RLG00000000117

DNA-directed 5'-3' RNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0004622
Physical Location & Seq
Forward (+)
587662 .. 590686
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000117

Sequence Viewer

Length: 2856 bp
ATGGAGTTGGCTAAGCATTTTATTCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTATTACCAGTTCTTCCGCCCGAGTTGAGACCAATCATTCAGATAGATGGGGGTAAACTAATGAGCTCGGATATTAATGAACTCTATAGAAGAGTTATTTATCGGAACAATACTCTTATCGATCTCTTAACAACAAGTAGATCTACGCCAGGAGAATTAGTAATGTGTCAGGAGAAATTGGTACAAGAAGCTGTGGATACACTTCTTGATAATGGAATCCGTGGACAACCAATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGTAATTGAAGGAAAAGAGGGAAGATTTCGTGAGACTCTGCTTGGCAAACGGGTCGATTATTCGGGGCGTTCTGTCATTGTTGTAGGTCCTTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATAGCAATAGAGCTTTTCCAGACATTTGTAATTCGTGGTTTAATTAGACAGCATTTTGCCTCGAATATAGGAGTGGCTAAGAGTAAAATTCGGGAAAAAGAGCCGGTTGTATGGGAAATACTTCAGGAAGTTATGCAAGGGCATCCCGTATTGCTGAATAGGGCGCCTACTCTGCATAGATTAGGCATACAGGCGTTCCAGCCCATTTTAGTAGAAGGACATGCTATTTGTTTACATCCATTAGTTTGTAAGGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAGCGGGAATCGCCGAGGTATTTGTGCAAATAGGTATAATCCATATAATCGCACAAATTCGAAAAATGAAAGAATTGCCGATAATAATTCTAAATATACGAAAGAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGGTGGCGGCTAGACCAACGTGTTATTACTTCAAGAGAAACTCCCATCGAAGTTCACTATGAGTCTTTGGGTACCTCTCATGAGATTTATGGACACTATGTAATATTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCGTATGGTACCTAATCATATCGTATCGAAGCTAACTAGGACCACAGTTCCTGAATTTCTATGCGAATCAAGATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCAGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACCGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAAGTTTGATTTTGGAAAAACACCATCATTATGGTAATGTACACGCGGTAGAAAAATTACGCCAATCTATTGAGATATGGTATGCTACAAGTGAATATTTGCGACAAGAAATGAGTCCTAATTTTAGGATGACGGACCCTTTTAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACACATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGTCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTCGAAATAAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACGTCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGTACGAGCCCCCTCTAATGGAAAAATAAAATTCAATGAGGATTTGGTTCATCCCACACGTACACGTCATGGGCATCCTGCTTTTCTATGTTATATAGACTTGTATGTAACTATTGAAAGTGAAAATATTATACATAACGTGACTATTCCACCAAAAAGTTTGATTTTAGTGCAAAATGACCAATACGTAGAATCAGAGCAAGTGGTTGCTGAGATTCGCGCGGGAGCATACACTTTTAATTTTAAAGAGAGGGTTCGAAAACATATTTATTCTGACTCAGAGGGAGAAATGCACTGGAGTACCGATGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGGTCATTCTCTTTCGGTTGAAGGACGATATTTTTCTAGTCTTTCAGTAA

Protein Analysis

952

Amino Acids

107.55

Weight (kDa)

8.98

