RchiOBHm_Chr7g0221331

diphthine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004448
Physical Location & Seq
Reverse (-)
42347007 .. 42348369
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19804

Sequence Viewer

Length: 744 bp
ATGTTGTACATAGTAGGTCTTGGATTGGGGAACGAGAAAGACATCACTTTGAGAGGTTTAGAAGCAGTTCAACAATGTGACAAGATCATCGTTGAAGCCTACACTTCTCTGCTCTCTTTTGGTCTCTCTTCCCACGGTCTTATCACCCTTGAAAAGTTGTATGGGAAACCGGTTATACTTGCAGATAGAGAAACCGTGGAGGAAAAGGCCGATGATATTTTAAGGGCAGCCGCTGATTTTGACGTCGCTTTTCTTGTTGTTGGGGATCCTTTTGGGTATTTCGGGATTAATGTCAAAGTGGTGCATAATGCATCGGTTATGAATGCGGTTAGAGTCTGTGGCTTGCAGCTCTACCATTATGGAGAGACAGTTTCGATACCATTCTTCACGGTTACATGGAGACCTGATAGCTTTTATGAGAAGATTCAGAAAAATCGTGGGCTTGGACTACATACTCTACTTGTTAGAGGAAAGAAGCAGTATGAACCACCTAGATATATGTCAGTAAACACTGCAATTGAACAGCTTTTGGAGGTTGAGCAAAACCGAGGAGAATTAGCATACAGCGAAGACACCATGTGTGTTGGGTTTGCTCGGCTTGGAAGTGAGGATCAGAAGATGGTTGCTGGTATAACGAGGCAACTGGAGTCGGTTGATTTTGGAGCACCTCTTCATTGCCTTGTAATAGTAGGCAAGACCCACCCTGTGGAAGAAGAAATGCTGGACTTCTACAGAACTGTGTGA

Protein Analysis

247

Amino Acids

27.61

Weight (kDa)

