Rh7BG332400

diphthine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004448
Physical Location & Seq
Reverse (-)
34467919 .. 34470109
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG332400.1

Sequence Viewer

Length: 960 bp
ATGGCATTTACGTTATTTCTCCAAAAAACAGAGGGCAGTAGCCAAAACAAGGTTTCATATAAATTCCTGAAACGAACAACGCCGTCTTCTTCCCTCTTCTCTTTTATACGAAAACTCACTGACTCAAGATCAAGAAAGATGTTGTACATAGTAGGTCTTGGATTGGGGAACGAGAAAGACATCACTTTGAGAGGTTTAGAAGCAGTTCAACAATGTGACAAGATCTTCGTTGAAGCCTACACTTCTCTCCTCTCTTTTGGTCTCTCTTCCCACGGTCTTATCACCCTTGAAAAGTTGTATGGGAAACCGGTTATACTTGCAGATAGAGAAACCGTGGAGGAAAAGGCCGATGATATTTTAAGTGCAGCCGCTGATTCTGACGTCGCTTTTCTTGTTGTTGGGGATCCTTTTGGAGCTACAACTCACACTGATCTTGTAGTTCGAGCTAAGAAGTTAGGGATTAATGTCAAAGTGGTGCATAATGCATCGGTTATGAATGCGGTTGGAGTCTGTGGCTTGCAGCTCTACCATTATGGAGAGACAGTTTCGATACCATTCTTCACGGATACATGGAGACCTGATAGCTTTTATGAGAAGATTCAGAAAAATCGTGGGCTTGGACTACATACTCTCTGCTTGTTAGATATACGCGTGAAAGAACCTACCTTGGAATCTTTATGCAGAGGAAAGAAGCAGTATGAACCACCTAGATATATGTCAGTAAACACTGCAATTGAACAGCTTTTGGAGGTTGAGCAAAACCGAGGAGAATCAGCATACAGCGAAGACACCATGTGTGTTGGGTTTGCTCGGCTTGGAAGTGAGGATCAGAAGATAGTTGCTGGTACAATGAGGCAACTGGAGTCGGTTGATTTTGGAGCACCTCTTCATTGCCTTGTAATAGTAGGCAAGACCCACCCAGTGGAAGAAGAAATGCTGGACTTCTACAGAACTGTGTGA

Protein Analysis

319

Amino Acids

35.57

Weight (kDa)

