RLG00000000317

DNA-directed 5'-3' RNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004622
Physical Location & Seq
Reverse (-)
539152 .. 542684
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000317

Sequence Viewer

Length: 2799 bp
ATGAACTGGTGCTTGATCTTCTTTGGCAACTTGAGCTTCGATACATTTCGAAATCGAGAAATTTCTACTGGAGCAGGTGCTATCCGAGAACAATTAGCCGATCTGGATTTGAGAATTATTATAGATTATTCGCTGTTAGAATGGAAAGAATTGGGGGAAGAAGGGTCCACGGGGAATGAATGGGAAGATCGAAAGGTTGGAAGACGAAAGGATTTTTTGGTTAGACGCATGGAGTTGGCTAAGCATTTTATTCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTATTACCAGTTCTTCCGCCCGAGTTGAGACCAATCATTCAGATAGATGGGGGTAAACTAATGAGCTCGGATATTAATGAACTCTATAGAAGAGTTATTTATCGGAACAATACTCTTATCGATCTCTTAACAACAAGTAGATCTACGCCAGGAGAATTAGTAATGTGTCAGGAGAAATTGGTACAAGAAGCTGTGGATACACTTCTTGATAATGGAATCCGTGGACAACCAATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGTAATTGAAGGAAAAGAGGGAAGATTTCGTGAGACTCTGCTTGGCAAACGGGTCGATTATTCGGGGCGTTCTGTCATTGTTGTAGGTCCTTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATAGCAATAGAGCTTTTCCAGACATTTGTAATTCGTGGTTTAATTAGACAGCATTTTGCCTCGAATATAGGAGTGGCTAAGAGTAAAATTCGGGAAAAAGAGCCGGTTGTATGGGAAATACTTCAGGAAGTTATGCAAGGGCATCCCGTATTGCTGAATAGGGCGCCTACTCTGCATAGATTAGGCATACAGGCGTTCCAGCCCATTTTAGTAGAAGGACATGCTATTTGTTTACATCCATTAGTTTGTAAGGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAGCGGGAATCGCCGAGGTATTTGTGCAAATAGGTATAATCCATATAATCGCACAAATTCGAAAAATGAAAGAATTGCCGATAATAATTCTAAATATACGAAAGAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGGTGGCGGCTAGACCAACGTGTTATTACTTCAAGAGAAACTCCCATCGAAGTTCACTATGAGTCTTTGGGTACCTCTCATGAGATTTATGGACACTATGTAATAGTAAGAAGTATAAAAAAAGAAGTTCTTTGTATATATGTTCGAACCACAGTTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCTTCAGCTAGGACCACAGTTCCTGAATTTCTATGCGAATCAAGATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCAGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACCGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAAGTTTGATTTTGGAAAAACACCATCATTATGGTAATGTACACGCGGTAGAAAAATTACGCCAATCTATTGAGATATGGTATGCTACAAGTGAATATTTGCGACAAGAAATGAGTCCTAATTTTAGGATGACGGACCCTTTTAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACACATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGTCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTCGAAATAAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACGTCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGGTCATTCTCTTTCGGTTGAAGGACGATATTTTTCTAGTCTTTCAGTAA

Protein Analysis

933

Amino Acids

105.52

Weight (kDa)

