RLG00000000048

DNA-directed 5'-3' RNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0004622
Physical Location & Seq
Forward (+)
289049 .. 293273
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000048

Sequence Viewer

Length: 2898 bp
ATGGGCTACATCAAACTGGCATGCCCAGTAACCCACATTCAGAATGAGAAACTGTCATCCCATTCAATCAAATTGGGATGCCCCGGATCTGACATGTCTCTTAGTAGGAGTAAGAATAGCATGAAACTCAGAAGCTTCGATACATTTCGAAATCGAGAAATTTCTACTGGAGCAGGTGCTATCCGAGAACAATTAGCCGATCTGGATTTGAGAATTATTATAGATTATTCGCTGTTAGAATGGAAAGAATTGGGGGAAGAAGGGTCCACGGGGAATGAATGGGAAGATCGAAAGGTTGGAAGACGAAAGGATTTTTTGGTTAGACGCATGGAGTTGGCTAAGCATTTTATTCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTATTACCAGTTCTTCCGCCCGAGTTGAGACCAATCATTCAGATAGATGGGGGTAAACTAATGAGCTCGGATATTAATGAACTCTATAGAAGAGTTATTTATCGGAACAATACTCTTATCGATCTCTTAACAACAAGTAGATCTACGCCAGGAGAATTAGTAATGTGTCAGGAGAAATTGGTACAAGAAGCTGTGGATACACTTCTTGATAATGGAATCCGTGGACAACCAATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGTAATTGAAGGAAAAGAGGGAAGATTTCGTGAGACTCTGCTTGGCAAACGGGTCGATTATTCGGGGCGTTCTGTCATTGTTGTAGGTCCTTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATAGCAATAGAGCTTTTCCAGACATTTGTAATTCGTGGTTTAATTAGACAGCATTTTGCCTCGAATATAGGAGTGGCTAAGAGTAAAATTCGGGAAAAAGAGCCGGTTGTATGGGAAATACTTCAGGAAGTTATGCAAGGGCATCCCGTATTGCTGAATAGGGCGCCTACTCTGCATAGATTAGGCATACAGGCGTTCCAGCCCATTTTAGTAGAAGGACATGCTATTTGTTTACATCCATTAGTTTGTAAGGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAGCGGGAATCGCCGAGGTATTTGTGCAAATAGGTATAATCCATATAATCGCACAAATTCGAAAAATGAAAGAATTGCCGATAATAATTCTAAATATACGAAAGAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGGTGGCGGCTAGACCAACGTGTTATTACTTCAAGAGAAACTCCCATCGAAGTTCACTATGAGTCTTTGGGTACCTCTCATGAGATTTATGGACACTATGTAATAGTAAGAAGTATAAAAAAAGAAGTTCTTTGTATATATGTTCGAACCACAGTTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCTTCAGCTAGGACCACAGTTCCTGAATTTCTATGCGAATCAAGATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCAGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACCGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAAGTTTGATTTTGGAAAAACACCATCATTATGGTAATGTACACGCGGTAGAAAAATTACGCCAATCTATTGAGATATGGTATGCTACAAGTGAATATTTGCGACAAGAAATGAGTCCTAATTTTAGGATGACGGACCCTTTTAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACACATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGTCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTCGAAATAAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACGTCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGGTCATTCTCTTTCGGTTGAAGGACGATATTTTTCTAGTCTTTCAGTAA

Protein Analysis

966

Amino Acids

109.01

Weight (kDa)

9.09

