) 50, 267
LguI GCTCTTC 1 cut(s) 159
LpnPI CCDG 5 cut(s) 27, 240, 255, 512, 599
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 322
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 1 cut(s) 656
MboII GAAGA 3 cut(s) 29, 146, 430
MfeI CAATTG 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 4 cut(s) 399, 485, 640, 693
MnlI CCTC 4 cut(s) 277, 306, 513, 574
MroXI GAANNNNTTC 1 cut(s) 440
MslI CAYNNNNRTG 2 cut(s) 167, 515
MspA1I CMGCKG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 640
Mva1269I GAATGC 1 cut(s) 615
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 56, 596
NlaIII CATG 7 cut(s) 28, 104, 166, 469, 520, 590, 649
NlaIV GGNNCC 1 cut(s) 30
NspI RCATGY 2 cut(s) 166, 649
PaeI GCATGC 1 cut(s) 649
PciI ACATGT 1 cut(s) 162
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 359
PctI GAATGC 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 2 cut(s) 617, 676
PkrI GCNGC 2 cut(s) 12, 219
PscI ACATGT 1 cut(s) 162
PsiI TTATAA 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 2 cut(s) 28, 583
RsaI GTAC 3 cut(s) 99, 107, 470
RsaNI GTAC 3 cut(s) 98, 106, 469
RseI CAYNNNNRTG 2 cut(s) 167, 515
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 2 cut(s) 11, 218
Sau96I GGNCC 2 cut(s) 28, 583
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 154
SetI ASST 5 cut(s) 19, 177, 274, 317, 656
SfaNI GCATC 1 cut(s) 322
SinI GGWCC 2 cut(s) 28, 583
SmiMI CAYNNNNRTG 2 cut(s) 167, 515
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SphI GCATGC 1 cut(s) 649
Sse9I AATT 4 cut(s) 399, 485, 640, 693
SsiI CCGC 2 cut(s) 218, 673
StyD4I CCNGG 1 cut(s) 13
TaiI ACGT 1 cut(s) 656
TaqI TCGA 1 cut(s) 207
TasI AATT 4 cut(s) 399, 485, 640, 693
TatI WGTACW 2 cut(s) 97, 468
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 2 cut(s) 617, 676
TscAI CASTG 2 cut(s) 259, 692
TseI GCWGC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 185, 431
TspRI CASTG 2 cut(s) 259, 692
VpaK11BI GGWCC 2 cut(s) 28, 583
XapI RAATTY 2 cut(s) 399, 693
XceI RCATGY 2 cut(s) 166, 649
XmnI GAANNNNTTC 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.