Rroxscaffold_3G00238400

diphthine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004448
Physical Location & Seq
Forward (+)
26529256 .. 26543507
14252 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00238400.1

Sequence Viewer

Length: 789 bp
ATGGCGGACTCGCCGGATACCGAGACTCGTACAAGCACATCCGGGGGCTCGCGTCGCCCGCAAGCGAGTGGGGGCGATCTTGACTCGATCGGCGAAGGGGATGTCGAGGTAGGCGAAGTGGCGCTCGGTGGTGGTGAGCCGATTGACGACGTTCTCGTTGCCGGCGCAGCCGTGGAAGATGAGGAGGTTCTCGAAGGCAATGAGGTTGTGGAGATGAGACTAAGAAAAAACAATTCTTTGCAGGAAAAGTTGTATGGGAAACCAGTTATACTTGCAGATAGGGAAACCGTGGAGGAAAAGGCCGATGATATTTTAAGTGCAGCCGCTGATTCTGACGTCGCTTTTCTTGTTGTTGGGGATCCTTTTGGAGCTACAACTCACACTGATCTTGTAGTTCGAGCTAAGAAGTTAGGGATTAATGTCAAAGTGGTGCATAATGCATCGGTTATGAATGCGGTTGGAGTCTGTGGCTTGCAGCTCTACCATTATGGAGAGACAGTTTCGATACCATTCTTCACGGATACATGGAGACCTGATAGCTTTTATGAGAAGATTCAGAAAAATCGTGGGCTTGGACTACATACTCTCTGCTTGTTAGAAATGCATACGGCGAAGACACCATGTGTGTTGGGTTTGCTCGGCTTGGAAGTGAGGATCGAGAAGATAGTTGTTGGTACAATGAGGCAACTGGAGTCGGTTGATTTTGGAGCACCTCTTCATTGCCTTGTAATAGTAGGCAAGACCCACCCGGTGGAAGAAGAAATGCTGGACTTCTACGGAACCGTGTGA

Protein Analysis

262

Amino Acids

28.19

Weight (kDa)

