Rh7BG234300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012561
Physical Location & Seq
Reverse (-)
20483352 .. 20484897
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG234300.1

Sequence Viewer

Length: 795 bp
ATGCTCGAACTCAACTCTCAACTGAAGCAGCTAGTCAAAATTTGCAATGTTAAAGGCGCTTGGAAGATGTTCGATAAAAGGCCTAAGAGAGATGCGGTTTCATGGACCAGTATGATTTCAGGCTATGTTAGAGTCTCTGATTCCTCTAAAGCATTAGTTCTGTTCTCTAACATGTGGGTACAGCCAGGTCTCTGCATGGACCATTTTCTTCTTAGCATTGCACTCAGGGCTTGTGGCTTTGGTATTAACTTGTCTTATGGGGAAGCTGTACATGGGTACTTGGCGAAATCGGGTATTGTGAACTCTGTCTTTGTGGGAAGTGCTCTTCTTGACTTGTATATGAAGGTTGTTGGGATTGAGAAGGGTTGTATAATTTTCGACAAAATGCCGTTAAGAAATGTGGTGTCTGGGACTACCATTATCACTGGTGAAATTGGCGAGCAAGTTCAAATTGTTGGTGATGATCTCCTTGTTACAAACCTAAAGCGAAGGGAGAAAGCAATCAAAGAGAAGAGCTGTAATGCCCTTCTTTTGAAGGTATTTGTGCACTGTTCTGACCATGCTCTCTTCAATGGCAGCACTACTTCAACCGCAGGCCAAGACAATTTTACTGTTGAGGGCAATGTAACTATCGCTAAAAACCCGTGTGTGCACCTGGGAGATGAGCGTTTTCTTATGGATGGAGTGAGTGAGGTTTCTGATCATTATGCTTGGCTGGACTTTCTAACAAGCGATGTTCCCGGGACTAAGGTGAGATCTATAAGCATCGTTCTATGGTTGTGGAAGCTTCTATAG

Protein Analysis

264

Amino Acids

29.15

Weight (kDa)