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 1 - 233 3.7e-34 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 235 - 282 3.4e-07 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_2 PF00623 293 - 375 4.6e-26 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 380 - 640 3.3e-14 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 671 - 735 5.9e-14 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 750 - 936 2.5e-39 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
Acc65I GGTACC 1 cut(s) 1469
AccB1I GGYRCC 3 cut(s) 949, 1469, 2396
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccBSI CCGCTC 1 cut(s) 1218
AccII CGCG 3 cut(s) 783, 2012, 2506
AclWI GGATC 8 cut(s) 94, 1071, 1157, 1170, 2192, 2205, 2630, 2840
AcoI YGGCCR 1 cut(s) 2641
AcsI RAATTY 5 cut(s) 159, 873, 1270, 1640, 2448
AcuI CTGAAG 2 cut(s) 893, 1602
AcyI GRCGYC 1 cut(s) 950
AfiI CCNNNNNNNGG 5 cut(s) 1157, 1716, 1937, 2429, 2657
AflIII ACRYGT 2 cut(s) 93, 1417
AgeI ACCGGT 1 cut(s) 2753
AgsI TTSAA 8 cut(s) 66, 668, 1431, 2231, 2357, 2366, 2570, 2869
AjnI CCWGG 2 cut(s) 535, 2664
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 4 cut(s) 102, 409, 686, 1152
AlwI GGATC 8 cut(s) 94, 1071, 1157, 1170, 2192, 2205, 2630, 2840
AlwNI CAGNNNCTG 1 cut(s) 1637
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 3 cut(s) 1110, 1746, 2641
ApoI RAATTY 5 cut(s) 159, 873, 1270, 1640, 2448
ArsI GACNNNNNNTTYG 6 cut(s) 996, 1028, 1685, 1717, 2835, 2867
AseI ATTAAT 2 cut(s) 462, 1811
AsiGI ACCGGT 1 cut(s) 2753
Asp700I GAANNNNTTC 2 cut(s) 906, 2361
Asp718I GGTACC 1 cut(s) 1469
AspLEI GCGC 1 cut(s) 952
AspS9I GGNCC 7 cut(s) 264, 746, 1111, 1626, 1746, 2101, 2501
AsuC2I CCSGG 2 cut(s) 84, 2721
AsuII TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 5 cut(s) 264, 746, 1626, 2101, 2501
BaeGI GKGCMC 1 cut(s) 2726
BaeI ACNNNNGTAYC 2 cut(s) 2298, 2331
BamHI GGATCC 2 cut(s) 1162, 2197
BanI GGYRCC 3 cut(s) 949, 1469, 2396
BanII GRGCYC 4 cut(s) 455, 1203, 2290, 2651
BarI GAAGNNNNNNTAC 2 cut(s) 124, 156
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 455
BccI CCATC 6 cut(s) 370, 428, 1052, 1451, 1772, 1998
BceAI ACGGC 1 cut(s) 2501
BcgI CGANNNNNNTGC 4 cut(s) 332, 366, 694, 728
BciT130I CCWGG 2 cut(s) 537, 2666
BciVI GTATCC 2 cut(s) 577, 2299
BcnI CCSGG 2 cut(s) 84, 2721
BcoDI GTCTC 4 cut(s) 102, 409, 686, 1152
BfaI CTAG 6 cut(s) 1409, 1623, 1850, 1946, 2142, 2885
BfmI CTRYAG 3 cut(s) 472, 2685, 2778
BfoI RGCGCY 1 cut(s) 953
BfuAI ACCTGC 1 cut(s) 164
BfuI GTATCC 2 cut(s) 577, 2299
BglII AGATCT 1 cut(s) 527
BisI GCNGC 1 cut(s) 1406
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 1407
Bme1390I CCNGG 4 cut(s) 84, 537, 2666, 2721
Bme18I GGWCC 5 cut(s) 264, 746, 1626, 2101, 2501
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 7 cut(s) 264, 746, 1111, 1626, 1746, 2101, 2501
BmrFI CCNGG 4 cut(s) 84, 537, 2666, 2721
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 8 cut(s) 68, 937, 1333, 1945, 2162, 2317, 2427, 2498
