RLG00000000283

DNA-directed 5'-3' RNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004622
Physical Location & Seq
Reverse (-)
354390 .. 357922
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000283

Sequence Viewer

Length: 2799 bp
ATGAACTGGTGCTTGATCTTCTTTGGCAACTTGAGCTTCGATACATTTCGAAATCGAGAAATTTCTACTGGAGCAGGTGCTATCCGAGAACAATTAGCCGATCTGGATTTGAGAATTATTATAGATTATTCGCTGTTAGAATGGAAAGAATTGGGGGAAGAAGGGTCCACGGGGAATGAATGGGAAGATCGAAAGGTTGGAAGACGAAAGGATTTTTTGGTTAGACGCATGGAGTTGGCTAAGCATTTTATTCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTATTACCAGTTCTTCCGCCCGAGTTGAGACCAATCATTCAGATAGATGGGGGTAAACTAATGAGCTCGGATATTAATGAACTCTATAGAAGAGTTATTTATCGGAACAATACTCTTATCGATCTCTTAACAACAAGTAGATCTACGCCAGGAGAATTAGTAATGTGTCAGGAGAAATTGGTACAAGAAGCTGTGGATACACTTCTTGATAATGGAATCCGTGGACAACCAATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGTAATTGAAGGAAAAGAGGGAAGATTTCGTGAGACTCTGCTTGGCAAACGGGTCGATTATTCGGGGCGTTCTGTCATTGTTGTAGGTCCTTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATAGCAATAGAGCTTTTCCAGACATTTGTAATTCGTGGTTTAATTAGACAGCATTTTGCCTCGAATATAGGAGTGGCTAAGAGTAAAATTCGGGAAAAAGAGCCGGTTGTATGGGAAATACTTCAGGAAGTTATGCAAGGGCATCCCGTATTGCTGAATAGGGCGCCTACTCTGCATAGATTAGGCATACAGGCGTTCCAGCCCATTTTAGTAGAAGGACATGCTATTTGTTTACATCCATTAGTTTGTAAGGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAGCGGGAATCGCCGAGGTATTTGTGCAAATAGGTATAATCCATATAATCGCACAAATTCGAAAAATGAAAGAATTGCCGATAATAATTCTAAATATACGAAAGAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGGTGGCGGCTAGACCAACGTGTTATTACTTCAAGAGAAACTCCCATCGAAGTTCACTATGAGTCTTTGGGTACCTCTCATGAGATTTATGGACACTATGTAATAGTAAGAAGTATAAAAAAAGAAGTTCTTTGTATATATGTTCGAACCACAGTTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCTTCAGCTAGGACCACAGTTCCTGAATTTCTATGCGAATCAAGATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCAGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACCGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAAGTTTGATTTTGGAAAAACACCATCATTATGGTAATGTACACGCGGTAGAAAAATTACGCCAATCTATTGAGATATGGTATGCTACAAGTGAATATTTGCGACAAGAAATGAGTCCTAATTTTAGGATGACGGACCCTTTTAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACACATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGTCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTCGAAATAAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACGTCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGGTCATTCTCTTTCGGTTGAAGGACGATATTTTTCTAGTCTTTCAGTAA

Protein Analysis

933

Amino Acids

105.52

Weight (kDa)