8.42

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_C PF00113 132 - 180 8e-12 Enolase, C-terminal TIM barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 95, 591
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 3 cut(s) 180, 270, 278
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 4 cut(s) 449, 535, 571, 588
AjnI CCWGG 2 cut(s) 184, 654
AluBI AGCT 4 cut(s) 31, 266, 516, 787
AluI AGCT 4 cut(s) 31, 266, 516, 787
Alw21I GWGCWC 3 cut(s) 325, 549, 654
Alw26I GTCTC 2 cut(s) 139, 194
Alw44I GTGCAC 2 cut(s) 545, 650
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 2 cut(s) 80, 595
ApaLI GTGCAC 2 cut(s) 545, 650
ApeKI GCWGC 2 cut(s) 28, 576
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 2 cut(s) 105, 199
AsuC2I CCSGG 2 cut(s) 741, 742
AsuHPI GGTGA 3 cut(s) 440, 470, 763
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 2 cut(s) 105, 199
BaeGI GKGCMC 2 cut(s) 549, 654
Bbv12I GWGCWC 3 cut(s) 325, 549, 654
BbvI GCAGC 2 cut(s) 40, 588
BccI CCATC 1 cut(s) 674
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 2 cut(s) 186, 656
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 2 cut(s) 741, 742
BcoDI GTCTC 2 cut(s) 139, 194
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 791
BfoI RGCGCY 1 cut(s) 60
BglII AGATCT 1 cut(s) 755
BisI GCNGC 2 cut(s) 29, 577
BlsI GCNGC 2 cut(s) 30, 578
Bme1390I CCNGG 4 cut(s) 186, 656, 741, 742
Bme18I GGWCC 2 cut(s) 105, 199
BmeT110I CYCGRG 1 cut(s) 740
BmgT120I GGNCC 2 cut(s) 105, 199
BmrFI CCNGG 4 cut(s) 186, 656, 741, 742
BmsI GCATC 2 cut(s) 82, 774
BpuMI CCSGG 2 cut(s) 741, 742
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 655, 740
Bse1I ACTGG 2 cut(s) 108, 430
Bse3DI GCAATG 3 cut(s) 52, 216, 628
BseBI CCWGG 2 cut(s) 186, 656
BseDI CCNNGG 2 cut(s) 655, 740
BseGI GGATG 1 cut(s) 685
BseMI GCAATG 3 cut(s) 52, 216, 628
BseMII CTCAG 1 cut(s) 238
BseNI ACTGG 2 cut(s) 108, 430
BseSI GKGCMC 2 cut(s) 549, 654
BseXI GCAGC 2 cut(s) 40, 588
BshFI GGCC 2 cut(s) 82, 597
BsiHKAI GWGCWC 3 cut(s) 325, 549, 654
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 1 cut(s) 741
BslFI GGGAC 2 cut(s) 424, 757
BsmAI GTCTC 2 cut(s) 139, 194
BsmFI GGGAC 2 cut(s) 424, 757
BsnI GGCC 2 cut(s) 82, 597
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 325, 549, 654
Bsp1407I TGTACA 1 cut(s) 268
Bsp143I GATC 3 cut(s) 463, 700, 755
BspACI CCGC 2 cut(s) 95, 591
BspANI GGCC 2 cut(s) 82, 597
BspCNI CTCAG 1 cut(s) 237
BspQI GCTCTTC 2 cut(s) 330, 506
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 3 cut(s) 52, 216, 628
BsrGI TGTACA 1 cut(s) 268
BsrI ACTGG 2 cut(s) 108, 430
BssECI CCNNGG 2 cut(s) 655, 740
BssMI GATC 3 cut(s) 463, 700, 755
Bst2UI CCWGG 2 cut(s) 186, 656
Bst4CI ACNGT 2 cut(s) 551, 613
Bst6I CTCTTC 3 cut(s) 330, 506, 572
BstAUI TGTACA 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 440, 595
BstDEI CTNAG 4 cut(s) 84, 212, 224, 747
BstF5I GGATG 1 cut(s) 685
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 3 cut(s) 466, 703, 758
BstMAI GTCTC 2 cut(s) 139, 194
BstMBI GATC 3 cut(s) 463, 700, 755
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 2 cut(s) 186, 656
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 4 cut(s) 184, 654, 739, 740
BstSFI CTRYAG 1 cut(s) 791
BstSLI GKGCMC 2 cut(s) 549, 654
BstV1I GCAGC 2 cut(s) 40, 588
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
BsuRI GGCC 2 cut(s) 82, 597
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 1 cut(s) 685
BtsIMutI CAGTG 2 cut(s) 423, 547
Cac8I GCNNGC 2 cut(s) 440, 595
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 105, 199
Cfr9I CCCGGG 1 cut(s) 740
Csp6I GTAC 3 cut(s) 179, 269, 277
CviAII CATG 5 cut(s) 102, 172, 196, 272, 560
CviQI GTAC 3 cut(s) 179, 269, 277