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 2638, 2670
BpmI CTGGAG 2 cut(s) 189, 1134
Bpu1102I GCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
BpuEI CTTGAG 2 cut(s) 1086, 2142
BpuMI CCSGG 2 cut(s) 84, 2721
Bsa29I ATCGAT 4 cut(s) 507, 766, 1776, 2554
BsaAI YACGTR 2 cut(s) 2243, 2381
BsaBI GATNNNNATC 2 cut(s) 1062, 2837
BsaHI GRCGYC 1 cut(s) 950
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 6 cut(s) 82, 267, 607, 1228, 1594, 2404
BsaWI WCCGGW 2 cut(s) 1398, 2753
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 5 cut(s) 1157, 1716, 1937, 2429, 2657
Bse118I RCCGGY 2 cut(s) 889, 2753
Bse1I ACTGG 6 cut(s) 21, 26, 172, 395, 1749, 2114
Bse3DI GCAATG 1 cut(s) 2511
Bse8I GATNNNNATC 2 cut(s) 1062, 2837
BseBI CCWGG 2 cut(s) 537, 2666
BseCI ATCGAT 4 cut(s) 507, 766, 1776, 2554
BseDI CCNNGG 6 cut(s) 82, 267, 607, 1228, 1594, 2404
BseJI GATNNNNATC 2 cut(s) 1062, 2837
BseLI CCNNNNNNNGG 5 cut(s) 1157, 1716, 1937, 2429, 2657
BseMI GCAATG 1 cut(s) 2511
BseMII CTCAG 2 cut(s) 142, 1727
BseNI ACTGG 6 cut(s) 21, 26, 172, 395, 1749, 2114
BseSI GKGCMC 1 cut(s) 2726
BsgI GTGCAG 2 cut(s) 2817, 2828
Bsh1236I CGCG 3 cut(s) 783, 2012, 2506
BshFI GGCC 3 cut(s) 1112, 1748, 2643
BshNI GGYRCC 3 cut(s) 949, 1469, 2396
BshTI ACCGGT 1 cut(s) 2753
BshVI ATCGAT 4 cut(s) 507, 766, 1776, 2554
BsiHKAI GWGCWC 1 cut(s) 455
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 7 cut(s) 84, 890, 1399, 2441, 2644, 2720, 2754
BslFI GGGAC 2 cut(s) 638, 2797
BslI CCNNNNNNNGG 5 cut(s) 1157, 1716, 1937, 2429, 2657
BsmAI GTCTC 4 cut(s) 102, 409, 686, 1152
BsmFI GGGAC 2 cut(s) 638, 2797
BsnI GGCC 3 cut(s) 1112, 1748, 2643
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
Bsp1286I GDGCHC 5 cut(s) 455, 1203, 2290, 2651, 2726
Bsp1407I TGTACA 1 cut(s) 2005
Bsp1720I GCTNAGC 1 cut(s) 339
Bsp68I TCGCGA 1 cut(s) 783
BspANI GGCC 3 cut(s) 1112, 1748, 2643
BspCNI CTCAG 2 cut(s) 141, 1726
BspDI ATCGAT 4 cut(s) 507, 766, 1776, 2554
BspFNI CGCG 3 cut(s) 783, 2012, 2506
BspHI TCATGA 1 cut(s) 1477
BspMAI CTGCAG 1 cut(s) 2782
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 8 cut(s) 94, 1071, 1157, 1170, 2192, 2205, 2630, 2840
BspT104I TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
BspT107I GGYRCC 3 cut(s) 949, 1469, 2396
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 1218
BsrDI GCAATG 1 cut(s) 2511
BsrFI RCCGGY 2 cut(s) 889, 2753
BsrGI TGTACA 1 cut(s) 2005
BsrI ACTGG 6 cut(s) 21, 26, 172, 395, 1749, 2114
BssAI RCCGGY 2 cut(s) 889, 2753
BssECI CCNNGG 6 cut(s) 82, 267, 607, 1228, 1594, 2404
BssNI GRCGYC 1 cut(s) 950
BssT1I CCWWGG 1 cut(s) 1594
Bst2UI CCWGG 2 cut(s) 537, 2666
Bst4CI ACNGT 6 cut(s) 54, 629, 1552, 1633, 2401, 2851
Bst6I CTCTTC 2 cut(s) 472, 1621