8.91

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 82 - 200 1.6e-30 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 202 - 249 3.2e-07 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_2 PF00623 260 - 342 4.4e-26 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 347 - 607 3.1e-14 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 638 - 702 5.6e-14 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 717 - 903 2.3e-39 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 1370
AccB1I GGYRCC 3 cut(s) 850, 1370, 2297
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 1119
AccII CGCG 3 cut(s) 684, 1913, 2407
AclWI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
AcoI YGGCCR 1 cut(s) 2542
AcsI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
AcuI CTGAAG 2 cut(s) 794, 1503
AcyI GRCGYC 1 cut(s) 851
AfiI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
AflIII ACRYGT 1 cut(s) 1318
AgeI ACCGGT 1 cut(s) 2654
AgsI TTSAA 7 cut(s) 569, 1332, 2132, 2258, 2267, 2471, 2770
AjnI CCWGG 2 cut(s) 436, 2565
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 3 cut(s) 310, 587, 1053
AlwI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
AlwNI CAGNNNCTG 1 cut(s) 1538
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 1011, 1647, 2542
ApoI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
ArsI GACNNNNNNTTYG 6 cut(s) 897, 929, 1586, 1618, 2736, 2768
AseI ATTAAT 2 cut(s) 363, 1712
AsiGI ACCGGT 1 cut(s) 2654
Asp700I GAANNNNTTC 2 cut(s) 807, 2262
Asp718I GGTACC 1 cut(s) 1370
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
AsuC2I CCSGG 1 cut(s) 2622
AsuII TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
BaeGI GKGCMC 1 cut(s) 2627
BaeI ACNNNNGTAYC 2 cut(s) 2199, 2232
BamHI GGATCC 2 cut(s) 1063, 2098
BanI GGYRCC 3 cut(s) 850, 1370, 2297
BanII GRGCYC 4 cut(s) 356, 1104, 2191, 2552
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 356
BccI CCATC 6 cut(s) 271, 329, 953, 1352, 1673, 1899
BceAI ACGGC 1 cut(s) 2402
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 595, 629
BciT130I CCWGG 2 cut(s) 438, 2567
BciVI GTATCC 2 cut(s) 478, 2200
BcnI CCSGG 1 cut(s) 2622
BcoDI GTCTC 3 cut(s) 310, 587, 1053
BfaI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
BfmI CTRYAG 3 cut(s) 373, 2586, 2679
BfoI RGCGCY 1 cut(s) 854
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 2 cut(s) 478, 2200
BglII AGATCT 1 cut(s) 428
BisI GCNGC 1 cut(s) 1307
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 1308
Bme1390I CCNGG 3 cut(s) 438, 2567, 2622
Bme18I GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
BmrFI CCNGG 3 cut(s) 438, 2567, 2622
BmsI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
BpiI GAAGAC 1 cut(s) 208
BplI GAGNNNNNCTC 2 cut(s) 2539, 2571
BpmI CTGGAG 2 cut(s) 90, 1035
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BpuEI CTTGAG 3 cut(s) 52, 987, 2043
BpuMI CCSGG 1 cut(s) 2622
Bsa29I ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsaAI YACGTR 2 cut(s) 2144, 2282
BsaBI GATNNNNATC 2 cut(s) 963, 2738
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BsaWI WCCGGW 2 cut(s) 1299, 2654
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
Bse118I RCCGGY 2 cut(s) 790, 2654
Bse1I ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
Bse3DI GCAATG 1 cut(s) 2412
Bse8I GATNNNNATC 2 cut(s) 963, 2738
BseBI CCWGG 2 cut(s) 438, 2567