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 8 - 124 2.6e-30 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 126 - 173 3.3e-07 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_2 PF00623 184 - 266 4.5e-26 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 271 - 524 1.6e-14 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 555 - 619 5.8e-14 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 634 - 827 1.1e-39 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1142, 1246
AccB1I GGYRCC 4 cut(s) 622, 1142, 1246, 2048
AccB7I CCANNNNNTGG 1 cut(s) 830
AccBSI CCGCTC 1 cut(s) 891
AccII CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
AclWI GGATC 7 cut(s) 744, 830, 843, 1844, 1857, 2282, 2798
AcoI YGGCCR 1 cut(s) 2293
AcsI RAATTY 5 cut(s) 546, 943, 1292, 2100, 2468
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 1 cut(s) 623
AfiI CCNNNNNNNGG 6 cut(s) 830, 1368, 1589, 2081, 2309, 2456
AflIII ACRYGT 4 cut(s) 1090, 2437, 2495, 2501
AgeI ACCGGT 1 cut(s) 2405
AgsI TTSAA 9 cut(s) 341, 1104, 1883, 2009, 2018, 2222, 2473, 2555, 2827
AjiI CACGTC 1 cut(s) 2504
AjnI CCWGG 2 cut(s) 208, 2316
AluBI AGCT 9 cut(s) 126, 251, 472, 827, 1028, 1213, 1270, 1793, 2336
AluI AGCT 9 cut(s) 126, 251, 472, 827, 1028, 1213, 1270, 1793, 2336
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 3 cut(s) 82, 359, 825
AlwI GGATC 7 cut(s) 744, 830, 843, 1844, 1857, 2282, 2798
AlwNI CAGNNNCTG 1 cut(s) 1289
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 783, 1398, 2293
ApoI RAATTY 5 cut(s) 546, 943, 1292, 2100, 2468
ArsI GACNNNNNNTTYG 6 cut(s) 669, 701, 1337, 1369, 2793, 2825
AseI ATTAAT 2 cut(s) 135, 1463
AsiGI ACCGGT 1 cut(s) 2405
Asp700I GAANNNNTTC 2 cut(s) 579, 2013
Asp718I GGTACC 2 cut(s) 1142, 1246
AspLEI GCGC 2 cut(s) 625, 2660
AspS9I GGNCC 6 cut(s) 419, 784, 1278, 1398, 1753, 2153
AsuC2I CCSGG 1 cut(s) 2373
AsuII TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 4 cut(s) 419, 1278, 1753, 2153
BaeGI GKGCMC 1 cut(s) 2378
BaeI ACNNNNGTAYC 4 cut(s) 1950, 1983, 2722, 2755
BamHI GGATCC 2 cut(s) 835, 1849
BanI GGYRCC 4 cut(s) 622, 1142, 1246, 2048
BanII GRGCYC 5 cut(s) 128, 876, 1942, 2303, 2449
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 940
BccI CCATC 6 cut(s) 43, 101, 725, 1124, 1424, 1650
BceAI ACGGC 1 cut(s) 2153
BcgI CGANNNNNNTGC 4 cut(s) 5, 39, 367, 401
BciT130I CCWGG 2 cut(s) 210, 2318
BciVI GTATCC 2 cut(s) 250, 1951
BcnI CCSGG 1 cut(s) 2373
BcoDI GTCTC 3 cut(s) 82, 359, 825
BfaI CTAG 6 cut(s) 1082, 1275, 1502, 1598, 1794, 2843
BfmI CTRYAG 3 cut(s) 145, 2337, 2430
BfoI RGCGCY 1 cut(s) 626
BfuI GTATCC 2 cut(s) 250, 1951
BglII AGATCT 1 cut(s) 200
BisI GCNGC 1 cut(s) 1079
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 1080
Bme1390I CCNGG 3 cut(s) 210, 2318, 2373
Bme18I GGWCC 4 cut(s) 419, 1278, 1753, 2153
BmeT110I CYCGRG 1 cut(s) 80
BmgBI CACGTC 1 cut(s) 2504