BstACI GRCGYC 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 2005
BstBAI YACGTR 2 cut(s) 2243, 2381
BstBI TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
BstC8I GCNNGC 2 cut(s) 22, 2290
BstDEI CTNAG 6 cut(s) 101, 128, 339, 864, 1713, 1935
BstDSI CCRYGG 2 cut(s) 267, 607
BstFNI CGCG 3 cut(s) 783, 2012, 2506
BstH2I RGCGCY 1 cut(s) 953
BstHHI GCGC 1 cut(s) 952
BstMAI GTCTC 4 cut(s) 102, 409, 686, 1152
BstMWI GCNNNNNNNGC 6 cut(s) 958, 1198, 1224, 1352, 1402, 1745
BstNI CCWGG 2 cut(s) 537, 2666
BstNSI RCATGY 3 cut(s) 24, 97, 1010
BstSCI CCNGG 4 cut(s) 82, 535, 2664, 2719
BstSFI CTRYAG 3 cut(s) 472, 2685, 2778
BstSLI GKGCMC 1 cut(s) 2726
BstSNI TACGTA 1 cut(s) 2381
BstUI CGCG 3 cut(s) 783, 2012, 2506
BstV2I GAAGAC 1 cut(s) 307
BstX2I RGATCY 5 cut(s) 86, 527, 1162, 2197, 2622
BstXI CCANNNNNNTGG 1 cut(s) 1997
BstYI RGATCY 5 cut(s) 86, 527, 1162, 2197, 2622
Bsu15I ATCGAT 4 cut(s) 507, 766, 1776, 2554
BsuI GTATCC 2 cut(s) 577, 2299
BsuRI GGCC 3 cut(s) 1112, 1748, 2643
BsuTUI ATCGAT 4 cut(s) 507, 766, 1776, 2554
BtgI CCRYGG 2 cut(s) 267, 607
BtgZI GCGATG 1 cut(s) 2521
BtsIMutI CAGTG 2 cut(s) 1683, 2811
BtuMI TCGCGA 1 cut(s) 783
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 22, 2290
CaiI CAGNNNCTG 1 cut(s) 1637
CciI TCATGA 1 cut(s) 1477
CfoI GCGC 1 cut(s) 952
Cfr10I RCCGGY 2 cut(s) 889, 2753
Cfr13I GGNCC 7 cut(s) 264, 746, 1111, 1626, 1746, 2101, 2501
ClaI ATCGAT 4 cut(s) 507, 766, 1776, 2554
CseI GACGC 1 cut(s) 333
CsiI ACCWGGT 1 cut(s) 2664
CspAI ACCGGT 1 cut(s) 2753
CviAII CATG 9 cut(s) 21, 94, 121, 328, 1007, 1079, 1478, 2657, 2786
DdeI CTNAG 6 cut(s) 101, 128, 339, 864, 1713, 1935
DinI GGCGCC 1 cut(s) 951
EaeI YGGCCR 1 cut(s) 2641
Eam1104I CTCTTC 2 cut(s) 472, 1621
EarI CTCTTC 2 cut(s) 472, 1621
EciI GGCGGA 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 453
Eco105I TACGTA 1 cut(s) 2381
Eco130I CCWWGG 1 cut(s) 1594
Eco24I GRGCYC 4 cut(s) 455, 1203, 2290, 2651
Eco31I GGTCTC 1 cut(s) 409
Eco32I GATATC 1 cut(s) 2336
Eco47I GGWCC 5 cut(s) 264, 746, 1626, 2101, 2501
Eco53kI GAGCTC 1 cut(s) 453
Eco57I CTGAAG 2 cut(s) 893, 1602
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 746
EcoRII CCWGG 2 cut(s) 535, 2664
EcoRV GATATC 1 cut(s) 2336
EcoT14I CCWWGG 1 cut(s) 1594
EcoT22I ATGCAT 2 cut(s) 2155, 2513
EcoT38I GRGCYC 4 cut(s) 455, 1203, 2290, 2651
EgeI GGCGCC 1 cut(s) 951
EheI GGCGCC 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 1594
FaeI CATG 9 cut(s) 24, 97, 124, 331, 1010, 1082, 1481, 2660, 2789
FalI AAGNNNNNCTT 4 cut(s) 1176, 1208, 1512, 1544
FaqI GGGAC 2 cut(s) 638, 2797
FatI CATG 9 cut(s) 20, 93, 120, 327, 1006, 1078, 1477, 2656, 2785
FauI CCCGC 3 cut(s) 1211, 2297, 2692
FauNDI CATATG 2 cut(s) 1134, 2121
Fnu4HI GCNGC 1 cut(s) 1406