4.64

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 94 - 200 4.3e-11 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 339
AccB7I CCANNNNNTGG 1 cut(s) 751
AccII CGCG 1 cut(s) 52
AciI CCGC 4 cut(s) 5, 59, 324, 455
AclWI GGATC 3 cut(s) 353, 366, 662
AcyI GRCGYC 1 cut(s) 336
AdeI CACNNNGTG 2 cut(s) 623, 751
AfaI GTAC 2 cut(s) 31, 676
AfiI CCNNNNNNNGG 1 cut(s) 751
AluBI AGCT 4 cut(s) 371, 401, 478, 540
AluI AGCT 4 cut(s) 371, 401, 478, 540
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 4 cut(s) 17, 211, 488, 523
AlwI GGATC 3 cut(s) 353, 366, 662
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 167, 320, 475
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AseI ATTAAT 1 cut(s) 417
AspLEI GCGC 2 cut(s) 124, 167
AsuC2I CCSGG 2 cut(s) 43, 749
AsuHPI GGTGA 1 cut(s) 146
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 3 cut(s) 179, 332, 487
BceAI ACGGC 2 cut(s) 155, 624
BciVI GTATCC 2 cut(s) 10, 514
BcnI CCSGG 2 cut(s) 43, 749
BcoDI GTCTC 4 cut(s) 17, 211, 488, 523
BfoI RGCGCY 1 cut(s) 125
BfuI GTATCC 2 cut(s) 10, 514
BisI GCNGC 4 cut(s) 168, 321, 324, 476
BlsI GCNGC 4 cut(s) 169, 322, 325, 477
Bme1390I CCNGG 2 cut(s) 43, 749
BmiI GGNNCC 2 cut(s) 360, 781
BmrFI CCNGG 2 cut(s) 43, 749
BmsI GCATC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 1 cut(s) 710
BpuMI CCSGG 2 cut(s) 43, 749
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 523
BsaJI CCNNGG 3 cut(s) 42, 171, 288
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 751
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 263, 693
Bse3DI GCAATG 2 cut(s) 205, 718
BseDI CCNNGG 3 cut(s) 42, 171, 288
BseGI GGATG 2 cut(s) 38, 106
BseLI CCNNNNNNNGG 1 cut(s) 751
BseMI GCAATG 2 cut(s) 205, 718
BseNI ACTGG 2 cut(s) 263, 693
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 3 cut(s) 179, 332, 487
BsgI GTGCAG 1 cut(s) 339
Bsh1236I CGCG 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 4 cut(s) 14, 42, 162, 749
BslI CCNNNNNNNGG 1 cut(s) 751
BsmAI GTCTC 4 cut(s) 17, 211, 488, 523
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 1 cut(s) 302
Bso31I GGTCTC 1 cut(s) 523
Bsp1286I GDGCHC 2 cut(s) 50, 712
Bsp143I GATC 5 cut(s) 76, 87, 358, 385, 654
BspACI CCGC 4 cut(s) 5, 59, 324, 455
BspANI GGCC 1 cut(s) 302
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 360, 781
BspPI GGATC 3 cut(s) 353, 366, 662
BspTNI GGTCTC 1 cut(s) 523
BsrDI GCAATG 2 cut(s) 205, 718
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 2 cut(s) 263, 693
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 3 cut(s) 42, 171, 288
BssMI GATC 5 cut(s) 76, 87, 358, 385, 654
BssNI GRCGYC 1 cut(s) 336
Bst4CI ACNGT 3 cut(s) 289, 499, 784
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 336
BstC8I GCNNGC 5 cut(s) 50, 59, 63, 163, 473
BstDEI CTNAG 2 cut(s) 221, 402
BstDSI CCRYGG 2 cut(s) 171, 288
BstF5I GGATG 2 cut(s) 38, 106
BstFNI CGCG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 167
BstKTI GATC 5 cut(s) 79, 90, 361, 388, 657
BstMAI GTCTC 4 cut(s) 17, 211, 488, 523
BstMBI GATC 5 cut(s) 76, 87, 358, 385, 654
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 3 cut(s) 54, 58, 167
BstSCI CCNGG 2 cut(s) 41, 747
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 179, 332, 487
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 2 cut(s) 10, 514
BsuRI GGCC 1 cut(s) 302
BtgI CCRYGG 2 cut(s) 171, 288
BtsCI GGATG 2 cut(s) 38, 106
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 5 cut(s) 50, 59, 63, 163, 473
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 2 cut(s) 124, 167
Cfr10I RCCGGY 1 cut(s) 161
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 30, 675
CviAII CATG 2 cut(s) 525, 621
CviQI GTAC 2 cut(s) 30, 675
DdeI CTNAG 2 cut(s) 221, 402
DpnI GATC 5 cut(s) 78, 89, 360, 387, 656
DpnII GATC 5 cut(s) 76, 87, 358, 385, 654
DraIII CACNNNGTG 2 cut(s) 623, 751
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 523
EcoT22I ATGCAT 2 cut(s) 442, 606
EcoT38I GRGCYC 1 cut(s) 50