5.2

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 1 - 211 5.6e-15 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 706
AciI CCGC 2 cut(s) 231, 326
AclWI GGATC 3 cut(s) 260, 273, 618
AcyI GRCGYC 1 cut(s) 243
AdeI CACNNNGTG 2 cut(s) 579, 706
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 706
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 4 cut(s) 71, 95, 152, 521
AluBI AGCT 3 cut(s) 349, 411, 526
AluI AGCT 3 cut(s) 349, 411, 526
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 3 cut(s) 128, 359, 394
AlwI GGATC 3 cut(s) 260, 273, 618
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 1 cut(s) 207
ApeKI GCWGC 2 cut(s) 227, 346
AseI ATTAAT 1 cut(s) 288
AsiGI ACCGGT 1 cut(s) 169
Asp700I GAANNNNTTC 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 136
BamHI GGATCC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 576
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 239, 358
BccI CCATC 1 cut(s) 613
BcoDI GTCTC 3 cut(s) 128, 359, 394
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 730
BisI GCNGC 3 cut(s) 228, 231, 347
BlsI GCNGC 3 cut(s) 229, 232, 348
BmiI GGNNCC 1 cut(s) 267
BmsI GCATC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 576
BpmI CTGGAG 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 2 cut(s) 128, 394
BsaJI CCNNGG 3 cut(s) 133, 195, 547
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 706
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 648
Bse3DI GCAATG 1 cut(s) 673
BseDI CCNNGG 3 cut(s) 133, 195, 547
BseLI CCNNNNNNNGG 1 cut(s) 706
BseMI GCAATG 1 cut(s) 673
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 1 cut(s) 564
BseXI GCAGC 2 cut(s) 239, 358
BshFI GGCC 1 cut(s) 209
BshTI ACCGGT 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 706
BsmAI GTCTC 3 cut(s) 128, 359, 394
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 209
Bso31I GGTCTC 2 cut(s) 128, 394
Bsp1286I GDGCHC 1 cut(s) 667
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 3 cut(s) 84, 265, 610
BspACI CCGC 2 cut(s) 231, 326
BspANI GGCC 1 cut(s) 209
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 3 cut(s) 260, 273, 618
BspTNI GGTCTC 2 cut(s) 128, 394
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 1 cut(s) 648
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 133, 195, 547
BssMI GATC 3 cut(s) 84, 265, 610
BssNI GRCGYC 1 cut(s) 243
Bst4CI ACNGT 5 cut(s) 137, 196, 370, 391, 739
Bst6I CTCTTC 2 cut(s) 133, 675
BstACI GRCGYC 1 cut(s) 243
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 1 cut(s) 344
BstDSI CCRYGG 2 cut(s) 133, 195
BstKTI GATC 3 cut(s) 87, 268, 613
BstMAI GTCTC 3 cut(s) 128, 359, 394
BstMBI GATC 3 cut(s) 84, 265, 610
BstSFI CTRYAG 1 cut(s) 730
BstV1I GCAGC 2 cut(s) 239, 358
BstV2I GAAGAC 1 cut(s) 576
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 209
BtgI CCRYGG 2 cut(s) 133, 195
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 1 cut(s) 344
CaiI CAGNNNCTG 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 169
Csp6I GTAC 1 cut(s) 7
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 2 cut(s) 396, 577
CviJI RGCY 9 cut(s) 98, 209, 230, 342, 349, 411, 442, 526, 598
CviKI_1 RGCY 9 cut(s) 98, 209, 230, 342, 349, 411, 442, 526, 598
CviQI GTAC 1 cut(s) 7
DpnI GATC 3 cut(s) 86, 267, 612
DpnII GATC 3 cut(s) 84, 265, 610
DraIII CACNNNGTG 2 cut(s) 579, 706
Eam1104I CTCTTC 2 cut(s) 133, 675
EarI CTCTTC 2 cut(s) 133, 675
Eco31I GGTCTC 2 cut(s) 128, 394
EcoT22I ATGCAT 1 cut(s) 313
FaeI CATG 2 cut(s) 399, 580
FatI CATG 2 cut(s) 395, 576
Fnu4HI GCNGC 3 cut(s) 228, 231, 347
Fsp4HI GCNGC 3 cut(s) 228, 231, 347
FspBI CTAG 1 cut(s) 492
GluI GCNGC 3 cut(s) 228, 231, 347
GsuI CTGGAG 1 cut(s) 665
HaeIII GGCC 1 cut(s) 209
HapII CCGG 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 2 cut(s) 399, 580
HinfI GANTC 3 cut(s) 333, 424, 647
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 2 cut(s) 429, 615
Hpy188III TCNNGA 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 248
HpyCH4III ACNGT 5 cut(s) 137, 196, 370, 391, 739
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 182, 304, 311, 346, 515
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 2 cut(s) 399, 580
Kzo9I GATC 3 cut(s) 84, 265, 610
LmnI GCTCC 1 cut(s) 662
LpnPI CCDG 6 cut(s) 183, 417, 612, 629, 707, 717
Lsp1109I GCAGC 2 cut(s) 239, 358
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 77, 391
MalI GATC 3 cut(s) 86, 267, 612
MboI GATC 3 cut(s) 84, 265, 610
MboII GAAGA 8 cut(s) 120, 376, 433, 581, 628, 662, 722, 725
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 2 cut(s) 516, 554
MlyI GAGTC 2 cut(s) 342, 656
MnlI CCTC 8 cut(s) 47, 193, 461, 526, 542, 601, 630, 678
Mph1103I ATGCAT 1 cut(s) 313
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 221, 288
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 1 cut(s) 170
MunI CAATTG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 328
NdeII GATC 3 cut(s) 84, 265, 610
NlaIII CATG 2 cut(s) 399, 580
NlaIV GGNNCC 1 cut(s) 267
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 313
PctI GAATGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 1 cut(s) 424
PflMI CCANNNNNTGG 1 cut(s) 706
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 3 cut(s) 229, 232, 348
PleI GAGTC 2 cut(s) 341, 655
PpsI GAGTC 2 cut(s) 341, 655
PshBI ATTAAT 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 2 cut(s) 221, 288
SatI GCNGC 3 cut(s) 228, 231, 347
Sau3AI GATC 3 cut(s) 84, 265, 610
SchI GAGTC 2 cut(s) 342, 656
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 1 cut(s) 320
SfcI CTRYAG 1 cut(s) 730
Sse9I AATT 2 cut(s) 516, 554
SsiI CCGC 2 cut(s) 231, 326
SspMI CTAG 1 cut(s) 492
TaaI ACNGT 5 cut(s) 137, 196, 370, 391, 739
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 374
TasI AATT 2 cut(s) 516, 554
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 2 cut(s) 221, 288
Tru9I TTAA 2 cut(s) 221, 288
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 2 cut(s) 227, 346
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 335, 498, 662
TspRI CASTG 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 706
VspI ATTAAT 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 1 cut(s) 492
ZraI GACGTC 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.