BseCI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BseDI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BseGI GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BseJI GATNNNNATC 2 cut(s) 963, 2738
BseLI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
BseMI GCAATG 1 cut(s) 2412
BseMII CTCAG 1 cut(s) 1628
BseNI ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
BseSI GKGCMC 1 cut(s) 2627
BsgI GTGCAG 2 cut(s) 2718, 2729
Bsh1236I CGCG 3 cut(s) 684, 1913, 2407
BshFI GGCC 3 cut(s) 1013, 1649, 2544
BshNI GGYRCC 3 cut(s) 850, 1370, 2297
BshTI ACCGGT 1 cut(s) 2654
BshVI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
BslFI GGGAC 2 cut(s) 539, 2698
BslI CCNNNNNNNGG 5 cut(s) 1058, 1617, 1838, 2330, 2558
BsmAI GTCTC 3 cut(s) 310, 587, 1053
BsmFI GGGAC 2 cut(s) 539, 2698
BsnI GGCC 3 cut(s) 1013, 1649, 2544
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 308
Bsp119I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
Bsp1286I GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
Bsp1407I TGTACA 1 cut(s) 1906
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp68I TCGCGA 1 cut(s) 684
BspANI GGCC 3 cut(s) 1013, 1649, 2544
BspCNI CTCAG 1 cut(s) 1627
BspDI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BspFNI CGCG 3 cut(s) 684, 1913, 2407
BspHI TCATGA 1 cut(s) 1378
BspMAI CTGCAG 1 cut(s) 2683
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 7 cut(s) 972, 1058, 1071, 2093, 2106, 2531, 2741
BspT104I TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BspT107I GGYRCC 3 cut(s) 850, 1370, 2297
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 2412
BsrFI RCCGGY 2 cut(s) 790, 2654
BsrGI TGTACA 1 cut(s) 1906
BsrI ACTGG 5 cut(s) 11, 73, 296, 1650, 2015
BssAI RCCGGY 2 cut(s) 790, 2654
BssECI CCNNGG 5 cut(s) 168, 508, 1129, 1495, 2305
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 1 cut(s) 1495
Bst2UI CCWGG 2 cut(s) 438, 2567
Bst4CI ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
Bst6I CTCTTC 2 cut(s) 373, 1522
BstACI GRCGYC 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 1906
BstBAI YACGTR 2 cut(s) 2144, 2282
BstBI TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
BstC8I GCNNGC 1 cut(s) 2191
BstDEI CTNAG 4 cut(s) 240, 765, 1614, 1836
BstDSI CCRYGG 2 cut(s) 168, 508
BstF5I GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BstFNI CGCG 3 cut(s) 684, 1913, 2407
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstMAI GTCTC 3 cut(s) 310, 587, 1053
BstMWI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
BstNI CCWGG 2 cut(s) 438, 2567
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 3 cut(s) 436, 2565, 2620
BstSFI CTRYAG 3 cut(s) 373, 2586, 2679
BstSLI GKGCMC 1 cut(s) 2627
BstSNI TACGTA 1 cut(s) 2282
BstUI CGCG 3 cut(s) 684, 1913, 2407
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 4 cut(s) 428, 1063, 2098, 2523
BstXI CCANNNNNNTGG 1 cut(s) 1898
BstYI RGATCY 4 cut(s) 428, 1063, 2098, 2523
Bsu15I ATCGAT 4 cut(s) 408, 667, 1677, 2455
BsuI GTATCC 2 cut(s) 478, 2200
BsuRI GGCC 3 cut(s) 1013, 1649, 2544
BsuTUI ATCGAT 4 cut(s) 408, 667, 1677, 2455
BtgI CCRYGG 2 cut(s) 168, 508
BtgZI GCGATG 1 cut(s) 2422
BtsCI GGATG 8 cut(s) 282, 829, 922, 1746, 1793, 2001, 2233, 2340
BtsIMutI CAGTG 2 cut(s) 1584, 2712
BtuMI TCGCGA 1 cut(s) 684
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 2191
CaiI CAGNNNCTG 1 cut(s) 1538
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 2 cut(s) 790, 2654