FaeI CATG 2 cut(s) 528, 624
FatI CATG 2 cut(s) 524, 620
FauI CCCGC 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 168, 321, 324, 476
FokI GGATG 2 cut(s) 25, 113
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 4 cut(s) 168, 321, 324, 476
GlaI GCGC 2 cut(s) 123, 166
GluI GCNGC 4 cut(s) 168, 321, 324, 476
GsuI CTGGAG 1 cut(s) 710
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 1 cut(s) 302
HapII CCGG 4 cut(s) 14, 42, 162, 749
HgaI GACGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 124, 167
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 2 cut(s) 528, 624
Hin6I GCGC 2 cut(s) 122, 165
HinP1I GCGC 2 cut(s) 122, 165
HinfI GANTC 7 cut(s) 8, 25, 83, 329, 462, 553, 692
HpaII CCGG 4 cut(s) 14, 42, 162, 749
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 2 cut(s) 334, 558
Hpy188III TCNNGA 3 cut(s) 80, 191, 658
Hpy99I CGWCG 3 cut(s) 57, 152, 341
HpyAV CCTTC 2 cut(s) 89, 188
HpyCH4III ACNGT 3 cut(s) 289, 499, 784
HpyCH4IV ACGT 2 cut(s) 150, 336
HpyCH4V TGCA 7 cut(s) 241, 275, 320, 433, 440, 475, 604
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 58, 167
HpyF3I CTNAG 2 cut(s) 221, 402
HpySE526I ACGT 2 cut(s) 150, 336
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 2 cut(s) 528, 624
HspAI GCGC 2 cut(s) 122, 165
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 76, 87, 358, 385, 654
LmnI GCTCC 2 cut(s) 368, 707
LpnPI CCDG 9 cut(s) 27, 55, 175, 227, 276, 546, 674, 752, 762
Lsp1109I GCAGC 3 cut(s) 179, 332, 487
LweI GCATC 1 cut(s) 449
MaeII ACGT 2 cut(s) 150, 336
MalI GATC 5 cut(s) 78, 89, 360, 387, 656
MboI GATC 5 cut(s) 76, 87, 358, 385, 654
MboII GAAGA 8 cut(s) 188, 505, 562, 625, 673, 707, 767, 770
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 50, 712
MluCI AATT 1 cut(s) 232
MlyI GAGTC 5 cut(s) 2, 19, 77, 471, 701
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 8 cut(s) 100, 175, 178, 196, 286, 645, 675, 723
Mph1103I ATGCAT 2 cut(s) 442, 606
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 2 cut(s) 314, 417
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 4 cut(s) 14, 42, 162, 749
MspR9I CCNGG 2 cut(s) 43, 749
Mva1269I GAATGC 1 cut(s) 457
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 3 cut(s) 54, 58, 167
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 2 cut(s) 43, 749
NdeII GATC 5 cut(s) 76, 87, 358, 385, 654
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 2 cut(s) 528, 624
NlaIV GGNNCC 2 cut(s) 360, 781
NmeAIII GCCGAG 1 cut(s) 618
NsiI ATGCAT 2 cut(s) 442, 606
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 153
PctI GAATGC 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 329, 553
PflMI CCANNNNNTGG 1 cut(s) 751
PkrI GCNGC 4 cut(s) 169, 322, 325, 477
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 5 cut(s) 2, 19, 77, 470, 700
PpsI GAGTC 5 cut(s) 2, 19, 77, 470, 700
PshBI ATTAAT 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 360, 781
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 358
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 2 cut(s) 31, 676
RsaNI GTAC 2 cut(s) 30, 675
SaqAI TTAA 2 cut(s) 314, 417
SatI GCNGC 4 cut(s) 168, 321, 324, 476
Sau3AI GATC 5 cut(s) 76, 87, 358, 385, 654
SchI GAGTC 5 cut(s) 2, 19, 77, 471, 701
ScrFI CCNGG 2 cut(s) 43, 749
SduI GDGCHC 2 cut(s) 50, 712
SfaNI GCATC 1 cut(s) 449
Sse9I AATT 1 cut(s) 232
SsiI CCGC 4 cut(s) 5, 59, 324, 455
StyD4I CCNGG 2 cut(s) 41, 747
TaaI ACNGT 3 cut(s) 289, 499, 784
TaiI ACGT 2 cut(s) 153, 339
TaqI TCGA 6 cut(s) 86, 105, 192, 397, 503, 657
TasI AATT 1 cut(s) 232
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 329, 553
Tru1I TTAA 2 cut(s) 314, 417
Tru9I TTAA 2 cut(s) 314, 417
TscAI CASTG 1 cut(s) 388
TseI GCWGC 3 cut(s) 167, 320, 475
TspDTI ATGAA 2 cut(s) 464, 707
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 751
VspI ATTAAT 1 cut(s) 417
ZraI GACGTC 1 cut(s) 337
Zsp2I ATGCAT 2 cut(s) 442, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.