BmgT120I GGNCC 6 cut(s) 419, 784, 1278, 1398, 1753, 2153
BmrFI CCNGG 3 cut(s) 210, 2318, 2373
BmsI GCATC 8 cut(s) 610, 1006, 1597, 1814, 1969, 2079, 2150, 2521
BplI GAGNNNNNCTC 2 cut(s) 2290, 2322
BpmI CTGGAG 2 cut(s) 807, 2755
Bpu1102I GCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
BpuEI CTTGAG 2 cut(s) 759, 1794
BpuMI CCSGG 1 cut(s) 2373
Bsa29I ATCGAT 4 cut(s) 180, 439, 1428, 2206
BsaAI YACGTR 4 cut(s) 1895, 2033, 2498, 2626
BsaBI GATNNNNATC 2 cut(s) 735, 2795
BsaHI GRCGYC 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 280, 901, 1218, 2056
BsaWI WCCGGW 2 cut(s) 1071, 2405
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 6 cut(s) 830, 1368, 1589, 2081, 2309, 2456
Bse118I RCCGGY 2 cut(s) 562, 2405
Bse1I ACTGG 4 cut(s) 68, 1401, 1766, 2738
Bse3DI GCAATG 1 cut(s) 2163
Bse8I GATNNNNATC 2 cut(s) 735, 2795
BseBI CCWGG 2 cut(s) 210, 2318
BseCI ATCGAT 4 cut(s) 180, 439, 1428, 2206
BseDI CCNNGG 4 cut(s) 280, 901, 1218, 2056
BseJI GATNNNNATC 2 cut(s) 735, 2795
BseLI CCNNNNNNNGG 6 cut(s) 830, 1368, 1589, 2081, 2309, 2456
BseMI GCAATG 1 cut(s) 2163
BseMII CTCAG 3 cut(s) 1379, 2640, 2730
BseNI ACTGG 4 cut(s) 68, 1401, 1766, 2738
BseSI GKGCMC 1 cut(s) 2378
BsgI GTGCAG 2 cut(s) 2775, 2786
Bsh1236I CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
BshFI GGCC 3 cut(s) 785, 1400, 2295
BshNI GGYRCC 4 cut(s) 622, 1142, 1246, 2048
BshTI ACCGGT 1 cut(s) 2405
BshVI ATCGAT 4 cut(s) 180, 439, 1428, 2206
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 6 cut(s) 563, 1072, 2093, 2296, 2372, 2406
BslFI GGGAC 2 cut(s) 311, 2755
BslI CCNNNNNNNGG 6 cut(s) 830, 1368, 1589, 2081, 2309, 2456
BsmAI GTCTC 3 cut(s) 82, 359, 825
BsmFI GGGAC 2 cut(s) 311, 2755
BsnI GGCC 3 cut(s) 785, 1400, 2295
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 80
Bsp119I TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
Bsp1286I GDGCHC 6 cut(s) 128, 876, 1942, 2303, 2378, 2449
Bsp1407I TGTACA 1 cut(s) 1657
Bsp1720I GCTNAGC 1 cut(s) 12
Bsp68I TCGCGA 1 cut(s) 456
BspANI GGCC 3 cut(s) 785, 1400, 2295
BspCNI CTCAG 3 cut(s) 1378, 2641, 2729
BspDI ATCGAT 4 cut(s) 180, 439, 1428, 2206
BspFNI CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
BspHI TCATGA 1 cut(s) 1150
BspMAI CTGCAG 1 cut(s) 2434
BspPI GGATC 7 cut(s) 744, 830, 843, 1844, 1857, 2282, 2798
BspT104I TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
BspT107I GGYRCC 4 cut(s) 622, 1142, 1246, 2048
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 2163
BsrFI RCCGGY 2 cut(s) 562, 2405
BsrGI TGTACA 1 cut(s) 1657
BsrI ACTGG 4 cut(s) 68, 1401, 1766, 2738
BssAI RCCGGY 2 cut(s) 562, 2405
BssECI CCNNGG 4 cut(s) 280, 901, 1218, 2056
BssNI GRCGYC 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 1218
Bst2UI CCWGG 2 cut(s) 210, 2318