Cfr13I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
ClaI ATCGAT 4 cut(s) 408, 667, 1677, 2455
CseI GACGC 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 2565
CspAI ACCGGT 1 cut(s) 2654
CviAII CATG 6 cut(s) 229, 908, 980, 1379, 2558, 2687
DdeI CTNAG 4 cut(s) 240, 765, 1614, 1836
DinI GGCGCC 1 cut(s) 852
EaeI YGGCCR 1 cut(s) 2542
Eam1104I CTCTTC 2 cut(s) 373, 1522
EarI CTCTTC 2 cut(s) 373, 1522
EciI GGCGGA 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 354
Eco105I TACGTA 1 cut(s) 2282
Eco130I CCWWGG 1 cut(s) 1495
Eco24I GRGCYC 4 cut(s) 356, 1104, 2191, 2552
Eco31I GGTCTC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 2237
Eco47I GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
Eco53kI GAGCTC 1 cut(s) 354
Eco57I CTGAAG 2 cut(s) 794, 1503
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 436, 2565
EcoRV GATATC 1 cut(s) 2237
EcoT14I CCWWGG 1 cut(s) 1495
EcoT22I ATGCAT 2 cut(s) 2056, 2414
EcoT38I GRGCYC 4 cut(s) 356, 1104, 2191, 2552
EgeI GGCGCC 1 cut(s) 852
EheI GGCGCC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 1495
FaeI CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1413, 1445
FaqI GGGAC 2 cut(s) 539, 2698
FatI CATG 6 cut(s) 228, 907, 979, 1378, 2557, 2686
FauI CCCGC 3 cut(s) 1112, 2198, 2593
FauNDI CATATG 2 cut(s) 1035, 2022
Fnu4HI GCNGC 1 cut(s) 1307
FokI GGATG 8 cut(s) 289, 816, 909, 1733, 1780, 2008, 2240, 2347
FriOI GRGCYC 4 cut(s) 356, 1104, 2191, 2552
Fsp4HI GCNGC 1 cut(s) 1307
FspBI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
GlaI GCGC 1 cut(s) 852
GluI GCNGC 1 cut(s) 1307
GsuI CTGGAG 2 cut(s) 90, 1035
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 3 cut(s) 1013, 1649, 2544
HapII CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
HgaI GACGC 1 cut(s) 234
HhaI GCGC 1 cut(s) 853
Hin1I GRCGYC 1 cut(s) 851
Hin1II CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HpaII CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
Hpy166II GTNNAC 9 cut(s) 168, 344, 512, 544, 920, 1019, 1354, 1909, 2065
Hpy188I TCNGA 9 cut(s) 86, 330, 358, 393, 559, 1727, 2098, 2227, 2306
Hpy8I GTNNAC 9 cut(s) 168, 344, 512, 544, 920, 1019, 1354, 1909, 2065
Hpy99I CGWCG 1 cut(s) 2227
HpyAV CCTTC 8 cut(s) 155, 563, 660, 896, 1842, 2141, 2729, 2764
HpyCH4III ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
HpyCH4IV ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
HpyCH4V TGCA 9 cut(s) 823, 862, 1142, 1247, 2054, 2412, 2681, 2699, 2710
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
HpyF3I CTNAG 4 cut(s) 240, 765, 1614, 1836
HpySE526I ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
Hsp92I GRCGYC 1 cut(s) 851
Hsp92II CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
HspAI GCGC 1 cut(s) 851
KasI GGCGCC 1 cut(s) 850
KpnI GGTACC 1 cut(s) 1374
LmnI GCTCC 6 cut(s) 71, 1253, 1302, 2039, 2186, 2547
LweI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
MabI ACCWGGT 1 cut(s) 2565
MaeI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
MaeII ACGT 5 cut(s) 1318, 2143, 2222, 2281, 2516
MaeIII GTNAC 1 cut(s) 2180
MbiI CCGCTC 1 cut(s) 1119
MboII GAAGA 9 cut(s) 10, 170, 197, 213, 293, 390, 594, 1496, 1509
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 4 cut(s) 428, 1063, 2098, 2523
MhlI GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
Mly113I GGCGCC 1 cut(s) 851
MlyI GAGTC 7 cut(s) 589, 1281, 1370, 1583, 1759, 1990, 2329