8.91

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 82 - 200 1.6e-30 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 202 - 249 3.2e-07 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_2 PF00623 260 - 342 4.4e-26 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 347 - 607 3.1e-14 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 638 - 702 5.6e-14 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 717 - 903 2.3e-39 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 1370
AccB1I GGYRCC 3 cut(s) 850, 1370, 2297
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 1119
AccII CGCG 3 cut(s) 684, 1913, 2407
AclWI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
AcoI YGGCCR 1 cut(s) 2542
AcsI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
AcuI CTGAAG 2 cut(s) 794, 1503
AcyI GRCGYC 1 cut(s) 851
AfiI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
AflIII ACRYGT 1 cut(s) 1318
AgeI ACCGGT 1 cut(s) 2654
AgsI TTSAA 7 cut(s) 569, 1332, 2132, 2258, 2267, 2471, 2770
AjnI CCWGG 2 cut(s) 436, 2565
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 3 cut(s) 310, 587, 1053
AlwI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
AlwNI CAGNNNCTG 1 cut(s) 1538
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 1011, 1647, 2542
ApoI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
ArsI GACNNNNNNTTYG 6 cut(s) 897, 929, 1586, 1618, 2736, 2768
AseI ATTAAT 2 cut(s) 363, 1712
AsiGI ACCGGT 1 cut(s) 2654
Asp700I GAANNNNTTC 2 cut(s) 807, 2262
Asp718I GGTACC 1 cut(s) 1370
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
AsuC2I CCSGG 1 cut(s) 2622
AsuII TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
BaeGI GKGCMC 1 cut(s) 2627
BaeI ACNNNNGTAYC 2 cut(s) 2199, 2232
BamHI GGATCC 2 cut(s) 1063, 2098
BanI GGYRCC 3 cut(s) 850, 1370, 2297
BanII GRGCYC 4 cut(s) 356, 1104, 2191, 2552
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 356
BccI CCATC 6 cut(s) 271, 329, 953, 1352, 1673, 1899
BceAI ACGGC 1 cut(s) 2402
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 595, 629
BciT130I CCWGG 2 cut(s) 438, 2567
BciVI GTATCC 2 cut(s) 478, 2200
BcnI CCSGG 1 cut(s) 2622
BcoDI GTCTC 3 cut(s) 310, 587, 1053
BfaI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
BfmI CTRYAG 3 cut(s) 373, 2586, 2679
BfoI RGCGCY 1 cut(s) 854
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 2 cut(s) 478, 2200
BglII AGATCT 1 cut(s) 428
BisI GCNGC 1 cut(s) 1307
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 1308
Bme1390I CCNGG 3 cut(s) 438, 2567, 2622
Bme18I GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
BmrFI CCNGG 3 cut(s) 438, 2567, 2622
BmsI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
BpiI GAAGAC 1 cut(s) 208
BplI GAGNNNNNCTC 2 cut(s) 2539, 2571
BpmI CTGGAG 2 cut(s) 90, 1035
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BpuEI CTTGAG 3 cut(s) 52, 987, 2043
BpuMI CCSGG 1 cut(s) 2622
Bsa29I ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsaAI YACGTR 2 cut(s) 2144, 2282
BsaBI GATNNNNATC 2 cut(s) 963, 2738
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BsaWI WCCGGW 2 cut(s) 1299, 2654
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
Bse118I RCCGGY 2 cut(s) 790, 2654
Bse1I ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
Bse3DI GCAATG 1 cut(s) 2412
Bse8I GATNNNNATC 2 cut(s) 963, 2738
BseBI CCWGG 2 cut(s) 438, 2567
BseCI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BseDI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BseGI GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BseJI GATNNNNATC 2 cut(s) 963, 2738
BseLI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
BseMI GCAATG 1 cut(s) 2412
BseMII CTCAG 1 cut(s) 1628
BseNI ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
BseSI GKGCMC 1 cut(s) 2627
BsgI GTGCAG 2 cut(s) 2718, 2729
Bsh1236I CGCG 3 cut(s) 684, 1913, 2407
BshFI GGCC 3 cut(s) 1013, 1649, 2544
BshNI GGYRCC 3 cut(s) 850, 1370, 2297
BshTI ACCGGT 1 cut(s) 2654
BshVI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
BslFI GGGAC 2 cut(s) 539, 2698
BslI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
BsmAI GTCTC 3 cut(s) 310, 587, 1053
BsmFI GGGAC 2 cut(s) 539, 2698
BsnI GGCC 3 cut(s) 1013, 1649, 2544
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 308
Bsp119I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
Bsp1286I GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
Bsp1407I TGTACA 1 cut(s) 1906
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp68I TCGCGA 1 cut(s) 684