Bst4CI ACNGT 4 cut(s) 302, 1285, 2053, 2809
Bst6I CTCTTC 1 cut(s) 145
BstACI GRCGYC 1 cut(s) 623
BstAUI TGTACA 1 cut(s) 1657
BstBAI YACGTR 4 cut(s) 1895, 2033, 2498, 2626
BstBI TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
BstC8I GCNNGC 1 cut(s) 1942
BstDEI CTNAG 6 cut(s) 12, 537, 1365, 1587, 2649, 2716
BstDSI CCRYGG 1 cut(s) 280
BstFNI CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 625, 2660
BstMAI GTCTC 3 cut(s) 82, 359, 825
BstMWI GCNNNNNNNGC 6 cut(s) 631, 871, 897, 1025, 1075, 1397
BstNI CCWGG 2 cut(s) 210, 2318
BstNSI RCATGY 2 cut(s) 683, 2441
BstSCI CCNGG 3 cut(s) 208, 2316, 2371
BstSFI CTRYAG 3 cut(s) 145, 2337, 2430
BstSLI GKGCMC 1 cut(s) 2378
BstSNI TACGTA 2 cut(s) 2033, 2626
BstUI CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
BstX2I RGATCY 4 cut(s) 200, 835, 1849, 2274
BstXI CCANNNNNNTGG 1 cut(s) 1649
BstYI RGATCY 4 cut(s) 200, 835, 1849, 2274
Bsu15I ATCGAT 4 cut(s) 180, 439, 1428, 2206
BsuI GTATCC 2 cut(s) 250, 1951
BsuRI GGCC 3 cut(s) 785, 1400, 2295
BsuTUI ATCGAT 4 cut(s) 180, 439, 1428, 2206
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 2173
BtrI CACGTC 1 cut(s) 2504
BtsIMutI CAGTG 3 cut(s) 1335, 2731, 2769
BtuMI TCGCGA 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 1942
CaiI CAGNNNCTG 1 cut(s) 1289
CciI TCATGA 1 cut(s) 1150
CfoI GCGC 2 cut(s) 625, 2660
Cfr10I RCCGGY 2 cut(s) 562, 2405
Cfr13I GGNCC 6 cut(s) 419, 784, 1278, 1398, 1753, 2153
ClaI ATCGAT 4 cut(s) 180, 439, 1428, 2206
CsiI ACCWGGT 1 cut(s) 2316
CspAI ACCGGT 1 cut(s) 2405
CviAII CATG 6 cut(s) 680, 752, 1151, 2309, 2438, 2507
DdeI CTNAG 6 cut(s) 12, 537, 1365, 1587, 2649, 2716
DinI GGCGCC 1 cut(s) 624
DraI TTTAAA 1 cut(s) 2683
EaeI YGGCCR 1 cut(s) 2293
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
EciI GGCGGA 1 cut(s) 66
Ecl136II GAGCTC 1 cut(s) 126
Eco105I TACGTA 2 cut(s) 2033, 2626
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 5 cut(s) 128, 876, 1942, 2303, 2449
Eco31I GGTCTC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 1988
Eco47I GGWCC 4 cut(s) 419, 1278, 1753, 2153
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 208, 2316
EcoRV GATATC 1 cut(s) 1988
EcoT14I CCWWGG 1 cut(s) 1218
EcoT22I ATGCAT 2 cut(s) 1807, 2165
EcoT38I GRGCYC 5 cut(s) 128, 876, 1942, 2303, 2449
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 1218
FaeI CATG 6 cut(s) 683, 755, 1154, 2312, 2441, 2510
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 2 cut(s) 311, 2755
FatI CATG 6 cut(s) 679, 751, 1150, 2308, 2437, 2506
FauI CCCGC 4 cut(s) 884, 1949, 2344, 2653
FauNDI CATATG 2 cut(s) 807, 1773
Fnu4HI GCNGC 1 cut(s) 1079
FriOI GRGCYC 5 cut(s) 128, 876, 1942, 2303, 2449
Fsp4HI GCNGC 1 cut(s) 1079