6.2

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 47 - 287 1.8e-25 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 922
AccII CGCG 1 cut(s) 651
AciI CCGC 2 cut(s) 369, 500
AclWI GGATC 3 cut(s) 398, 411, 834
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 381
AdeI CACNNNGTG 2 cut(s) 795, 922
AfaI GTAC 2 cut(s) 146, 847
AfiI CCNNNNNNNGG 2 cut(s) 49, 922
AflIII ACRYGT 1 cut(s) 649
AgeI ACCGGT 1 cut(s) 307
AgsI TTSAA 4 cut(s) 209, 233, 290, 737
AluBI AGCT 5 cut(s) 416, 446, 523, 585, 742
AluI AGCT 5 cut(s) 416, 446, 523, 585, 742
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 3 cut(s) 266, 533, 568
AlwI GGATC 3 cut(s) 398, 411, 834
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 365, 520
ApoI RAATTY 1 cut(s) 62
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AseI ATTAAT 1 cut(s) 462
AsiGI ACCGGT 1 cut(s) 307
Asp700I GAANNNNTTC 1 cut(s) 204
AsuHPI GGTGA 1 cut(s) 274
BamHI GGATCC 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 78, 792
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 2 cut(s) 377, 532
BceAI ACGGC 1 cut(s) 67
BciVI GTATCC 1 cut(s) 559
BcoDI GTCTC 3 cut(s) 266, 533, 568
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 1 cut(s) 946
BfuI GTATCC 1 cut(s) 559
BglII AGATCT 1 cut(s) 222
BisI GCNGC 3 cut(s) 366, 369, 521
BlsI GCNGC 3 cut(s) 367, 370, 522
BmiI GGNNCC 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 914
BmsI GCATC 1 cut(s) 494
BmuI ACTGGG 1 cut(s) 914
BpiI GAAGAC 2 cut(s) 78, 792
BpmI CTGGAG 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 266, 568
BsaJI CCNNGG 4 cut(s) 271, 333, 666, 763
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 922
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 864, 920
Bse3DI GCAATG 1 cut(s) 889
BseDI CCNNGG 4 cut(s) 271, 333, 666, 763
BseLI CCNNNNNNNGG 2 cut(s) 49, 922
BseMI GCAATG 1 cut(s) 889
BseNI ACTGG 2 cut(s) 864, 920
BseRI GAGGAG 2 cut(s) 239, 780
BseXI GCAGC 2 cut(s) 377, 532
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 1 cut(s) 347
BshTI ACCGGT 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 49, 922
BsmAI GTCTC 3 cut(s) 266, 533, 568
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 1 cut(s) 347
Bso31I GGTCTC 2 cut(s) 266, 568
Bsp1286I GDGCHC 1 cut(s) 883
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 5 cut(s) 128, 222, 403, 430, 826
BspACI CCGC 2 cut(s) 369, 500
BspANI GGCC 1 cut(s) 347
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 3 cut(s) 398, 411, 834
BspTNI GGTCTC 2 cut(s) 266, 568
BsrDI GCAATG 1 cut(s) 889
BsrFI RCCGGY 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 2 cut(s) 864, 920
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 4 cut(s) 271, 333, 666, 763
BssMI GATC 5 cut(s) 128, 222, 403, 430, 826
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 666
Bst4CI ACNGT 4 cut(s) 275, 334, 544, 955
Bst6I CTCTTC 3 cut(s) 101, 271, 891
BstACI GRCGYC 1 cut(s) 381
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 518
BstDEI CTNAG 1 cut(s) 447
BstDSI CCRYGG 2 cut(s) 271, 333
BstFNI CGCG 1 cut(s) 651
BstKTI GATC 5 cut(s) 131, 225, 406, 433, 829
BstMAI GTCTC 3 cut(s) 266, 533, 568
BstMBI GATC 5 cut(s) 128, 222, 403, 430, 826
BstSFI CTRYAG 1 cut(s) 946
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 2 cut(s) 377, 532
BstV2I GAAGAC 2 cut(s) 78, 792
BstX2I RGATCY 2 cut(s) 222, 403
BstYI RGATCY 2 cut(s) 222, 403
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 2 cut(s) 271, 333
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 4 cut(s) 117, 426, 726, 927
Cac8I GCNNGC 1 cut(s) 518
CaiI CAGNNNCTG 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 307
Csp6I GTAC 2 cut(s) 145, 846
CspAI ACCGGT 1 cut(s) 307
CviAII CATG 2 cut(s) 570, 793
CviQI GTAC 2 cut(s) 145, 846