MmeI TCCRAC 3 cut(s) 178, 2076, 2205
Mph1103I ATGCAT 2 cut(s) 2056, 2414
MroXI GAANNNNTTC 2 cut(s) 807, 2262
MslI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
MspA1I CMGCKG 1 cut(s) 1618
MspI CCGG 6 cut(s) 791, 1300, 2342, 2545, 2621, 2655
MspR9I CCNGG 3 cut(s) 438, 2567, 2622
MunI CAATTG 1 cut(s) 1248
MvaI CCWGG 2 cut(s) 438, 2567
MvnI CGCG 3 cut(s) 684, 1913, 2407
MwoI GCNNNNNNNGC 7 cut(s) 33, 859, 1099, 1125, 1253, 1303, 1646
NarI GGCGCC 1 cut(s) 851
NciI CCSGG 1 cut(s) 2622
NdeI CATATG 2 cut(s) 1035, 2022
NlaIII CATG 6 cut(s) 232, 911, 983, 1382, 2561, 2690
NmeAIII GCCGAG 2 cut(s) 1154, 1532
NruI TCGCGA 1 cut(s) 684
NsiI ATGCAT 2 cut(s) 2056, 2414
NspI RCATGY 1 cut(s) 911
NspV TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
PagI TCATGA 1 cut(s) 1378
PaqCI CACCTGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 807, 2262
PfeI GAWTC 6 cut(s) 504, 1039, 1123, 1553, 2457, 2702
PflFI GACNNNGTC 1 cut(s) 2567
PflMI CCANNNNNTGG 1 cut(s) 1058
PinAI ACCGGT 1 cut(s) 2654
PkrI GCNGC 1 cut(s) 1308
PleI GAGTC 7 cut(s) 589, 1280, 1369, 1583, 1759, 1989, 2328
PluTI GGCGCC 1 cut(s) 854
PpsI GAGTC 7 cut(s) 589, 1280, 1369, 1583, 1759, 1989, 2328
Ppu21I YACGTR 2 cut(s) 2144, 2282
PpuMI RGGWCCY 1 cut(s) 647
PshBI ATTAAT 2 cut(s) 363, 1712
Psp124BI GAGCTC 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 436, 2565
PspGI CCWGG 2 cut(s) 436, 2565
PspPI GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
PspPPI RGGWCCY 1 cut(s) 647
PsrI GAACNNNNNNTAC 4 cut(s) 2485, 2517, 2635, 2667
PstI CTGCAG 1 cut(s) 2683
PstNI CAGNNNCTG 1 cut(s) 1538
PsuI RGATCY 4 cut(s) 428, 1063, 2098, 2523
PsyI GACNNNGTC 1 cut(s) 2567
RruI TCGCGA 1 cut(s) 684
RseI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
SacI GAGCTC 1 cut(s) 356
SatI GCNGC 1 cut(s) 1307
Sau96I GGNCC 7 cut(s) 165, 647, 1012, 1527, 1647, 2002, 2402
SchI GAGTC 7 cut(s) 589, 1281, 1370, 1583, 1759, 1990, 2329
ScrFI CCNGG 3 cut(s) 438, 2567, 2622
SduI GDGCHC 5 cut(s) 356, 1104, 2191, 2552, 2627
SexAI ACCWGGT 1 cut(s) 2565
SfaNI GCATC 7 cut(s) 838, 1234, 1846, 2063, 2218, 2328, 2399
SfcI CTRYAG 3 cut(s) 373, 2586, 2679
SfoI GGCGCC 1 cut(s) 852
SfuI TTCGAA 6 cut(s) 49, 253, 1175, 1444, 2422, 2491
SinI GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
SmiMI CAYNNNNRTG 3 cut(s) 669, 1896, 2655
SmlI CTYRAG 3 cut(s) 31, 1002, 2058
SmoI CTYRAG 3 cut(s) 31, 1002, 2058
SnaBI TACGTA 1 cut(s) 2282
SspDI GGCGCC 1 cut(s) 850
SspI AATATT 1 cut(s) 1964
SspMI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
SstI GAGCTC 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 436, 2565, 2620
StyI CCWWGG 1 cut(s) 1495
TaaI ACNGT 5 cut(s) 530, 1453, 1534, 2302, 2752
TaiI ACGT 5 cut(s) 1321, 2146, 2225, 2284, 2519
TatI WGTACW 2 cut(s) 1906, 2062
TauI GCSGC 1 cut(s) 1309
TfiI GAWTC 6 cut(s) 504, 1039, 1123, 1553, 2457, 2702
TscAI CASTG 2 cut(s) 1591, 2712
TspGWI ACGGA 4 cut(s) 497, 1063, 2015, 2199
TspRI CASTG 2 cut(s) 1591, 2712
Tth111I GACNNNGTC 1 cut(s) 2567
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 5 cut(s) 165, 647, 1527, 2002, 2402
VspI ATTAAT 2 cut(s) 363, 1712
XapI RAATTY 5 cut(s) 60, 774, 1171, 1541, 2349
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 2366
XmnI GAANNNNTTC 2 cut(s) 807, 2262
XspI CTAG 6 cut(s) 1310, 1524, 1751, 1847, 2043, 2786
Zsp2I ATGCAT 2 cut(s) 2056, 2414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.