FriOI GRGCYC 4 cut(s) 455, 1203, 2290, 2651
Fsp4HI GCNGC 1 cut(s) 1406
FspBI CTAG 6 cut(s) 1409, 1623, 1850, 1946, 2142, 2885
GlaI GCGC 1 cut(s) 951
GluI GCNGC 1 cut(s) 1406
GsuI CTGGAG 2 cut(s) 189, 1134
HaeII RGCGCY 1 cut(s) 953
HaeIII GGCC 3 cut(s) 1112, 1748, 2643
HapII CCGG 7 cut(s) 84, 890, 1399, 2441, 2644, 2720, 2754
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 952
Hin1I GRCGYC 1 cut(s) 950
Hin1II CATG 9 cut(s) 24, 97, 124, 331, 1010, 1082, 1481, 2660, 2789
Hin6I GCGC 1 cut(s) 950
HinP1I GCGC 1 cut(s) 950
HindIII AAGCTT 1 cut(s) 133
HpaII CCGG 7 cut(s) 84, 890, 1399, 2441, 2644, 2720, 2754
Hpy166II GTNNAC 9 cut(s) 267, 443, 611, 643, 1019, 1118, 1453, 2008, 2164
Hpy8I GTNNAC 9 cut(s) 267, 443, 611, 643, 1019, 1118, 1453, 2008, 2164
Hpy99I CGWCG 1 cut(s) 2326
HpyAV CCTTC 8 cut(s) 254, 662, 759, 995, 1941, 2240, 2828, 2863
HpyCH4III ACNGT 6 cut(s) 54, 629, 1552, 1633, 2401, 2851
HpyCH4IV ACGT 5 cut(s) 1417, 2242, 2321, 2380, 2615
HpyCH4V TGCA 9 cut(s) 922, 961, 1241, 1346, 2153, 2511, 2780, 2798, 2809
HpyF10VI GCNNNNNNNGC 6 cut(s) 958, 1198, 1224, 1352, 1402, 1745
HpyF3I CTNAG 6 cut(s) 101, 128, 339, 864, 1713, 1935
HpySE526I ACGT 5 cut(s) 1417, 2242, 2321, 2380, 2615
Hsp92I GRCGYC 1 cut(s) 950
Hsp92II CATG 9 cut(s) 24, 97, 124, 331, 1010, 1082, 1481, 2660, 2789
HspAI GCGC 1 cut(s) 950
KasI GGCGCC 1 cut(s) 949
KpnI GGTACC 1 cut(s) 1473
LmnI GCTCC 6 cut(s) 170, 1352, 1401, 2138, 2285, 2646
LweI GCATC 8 cut(s) 68, 937, 1333, 1945, 2162, 2317, 2427, 2498
MabI ACCWGGT 1 cut(s) 2664
MaeI CTAG 6 cut(s) 1409, 1623, 1850, 1946, 2142, 2885
MaeII ACGT 5 cut(s) 1417, 2242, 2321, 2380, 2615
MaeIII GTNAC 2 cut(s) 28, 2279
MbiI CCGCTC 1 cut(s) 1218
MboII GAAGA 8 cut(s) 269, 296, 312, 392, 489, 693, 1595, 1608
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 5 cut(s) 86, 527, 1162, 2197, 2622
MhlI GDGCHC 5 cut(s) 455, 1203, 2290, 2651, 2726
Mly113I GGCGCC 1 cut(s) 950
MlyI GAGTC 7 cut(s) 688, 1380, 1469, 1682, 1858, 2089, 2428
MmeI TCCRAC 3 cut(s) 277, 2175, 2304
Mph1103I ATGCAT 2 cut(s) 2155, 2513
MroXI GAANNNNTTC 2 cut(s) 906, 2361
MslI CAYNNNNRTG 3 cut(s) 768, 1995, 2754
MspA1I CMGCKG 1 cut(s) 1717
MspI CCGG 7 cut(s) 84, 890, 1399, 2441, 2644, 2720, 2754
MspR9I CCNGG 4 cut(s) 84, 537, 2666, 2721
MunI CAATTG 1 cut(s) 1347
MvaI CCWGG 2 cut(s) 537, 2666
MvnI CGCG 3 cut(s) 783, 2012, 2506
MwoI GCNNNNNNNGC 6 cut(s) 958, 1198, 1224, 1352, 1402, 1745
NarI GGCGCC 1 cut(s) 950
NciI CCSGG 2 cut(s) 84, 2721
NdeI CATATG 2 cut(s) 1134, 2121
NlaIII CATG 9 cut(s) 24, 97, 124, 331, 1010, 1082, 1481, 2660, 2789
NmeAIII GCCGAG 2 cut(s) 1253, 1631
NruI TCGCGA 1 cut(s) 783
NsiI ATGCAT 2 cut(s) 2155, 2513
NspI RCATGY 3 cut(s) 24, 97, 1010
NspV TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