DdeI CTNAG 1 cut(s) 447
DpnI GATC 5 cut(s) 130, 224, 405, 432, 828
DpnII GATC 5 cut(s) 128, 222, 403, 430, 826
DraIII CACNNNGTG 2 cut(s) 795, 922
Eam1104I CTCTTC 3 cut(s) 101, 271, 891
EarI CTCTTC 3 cut(s) 101, 271, 891
Eco130I CCWWGG 1 cut(s) 666
Eco31I GGTCTC 2 cut(s) 266, 568
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 487
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 2 cut(s) 573, 796
FatI CATG 2 cut(s) 569, 792
Fnu4HI GCNGC 3 cut(s) 366, 369, 521
Fsp4HI GCNGC 3 cut(s) 366, 369, 521
FspBI CTAG 1 cut(s) 708
GluI GCNGC 3 cut(s) 366, 369, 521
GsuI CTGGAG 1 cut(s) 881
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 2 cut(s) 573, 796
HinfI GANTC 7 cut(s) 122, 374, 507, 598, 671, 770, 863
HpaII CCGG 1 cut(s) 308
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 3 cut(s) 379, 603, 831
Hpy188III TCNNGA 3 cut(s) 67, 126, 132
Hpy8I GTNNAC 1 cut(s) 724
Hpy99I CGWCG 1 cut(s) 386
HpyCH4III ACNGT 4 cut(s) 275, 334, 544, 955
HpyCH4IV ACGT 2 cut(s) 11, 381
HpyCH4V TGCA 7 cut(s) 320, 365, 478, 485, 520, 681, 731
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 2 cut(s) 11, 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 2 cut(s) 573, 796
Kzo9I GATC 5 cut(s) 128, 222, 403, 430, 826
LmnI GCTCC 2 cut(s) 413, 878
LpnPI CCDG 7 cut(s) 80, 321, 591, 828, 845, 923, 933
Lsp1109I GCAGC 2 cut(s) 377, 532
LweI GCATC 1 cut(s) 494
MaeI CTAG 1 cut(s) 708
MaeII ACGT 2 cut(s) 11, 381
MaeIII GTNAC 1 cut(s) 215
MalI GATC 5 cut(s) 130, 224, 405, 432, 828
MboI GATC 5 cut(s) 128, 222, 403, 430, 826
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 2 cut(s) 222, 403
MhlI GDGCHC 1 cut(s) 883
MluCI AATT 2 cut(s) 62, 732
MluI ACGCGT 1 cut(s) 649
MlyI GAGTC 3 cut(s) 116, 516, 872
MmeI TCCRAC 1 cut(s) 484
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 2 cut(s) 359, 462
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 308
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 1 cut(s) 502
MvnI CGCG 1 cut(s) 651
NdeII GATC 5 cut(s) 128, 222, 403, 430, 826
NlaIII CATG 2 cut(s) 573, 796
NlaIV GGNNCC 1 cut(s) 405
NmeAIII GCCGAG 1 cut(s) 790
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 487
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 4 cut(s) 374, 598, 671, 770
PflMI CCANNNNNTGG 1 cut(s) 922
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 3 cut(s) 367, 370, 522
PleI GAGTC 3 cut(s) 116, 515, 871
PpsI GAGTC 3 cut(s) 116, 515, 871
PshBI ATTAAT 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 405
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 2 cut(s) 222, 403
RsaI GTAC 2 cut(s) 146, 847
RsaNI GTAC 2 cut(s) 145, 846
SaqAI TTAA 2 cut(s) 359, 462
SatI GCNGC 3 cut(s) 366, 369, 521
Sau3AI GATC 5 cut(s) 128, 222, 403, 430, 826
SchI GAGTC 3 cut(s) 116, 516, 872
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 1 cut(s) 494
SfcI CTRYAG 1 cut(s) 946
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 2 cut(s) 62, 732
SsiI CCGC 2 cut(s) 369, 500
SspMI CTAG 1 cut(s) 708
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 4 cut(s) 275, 334, 544, 955
TaiI ACGT 2 cut(s) 14, 384
TaqI TCGA 2 cut(s) 442, 548
TasI AATT 2 cut(s) 62, 732
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 4 cut(s) 374, 598, 671, 770
Tru1I TTAA 2 cut(s) 359, 462
Tru9I TTAA 2 cut(s) 359, 462
TscAI CASTG 4 cut(s) 124, 433, 733, 927
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 2 cut(s) 365, 520
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 4 cut(s) 45, 509, 714, 878
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 4 cut(s) 124, 433, 733, 927
Van91I CCANNNNNTGG 1 cut(s) 922
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 708
ZraI GACGTC 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.