BspANI GGCC 3 cut(s) 1013, 1649, 2544
BspCNI CTCAG 1 cut(s) 1627
BspDI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BspFNI CGCG 3 cut(s) 684, 1913, 2407
BspHI TCATGA 1 cut(s) 1378
BspMAI CTGCAG 1 cut(s) 2683
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
BspT104I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BspT107I GGYRCC 3 cut(s) 850, 1370, 2297
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 2412
BsrFI RCCGGY 2 cut(s) 790, 2654
BsrGI TGTACA 1 cut(s) 1906
BsrI ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
BssAI RCCGGY 2 cut(s) 790, 2654
BssECI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 1 cut(s) 1495
Bst2UI CCWGG 2 cut(s) 438, 2567
Bst4CI ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
Bst6I CTCTTC 2 cut(s) 373, 1522
BstACI GRCGYC 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 1906
BstBAI YACGTR 2 cut(s) 2144, 2282
BstBI TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BstC8I GCNNGC 1 cut(s) 2191
BstDEI CTNAG 4 cut(s) 240, 765, 1614, 1836
BstDSI CCRYGG 2 cut(s) 168, 508
BstF5I GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BstFNI CGCG 3 cut(s) 684, 1913, 2407
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstMAI GTCTC 3 cut(s) 310, 587, 1053
BstMWI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
BstNI CCWGG 2 cut(s) 438, 2567
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 3 cut(s) 436, 2565, 2620
BstSFI CTRYAG 3 cut(s) 373, 2586, 2679
BstSLI GKGCMC 1 cut(s) 2627
BstSNI TACGTA 1 cut(s) 2282
BstUI CGCG 3 cut(s) 684, 1913, 2407
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 4 cut(s) 428, 1063, 2098, 2523
BstXI CCANNNNNNTGG 1 cut(s) 1898
BstYI RGATCY 4 cut(s) 428, 1063, 2098, 2523
Bsu15I ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsuI GTATCC 2 cut(s) 478, 2200
BsuRI GGCC 3 cut(s) 1013, 1649, 2544
BsuTUI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BtgI CCRYGG 2 cut(s) 168, 508
BtgZI GCGATG 1 cut(s) 2422
BtsCI GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BtsIMutI CAGTG 2 cut(s) 1584, 2712
BtuMI TCGCGA 1 cut(s) 684
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 2191
CaiI CAGNNNCTG 1 cut(s) 1538
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 2 cut(s) 790, 2654
Cfr13I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
ClaI ATCGAT 4 cut(s) 408, 667, 1677, 2455
CseI GACGC 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 2565
CspAI ACCGGT 1 cut(s) 2654
CviAII CATG 6 cut(s) 229, 908, 980, 1379, 2558, 2687
DdeI CTNAG 4 cut(s) 240, 765, 1614, 1836
DinI GGCGCC 1 cut(s) 852
EaeI YGGCCR 1 cut(s) 2542
Eam1104I CTCTTC 2 cut(s) 373, 1522
EarI CTCTTC 2 cut(s) 373, 1522
EciI GGCGGA 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 354
Eco105I TACGTA 1 cut(s) 2282
Eco130I CCWWGG 1 cut(s) 1495
Eco24I GRGCYC 4 cut(s) 356, 1104, 2191, 2552
Eco31I GGTCTC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 2237
Eco47I GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
Eco53kI GAGCTC 1 cut(s) 354
Eco57I CTGAAG 2 cut(s) 794, 1503
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 436, 2565
EcoRV GATATC 1 cut(s) 2237
EcoT14I CCWWGG 1 cut(s) 1495
EcoT22I ATGCAT 2 cut(s) 2056, 2414
EcoT38I GRGCYC 4 cut(s) 356, 1104, 2191, 2552
EgeI GGCGCC 1 cut(s) 852
EheI GGCGCC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 1495
FaeI CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1413, 1445
FaqI GGGAC 2 cut(s) 539, 2698
FatI CATG 6 cut(s) 228, 907, 979, 1378, 2557, 2686
FauI CCCGC 3 cut(s) 1112, 2198, 2593
FauNDI CATATG 2 cut(s) 1035, 2022
Fnu4HI GCNGC 1 cut(s) 1307
FokI GGATG 8 cut(s) 289, 816, 909, 1733, 1780, 2008, 2240, 2347
FriOI GRGCYC 4 cut(s) 356, 1104, 2191, 2552
Fsp4HI GCNGC 1 cut(s) 1307
FspBI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
GlaI GCGC 1 cut(s) 852
GluI GCNGC 1 cut(s) 1307
GsuI CTGGAG 2 cut(s) 90, 1035
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 3 cut(s) 1013, 1649, 2544
HapII CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
HgaI GACGC 1 cut(s) 234
HhaI GCGC 1 cut(s) 853
Hin1I GRCGYC 1 cut(s) 851
Hin1II CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HpaII CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
Hpy166II GTNNAC 9 cut(s) 168, 344, 512, 544, 920, 1019, 1354, 1909, 2065
Hpy188I TCNGA 9 cut(s) 86, 330, 358, 393, 559, 1727, 2098, 2227, 2306
Hpy8I GTNNAC 9 cut(s) 168, 344, 512, 544, 920, 1019, 1354, 1909, 2065
Hpy99I CGWCG 1 cut(s) 2227
HpyAV CCTTC 8 cut(s) 155, 563, 660, 896, 1842, 2141, 2729, 2764