FspBI CTAG 6 cut(s) 1082, 1275, 1502, 1598, 1794, 2843
GlaI GCGC 2 cut(s) 624, 2659
GluI GCNGC 1 cut(s) 1079
GsuI CTGGAG 2 cut(s) 807, 2755
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 3 cut(s) 785, 1400, 2295
HapII CCGG 6 cut(s) 563, 1072, 2093, 2296, 2372, 2406
HhaI GCGC 2 cut(s) 625, 2660
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 6 cut(s) 683, 755, 1154, 2312, 2441, 2510
Hin6I GCGC 2 cut(s) 623, 2658
HinP1I GCGC 2 cut(s) 623, 2658
HpaII CCGG 6 cut(s) 563, 1072, 2093, 2296, 2372, 2406
Hpy166II GTNNAC 9 cut(s) 116, 284, 316, 692, 791, 1126, 1660, 1816, 2501
Hpy8I GTNNAC 9 cut(s) 116, 284, 316, 692, 791, 1126, 1660, 1816, 2501
Hpy99I CGWCG 1 cut(s) 1978
HpyAV CCTTC 7 cut(s) 335, 432, 668, 1593, 1892, 2786, 2821
HpyCH4III ACNGT 4 cut(s) 302, 1285, 2053, 2809
HpyCH4IV ACGT 9 cut(s) 1090, 1894, 1973, 2032, 2267, 2497, 2503, 2577, 2625
HpyF10VI GCNNNNNNNGC 6 cut(s) 631, 871, 897, 1025, 1075, 1397
HpyF3I CTNAG 6 cut(s) 12, 537, 1365, 1587, 2649, 2716
HpySE526I ACGT 9 cut(s) 1090, 1894, 1973, 2032, 2267, 2497, 2503, 2577, 2625
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 6 cut(s) 683, 755, 1154, 2312, 2441, 2510
HspAI GCGC 2 cut(s) 623, 2658
KasI GGCGCC 1 cut(s) 622
KpnI GGTACC 2 cut(s) 1146, 1250
LmnI GCTCC 6 cut(s) 1025, 1074, 1790, 1937, 2298, 2663
LweI GCATC 8 cut(s) 610, 1006, 1597, 1814, 1969, 2079, 2150, 2521
MabI ACCWGGT 1 cut(s) 2316
MaeI CTAG 6 cut(s) 1082, 1275, 1502, 1598, 1794, 2843
MaeII ACGT 9 cut(s) 1090, 1894, 1973, 2032, 2267, 2497, 2503, 2577, 2625
MaeIII GTNAC 3 cut(s) 1931, 2545, 2578
MbiI CCGCTC 1 cut(s) 891
MboII GAAGA 4 cut(s) 65, 162, 366, 1219
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 4 cut(s) 200, 835, 1849, 2274
MhlI GDGCHC 6 cut(s) 128, 876, 1942, 2303, 2378, 2449
Mly113I GGCGCC 1 cut(s) 623
MlyI GAGTC 8 cut(s) 361, 1053, 1142, 1334, 1510, 1741, 2080, 2708
MmeI TCCRAC 2 cut(s) 1827, 1956
Mph1103I ATGCAT 2 cut(s) 1807, 2165
MroXI GAANNNNTTC 2 cut(s) 579, 2013
MslI CAYNNNNRTG 3 cut(s) 441, 1647, 2406
MspA1I CMGCKG 1 cut(s) 1369
MspI CCGG 6 cut(s) 563, 1072, 2093, 2296, 2372, 2406
MspR9I CCNGG 3 cut(s) 210, 2318, 2373
MunI CAATTG 1 cut(s) 1020
MvaI CCWGG 2 cut(s) 210, 2318
MvnI CGCG 5 cut(s) 456, 1664, 2158, 2658, 2660
MwoI GCNNNNNNNGC 6 cut(s) 631, 871, 897, 1025, 1075, 1397
NarI GGCGCC 1 cut(s) 623
NciI CCSGG 1 cut(s) 2373
NdeI CATATG 2 cut(s) 807, 1773
NlaIII CATG 6 cut(s) 683, 755, 1154, 2312, 2441, 2510
NmeAIII GCCGAG 2 cut(s) 926, 1255
NmuCI GTSAC 1 cut(s) 2578
NruI TCGCGA 1 cut(s) 456
NsiI ATGCAT 2 cut(s) 1807, 2165
NspI RCATGY 2 cut(s) 683, 2441
NspV TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
PagI TCATGA 1 cut(s) 1150
PciI ACATGT 1 cut(s) 2437
PdmI GAANNNNTTC 2 cut(s) 579, 2013