PaeI GCATGC 1 cut(s) 24
PagI TCATGA 1 cut(s) 1477
PaqCI CACCTGC 1 cut(s) 164
PciI ACATGT 1 cut(s) 93
PdmI GAANNNNTTC 2 cut(s) 906, 2361
PfeI GAWTC 6 cut(s) 603, 1138, 1222, 1652, 2556, 2801
PflFI GACNNNGTC 1 cut(s) 2666
PflMI CCANNNNNTGG 1 cut(s) 1157
PinAI ACCGGT 1 cut(s) 2753
PkrI GCNGC 1 cut(s) 1407
PleI GAGTC 7 cut(s) 688, 1379, 1468, 1682, 1858, 2088, 2427
PluTI GGCGCC 1 cut(s) 953
PpsI GAGTC 7 cut(s) 688, 1379, 1468, 1682, 1858, 2088, 2427
Ppu21I YACGTR 2 cut(s) 2243, 2381
PpuMI RGGWCCY 1 cut(s) 746
PscI ACATGT 1 cut(s) 93
PshBI ATTAAT 2 cut(s) 462, 1811
Psp124BI GAGCTC 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 746
Psp6I CCWGG 2 cut(s) 535, 2664
PspGI CCWGG 2 cut(s) 535, 2664
PspPI GGNCC 7 cut(s) 264, 746, 1111, 1626, 1746, 2101, 2501
PspPPI RGGWCCY 1 cut(s) 746
PsrI GAACNNNNNNTAC 4 cut(s) 2584, 2616, 2734, 2766
PstI CTGCAG 1 cut(s) 2782
PstNI CAGNNNCTG 1 cut(s) 1637
PsuI RGATCY 5 cut(s) 86, 527, 1162, 2197, 2622
PsyI GACNNNGTC 1 cut(s) 2666
RruI TCGCGA 1 cut(s) 783
RseI CAYNNNNRTG 3 cut(s) 768, 1995, 2754
SacI GAGCTC 1 cut(s) 455
SatI GCNGC 1 cut(s) 1406
Sau96I GGNCC 7 cut(s) 264, 746, 1111, 1626, 1746, 2101, 2501
SchI GAGTC 7 cut(s) 688, 1380, 1469, 1682, 1858, 2089, 2428
ScrFI CCNGG 4 cut(s) 84, 537, 2666, 2721
SduI GDGCHC 5 cut(s) 455, 1203, 2290, 2651, 2726
SexAI ACCWGGT 1 cut(s) 2664
SfaNI GCATC 8 cut(s) 68, 937, 1333, 1945, 2162, 2317, 2427, 2498
SfcI CTRYAG 3 cut(s) 472, 2685, 2778
SfoI GGCGCC 1 cut(s) 951
SfuI TTCGAA 6 cut(s) 148, 352, 1274, 1543, 2521, 2590
SinI GGWCC 5 cut(s) 264, 746, 1626, 2101, 2501
SmiMI CAYNNNNRTG 3 cut(s) 768, 1995, 2754
SmlI CTYRAG 2 cut(s) 1101, 2157
SmoI CTYRAG 2 cut(s) 1101, 2157
SnaBI TACGTA 1 cut(s) 2381
SphI GCATGC 1 cut(s) 24
SspDI GGCGCC 1 cut(s) 949
SspI AATATT 1 cut(s) 2063
SspMI CTAG 6 cut(s) 1409, 1623, 1850, 1946, 2142, 2885
SstI GAGCTC 1 cut(s) 455
StyD4I CCNGG 4 cut(s) 82, 535, 2664, 2719
StyI CCWWGG 1 cut(s) 1594
TaaI ACNGT 6 cut(s) 54, 629, 1552, 1633, 2401, 2851
TaiI ACGT 5 cut(s) 1420, 2245, 2324, 2383, 2618
TatI WGTACW 2 cut(s) 2005, 2161
TauI GCSGC 1 cut(s) 1408
TfiI GAWTC 6 cut(s) 603, 1138, 1222, 1652, 2556, 2801
TscAI CASTG 2 cut(s) 1690, 2811
TspGWI ACGGA 4 cut(s) 596, 1162, 2114, 2298
TspRI CASTG 2 cut(s) 1690, 2811
Tth111I GACNNNGTC 1 cut(s) 2666
Van91I CCANNNNNTGG 1 cut(s) 1157
VpaK11BI GGWCC 5 cut(s) 264, 746, 1626, 2101, 2501
VspI ATTAAT 2 cut(s) 462, 1811
XapI RAATTY 5 cut(s) 159, 873, 1270, 1640, 2448
XceI RCATGY 3 cut(s) 24, 97, 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 2465
XmnI GAANNNNTTC 2 cut(s) 906, 2361
XspI CTAG 6 cut(s) 1409, 1623, 1850, 1946, 2142, 2885
Zsp2I ATGCAT 2 cut(s) 2155, 2513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.