HpyCH4III ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
HpyCH4IV ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
HpyCH4V TGCA 9 cut(s) 823, 862, 1142, 1247, 2054, 2412, 2681, 2699, 2710
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
HpyF3I CTNAG 4 cut(s) 240, 765, 1614, 1836
HpySE526I ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
Hsp92I GRCGYC 1 cut(s) 851
Hsp92II CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
HspAI GCGC 1 cut(s) 851
KasI GGCGCC 1 cut(s) 850
KpnI GGTACC 1 cut(s) 1374
LmnI GCTCC 6 cut(s) 71, 1253, 1302, 2039, 2186, 2547
LweI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
MabI ACCWGGT 1 cut(s) 2565
MaeI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
MaeII ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
MaeIII GTNAC 1 cut(s) 2180
MbiI CCGCTC 1 cut(s) 1119
MboII GAAGA 9 cut(s) 10, 170, 197, 213, 293, 390, 594, 1496, 1509
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 4 cut(s) 428, 1063, 2098, 2523
MhlI GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
Mly113I GGCGCC 1 cut(s) 851
MlyI GAGTC 7 cut(s) 589, 1281, 1370, 1583, 1759, 1990, 2329
MmeI TCCRAC 3 cut(s) 178, 2076, 2205
Mph1103I ATGCAT 2 cut(s) 2056, 2414
MroXI GAANNNNTTC 2 cut(s) 807, 2262
MslI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
MspA1I CMGCKG 1 cut(s) 1618
MspI CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
MspR9I CCNGG 3 cut(s) 438, 2567, 2622
MunI CAATTG 1 cut(s) 1248
MvaI CCWGG 2 cut(s) 438, 2567
MvnI CGCG 3 cut(s) 684, 1913, 2407
MwoI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
NarI GGCGCC 1 cut(s) 851
NciI CCSGG 1 cut(s) 2622
NdeI CATATG 2 cut(s) 1035, 2022
NlaIII CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
NmeAIII GCCGAG 2 cut(s) 1154, 1532
NruI TCGCGA 1 cut(s) 684
NsiI ATGCAT 2 cut(s) 2056, 2414
NspI RCATGY 1 cut(s) 911
NspV TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
PagI TCATGA 1 cut(s) 1378
PaqCI CACCTGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 807, 2262
PfeI GAWTC 6 cut(s) 504, 1039, 1123, 1553, 2457, 2702
PflFI GACNNNGTC 1 cut(s) 2567
PflMI CCANNNNNTGG 1 cut(s) 1058
PinAI ACCGGT 1 cut(s) 2654
PkrI GCNGC 1 cut(s) 1308
PleI GAGTC 7 cut(s) 589, 1280, 1369, 1583, 1759, 1989, 2328
PluTI GGCGCC 1 cut(s) 854
PpsI GAGTC 7 cut(s) 589, 1280, 1369, 1583, 1759, 1989, 2328
Ppu21I YACGTR 2 cut(s) 2144, 2282
PpuMI RGGWCCY 1 cut(s) 647
PshBI ATTAAT 2 cut(s) 363, 1712
Psp124BI GAGCTC 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 436, 2565
PspGI CCWGG 2 cut(s) 436, 2565
PspPI GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
PspPPI RGGWCCY 1 cut(s) 647
PsrI GAACNNNNNNTAC 4 cut(s) 2485, 2517, 2635, 2667
PstI CTGCAG 1 cut(s) 2683
PstNI CAGNNNCTG 1 cut(s) 1538
PsuI RGATCY 4 cut(s) 428, 1063, 2098, 2523
PsyI GACNNNGTC 1 cut(s) 2567
RruI TCGCGA 1 cut(s) 684
RseI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
SacI GAGCTC 1 cut(s) 356
SatI GCNGC 1 cut(s) 1307
Sau96I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
SchI GAGTC 7 cut(s) 589, 1281, 1370, 1583, 1759, 1990, 2329
ScrFI CCNGG 3 cut(s) 438, 2567, 2622
SduI GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
SexAI ACCWGGT 1 cut(s) 2565
SfaNI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
SfcI CTRYAG 3 cut(s) 373, 2586, 2679
SfoI GGCGCC 1 cut(s) 852
SfuI TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
SinI GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
SmiMI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
SmlI CTYRAG 3 cut(s) 31, 1002, 2058
SmoI CTYRAG 3 cut(s) 31, 1002, 2058
SnaBI TACGTA 1 cut(s) 2282
SspDI GGCGCC 1 cut(s) 850
SspI AATATT 1 cut(s) 1964
SspMI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
SstI GAGCTC 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 436, 2565, 2620
StyI CCWWGG 1 cut(s) 1495
TaaI ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
TaiI ACGT 5 cut(s) 1321, 2146, 2225, 2284, 2519
TatI WGTACW 2 cut(s) 1906, 2062
TauI GCSGC 1 cut(s) 1309
TfiI GAWTC 6 cut(s) 504, 1039, 1123, 1553, 2457, 2702
TscAI CASTG 2 cut(s) 1591, 2712
TspGWI ACGGA 4 cut(s) 497, 1063, 2015, 2199
TspRI CASTG 2 cut(s) 1591, 2712
Tth111I GACNNNGTC 1 cut(s) 2567
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
VspI ATTAAT 2 cut(s) 363, 1712
XapI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 2366
XmnI GAANNNNTTC 2 cut(s) 807, 2262
XspI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
Zsp2I ATGCAT 2 cut(s) 2056, 2414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.