PfeI GAWTC 8 cut(s) 276, 811, 895, 1304, 2208, 2630, 2653, 2759
PflFI GACNNNGTC 1 cut(s) 2318
PflMI CCANNNNNTGG 1 cut(s) 830
PinAI ACCGGT 1 cut(s) 2405
PkrI GCNGC 1 cut(s) 1080
PleI GAGTC 8 cut(s) 361, 1052, 1141, 1334, 1510, 1740, 2079, 2708
PluTI GGCGCC 1 cut(s) 626
PpsI GAGTC 8 cut(s) 361, 1052, 1141, 1334, 1510, 1740, 2079, 2708
Ppu21I YACGTR 4 cut(s) 1895, 2033, 2498, 2626
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 2437
PshBI ATTAAT 2 cut(s) 135, 1463
Psp124BI GAGCTC 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 208, 2316
PspGI CCWGG 2 cut(s) 208, 2316
PspPI GGNCC 6 cut(s) 419, 784, 1278, 1398, 1753, 2153
PspPPI RGGWCCY 1 cut(s) 419
PsrI GAACNNNNNNTAC 4 cut(s) 2236, 2268, 2386, 2418
PstI CTGCAG 1 cut(s) 2434
PstNI CAGNNNCTG 1 cut(s) 1289
PsuI RGATCY 4 cut(s) 200, 835, 1849, 2274
PsyI GACNNNGTC 1 cut(s) 2318
RruI TCGCGA 1 cut(s) 456
RseI CAYNNNNRTG 3 cut(s) 441, 1647, 2406
SacI GAGCTC 1 cut(s) 128
SatI GCNGC 1 cut(s) 1079
Sau96I GGNCC 6 cut(s) 419, 784, 1278, 1398, 1753, 2153
SchI GAGTC 8 cut(s) 361, 1053, 1142, 1334, 1510, 1741, 2080, 2708
ScrFI CCNGG 3 cut(s) 210, 2318, 2373
SduI GDGCHC 6 cut(s) 128, 876, 1942, 2303, 2378, 2449
SexAI ACCWGGT 1 cut(s) 2316
SfaNI GCATC 8 cut(s) 610, 1006, 1597, 1814, 1969, 2079, 2150, 2521
SfcI CTRYAG 3 cut(s) 145, 2337, 2430
SfoI GGCGCC 1 cut(s) 624
SfuI TTCGAA 5 cut(s) 25, 947, 2173, 2242, 2695
SinI GGWCC 4 cut(s) 419, 1278, 1753, 2153
SmiMI CAYNNNNRTG 3 cut(s) 441, 1647, 2406
SmlI CTYRAG 2 cut(s) 774, 1809
SmoI CTYRAG 2 cut(s) 774, 1809
SnaBI TACGTA 2 cut(s) 2033, 2626
SspDI GGCGCC 1 cut(s) 622
SspI AATATT 3 cut(s) 1176, 1715, 2566
SspMI CTAG 6 cut(s) 1082, 1275, 1502, 1598, 1794, 2843
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 3 cut(s) 208, 2316, 2371
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 4 cut(s) 302, 1285, 2053, 2809
TaiI ACGT 9 cut(s) 1093, 1897, 1976, 2035, 2270, 2500, 2506, 2580, 2628
TatI WGTACW 2 cut(s) 1657, 1813
TauI GCSGC 1 cut(s) 1081
TfiI GAWTC 8 cut(s) 276, 811, 895, 1304, 2208, 2630, 2653, 2759
TscAI CASTG 3 cut(s) 1342, 2738, 2769
TseFI GTSAC 1 cut(s) 2578
Tsp45I GTSAC 1 cut(s) 2578
TspDTI ATGAA 9 cut(s) 153, 420, 740, 824, 969, 1542, 2200, 2390, 2477
TspGWI ACGGA 4 cut(s) 269, 835, 1766, 1950
TspRI CASTG 3 cut(s) 1342, 2738, 2769
Tth111I GACNNNGTC 1 cut(s) 2318
Van91I CCANNNNNTGG 1 cut(s) 830
VpaK11BI GGWCC 4 cut(s) 419, 1278, 1753, 2153
VspI ATTAAT 2 cut(s) 135, 1463
XapI RAATTY 5 cut(s) 546, 943, 1292, 2100, 2468
XceI RCATGY 2 cut(s) 683, 2441
XcmI CCANNNNNNNNNTGG 1 cut(s) 2117
XmnI GAANNNNTTC 2 cut(s) 579, 2013
XspI CTAG 6 cut(s) 1082, 1275, 1502, 1598, 1794, 2843
Zsp2I ATGCAT 2 cut(s) 1807, 2165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.