DdeI CTNAG 4 cut(s) 84, 212, 224, 747
DpnI GATC 3 cut(s) 465, 702, 757
DpnII GATC 3 cut(s) 463, 700, 755
Eam1104I CTCTTC 3 cut(s) 330, 506, 572
EarI CTCTTC 3 cut(s) 330, 506, 572
Eco147I AGGCCT 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 105, 199
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 184, 654
FaeI CATG 5 cut(s) 105, 175, 199, 275, 563
FaqI GGGAC 2 cut(s) 424, 757
FatI CATG 5 cut(s) 101, 171, 195, 271, 559
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 2 cut(s) 29, 577
FokI GGATG 1 cut(s) 692
Fsp4HI GCNGC 2 cut(s) 29, 577
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 58
GluI GCNGC 2 cut(s) 29, 577
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 2 cut(s) 82, 597
HapII CCGG 1 cut(s) 741
HhaI GCGC 1 cut(s) 59
Hin1II CATG 5 cut(s) 105, 175, 199, 275, 563
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 2 cut(s) 132, 140
HpaII CCGG 1 cut(s) 741
HphI GGTGA 3 cut(s) 440, 470, 763
Hpy166II GTNNAC 3 cut(s) 301, 547, 652
Hpy188I TCNGA 3 cut(s) 139, 556, 700
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 3 cut(s) 301, 547, 652
HpyAV CCTTC 5 cut(s) 337, 355, 483, 529, 536
HpyCH4III ACNGT 2 cut(s) 551, 613
HpyCH4V TGCA 5 cut(s) 45, 195, 221, 547, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 4 cut(s) 84, 212, 224, 747
Hsp92II CATG 5 cut(s) 105, 175, 199, 275, 563
HspAI GCGC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 3 cut(s) 463, 700, 755
LguI GCTCTTC 2 cut(s) 330, 506
Lsp1109I GCAGC 2 cut(s) 40, 588
LweI GCATC 2 cut(s) 82, 774
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 472, 625
MalI GATC 3 cut(s) 465, 702, 757
MboI GATC 3 cut(s) 463, 700, 755
MboII GAAGA 5 cut(s) 76, 200, 317, 523, 559
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 3 cut(s) 325, 549, 654
MluCI AATT 5 cut(s) 39, 372, 432, 450, 604
MlyI GAGTC 1 cut(s) 141
MnlI CCTC 3 cut(s) 154, 610, 685
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 3 cut(s) 51, 246, 392
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 4 cut(s) 186, 656, 741, 742
MvaI CCWGG 2 cut(s) 186, 656
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 2 cut(s) 741, 742
NdeII GATC 3 cut(s) 463, 700, 755
NlaIII CATG 5 cut(s) 105, 175, 199, 275, 563
NspI RCATGY 1 cut(s) 175
PceI AGGCCT 1 cut(s) 82
PciI ACATGT 1 cut(s) 171
PciSI GCTCTTC 2 cut(s) 330, 506
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 30, 578
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 184, 654
PspGI CCWGG 2 cut(s) 184, 654
PspPI GGNCC 2 cut(s) 105, 199
PsuI RGATCY 1 cut(s) 755
RsaI GTAC 3 cut(s) 180, 270, 278
RsaNI GTAC 3 cut(s) 179, 269, 277
SapI GCTCTTC 2 cut(s) 330, 506
SaqAI TTAA 3 cut(s) 51, 246, 392
SatI GCNGC 2 cut(s) 29, 577
Sau3AI GATC 3 cut(s) 463, 700, 755
Sau96I GGNCC 2 cut(s) 105, 199
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 4 cut(s) 186, 656, 741, 742
SduI GDGCHC 3 cut(s) 325, 549, 654
SfaNI GCATC 2 cut(s) 82, 774
SfcI CTRYAG 1 cut(s) 791
SinI GGWCC 2 cut(s) 105, 199
SmaI CCCGGG 1 cut(s) 742
Sse9I AATT 5 cut(s) 39, 372, 432, 450, 604
SseBI AGGCCT 1 cut(s) 82
SsiI CCGC 2 cut(s) 95, 591
SspMI CTAG 1 cut(s) 32
StuI AGGCCT 1 cut(s) 82
StyD4I CCNGG 4 cut(s) 184, 654, 739, 740
TaaI ACNGT 2 cut(s) 551, 613
TaqI TCGA 3 cut(s) 6, 72, 378
TasI AATT 5 cut(s) 39, 372, 432, 450, 604
TatI WGTACW 1 cut(s) 268
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 3 cut(s) 51, 246, 392
Tru9I TTAA 3 cut(s) 51, 246, 392
TscAI CASTG 2 cut(s) 430, 554
TseI GCWGC 2 cut(s) 28, 576
TspDTI ATGAA 2 cut(s) 90, 356
TspMI CCCGGG 1 cut(s) 740
TspRI CASTG 2 cut(s) 430, 554
VneI GTGCAC 2 cut(s) 545, 650
VpaK11BI GGWCC 2 cut(s) 105, 199
XapI RAATTY 1 cut(s) 39
XceI RCATGY 1 cut(s) 175
XmaI CCCGGG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.